Plaque/focus immunoassay: a simple method for detecting antiviral monoclonal or other antibodies and viral antigens in cells
Identifieur interne : 000437 ( Istex/Corpus ); précédent : 000436; suivant : 000438Plaque/focus immunoassay: a simple method for detecting antiviral monoclonal or other antibodies and viral antigens in cells
Auteurs : Georg Pauli ; Jens-Peter Gregersen ; Hanns LudwigSource :
- Journal of Immunological Methods [ 0022-1759 ] ; 1984.
English descriptors
- Teeft :
- Antibody, Comparable results, Different fixatives, Fixation, Fixative, Fixative solution, Focus immunoassay, Glutaraldehyde fixation, Growth medium, Horseradish peroxidase, Immune reaction step, Immunoassay, Infectious units, Large scale screening, Microtitre, Microtitre plate, Microtitre plates, Monoclonal antibodies, Plaque, Plaque immunoassay, Secondary antibodies, Substrate solution, Test serum dilutions, Uninfected cells, Viral antigens.
Abstract
Abstract: A new, simple enzyme-linked immunosorbent assay (ELISA) is described which is performed directly on infected and fixed cell cultures in microtitre plates. It permits large scale screening of antiviral monoclonal antibodies and differentiation of specific antibodies from those usually responsible for high background reactions in other ELISA techniques. Time consuming purification of antigens is thus avoided.The plaque/focus immunoassay is also applicable to titration of antibodies in patients' sera and antigens in lytically or non-lytically virus-infected cells. It may also be used to localize antigens in different cell compartments. This immunoassay requires no special equipment and results may be evaluated either with the naked eye or using a light microscope.
Url:
DOI: 10.1016/0022-1759(84)90301-6
Links to Exploration step
ISTEX:FF9244C8CA94116E1DE47356BA3DFA146211FA5ALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Plaque/focus immunoassay: a simple method for detecting antiviral monoclonal or other antibodies and viral antigens in cells</title><author><name sortKey="Pauli, Georg" sort="Pauli, Georg" uniqKey="Pauli G" first="Georg" last="Pauli">Georg Pauli</name><affiliation><mods:affiliation>Institut für Virologie, Freie Universität Berlin, Nordufer 20, D-1000 Berlin 65, F.R.G.</mods:affiliation></affiliation></author><author><name sortKey="Gregersen, Jens Peter" sort="Gregersen, Jens Peter" uniqKey="Gregersen J" first="Jens-Peter" last="Gregersen">Jens-Peter Gregersen</name><affiliation><mods:affiliation>Institut für Virologie, Freie Universität Berlin, Nordufer 20, D-1000 Berlin 65, F.R.G.</mods:affiliation></affiliation></author><author><name sortKey="Ludwig, Hanns" sort="Ludwig, Hanns" uniqKey="Ludwig H" first="Hanns" last="Ludwig">Hanns Ludwig</name><affiliation><mods:affiliation>Institut für Virologie, Freie Universität Berlin, Nordufer 20, D-1000 Berlin 65, F.R.G.</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:FF9244C8CA94116E1DE47356BA3DFA146211FA5A</idno><date when="1984" year="1984">1984</date><idno type="doi">10.1016/0022-1759(84)90301-6</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-XJ6HGQ7V-N/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000437</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000437</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Plaque/focus immunoassay: a simple method for detecting antiviral monoclonal or other antibodies and viral antigens in cells</title><author><name sortKey="Pauli, Georg" sort="Pauli, Georg" uniqKey="Pauli G" first="Georg" last="Pauli">Georg Pauli</name><affiliation><mods:affiliation>Institut für Virologie, Freie Universität Berlin, Nordufer 20, D-1000 Berlin 65, F.R.G.</mods:affiliation></affiliation></author><author><name sortKey="Gregersen, Jens Peter" sort="Gregersen, Jens Peter" uniqKey="Gregersen J" first="Jens-Peter" last="Gregersen">Jens-Peter Gregersen</name><affiliation><mods:affiliation>Institut für Virologie, Freie Universität Berlin, Nordufer 20, D-1000 Berlin 65, F.R.G.</mods:affiliation></affiliation></author><author><name sortKey="Ludwig, Hanns" sort="Ludwig, Hanns" uniqKey="Ludwig H" first="Hanns" last="Ludwig">Hanns Ludwig</name><affiliation><mods:affiliation>Institut für Virologie, Freie Universität Berlin, Nordufer 20, D-1000 Berlin 65, F.R.G.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Immunological Methods</title><title level="j" type="abbrev">JIM</title><idno type="ISSN">0022-1759</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1984">1984</date><biblScope unit="volume">74</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="337">337</biblScope><biblScope unit="page" to="344">344</biblScope></imprint><idno type="ISSN">0022-1759</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-1759</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Antibody</term><term>Comparable results</term><term>Different fixatives</term><term>Fixation</term><term>Fixative</term><term>Fixative solution</term><term>Focus immunoassay</term><term>Glutaraldehyde fixation</term><term>Growth medium</term><term>Horseradish peroxidase</term><term>Immune reaction step</term><term>Immunoassay</term><term>Infectious units</term><term>Large scale screening</term><term>Microtitre</term><term>Microtitre plate</term><term>Microtitre plates</term><term>Monoclonal antibodies</term><term>Plaque</term><term>Plaque immunoassay</term><term>Secondary antibodies</term><term>Substrate solution</term><term>Test serum dilutions</term><term>Uninfected cells</term><term>Viral antigens</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A new, simple enzyme-linked immunosorbent assay (ELISA) is described which is performed directly on infected and fixed cell cultures in microtitre plates. It permits large scale screening of antiviral monoclonal antibodies and differentiation of specific antibodies from those usually responsible for high background reactions in other ELISA techniques. Time consuming purification of antigens is thus avoided.The plaque/focus immunoassay is also applicable to titration of antibodies in patients' sera and antigens in lytically or non-lytically virus-infected cells. It may also be used to localize antigens in different cell compartments. This immunoassay requires no special equipment and results may be evaluated either with the naked eye or using a light microscope.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>immunoassay</json:string><json:string>fixative</json:string><json:string>fixation</json:string><json:string>microtitre</json:string><json:string>microtitre plates</json:string><json:string>monoclonal antibodies</json:string><json:string>focus immunoassay</json:string><json:string>fixative solution</json:string><json:string>substrate solution</json:string><json:string>glutaraldehyde fixation</json:string><json:string>comparable results</json:string><json:string>microtitre plate</json:string><json:string>plaque immunoassay</json:string><json:string>test serum dilutions</json:string><json:string>horseradish peroxidase</json:string><json:string>secondary antibodies</json:string><json:string>infectious units</json:string><json:string>viral antigens</json:string><json:string>uninfected cells</json:string><json:string>large scale screening</json:string><json:string>growth medium</json:string><json:string>different fixatives</json:string><json:string>immune reaction step</json:string><json:string>plaque</json:string><json:string>antibody</json:string></teeft></keywords><author><json:item><name>Georg Pauli</name><affiliations><json:string>Institut für Virologie, Freie Universität Berlin, Nordufer 20, D-1000 Berlin 65, F.R.G.</json:string></affiliations></json:item><json:item><name>Jens-Peter Gregersen</name><affiliations><json:string>Institut für Virologie, Freie Universität Berlin, Nordufer 20, D-1000 Berlin 65, F.R.G.</json:string></affiliations></json:item><json:item><name>Hanns Ludwig</name><affiliations><json:string>Institut für Virologie, Freie Universität Berlin, Nordufer 20, D-1000 Berlin 65, F.R.G.</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>enzyme immunoassay</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>monoclonal antibody</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>viral antigens</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>virus-specific antibodies</value></json:item></subject><arkIstex>ark:/67375/6H6-XJ6HGQ7V-N</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: A new, simple enzyme-linked immunosorbent assay (ELISA) is described which is performed directly on infected and fixed cell cultures in microtitre plates. It permits large scale screening of antiviral monoclonal antibodies and differentiation of specific antibodies from those usually responsible for high background reactions in other ELISA techniques. Time consuming purification of antigens is thus avoided.The plaque/focus immunoassay is also applicable to titration of antibodies in patients' sera and antigens in lytically or non-lytically virus-infected cells. It may also be used to localize antigens in different cell compartments. 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It permits large scale screening of antiviral monoclonal antibodies and differentiation of specific antibodies from those usually responsible for high background reactions in other ELISA techniques. Time consuming purification of antigens is thus avoided.</ce:simple-para><ce:simple-para>The plaque/focus immunoassay is also applicable to titration of antibodies in patients' sera and antigens in lytically or non-lytically virus-infected cells. It may also be used to localize antigens in different cell compartments. This immunoassay requires no special equipment and results may be evaluated either with the naked eye or using a light microscope.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>enzyme immunoassay</ce:text></ce:keyword><ce:keyword><ce:text>monoclonal antibody</ce:text></ce:keyword><ce:keyword><ce:text>viral antigens</ce:text></ce:keyword><ce:keyword><ce:text>virus-specific antibodies</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Plaque/focus immunoassay: a simple method for detecting antiviral monoclonal or other antibodies and viral antigens in cells</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Plaque/focus immunoassay: a simple method for detecting antiviral monoclonal or other antibodies and viral antigens in cells</title></titleInfo><name type="personal"><namePart type="given">Georg</namePart><namePart type="family">Pauli</namePart><affiliation>Institut für Virologie, Freie Universität Berlin, Nordufer 20, D-1000 Berlin 65, F.R.G.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jens-Peter</namePart><namePart type="family">Gregersen</namePart><affiliation>Institut für Virologie, Freie Universität Berlin, Nordufer 20, D-1000 Berlin 65, F.R.G.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hanns</namePart><namePart type="family">Ludwig</namePart><affiliation>Institut für Virologie, Freie Universität Berlin, Nordufer 20, D-1000 Berlin 65, F.R.G.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1984</dateIssued><copyrightDate encoding="w3cdtf">1984</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: A new, simple enzyme-linked immunosorbent assay (ELISA) is described which is performed directly on infected and fixed cell cultures in microtitre plates. It permits large scale screening of antiviral monoclonal antibodies and differentiation of specific antibodies from those usually responsible for high background reactions in other ELISA techniques. Time consuming purification of antigens is thus avoided.The plaque/focus immunoassay is also applicable to titration of antibodies in patients' sera and antigens in lytically or non-lytically virus-infected cells. It may also be used to localize antigens in different cell compartments. 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