TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions
Identifieur interne : 001420 ( Main/Merge ); précédent : 001419; suivant : 001421TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions
Auteurs : M. Godet [France] ; R. L'Haridon ; J.-F. Vautherot ; H. LaudeSource :
- Virology : (New York, NY) [ 0042-6822 ] ; 1992.
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- Pascal (Inist)
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Abstract
The coding potential of the open reading frame ORF4 (82 amino acids) of transmissible gastroenteritis virus (TGEV) has been confirmed by expression using a baculovirus vector. Five monoclonal antibodies (MAbs) raised against the 10K recombinant product immunoprecipitated a polypeptide of a similar size in TGEV-infected cells. Immunofluorescence assays performed both on insect and mammalian cells revealed that ORF4 was a membrane-associated protein, a finding consistent with the prediction of a membrane-spanning segment in ORF4 sequence
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Pascal:92-0403071Le document en format XML
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<s2>78350 Jouy-en-Josas</s2>
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<country>France</country>
<placeName><region type="region" nuts="2">Île-de-France</region>
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<author><name sortKey="L Haridon, R" sort="L Haridon, R" uniqKey="L Haridon R" first="R." last="L'Haridon">R. L'Haridon</name>
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<author><name sortKey="Vautherot, J F" sort="Vautherot, J F" uniqKey="Vautherot J" first="J.-F." last="Vautherot">J.-F. Vautherot</name>
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<author><name sortKey="Laude, H" sort="Laude, H" uniqKey="Laude H" first="H." last="Laude">H. Laude</name>
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<author><name sortKey="Godet, M" sort="Godet, M" uniqKey="Godet M" first="M." last="Godet">M. Godet</name>
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<author><name sortKey="L Haridon, R" sort="L Haridon, R" uniqKey="L Haridon R" first="R." last="L'Haridon">R. L'Haridon</name>
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<author><name sortKey="Vautherot, J F" sort="Vautherot, J F" uniqKey="Vautherot J" first="J.-F." last="Vautherot">J.-F. Vautherot</name>
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<series><title level="j" type="main">Virology : (New York, NY)</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aminoacid sequence</term>
<term>Antigenic determinant</term>
<term>Gene expression</term>
<term>Membrane protein</term>
<term>Monoclonal antibody</term>
<term>Open reading frame</term>
<term>Porcine transmissible gastroenteritis virus</term>
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<keywords scheme="Pascal" xml:lang="fr"><term>Virus gastroentérite transmissible porc</term>
<term>Cadre lecture ouvert</term>
<term>Protéine membranaire</term>
<term>Expression génique</term>
<term>Déterminant antigénique</term>
<term>Séquence aminoacide</term>
<term>Anticorps monoclonal</term>
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<front><div type="abstract" xml:lang="en">The coding potential of the open reading frame ORF4 (82 amino acids) of transmissible gastroenteritis virus (TGEV) has been confirmed by expression using a baculovirus vector. Five monoclonal antibodies (MAbs) raised against the 10K recombinant product immunoprecipitated a polypeptide of a similar size in TGEV-infected cells. Immunofluorescence assays performed both on insect and mammalian cells revealed that ORF4 was a membrane-associated protein, a finding consistent with the prediction of a membrane-spanning segment in ORF4 sequence</div>
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