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TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions

Identifieur interne : 001420 ( Main/Merge ); précédent : 001419; suivant : 001421

TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions

Auteurs : M. Godet [France] ; R. L'Haridon ; J.-F. Vautherot ; H. Laude

Source :

RBID : Pascal:92-0403071

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English descriptors

Abstract

The coding potential of the open reading frame ORF4 (82 amino acids) of transmissible gastroenteritis virus (TGEV) has been confirmed by expression using a baculovirus vector. Five monoclonal antibodies (MAbs) raised against the 10K recombinant product immunoprecipitated a polypeptide of a similar size in TGEV-infected cells. Immunofluorescence assays performed both on insect and mammalian cells revealed that ORF4 was a membrane-associated protein, a finding consistent with the prediction of a membrane-spanning segment in ORF4 sequence

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Pascal:92-0403071

Le document en format XML

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