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TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions

Identifieur interne : 000065 ( PascalFrancis/Corpus ); précédent : 000064; suivant : 000066

TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions

Auteurs : M. Godet ; R. L'Haridon ; J.-F. Vautherot ; H. Laude

Source :

RBID : Pascal:92-0403071

Descripteurs français

English descriptors

Abstract

The coding potential of the open reading frame ORF4 (82 amino acids) of transmissible gastroenteritis virus (TGEV) has been confirmed by expression using a baculovirus vector. Five monoclonal antibodies (MAbs) raised against the 10K recombinant product immunoprecipitated a polypeptide of a similar size in TGEV-infected cells. Immunofluorescence assays performed both on insect and mammalian cells revealed that ORF4 was a membrane-associated protein, a finding consistent with the prediction of a membrane-spanning segment in ORF4 sequence

Notice en format standard (ISO 2709)

Pour connaître la documentation sur le format Inist Standard.

pA  
A01 01  1    @0 0042-6822
A02 01      @0 VIRLAX
A03   1    @0 Virology : (NY, NY)
A05       @2 188
A06       @2 2
A08 01  1  ENG  @1 TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions
A11 01  1    @1 GODET (M.)
A11 02  1    @1 L'HARIDON (R.)
A11 03  1    @1 VAUTHEROT (J.-F.)
A11 04  1    @1 LAUDE (H.)
A14 01      @1 INRA, unité virologie immunologie moléculaires @2 78350 Jouy-en-Josas @3 FRA
A20       @1 666-675
A21       @1 1992
A23 01      @0 ENG
A43 01      @1 INIST @2 7801 @5 354000027997660270
A44       @0 0000
A45       @0 1 p. 1/2
A47 01  1    @0 92-0403071
A60       @1 P
A61       @0 A
A64   1    @0 Virology : (New York, NY)
A66 01      @0 USA
C01 01    ENG  @0 The coding potential of the open reading frame ORF4 (82 amino acids) of transmissible gastroenteritis virus (TGEV) has been confirmed by expression using a baculovirus vector. Five monoclonal antibodies (MAbs) raised against the 10K recombinant product immunoprecipitated a polypeptide of a similar size in TGEV-infected cells. Immunofluorescence assays performed both on insect and mammalian cells revealed that ORF4 was a membrane-associated protein, a finding consistent with the prediction of a membrane-spanning segment in ORF4 sequence
C02 01  X    @0 002A05C03
C03 01  X  FRE  @0 Virus gastroentérite transmissible porc @2 NW
C03 01  X  ENG  @0 Porcine transmissible gastroenteritis virus @2 NW
C03 01  X  SPA  @0 Porcine transmissible gastroenteritis virus @2 NW
C03 02  X  FRE  @0 Cadre lecture ouvert
C03 02  X  ENG  @0 Open reading frame
C03 02  X  SPA  @0 Cuadro lectura abierto
C03 03  X  FRE  @0 Protéine membranaire
C03 03  X  ENG  @0 Membrane protein
C03 03  X  SPA  @0 Proteína membranar
C03 04  X  FRE  @0 Expression génique
C03 04  X  ENG  @0 Gene expression
C03 04  X  SPA  @0 Expresión genética
C03 05  X  FRE  @0 Déterminant antigénique
C03 05  X  ENG  @0 Antigenic determinant
C03 05  X  SPA  @0 Determinante antigénico
C03 06  X  FRE  @0 Séquence aminoacide
C03 06  X  ENG  @0 Aminoacid sequence
C03 06  X  SPA  @0 Secuencia aminoácido
C03 07  X  FRE  @0 Anticorps monoclonal
C03 07  X  ENG  @0 Monoclonal antibody
C03 07  X  SPA  @0 Anticuerpo monoclonal
C07 01  X  FRE  @0 Coronavirus @2 NW
C07 01  X  ENG  @0 Coronavirus @2 NW
C07 01  X  SPA  @0 Coronavirus @2 NW
C07 02  X  FRE  @0 Coronaviridae @2 NW
C07 02  X  ENG  @0 Coronaviridae @2 NW
C07 02  X  SPA  @0 Coronaviridae @2 NW
C07 03  X  FRE  @0 Virus @2 NW
C07 03  X  ENG  @0 Virus @2 NW
C07 03  X  SPA  @0 Virus @2 NW
N21       @1 243

Format Inist (serveur)

NO : PASCAL 92-0403071 INIST
ET : TGEV corona virus ORF4 encodes a membrane protein that is incorporated into virions
AU : GODET (M.); L'HARIDON (R.); VAUTHEROT (J.-F.); LAUDE (H.)
AF : INRA, unité virologie immunologie moléculaires/78350 Jouy-en-Josas/France
DT : Publication en série; Niveau analytique
SO : Virology : (New York, NY); ISSN 0042-6822; Coden VIRLAX; Etats-Unis; Da. 1992; Vol. 188; No. 2; Pp. 666-675; Bibl. 1 p. 1/2
LA : Anglais
EA : The coding potential of the open reading frame ORF4 (82 amino acids) of transmissible gastroenteritis virus (TGEV) has been confirmed by expression using a baculovirus vector. Five monoclonal antibodies (MAbs) raised against the 10K recombinant product immunoprecipitated a polypeptide of a similar size in TGEV-infected cells. Immunofluorescence assays performed both on insect and mammalian cells revealed that ORF4 was a membrane-associated protein, a finding consistent with the prediction of a membrane-spanning segment in ORF4 sequence
CC : 002A05C03
FD : Virus gastroentérite transmissible porc; Cadre lecture ouvert; Protéine membranaire; Expression génique; Déterminant antigénique; Séquence aminoacide; Anticorps monoclonal
FG : Coronavirus; Coronaviridae; Virus
ED : Porcine transmissible gastroenteritis virus; Open reading frame; Membrane protein; Gene expression; Antigenic determinant; Aminoacid sequence; Monoclonal antibody
EG : Coronavirus; Coronaviridae; Virus
SD : Porcine transmissible gastroenteritis virus; Cuadro lectura abierto; Proteína membranar; Expresión genética; Determinante antigénico; Secuencia aminoácido; Anticuerpo monoclonal
LO : INIST-7801.354000027997660270
ID : 92-0403071

Links to Exploration step

Pascal:92-0403071

Le document en format XML

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