Persistent memory CD4+ and CD8+ T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen
Identifieur interne : 000E85 ( Main/Merge ); précédent : 000E84; suivant : 000E86Persistent memory CD4+ and CD8+ T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen
Auteurs : Litao Yang [République populaire de Chine] ; Hui Peng [République populaire de Chine] ; Zhaoling Zhu [République populaire de Chine] ; Gang Li [République populaire de Chine] ; Zitong Huang [République populaire de Chine] ; Zhixin Zhao [République populaire de Chine] ; Richard A. Koup [États-Unis] ; Robert T. Bailer [États-Unis] ; Changyou Wu [République populaire de Chine]Source :
- The Journal of general virology [ 0022-1317 ] ; 2007.
Abstract
The membrane (M) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is a major glycoprotein with multiple biological functions. In this study, we found that memory T cells against M protein were persistent in recovered SARS patients by detecting gamma interferon (IFN-
Url:
DOI: 10.1099/vir.0.82839-0
PubMed: 17872527
PubMed Central: 2362397
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PMC:2362397Le document en format XML
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and CD8<sup>+</sup>
T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen</title>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Persistent memory CD4<sup>+</sup>
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T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen</title>
<author><name sortKey="Yang, Litao" sort="Yang, Litao" uniqKey="Yang L" first="Litao" last="Yang">Litao Yang</name>
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<author><name sortKey="Peng, Hui" sort="Peng, Hui" uniqKey="Peng H" first="Hui" last="Peng">Hui Peng</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
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<author><name sortKey="Zhu, Zhaoling" sort="Zhu, Zhaoling" uniqKey="Zhu Z" first="Zhaoling" last="Zhu">Zhaoling Zhu</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
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<author><name sortKey="Li, Gang" sort="Li, Gang" uniqKey="Li G" first="Gang" last="Li">Gang Li</name>
<affiliation wicri:level="1"><nlm:aff id="A2">Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
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<author><name sortKey="Huang, Zitong" sort="Huang, Zitong" uniqKey="Huang Z" first="Zitong" last="Huang">Zitong Huang</name>
<affiliation wicri:level="1"><nlm:aff id="A3">Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
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<author><name sortKey="Zhao, Zhixin" sort="Zhao, Zhixin" uniqKey="Zhao Z" first="Zhixin" last="Zhao">Zhixin Zhao</name>
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<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
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<author><name sortKey="Koup, Richard A" sort="Koup, Richard A" uniqKey="Koup R" first="Richard A." last="Koup">Richard A. Koup</name>
<affiliation wicri:level="1"><nlm:aff id="A4">Vaccine Research Center, NIAID/NIH, Bethesda, MD 20892, USA</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Vaccine Research Center, NIAID/NIH, Bethesda, MD 20892</wicri:regionArea>
<wicri:noRegion>MD 20892</wicri:noRegion>
</affiliation>
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<author><name sortKey="Bailer, Robert T" sort="Bailer, Robert T" uniqKey="Bailer R" first="Robert T." last="Bailer">Robert T. Bailer</name>
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<wicri:noRegion>MD 20892</wicri:noRegion>
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<author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name>
<affiliation wicri:level="1"><nlm:aff id="A1">Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
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<series><title level="j">The Journal of general virology</title>
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<front><div type="abstract" xml:lang="en"><p id="P1">The membrane (M) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is a major glycoprotein with multiple biological functions. In this study, we found that memory T cells against M protein were persistent in recovered SARS patients by detecting gamma interferon (IFN-<italic>γ</italic>
) production using ELISA and ELISpot assays. Flow cytometric analysis showed that both CD4<sup>+</sup>
and CD8<sup>+</sup>
T cells were involved in cellular responses to SARS-CoV M antigen. Furthermore, memory CD8<sup>+</sup>
T cells displayed an effector memory cell phenotype expressing CD45RO<sup>−</sup>
CCR7<sup>−</sup>
CD62L<sup>−</sup>
. In contrast, the majority of IFN-<italic>γ</italic>
<sup>+</sup>
CD4<sup>+</sup>
T cells were central memory cells with the expression of CD45RO<sup>+</sup>
CCR7<sup>+</sup>
CD62L<sup>−</sup>
. The epitope screening from 30 synthetic overlapping peptides that cover the entire SARS-CoV M protein identified four human T-cell immunodominant peptides, p21−44, p65−91, p117−140 and p200−220. All four immunodominant peptides could elicit cellular immunity with a predominance of CD8<sup>+</sup>
T-cell response. This data may have important implication for developing SARS vaccines.</p>
</div>
</front>
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