CovidV1, Pmc, Corpus, bibRecord, 000481

Persistent memory CD4+ and CD8+ T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen

Identifieur interne : 000481 ( Pmc/Corpus ); précédent : 000480; suivant : 000482

Persistent memory CD4+ and CD8+ T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen

Auteurs : Litao Yang ; Hui Peng ; Zhaoling Zhu ; Gang Li ; Zitong Huang ; Zhixin Zhao ; Richard A. Koup ; Robert T. Bailer ; Changyou Wu

Source :

The Journal of general virology [ 0022-1317 ] ; 2007.

RBID : PMC:2362397

Abstract

The membrane (M) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is a major glycoprotein with multiple biological functions. In this study, we found that memory T cells against M protein were persistent in recovered SARS patients by detecting gamma interferon (IFN-γ) production using ELISA and ELISpot assays. Flow cytometric analysis showed that both CD4⁺ and CD8⁺ T cells were involved in cellular responses to SARS-CoV M antigen. Furthermore, memory CD8⁺ T cells displayed an effector memory cell phenotype expressing CD45RO⁻ CCR7⁻ CD62L⁻. In contrast, the majority of IFN-γ⁺ CD4⁺ T cells were central memory cells with the expression of CD45RO⁺ CCR7⁺ CD62L⁻. The epitope screening from 30 synthetic overlapping peptides that cover the entire SARS-CoV M protein identified four human T-cell immunodominant peptides, p21−44, p65−91, p117−140 and p200−220. All four immunodominant peptides could elicit cellular immunity with a predominance of CD8⁺ T-cell response. This data may have important implication for developing SARS vaccines.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2362397

DOI: 10.1099/vir.0.82839-0
PubMed: 17872527
PubMed Central: 2362397

Links to Exploration step

PMC:2362397

Le document en format XML

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 and CD8<sup>+</sup>
 T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen</title>
<author><name sortKey="Yang, Litao" sort="Yang, Litao" uniqKey="Yang L" first="Litao" last="Yang">Litao Yang</name>
<affiliation><nlm:aff id="A1">Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Peng, Hui" sort="Peng, Hui" uniqKey="Peng H" first="Hui" last="Peng">Hui Peng</name>
<affiliation><nlm:aff id="A1">Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhu, Zhaoling" sort="Zhu, Zhaoling" uniqKey="Zhu Z" first="Zhaoling" last="Zhu">Zhaoling Zhu</name>
<affiliation><nlm:aff id="A1">Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Gang" sort="Li, Gang" uniqKey="Li G" first="Gang" last="Li">Gang Li</name>
<affiliation><nlm:aff id="A2">Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Huang, Zitong" sort="Huang, Zitong" uniqKey="Huang Z" first="Zitong" last="Huang">Zitong Huang</name>
<affiliation><nlm:aff id="A3">Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhao, Zhixin" sort="Zhao, Zhixin" uniqKey="Zhao Z" first="Zhixin" last="Zhao">Zhixin Zhao</name>
<affiliation><nlm:aff id="A2">Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Koup, Richard A" sort="Koup, Richard A" uniqKey="Koup R" first="Richard A." last="Koup">Richard A. Koup</name>
<affiliation><nlm:aff id="A4">Vaccine Research Center, NIAID/NIH, Bethesda, MD 20892, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bailer, Robert T" sort="Bailer, Robert T" uniqKey="Bailer R" first="Robert T." last="Bailer">Robert T. Bailer</name>
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</affiliation>
</author>
<author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name>
<affiliation><nlm:aff id="A1">Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089, China</nlm:aff>
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<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Persistent memory CD4<sup>+</sup>
 and CD8<sup>+</sup>
 T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen</title>
<author><name sortKey="Yang, Litao" sort="Yang, Litao" uniqKey="Yang L" first="Litao" last="Yang">Litao Yang</name>
<affiliation><nlm:aff id="A1">Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Peng, Hui" sort="Peng, Hui" uniqKey="Peng H" first="Hui" last="Peng">Hui Peng</name>
<affiliation><nlm:aff id="A1">Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhu, Zhaoling" sort="Zhu, Zhaoling" uniqKey="Zhu Z" first="Zhaoling" last="Zhu">Zhaoling Zhu</name>
<affiliation><nlm:aff id="A1">Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Gang" sort="Li, Gang" uniqKey="Li G" first="Gang" last="Li">Gang Li</name>
<affiliation><nlm:aff id="A2">Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Huang, Zitong" sort="Huang, Zitong" uniqKey="Huang Z" first="Zitong" last="Huang">Zitong Huang</name>
<affiliation><nlm:aff id="A3">Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhao, Zhixin" sort="Zhao, Zhixin" uniqKey="Zhao Z" first="Zhixin" last="Zhao">Zhixin Zhao</name>
<affiliation><nlm:aff id="A2">Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Koup, Richard A" sort="Koup, Richard A" uniqKey="Koup R" first="Richard A." last="Koup">Richard A. Koup</name>
<affiliation><nlm:aff id="A4">Vaccine Research Center, NIAID/NIH, Bethesda, MD 20892, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Bailer, Robert T" sort="Bailer, Robert T" uniqKey="Bailer R" first="Robert T." last="Bailer">Robert T. Bailer</name>
<affiliation><nlm:aff id="A4">Vaccine Research Center, NIAID/NIH, Bethesda, MD 20892, USA</nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name>
<affiliation><nlm:aff id="A1">Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089, China</nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">The Journal of general virology</title>
<idno type="ISSN">0022-1317</idno>
<idno type="eISSN">1465-2099</idno>
<imprint><date when="2007">2007</date>
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<front><div type="abstract" xml:lang="en"><p id="P1">The membrane (M) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is a major glycoprotein with multiple biological functions. In this study, we found that memory T cells against M protein were persistent in recovered SARS patients by detecting gamma interferon (IFN-<italic>γ</italic>
) production using ELISA and ELISpot assays. Flow cytometric analysis showed that both CD4<sup>+</sup>
 and CD8<sup>+</sup>
 T cells were involved in cellular responses to SARS-CoV M antigen. Furthermore, memory CD8<sup>+</sup>
 T cells displayed an effector memory cell phenotype expressing CD45RO<sup>−</sup>
 CCR7<sup>−</sup>
 CD62L<sup>−</sup>
. In contrast, the majority of IFN-<italic>γ</italic>
<sup>+</sup>
 CD4<sup>+</sup>
 T cells were central memory cells with the expression of CD45RO<sup>+</sup>
 CCR7<sup>+</sup>
 CD62L<sup>−</sup>
. The epitope screening from 30 synthetic overlapping peptides that cover the entire SARS-CoV M protein identified four human T-cell immunodominant peptides, p21−44, p65−91, p117−140 and p200−220. All four immunodominant peptides could elicit cellular immunity with a predominance of CD8<sup>+</sup>
 T-cell response. This data may have important implication for developing SARS vaccines.</p>
</div>
</front>
</TEI>
<pmc article-type="research-article"><pmc-comment>The publisher of this article does not allow downloading of the full text in XML form.</pmc-comment>
  <pmc-dir>properties manuscript</pmc-dir>
  <front><journal-meta><journal-id journal-id-type="nlm-journal-id">0077340</journal-id>
<journal-id journal-id-type="pubmed-jr-id">4747</journal-id>
<journal-id journal-id-type="nlm-ta">J Gen Virol</journal-id>
<journal-id journal-id-type="iso-abbrev">J. Gen. Virol.</journal-id>
<journal-title-group><journal-title>The Journal of general virology</journal-title>
</journal-title-group>
<issn pub-type="ppub">0022-1317</issn>
<issn pub-type="epub">1465-2099</issn>
</journal-meta>
<article-meta><article-id pub-id-type="pmid">17872527</article-id>
<article-id pub-id-type="pmc">2362397</article-id>
<article-id pub-id-type="doi">10.1099/vir.0.82839-0</article-id>
<article-id pub-id-type="manuscript">NIHMS44947</article-id>
<article-categories><subj-group subj-group-type="heading"><subject>Article</subject>
</subj-group>
</article-categories>
<title-group><article-title>Persistent memory CD4<sup>+</sup>
 and CD8<sup>+</sup>
 T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen</article-title>
</title-group>
<contrib-group><contrib contrib-type="author"><name><surname>Yang</surname>
<given-names>Litao</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Peng</surname>
<given-names>Hui</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zhu</surname>
<given-names>Zhaoling</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Li</surname>
<given-names>Gang</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Huang</surname>
<given-names>Zitong</given-names>
</name>
<xref ref-type="aff" rid="A3">3</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Zhao</surname>
<given-names>Zhixin</given-names>
</name>
<xref ref-type="aff" rid="A2">2</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Koup</surname>
<given-names>Richard A.</given-names>
</name>
<xref ref-type="aff" rid="A4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Bailer</surname>
<given-names>Robert T.</given-names>
</name>
<xref ref-type="aff" rid="A4">4</xref>
</contrib>
<contrib contrib-type="author"><name><surname>Wu</surname>
<given-names>Changyou</given-names>
</name>
<xref ref-type="aff" rid="A1">1</xref>
</contrib>
</contrib-group>
<aff id="A1"><label>1</label>
Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, Guangzhou 510089, China</aff>
<aff id="A2"><label>2</label>
Third Affiliated Hospital of Sun Yat-sen University, Guangzhou 510630, China</aff>
<aff id="A3"><label>3</label>
Second Affiliated Hospital of Sun Yat-sen University, Guangzhou 510120, China</aff>
<aff id="A4"><label>4</label>
Vaccine Research Center, NIAID/NIH, Bethesda, MD 20892, USA</aff>
<author-notes><corresp id="CR1">Correspondence Changyou Wu <email>changyou_wu@yahoo.com</email>
</corresp>
</author-notes>
<pub-date pub-type="nihms-submitted"><day>3</day>
<month>4</month>
<year>2008</year>
</pub-date>
<pub-date pub-type="ppub"><month>10</month>
<year>2007</year>
</pub-date>
<pub-date pub-type="pmc-release"><day>01</day>
<month>10</month>
<year>2008</year>
</pub-date>
<volume>88</volume>
<issue>Pt 10</issue>
<fpage>2740</fpage>
<lpage>2748</lpage>
<pmc-comment>elocation-id from pubmed: 10.1099/vir.0.82839-0</pmc-comment>
      <abstract><p id="P1">The membrane (M) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) is a major glycoprotein with multiple biological functions. In this study, we found that memory T cells against M protein were persistent in recovered SARS patients by detecting gamma interferon (IFN-<italic>γ</italic>
) production using ELISA and ELISpot assays. Flow cytometric analysis showed that both CD4<sup>+</sup>
 and CD8<sup>+</sup>
 T cells were involved in cellular responses to SARS-CoV M antigen. Furthermore, memory CD8<sup>+</sup>
 T cells displayed an effector memory cell phenotype expressing CD45RO<sup>−</sup>
 CCR7<sup>−</sup>
 CD62L<sup>−</sup>
. In contrast, the majority of IFN-<italic>γ</italic>
<sup>+</sup>
 CD4<sup>+</sup>
 T cells were central memory cells with the expression of CD45RO<sup>+</sup>
 CCR7<sup>+</sup>
 CD62L<sup>−</sup>
. The epitope screening from 30 synthetic overlapping peptides that cover the entire SARS-CoV M protein identified four human T-cell immunodominant peptides, p21−44, p65−91, p117−140 and p200−220. All four immunodominant peptides could elicit cellular immunity with a predominance of CD8<sup>+</sup>
 T-cell response. This data may have important implication for developing SARS vaccines.</p>
</abstract>
</article-meta>
</front>
</pmc>
</record>

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Persistent memory CD4+ and CD8+ T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen

Persistent memory CD4+ and CD8+ T-cell responses in recovered severe acute respiratory syndrome (SARS) patients to SARS coronavirus M antigen

Source :

Abstract

Links to Exploration step

Le document en format XML

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