Syncytium formation is induced in the murine neuroblastoma cell cultures which produce pathogenic type G proteins of the rabies virus
Identifieur interne : 000695 ( Istex/Corpus ); précédent : 000694; suivant : 000696Syncytium formation is induced in the murine neuroblastoma cell cultures which produce pathogenic type G proteins of the rabies virus
Auteurs : Kinjiro Morimoto ; Ya-Jin Ni ; Akihiko KawaiSource :
- Virology [ 0042-6822 ] ; 1992.
English descriptors
- Teeft :
- Amino, Amino acid, Amino acid substitution, Arginine, Butyrate, Cdna, Cell, Cell clones, Cell cultures, Cell density, Cell fusion, Cell lines, Cell surface, Cell types, Clone, Culture fluids, Culture medium, Dietzschold, Expression vector, Fusogenic, Gene expression, Giant cell formation, Glutamine, Glycoprotein, Host cells, Kawai, Monoclonal antibodies, Morimoto, Morphological changes, Multinucleated cells, Murine, Murine neuroblastoma, Mutagenesis technique, Mutant, Neuroblastoma, Neuroblastoma cells, Neuronal, Neuronal cells, Nonpathogenic, Nonpathogenic virus, Nonpathogenic viruses, Other hand, Pathogenic, Pathogenic virus, Protein, Protein synthesis, Putative fusogenic domain, Rabies, Rabies virus, Rabies virus glycoprotein, Relative amounts, Retroviral expression vector, Sodium, Sodium butyrate, Sodium butyrate treatment, Sodium cell cultures, Staining solution, Surface expression, Syncytium, Syncytium formation, Unpublished observations, Untreated, Viral, Viral invasion, Virology, Virulent, Virus, Wunner.
Abstract
Abstract: We investigated comparatively the interactions of host cells with two types of rabies virus G protein, an avirulent type G (Gln) and a virulent type G (Arg) protein, having glutamine and arginine at position 333, respectively. For this purpose, we established four types of cell lines (referred to as G(Gln)-NA, G(Arg)-NA, G(Gln)-BHK, and G(Arg)-BHK cells, respectively) by transfecting either the G(Gln)-cDNA or G(Arg)-cDNA into two kinds of cells, murine neuroblastoma C1300 (clone NA) and nonneuronal BHK-21. Both G(Gln)-NA and G(Arg)-NA cells produced G proteins when they were treated with 5 mM sodium butyrate, but only G(Arg)-NA cells formed syncytia at the neutral pH, which was suppressed by anti-G antiserum. The sodium butyrate-treated G(Arg)-NA cells fused also with sodium butyrate-treated NA cells under coculture conditions, but neither with untreated NA cells nor with BHK-21 cells. On the other hand, both G(Gln)-BHK and G(Arg)-BHK cells constitutively produced G proteins, but no syncytium was produced at the neutral pH. G(Arg)-BHK cells, however, formed syncytia with the sodium butyrate-treated NA cells when they were cocultured. These results suggest that only G(Arg) has a potential ability to produce syncytia of NA cells regardless of cell types by which G(Arg) protein was produced and also suggest that a certain cellular factor(s) is required for the syncytium formation, the factor(s) which is lacking in BHK-21 and untreated NA cells but is produced by the sodium butyrate-treated NA cells.
Url:
DOI: 10.1016/0042-6822(92)90696-M
Links to Exploration step
ISTEX:FDAB8A348A84521FE60ABCA3E3337ED7EDCECB2DLe document en format XML
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Kawai</name><affiliation><mods:affiliation>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto, Japan</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:FDAB8A348A84521FE60ABCA3E3337ED7EDCECB2D</idno><date when="1992" year="1992">1992</date><idno type="doi">10.1016/0042-6822(92)90696-M</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-PNNGNXG8-X/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000695</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000695</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Syncytium formation is induced in the murine neuroblastoma cell cultures which produce pathogenic type G proteins of the rabies virus</title><author><name sortKey="Morimoto, Kinjiro" sort="Morimoto, Kinjiro" uniqKey="Morimoto K" first="Kinjiro" last="Morimoto">Kinjiro Morimoto</name><affiliation><mods:affiliation>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto, Japan</mods:affiliation></affiliation></author><author><name sortKey="Ni, Ya Jin" sort="Ni, Ya Jin" uniqKey="Ni Y" first="Ya-Jin" last="Ni">Ya-Jin Ni</name><affiliation><mods:affiliation>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto, Japan</mods:affiliation></affiliation></author><author><name sortKey="Kawai, Akihiko" sort="Kawai, Akihiko" uniqKey="Kawai A" first="Akihiko" last="Kawai">Akihiko Kawai</name><affiliation><mods:affiliation>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto, Japan</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno 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expression</term><term>Giant cell formation</term><term>Glutamine</term><term>Glycoprotein</term><term>Host cells</term><term>Kawai</term><term>Monoclonal antibodies</term><term>Morimoto</term><term>Morphological changes</term><term>Multinucleated cells</term><term>Murine</term><term>Murine neuroblastoma</term><term>Mutagenesis technique</term><term>Mutant</term><term>Neuroblastoma</term><term>Neuroblastoma cells</term><term>Neuronal</term><term>Neuronal cells</term><term>Nonpathogenic</term><term>Nonpathogenic virus</term><term>Nonpathogenic viruses</term><term>Other hand</term><term>Pathogenic</term><term>Pathogenic virus</term><term>Protein</term><term>Protein synthesis</term><term>Putative fusogenic domain</term><term>Rabies</term><term>Rabies virus</term><term>Rabies virus glycoprotein</term><term>Relative amounts</term><term>Retroviral expression vector</term><term>Sodium</term><term>Sodium butyrate</term><term>Sodium butyrate treatment</term><term>Sodium cell cultures</term><term>Staining solution</term><term>Surface expression</term><term>Syncytium</term><term>Syncytium formation</term><term>Unpublished observations</term><term>Untreated</term><term>Viral</term><term>Viral invasion</term><term>Virology</term><term>Virulent</term><term>Virus</term><term>Wunner</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: We investigated comparatively the interactions of host cells with two types of rabies virus G protein, an avirulent type G (Gln) and a virulent type G (Arg) protein, having glutamine and arginine at position 333, respectively. For this purpose, we established four types of cell lines (referred to as G(Gln)-NA, G(Arg)-NA, G(Gln)-BHK, and G(Arg)-BHK cells, respectively) by transfecting either the G(Gln)-cDNA or G(Arg)-cDNA into two kinds of cells, murine neuroblastoma C1300 (clone NA) and nonneuronal BHK-21. Both G(Gln)-NA and G(Arg)-NA cells produced G proteins when they were treated with 5 mM sodium butyrate, but only G(Arg)-NA cells formed syncytia at the neutral pH, which was suppressed by anti-G antiserum. The sodium butyrate-treated G(Arg)-NA cells fused also with sodium butyrate-treated NA cells under coculture conditions, but neither with untreated NA cells nor with BHK-21 cells. On the other hand, both G(Gln)-BHK and G(Arg)-BHK cells constitutively produced G proteins, but no syncytium was produced at the neutral pH. G(Arg)-BHK cells, however, formed syncytia with the sodium butyrate-treated NA cells when they were cocultured. These results suggest that only G(Arg) has a potential ability to produce syncytia of NA cells regardless of cell types by which G(Arg) protein was produced and also suggest that a certain cellular factor(s) is required for the syncytium formation, the factor(s) which is lacking in BHK-21 and untreated NA cells but is produced by the sodium butyrate-treated NA cells.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>syncytium</json:string><json:string>syncytium formation</json:string><json:string>rabies virus</json:string><json:string>clone</json:string><json:string>sodium butyrate</json:string><json:string>rabies</json:string><json:string>morimoto</json:string><json:string>viral</json:string><json:string>nonpathogenic</json:string><json:string>cell fusion</json:string><json:string>glycoprotein</json:string><json:string>arginine</json:string><json:string>kawai</json:string><json:string>neuroblastoma</json:string><json:string>glutamine</json:string><json:string>mutant</json:string><json:string>virology</json:string><json:string>cell cultures</json:string><json:string>cdna</json:string><json:string>murine</json:string><json:string>cell surface</json:string><json:string>fusogenic</json:string><json:string>sodium butyrate treatment</json:string><json:string>dietzschold</json:string><json:string>cell lines</json:string><json:string>culture medium</json:string><json:string>wunner</json:string><json:string>neuronal</json:string><json:string>neuronal cells</json:string><json:string>pathogenic</json:string><json:string>giant cell formation</json:string><json:string>untreated</json:string><json:string>viral invasion</json:string><json:string>other hand</json:string><json:string>pathogenic virus</json:string><json:string>nonpathogenic viruses</json:string><json:string>nonpathogenic virus</json:string><json:string>unpublished observations</json:string><json:string>protein synthesis</json:string><json:string>cell clones</json:string><json:string>cell types</json:string><json:string>host cells</json:string><json:string>virus</json:string><json:string>virulent</json:string><json:string>amino</json:string><json:string>gene expression</json:string><json:string>multinucleated cells</json:string><json:string>morphological changes</json:string><json:string>neuroblastoma cells</json:string><json:string>rabies virus glycoprotein</json:string><json:string>monoclonal antibodies</json:string><json:string>amino acid substitution</json:string><json:string>surface expression</json:string><json:string>putative fusogenic domain</json:string><json:string>relative amounts</json:string><json:string>sodium cell cultures</json:string><json:string>cell density</json:string><json:string>mutagenesis technique</json:string><json:string>culture fluids</json:string><json:string>staining solution</json:string><json:string>retroviral expression vector</json:string><json:string>amino acid</json:string><json:string>murine neuroblastoma</json:string><json:string>expression vector</json:string><json:string>protein</json:string><json:string>sodium</json:string><json:string>butyrate</json:string><json:string>cell</json:string></teeft></keywords><author><json:item><name>Kinjiro Morimoto</name><affiliations><json:string>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto, Japan</json:string></affiliations></json:item><json:item><name>Ya-Jin Ni</name><affiliations><json:string>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto, Japan</json:string></affiliations></json:item><json:item><name>Akihiko Kawai</name><affiliations><json:string>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto, Japan</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-PNNGNXG8-X</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: We investigated comparatively the interactions of host cells with two types of rabies virus G protein, an avirulent type G (Gln) and a virulent type G (Arg) protein, having glutamine and arginine at position 333, respectively. For this purpose, we established four types of cell lines (referred to as G(Gln)-NA, G(Arg)-NA, G(Gln)-BHK, and G(Arg)-BHK cells, respectively) by transfecting either the G(Gln)-cDNA or G(Arg)-cDNA into two kinds of cells, murine neuroblastoma C1300 (clone NA) and nonneuronal BHK-21. Both G(Gln)-NA and G(Arg)-NA cells produced G proteins when they were treated with 5 mM sodium butyrate, but only G(Arg)-NA cells formed syncytia at the neutral pH, which was suppressed by anti-G antiserum. The sodium butyrate-treated G(Arg)-NA cells fused also with sodium butyrate-treated NA cells under coculture conditions, but neither with untreated NA cells nor with BHK-21 cells. On the other hand, both G(Gln)-BHK and G(Arg)-BHK cells constitutively produced G proteins, but no syncytium was produced at the neutral pH. G(Arg)-BHK cells, however, formed syncytia with the sodium butyrate-treated NA cells when they were cocultured. These results suggest that only G(Arg) has a potential ability to produce syncytia of NA cells regardless of cell types by which G(Arg) protein was produced and also suggest that a certain cellular factor(s) is required for the syncytium formation, the factor(s) which is lacking in BHK-21 and untreated NA cells but is produced by the sodium butyrate-treated NA cells.</abstract><qualityIndicators><score>9.784</score><pdfWordCount>7472</pdfWordCount><pdfCharCount>45795</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>14</pdfPageCount><pdfPageSize>611.68 x 792.12 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>232</abstractWordCount><abstractCharCount>1531</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Syncytium formation is induced in the murine neuroblastoma cell cultures which produce pathogenic type G proteins of the rabies virus</title><pmid><json:string>1604811</json:string></pmid><pii><json:string>0042-6822(92)90696-M</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Natl. Acad. Sci. USA</title><language><json:string>unknown</json:string></language><volume>81</volume><pages><first>7683</first><last>7687</last></pages></serie><host><title>Virology</title><language><json:string>unknown</json:string></language><publicationDate>1992</publicationDate><issn><json:string>0042-6822</json:string></issn><pii><json:string>S0042-6822(00)X0422-9</json:string></pii><volume>189</volume><issue>1</issue><pages><first>203</first><last>216</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1992</json:string></date><geogName></geogName><orgName><json:string>Academic Press, Inc</json:string><json:string>Kyoto University</json:string><json:string>Shindengen Kogyo Co., Tokyo</json:string><json:string>Japan, and In</json:string><json:string>Sumitomo Bakelite Co., Tokyo</json:string><json:string>Chemo-Sero-Therapeutic Research Institute</json:string></orgName><orgName_funder><json:string>Chemo-Sero-Therapeutic Research Institute</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>S. Sakamoto</json:string><json:string>Mulligan</json:string><json:string>J. Nonaka</json:string><json:string>Y. Nagai</json:string><json:string>I. Kodera</json:string><json:string>In</json:string></persName><placeName><json:string>Kumamoto</json:string><json:string>Japan</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Dietzschold et al. (1985)</json:string><json:string>Davis et al., 198613</json:string><json:string>Naito and Matsumoto, 1978</json:string><json:string>Love et al., 1985</json:string><json:string>Morimoto et al., 1992</json:string><json:string>Pence et al., 1990</json:string><json:string>Lentz et al., 1984</json:string><json:string>McMorris and Ruddle, 1974</json:string><json:string>Coulon et al., 1982, 1983</json:string><json:string>Goodman and Engel, 1991</json:string><json:string>Mifune et al. (1982)</json:string><json:string>Lafay et al. (1991)</json:string><json:string>Seif et al., 1985</json:string><json:string>Collins et al., 1984</json:string><json:string>Davis et al. (1986a)</json:string><json:string>Richardson et al., 1986</json:string><json:string>Wunner and Dietzschold, 1987</json:string><json:string>Lentz et al. (1984)</json:string><json:string>LaMonica et al., 1987</json:string><json:string>Morimoto et al., 1989</json:string><json:string>Towbin et al., 1979</json:string><json:string>Schneider (1976)</json:string><json:string>Tuffereau et al. (1989)</json:string><json:string>Whitt et al. (1991)</json:string><json:string>Laemmli, 1970</json:string><json:string>Sellers, 1927</json:string><json:string>Prehaud et al., 1988</json:string><json:string>Schneider, 1976</json:string><json:string>Wain-Hobson et al., 1985</json:string><json:string>Dietzschold et al., 1985</json:string><json:string>Whitt et al., 1991</json:string><json:string>Kucera et al., 1985</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-PNNGNXG8-X</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1992</publicationDate><copyrightDate>1992</copyrightDate><doi><json:string>10.1016/0042-6822(92)90696-M</json:string></doi><id>FDAB8A348A84521FE60ABCA3E3337ED7EDCECB2D</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-PNNGNXG8-X/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-PNNGNXG8-X/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-PNNGNXG8-X/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Syncytium formation is induced in the murine neuroblastoma cell cultures which produce pathogenic type G proteins of the rabies virus</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1992</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Syncytium formation is induced in the murine neuroblastoma cell cultures which produce pathogenic type G proteins of the rabies virus</title><author xml:id="author-0000"><persName><forename type="first">Kinjiro</forename><surname>Morimoto</surname></persName><affiliation>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto, Japan</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Ya-Jin</forename><surname>Ni</surname></persName><affiliation>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto, Japan</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Akihiko</forename><surname>Kawai</surname></persName><affiliation>To whom correspondence and reprint requests should be addressed.</affiliation><affiliation>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto, Japan</affiliation></author><idno type="istex">FDAB8A348A84521FE60ABCA3E3337ED7EDCECB2D</idno><idno type="ark">ark:/67375/6H6-PNNGNXG8-X</idno><idno type="DOI">10.1016/0042-6822(92)90696-M</idno><idno type="PII">0042-6822(92)90696-M</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0422-9</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1992"></date><biblScope unit="volume">189</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="203">203</biblScope><biblScope unit="page" to="216">216</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1992</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: We investigated comparatively the interactions of host cells with two types of rabies virus G protein, an avirulent type G (Gln) and a virulent type G (Arg) protein, having glutamine and arginine at position 333, respectively. For this purpose, we established four types of cell lines (referred to as G(Gln)-NA, G(Arg)-NA, G(Gln)-BHK, and G(Arg)-BHK cells, respectively) by transfecting either the G(Gln)-cDNA or G(Arg)-cDNA into two kinds of cells, murine neuroblastoma C1300 (clone NA) and nonneuronal BHK-21. Both G(Gln)-NA and G(Arg)-NA cells produced G proteins when they were treated with 5 mM sodium butyrate, but only G(Arg)-NA cells formed syncytia at the neutral pH, which was suppressed by anti-G antiserum. The sodium butyrate-treated G(Arg)-NA cells fused also with sodium butyrate-treated NA cells under coculture conditions, but neither with untreated NA cells nor with BHK-21 cells. On the other hand, both G(Gln)-BHK and G(Arg)-BHK cells constitutively produced G proteins, but no syncytium was produced at the neutral pH. G(Arg)-BHK cells, however, formed syncytia with the sodium butyrate-treated NA cells when they were cocultured. These results suggest that only G(Arg) has a potential ability to produce syncytia of NA cells regardless of cell types by which G(Arg) protein was produced and also suggest that a certain cellular factor(s) is required for the syncytium formation, the factor(s) which is lacking in BHK-21 and untreated NA cells but is produced by the sodium butyrate-treated NA cells.</p></abstract></profileDesc><revisionDesc><change when="1992">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-PNNGNXG8-X/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YVIRO</jid><aid>9290696M</aid><ce:pii>0042-6822(92)90696-M</ce:pii><ce:doi>10.1016/0042-6822(92)90696-M</ce:doi><ce:copyright type="unknown" year="1992"></ce:copyright></item-info><head><ce:title>Syncytium formation is induced in the murine neuroblastoma cell cultures which produce pathogenic type G proteins of the rabies virus</ce:title><ce:author-group><ce:author><ce:given-name>Kinjiro</ce:given-name><ce:surname>Morimoto</ce:surname></ce:author><ce:author><ce:given-name>Ya-Jin</ce:given-name><ce:surname>Ni</ce:surname></ce:author><ce:author><ce:given-name>Akihiko</ce:given-name><ce:surname>Kawai</ce:surname><ce:cross-ref refid="COR1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:affiliation><ce:textfn>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto, Japan</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>1</ce:label><ce:text>To whom correspondence and reprint requests should be addressed.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="9" month="1" year="1992"></ce:date-received><ce:date-accepted day="26" month="3" year="1992"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>We investigated comparatively the interactions of host cells with two types of rabies virus G protein, an avirulent type G (Gln) and a virulent type G (Arg) protein, having glutamine and arginine at position 333, respectively. For this purpose, we established four types of cell lines (referred to as G(Gln)-NA, G(Arg)-NA, G(Gln)-BHK, and G(Arg)-BHK cells, respectively) by transfecting either the G(Gln)-cDNA or G(Arg)-cDNA into two kinds of cells, murine neuroblastoma C1300 (clone NA) and nonneuronal BHK-21. Both G(Gln)-NA and G(Arg)-NA cells produced G proteins when they were treated with 5 m<ce:italic>M</ce:italic> sodium butyrate, but only G(Arg)-NA cells formed syncytia at the neutral pH, which was suppressed by anti-G antiserum. The sodium butyrate-treated G(Arg)-NA cells fused also with sodium butyrate-treated NA cells under coculture conditions, but neither with untreated NA cells nor with BHK-21 cells. On the other hand, both G(Gln)-BHK and G(Arg)-BHK cells constitutively produced G proteins, but no syncytium was produced at the neutral pH. G(Arg)-BHK cells, however, formed syncytia with the sodium butyrate-treated NA cells when they were cocultured. These results suggest that only G(Arg) has a potential ability to produce syncytia of NA cells regardless of cell types by which G(Arg) protein was produced and also suggest that a certain cellular factor(s) is required for the syncytium formation, the factor(s) which is lacking in BHK-21 and untreated NA cells but is produced by the sodium butyrate-treated NA cells.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Syncytium formation is induced in the murine neuroblastoma cell cultures which produce pathogenic type G proteins of the rabies virus</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Syncytium formation is induced in the murine neuroblastoma cell cultures which produce pathogenic type G proteins of the rabies virus</title></titleInfo><name type="personal"><namePart type="given">Kinjiro</namePart><namePart type="family">Morimoto</namePart><affiliation>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ya-Jin</namePart><namePart type="family">Ni</namePart><affiliation>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Akihiko</namePart><namePart type="family">Kawai</namePart><affiliation>Department of Molecular Microbiology, Faculty of Pharmaceutical Sciences, Kyoto University, Sakyo-ku 606, Kyoto, Japan</affiliation><description>To whom correspondence and reprint requests should be addressed.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued><copyrightDate encoding="w3cdtf">1992</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: We investigated comparatively the interactions of host cells with two types of rabies virus G protein, an avirulent type G (Gln) and a virulent type G (Arg) protein, having glutamine and arginine at position 333, respectively. For this purpose, we established four types of cell lines (referred to as G(Gln)-NA, G(Arg)-NA, G(Gln)-BHK, and G(Arg)-BHK cells, respectively) by transfecting either the G(Gln)-cDNA or G(Arg)-cDNA into two kinds of cells, murine neuroblastoma C1300 (clone NA) and nonneuronal BHK-21. Both G(Gln)-NA and G(Arg)-NA cells produced G proteins when they were treated with 5 mM sodium butyrate, but only G(Arg)-NA cells formed syncytia at the neutral pH, which was suppressed by anti-G antiserum. The sodium butyrate-treated G(Arg)-NA cells fused also with sodium butyrate-treated NA cells under coculture conditions, but neither with untreated NA cells nor with BHK-21 cells. On the other hand, both G(Gln)-BHK and G(Arg)-BHK cells constitutively produced G proteins, but no syncytium was produced at the neutral pH. G(Arg)-BHK cells, however, formed syncytia with the sodium butyrate-treated NA cells when they were cocultured. These results suggest that only G(Arg) has a potential ability to produce syncytia of NA cells regardless of cell types by which G(Arg) protein was produced and also suggest that a certain cellular factor(s) is required for the syncytium formation, the factor(s) which is lacking in BHK-21 and untreated NA cells but is produced by the sodium butyrate-treated NA cells.</abstract><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1992</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0422-9</identifier><part><date>1992</date><detail type="volume"><number>189</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>405</end></extent><extent unit="pages"><start>203</start><end>216</end></extent></part></relatedItem><identifier type="istex">FDAB8A348A84521FE60ABCA3E3337ED7EDCECB2D</identifier><identifier type="ark">ark:/67375/6H6-PNNGNXG8-X</identifier><identifier type="DOI">10.1016/0042-6822(92)90696-M</identifier><identifier type="PII">0042-6822(92)90696-M</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-PNNGNXG8-X/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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