Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader
Identifieur interne : 000683 ( Istex/Corpus ); précédent : 000682; suivant : 000684Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader
Auteurs : Beate Schwer ; Paolo Visca ; Jan C. Vos ; Hendrik G. StunnenbergSource :
- Cell [ 0092-8674 ] ; 1987.
English descriptors
- Teeft :
- Acad, Binding buffer, Cdna, Dhfr, Discontinuous transcripts, Eukaryotic, Experimental procedures, First strand cdna, Genome, Genomic, Hanggi, Homopolymeric stretches, Hybrid, Late transcripts, Leader sequence, Mapping experiments, Mrna, Natl, Nucleotide, Primer, Primer elongation, Primer extension, Proc, Promoter, Promoter sequences, Readthrough, Readthrough transcripts, Recombinant, Recombinant virus, Rnaase, Rnaase treatment, Sequencing, Size markers, Taaat, Taaat motif, Transcript, Transcriptase, Transcription, Transcription initiation, Translocated, Translocated promoter, Vaccinia, Vaccinia virions, Vaccinia virus.
Abstract
Abstract: We have demonstrated by primer elongation and cap analysis that mature vaccinia virus late transcripts are discontinuously synthesized. We have shown that RNA transcripts from a translocated 11K and from the authentic 11K and 4b late promoters are extended by approximately 35 nucleotides beyond the “start site” determined by S1 mapping using vaccinia genomic DNA as a probe. Sequencing of the RNA and of the first strand cDNA reveal that a homopolymeric poly(A) sequence is linked to the 5′ terminus of the RNA transcripts. S1 mapping of RNA transcripts with a DNA probe containing an A-stretch, replacing promoter sequences upstream of position −1, confirms the existence of a poly(A) leader of approximately 35 A-residues.
Url:
DOI: 10.1016/0092-8674(87)90212-1
Links to Exploration step
ISTEX:E9CD905B90E0DB155F32B782018B4C3BB7217F57Le document en format XML
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S1 mapping of RNA transcripts with a DNA probe containing an A-stretch, replacing promoter sequences upstream of position −1, confirms the existence of a poly(A) leader of approximately 35 A-residues.</abstract><qualityIndicators><refBibsNative>true</refBibsNative><abstractWordCount>115</abstractWordCount><abstractCharCount>736</abstractCharCount><keywordCount>0</keywordCount><score>7.993</score><pdfWordCount>4613</pdfWordCount><pdfCharCount>29556</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>7</pdfPageCount><pdfPageSize>576 x 828 pts</pdfPageSize></qualityIndicators><title>Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader</title><pmid><json:string>3594569</json:string></pmid><pii><json:string>0092-8674(87)90212-1</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Natl. Acad. Sci. 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(1982)</json:string><json:string>Gubler and Hoffmann, 1983</json:string><json:string>Banerjee, 1980</json:string><json:string>Okayama and Berg, 1982</json:string><json:string>Spaan et al., 1982</json:string><json:string>Murphy et al., 1966</json:string><json:string>Maxam and Gilbert (1980)</json:string><json:string>Plucienniczak et al., 1985</json:string><json:string>Hgnggi et al., 1986</json:string><json:string>Venkatesan et al., 1981</json:string><json:string>Baroudy and Moss, 1980</json:string><json:string>Wei and Moss, 1975</json:string><json:string>Beaton and Krug, 1981</json:string><json:string>Yuen and Moss, 1986</json:string><json:string>Mackett et al., 1982</json:string><json:string>Sutton and Boothroyd, 1966</json:string><json:string>Bablanian and Banerjee, 1986</json:string><json:string>Panicali et al., 1982</json:string><json:string>Cochran et al., 1985b</json:string><json:string>Rose1 and Moss, 1985</json:string><json:string>Murphy et al., 1986</json:string><json:string>Urushibara et al., 1975</json:string><json:string>Wei and Moss, 1974</json:string><json:string>Smith et al., 1984</json:string><json:string>Wittek et al., 1984</json:string><json:string>Spaan et al., 1962</json:string><json:string>Bertholet et al., 1985</json:string><json:string>Cochran et al., 1985a</json:string><json:string>Moss, 1976</json:string><json:string>Rose1 and Moss, 1984</json:string><json:string>Spencer et al., 1978</json:string><json:string>Nevins and Joklik, 1975</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-D81LTP1V-V</json:string></ark><categories><wos><json:string>1 - 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We have shown that RNA transcripts from a translocated 11K and from the authentic 11K and 4b late promoters are extended by approximately 35 nucleotides beyond the “start site” determined by S1 mapping using vaccinia genomic DNA as a probe. Sequencing of the RNA and of the first strand cDNA reveal that a homopolymeric poly(A) sequence is linked to the 5′ terminus of the RNA transcripts. S1 mapping of RNA transcripts with a DNA probe containing an A-stretch, replacing promoter sequences upstream of position −1, confirms the existence of a poly(A) leader of approximately 35 A-residues.</p></abstract></profileDesc><revisionDesc><change when="1987-05-05">Modified</change><change when="1987">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-D81LTP1V-V/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>CELL</jid><aid>87902121</aid><ce:pii>0092-8674(87)90212-1</ce:pii><ce:doi>10.1016/0092-8674(87)90212-1</ce:doi><ce:copyright type="unknown" year="1987"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Article</ce:textfn></ce:dochead><ce:title>Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader</ce:title><ce:author-group><ce:author><ce:given-name>Beate</ce:given-name><ce:surname>Schwer</ce:surname></ce:author><ce:author><ce:given-name>Paolo</ce:given-name><ce:surname>Visca</ce:surname></ce:author><ce:author><ce:given-name>Jan C.</ce:given-name><ce:surname>Vos</ce:surname></ce:author><ce:author><ce:given-name>Hendrik G.</ce:given-name><ce:surname>Stunnenberg</ce:surname></ce:author><ce:affiliation><ce:textfn>European Molecular Biology Laboratory Meyerhostraße 1 6900 Heidelberg F.R.G.</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="24" month="3" year="1987"></ce:date-received><ce:date-revised day="5" month="5" year="1987"></ce:date-revised><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>We have demonstrated by primer elongation and cap analysis that mature vaccinia virus late transcripts are discontinuously synthesized. We have shown that RNA transcripts from a translocated 11K and from the authentic 11K and 4b late promoters are extended by approximately 35 nucleotides beyond the “start site” determined by S1 mapping using vaccinia genomic DNA as a probe. Sequencing of the RNA and of the first strand cDNA reveal that a homopolymeric poly(A) sequence is linked to the 5′ terminus of the RNA transcripts. S1 mapping of RNA transcripts with a DNA probe containing an A-stretch, replacing promoter sequences upstream of position −1, confirms the existence of a poly(A) leader of approximately 35 A-residues.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Discontinuous transcription or RNA processing of vaccinia virus late messengers results in a 5′ poly(A) leader</title></titleInfo><name type="personal"><namePart type="given">Beate</namePart><namePart type="family">Schwer</namePart><affiliation>European Molecular Biology Laboratory Meyerhostraße 1 6900 Heidelberg F.R.G.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Paolo</namePart><namePart type="family">Visca</namePart><affiliation>European Molecular Biology Laboratory Meyerhostraße 1 6900 Heidelberg F.R.G.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jan C.</namePart><namePart type="family">Vos</namePart><affiliation>European Molecular Biology Laboratory Meyerhostraße 1 6900 Heidelberg F.R.G.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Hendrik G.</namePart><namePart type="family">Stunnenberg</namePart><affiliation>European Molecular Biology Laboratory Meyerhostraße 1 6900 Heidelberg F.R.G.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1987</dateIssued><dateModified encoding="w3cdtf">1987-05-05</dateModified><copyrightDate encoding="w3cdtf">1987</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: We have demonstrated by primer elongation and cap analysis that mature vaccinia virus late transcripts are discontinuously synthesized. 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S1 mapping of RNA transcripts with a DNA probe containing an A-stretch, replacing promoter sequences upstream of position −1, confirms the existence of a poly(A) leader of approximately 35 A-residues.</abstract><note type="content">Section title: Article</note><relatedItem type="host"><titleInfo><title>Cell</title></titleInfo><titleInfo type="abbreviated"><title>CELL</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1987</dateIssued></originInfo><identifier type="ISSN">0092-8674</identifier><identifier type="PII">S0092-8674(00)X0246-2</identifier><part><date>1987</date><detail type="volume"><number>50</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>147</start><end>325</end></extent><extent unit="pages"><start>163</start><end>169</end></extent></part></relatedItem><identifier type="istex">E9CD905B90E0DB155F32B782018B4C3BB7217F57</identifier><identifier type="ark">ark:/67375/6H6-D81LTP1V-V</identifier><identifier type="DOI">10.1016/0092-8674(87)90212-1</identifier><identifier type="PII">0092-8674(87)90212-1</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-D81LTP1V-V/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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