Growth of canine distemper virus in cultured astrocytes: relationship to in vivo persistence and disease
Identifieur interne : 000345 ( Istex/Corpus ); précédent : 000344; suivant : 000346Growth of canine distemper virus in cultured astrocytes: relationship to in vivo persistence and disease
Auteurs : Susan Pearce-Kelling ; William J. Mitchell ; Brian A. Summers ; Max J. G. AppelSource :
- Microbial Pathogenesis [ 0882-4010 ] ; 1990.
English descriptors
- Teeft :
- Appel, Astrocyte, Astrocyte infection, Astrocyte population, Astrocytic infection, Basal medium, Brain cell cultures, Brain cell passaged, Brain cells, Canine, Canine brain cell cultures, Canine distemper virus, Cell types, Comparative studies, Cornell university, Cultured astrocytes relationship, Days post inoculation, Demyelinating, Distemper, Distemper virus, Encephalitis, Encephalomyelitis, Fresh brain cell cultures, Gfap staining, Gray matter, Harleco corp, Infection, Infectious virus, Inoculation, Lung macrophages, Multinucleated syncytia, Oligodendrocyte, Rapid infection, Rapid rise, Same field, Shorr stain, Subacute, Supernatant, Supernatant fluid, Supernatant fluids, Time point, Vaccinal strain, Veterinary medicine, Viral, Viral antigen, Viral inclusions, Viral strain, Viral strains, Virulent, Virulent strains, Virus strain, Vivo persistence, White matter, White matter disease, York state college.
Abstract
Abstract: Canine distemper virus (CDV) causes an encephalomyelitis in dogs which varies with the viral strain. The CDV Cornell A75-17 strain produces a delayed, subacute to chronic, demyelinating CNS disease. In contrast, the Snyder Hill (CDV-SH) strain-associated neurological disease is more acute in onset, is usually non-demyelinating and primarily produces lesions in the gray matter. In these studies we describe the effects of these two virulent and one avirulent CDV strain, Rockborn (CDV-RO), on astrocytes in dissociated canine brain cell cultures. In multiple replicate experiments, astrocytes were infected most rapidly by CDV-RO [100% of astrocytes were infected by 14 days post-inoculation (pi.)]. This strain caused severe cytopathic effect (CPE) and cytolysis. CDV-SH similarly produced a rapid infection of the astrocytes. In contrast, CDV A75-17 infected less than 25% of the astrocyte population during the first 28 days p.i. (± 7 days); after 28 days p.i., a rapid rise in astrocyte infection occurred. Both virulent viruses caused astrocytic syncytial formation but did not cause cytolysis of the astrocyte population as was observed with the attenuated virus. Titers of infectious virus, released into the supernatant fluid, reflected the degree of astrocyte infection. Virus released by the cultures late in CDV A75-17 infection showed enhanced ability to infect newly derived astrocytes; in contrast, brain cell passaged CDV-SH did not show increased growth in these cells. These results show that (1) there is a difference in growth rate, CPE and capacity for adaptation of three different CDV strains in astrocytes in vitro, and (2) some aspects of the disease (such as persistence in white matter) produced by the virulent strains in vivo may be related to the course of astrocyte infection observed in vitro.
Url:
DOI: 10.1016/0882-4010(90)90009-F
Links to Exploration step
ISTEX:E6C2B365461050E3D0CC47F874BC7560C7B24082Le document en format XML
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Mitchell</name><affiliation><mods:affiliation>J. A. Baker Institute, Department of Microbiology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Summers, Brian A" sort="Summers, Brian A" uniqKey="Summers B" first="Brian A." last="Summers">Brian A. Summers</name><affiliation><mods:affiliation>Department of Pathology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Appel, Max J G" sort="Appel, Max J G" uniqKey="Appel M" first="Max J. G." last="Appel">Max J. G. Appel</name><affiliation><mods:affiliation>J. A. 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The CDV Cornell A75-17 strain produces a delayed, subacute to chronic, demyelinating CNS disease. In contrast, the Snyder Hill (CDV-SH) strain-associated neurological disease is more acute in onset, is usually non-demyelinating and primarily produces lesions in the gray matter. In these studies we describe the effects of these two virulent and one avirulent CDV strain, Rockborn (CDV-RO), on astrocytes in dissociated canine brain cell cultures. In multiple replicate experiments, astrocytes were infected most rapidly by CDV-RO [100% of astrocytes were infected by 14 days post-inoculation (pi.)]. This strain caused severe cytopathic effect (CPE) and cytolysis. CDV-SH similarly produced a rapid infection of the astrocytes. In contrast, CDV A75-17 infected less than 25% of the astrocyte population during the first 28 days p.i. (± 7 days); after 28 days p.i., a rapid rise in astrocyte infection occurred. Both virulent viruses caused astrocytic syncytial formation but did not cause cytolysis of the astrocyte population as was observed with the attenuated virus. Titers of infectious virus, released into the supernatant fluid, reflected the degree of astrocyte infection. Virus released by the cultures late in CDV A75-17 infection showed enhanced ability to infect newly derived astrocytes; in contrast, brain cell passaged CDV-SH did not show increased growth in these cells. These results show that (1) there is a difference in growth rate, CPE and capacity for adaptation of three different CDV strains in astrocytes in vitro, and (2) some aspects of the disease (such as persistence in white matter) produced by the virulent strains in vivo may be related to the course of astrocyte infection observed in vitro.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>astrocyte</json:string><json:string>distemper</json:string><json:string>viral</json:string><json:string>canine</json:string><json:string>astrocyte infection</json:string><json:string>appel</json:string><json:string>demyelinating</json:string><json:string>encephalomyelitis</json:string><json:string>oligodendrocyte</json:string><json:string>subacute</json:string><json:string>canine distemper virus</json:string><json:string>distemper virus</json:string><json:string>brain cell cultures</json:string><json:string>virulent</json:string><json:string>supernatant</json:string><json:string>rapid infection</json:string><json:string>supernatant fluid</json:string><json:string>brain cells</json:string><json:string>canine brain cell cultures</json:string><json:string>shorr stain</json:string><json:string>days post inoculation</json:string><json:string>encephalitis</json:string><json:string>supernatant fluids</json:string><json:string>brain cell passaged</json:string><json:string>viral strains</json:string><json:string>viral antigen</json:string><json:string>infectious virus</json:string><json:string>virulent strains</json:string><json:string>rapid rise</json:string><json:string>virus strain</json:string><json:string>vaccinal strain</json:string><json:string>viral inclusions</json:string><json:string>infection</json:string><json:string>york state college</json:string><json:string>veterinary medicine</json:string><json:string>harleco corp</json:string><json:string>gray matter</json:string><json:string>cornell university</json:string><json:string>time point</json:string><json:string>cultured astrocytes relationship</json:string><json:string>same field</json:string><json:string>basal medium</json:string><json:string>vivo persistence</json:string><json:string>astrocyte population</json:string><json:string>comparative studies</json:string><json:string>viral strain</json:string><json:string>lung macrophages</json:string><json:string>multinucleated syncytia</json:string><json:string>white matter disease</json:string><json:string>gfap staining</json:string><json:string>fresh brain cell cultures</json:string><json:string>astrocytic infection</json:string><json:string>cell types</json:string><json:string>white matter</json:string><json:string>inoculation</json:string></teeft></keywords><author><json:item><name>Susan Pearce-Kelling</name><affiliations><json:string>Department of Pathology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</json:string></affiliations></json:item><json:item><name>William J. Mitchell</name><affiliations><json:string>J. A. Baker Institute, Department of Microbiology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</json:string></affiliations></json:item><json:item><name>Brian A. Summers</name><affiliations><json:string>Department of Pathology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</json:string></affiliations></json:item><json:item><name>Max J.G. Appel</name><affiliations><json:string>J. A. Baker Institute, Department of Microbiology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>canine distemper virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>astrocytes</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>persistent infection</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>encephalomyelitis</value></json:item></subject><arkIstex>ark:/67375/6H6-KJ5G8J41-C</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Canine distemper virus (CDV) causes an encephalomyelitis in dogs which varies with the viral strain. The CDV Cornell A75-17 strain produces a delayed, subacute to chronic, demyelinating CNS disease. In contrast, the Snyder Hill (CDV-SH) strain-associated neurological disease is more acute in onset, is usually non-demyelinating and primarily produces lesions in the gray matter. In these studies we describe the effects of these two virulent and one avirulent CDV strain, Rockborn (CDV-RO), on astrocytes in dissociated canine brain cell cultures. In multiple replicate experiments, astrocytes were infected most rapidly by CDV-RO [100% of astrocytes were infected by 14 days post-inoculation (pi.)]. This strain caused severe cytopathic effect (CPE) and cytolysis. CDV-SH similarly produced a rapid infection of the astrocytes. In contrast, CDV A75-17 infected less than 25% of the astrocyte population during the first 28 days p.i. (± 7 days); after 28 days p.i., a rapid rise in astrocyte infection occurred. Both virulent viruses caused astrocytic syncytial formation but did not cause cytolysis of the astrocyte population as was observed with the attenuated virus. Titers of infectious virus, released into the supernatant fluid, reflected the degree of astrocyte infection. Virus released by the cultures late in CDV A75-17 infection showed enhanced ability to infect newly derived astrocytes; in contrast, brain cell passaged CDV-SH did not show increased growth in these cells. These results show that (1) there is a difference in growth rate, CPE and capacity for adaptation of three different CDV strains in astrocytes in vitro, and (2) some aspects of the disease (such as persistence in white matter) produced by the virulent strains in vivo may be related to the course of astrocyte infection observed in vitro.</abstract><qualityIndicators><score>9.002</score><pdfWordCount>4002</pdfWordCount><pdfCharCount>21579</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>12</pdfPageCount><pdfPageSize>468 x 720 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>280</abstractWordCount><abstractCharCount>1836</abstractCharCount><keywordCount>4</keywordCount></qualityIndicators><title>Growth of canine distemper virus in cultured astrocytes: relationship to in vivo persistence and disease</title><pmid><json:string>2333034</json:string></pmid><pii><json:string>0882-4010(90)90009-F</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Microbial Pathogenesis</title><language><json:string>unknown</json:string></language><publicationDate>1990</publicationDate><issn><json:string>0882-4010</json:string></issn><pii><json:string>S0882-4010(00)X0138-6</json:string></pii><volume>8</volume><issue>1</issue><pages><first>71</first><last>82</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1990</json:string></date><geogName></geogName><orgName><json:string>Baker Institute, Department of Microbiology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York</json:string><json:string>Cornell University</json:string><json:string>Dako Corp</json:string><json:string>Harleco Corp</json:string><json:string>Leitz Corp</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Our</json:string><json:string>Mary Beth</json:string><json:string>Zurbriggen</json:string><json:string>A. Summers</json:string></persName><placeName><json:string>York</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Pearce-Kelling et al.</json:string><json:string>Pearce-Kelling et al</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-KJ5G8J41-C</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - microbiology</json:string><json:string>2 - immunology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - microbiology</json:string></scienceMetrix><scopus><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Infectious Diseases</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Microbiology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string></inist></categories><publicationDate>1990</publicationDate><copyrightDate>1990</copyrightDate><doi><json:string>10.1016/0882-4010(90)90009-F</json:string></doi><id>E6C2B365461050E3D0CC47F874BC7560C7B24082</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-KJ5G8J41-C/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-KJ5G8J41-C/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-KJ5G8J41-C/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Growth of canine distemper virus in cultured astrocytes: relationship to in vivo persistence and disease</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1990</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Growth of canine distemper virus in cultured astrocytes: relationship to in vivo persistence and disease</title><author xml:id="author-0000"><persName><forename type="first">Susan</forename><surname>Pearce-Kelling</surname></persName><affiliation>Department of Pathology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</affiliation></author><author xml:id="author-0001"><persName><forename type="first">William J.</forename><surname>Mitchell</surname></persName><affiliation>J. A. Baker Institute, Department of Microbiology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Brian A.</forename><surname>Summers</surname></persName><affiliation>Department of Pathology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Max J.G.</forename><surname>Appel</surname></persName><affiliation>J. A. Baker Institute, Department of Microbiology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</affiliation></author><idno type="istex">E6C2B365461050E3D0CC47F874BC7560C7B24082</idno><idno type="ark">ark:/67375/6H6-KJ5G8J41-C</idno><idno type="DOI">10.1016/0882-4010(90)90009-F</idno><idno type="PII">0882-4010(90)90009-F</idno></analytic><monogr><title level="j">Microbial Pathogenesis</title><title level="j" type="abbrev">YMPAT</title><idno type="pISSN">0882-4010</idno><idno type="PII">S0882-4010(00)X0138-6</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1990"></date><biblScope unit="volume">8</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="71">71</biblScope><biblScope unit="page" to="82">82</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1990</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Canine distemper virus (CDV) causes an encephalomyelitis in dogs which varies with the viral strain. The CDV Cornell A75-17 strain produces a delayed, subacute to chronic, demyelinating CNS disease. In contrast, the Snyder Hill (CDV-SH) strain-associated neurological disease is more acute in onset, is usually non-demyelinating and primarily produces lesions in the gray matter. In these studies we describe the effects of these two virulent and one avirulent CDV strain, Rockborn (CDV-RO), on astrocytes in dissociated canine brain cell cultures. In multiple replicate experiments, astrocytes were infected most rapidly by CDV-RO [100% of astrocytes were infected by 14 days post-inoculation (pi.)]. This strain caused severe cytopathic effect (CPE) and cytolysis. CDV-SH similarly produced a rapid infection of the astrocytes. In contrast, CDV A75-17 infected less than 25% of the astrocyte population during the first 28 days p.i. (± 7 days); after 28 days p.i., a rapid rise in astrocyte infection occurred. Both virulent viruses caused astrocytic syncytial formation but did not cause cytolysis of the astrocyte population as was observed with the attenuated virus. Titers of infectious virus, released into the supernatant fluid, reflected the degree of astrocyte infection. Virus released by the cultures late in CDV A75-17 infection showed enhanced ability to infect newly derived astrocytes; in contrast, brain cell passaged CDV-SH did not show increased growth in these cells. These results show that (1) there is a difference in growth rate, CPE and capacity for adaptation of three different CDV strains in astrocytes in vitro, and (2) some aspects of the disease (such as persistence in white matter) produced by the virulent strains in vivo may be related to the course of astrocyte infection observed in vitro.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>canine distemper virus</term></item><item><term>astrocytes</term></item><item><term>persistent infection</term></item><item><term>encephalomyelitis</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1990">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-KJ5G8J41-C/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YMPAT</jid><aid>9090009F</aid><ce:pii>0882-4010(90)90009-F</ce:pii><ce:doi>10.1016/0882-4010(90)90009-F</ce:doi><ce:copyright type="unknown" year="1990"></ce:copyright></item-info><head><ce:dochead><ce:textfn>Article</ce:textfn></ce:dochead><ce:title>Growth of canine distemper virus in cultured astrocytes: relationship to <ce:italic>in vivo</ce:italic> persistence and disease</ce:title><ce:author-group><ce:author><ce:given-name>Susan</ce:given-name><ce:surname>Pearce-Kelling</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>William J.</ce:given-name><ce:surname>Mitchell</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Brian A.</ce:given-name><ce:surname>Summers</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Max J.G.</ce:given-name><ce:surname>Appel</ce:surname><ce:cross-ref refid="AFF2"><ce:sup>2</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Department of Pathology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>J. A. Baker Institute, Department of Microbiology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="25" month="8" year="1989"></ce:date-received><ce:date-accepted day="10" month="11" year="1989"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Canine distemper virus (CDV) causes an encephalomyelitis in dogs which varies with the viral strain. The CDV Cornell A75-17 strain produces a delayed, subacute to chronic, demyelinating CNS disease. In contrast, the Snyder Hill (CDV-SH) strain-associated neurological disease is more acute in onset, is usually non-demyelinating and primarily produces lesions in the gray matter. In these studies we describe the effects of these two virulent and one avirulent CDV strain, Rockborn (CDV-RO), on astrocytes in dissociated canine brain cell cultures. In multiple replicate experiments, astrocytes were infected most rapidly by CDV-RO [100% of astrocytes were infected by 14 days post-inoculation (pi.)]. This strain caused severe cytopathic effect (CPE) and cytolysis. CDV-SH similarly produced a rapid infection of the astrocytes. In contrast, CDV A75-17 infected less than 25% of the astrocyte population during the first 28 days p.i. (± 7 days); after 28 days p.i., a rapid rise in astrocyte infection occurred. Both virulent viruses caused astrocytic syncytial formation but did not cause cytolysis of the astrocyte population as was observed with the attenuated virus. Titers of infectious virus, released into the supernatant fluid, reflected the degree of astrocyte infection. Virus released by the cultures late in CDV A75-17 infection showed enhanced ability to infect newly derived astrocytes; in contrast, brain cell passaged CDV-SH did not show increased growth in these cells. These results show that (1) there is a difference in growth rate, CPE and capacity for adaptation of three different CDV strains in astrocytes <ce:italic>in vitro</ce:italic>, and (2) some aspects of the disease (such as persistence in white matter) produced by the virulent strains <ce:italic>in vivo</ce:italic> may be related to the course of astrocyte infection observed <ce:italic>in vitro</ce:italic>.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>canine distemper virus</ce:text></ce:keyword><ce:keyword><ce:text>astrocytes</ce:text></ce:keyword><ce:keyword><ce:text>persistent infection</ce:text></ce:keyword><ce:keyword><ce:text>encephalomyelitis</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Growth of canine distemper virus in cultured astrocytes: relationship to in vivo persistence and disease</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Growth of canine distemper virus in cultured astrocytes: relationship to</title></titleInfo><name type="personal"><namePart type="given">Susan</namePart><namePart type="family">Pearce-Kelling</namePart><affiliation>Department of Pathology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">William J.</namePart><namePart type="family">Mitchell</namePart><affiliation>J. A. Baker Institute, Department of Microbiology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Brian A.</namePart><namePart type="family">Summers</namePart><affiliation>Department of Pathology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Max J.G.</namePart><namePart type="family">Appel</namePart><affiliation>J. A. Baker Institute, Department of Microbiology, New York State College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1990</dateIssued><copyrightDate encoding="w3cdtf">1990</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Canine distemper virus (CDV) causes an encephalomyelitis in dogs which varies with the viral strain. The CDV Cornell A75-17 strain produces a delayed, subacute to chronic, demyelinating CNS disease. In contrast, the Snyder Hill (CDV-SH) strain-associated neurological disease is more acute in onset, is usually non-demyelinating and primarily produces lesions in the gray matter. In these studies we describe the effects of these two virulent and one avirulent CDV strain, Rockborn (CDV-RO), on astrocytes in dissociated canine brain cell cultures. In multiple replicate experiments, astrocytes were infected most rapidly by CDV-RO [100% of astrocytes were infected by 14 days post-inoculation (pi.)]. This strain caused severe cytopathic effect (CPE) and cytolysis. CDV-SH similarly produced a rapid infection of the astrocytes. In contrast, CDV A75-17 infected less than 25% of the astrocyte population during the first 28 days p.i. (± 7 days); after 28 days p.i., a rapid rise in astrocyte infection occurred. Both virulent viruses caused astrocytic syncytial formation but did not cause cytolysis of the astrocyte population as was observed with the attenuated virus. Titers of infectious virus, released into the supernatant fluid, reflected the degree of astrocyte infection. Virus released by the cultures late in CDV A75-17 infection showed enhanced ability to infect newly derived astrocytes; in contrast, brain cell passaged CDV-SH did not show increased growth in these cells. These results show that (1) there is a difference in growth rate, CPE and capacity for adaptation of three different CDV strains in astrocytes in vitro, and (2) some aspects of the disease (such as persistence in white matter) produced by the virulent strains in vivo may be related to the course of astrocyte infection observed in vitro.</abstract><note type="content">Section title: Article</note><subject><genre>Keywords</genre><topic>canine distemper virus</topic><topic>astrocytes</topic><topic>persistent infection</topic><topic>encephalomyelitis</topic></subject><relatedItem type="host"><titleInfo><title>Microbial Pathogenesis</title></titleInfo><titleInfo type="abbreviated"><title>YMPAT</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1990</dateIssued></originInfo><identifier type="ISSN">0882-4010</identifier><identifier type="PII">S0882-4010(00)X0138-6</identifier><part><date>1990</date><detail type="volume"><number>8</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>82</end></extent><extent unit="pages"><start>71</start><end>82</end></extent></part></relatedItem><identifier type="istex">E6C2B365461050E3D0CC47F874BC7560C7B24082</identifier><identifier type="ark">ark:/67375/6H6-KJ5G8J41-C</identifier><identifier type="DOI">10.1016/0882-4010(90)90009-F</identifier><identifier type="PII">0882-4010(90)90009-F</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-KJ5G8J41-C/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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