Intracellular murine hepatitis virus-specific RNAs contain common sequences
Identifieur interne : 000344 ( Istex/Corpus ); précédent : 000343; suivant : 000345Intracellular murine hepatitis virus-specific RNAs contain common sequences
Auteurs : Steve Cheley ; Robert Anderson ; Margaret J. Cupples ; Edwin C. M. Lee Chan ; Vincent L. MorrisSource :
- Virology [ 0042-6822 ] ; 1981.
English descriptors
- Teeft :
- Acad, Actinomycin, Annealing, Aqueous phase, Avian coronavirus, Bronchitis, Bronchitis virus, Cdna, Cdna annealed, Cell monolayers, Cheley, Coronavirus, Coronavirus multiplication strategy, Dalton, Ethanol, Final concentration, Hybridization, Infectious bronchitis virus, Irlfected cells, Major polyadenylated, Molecular weights, Mrna, Murine, Murine coronavirus, Murine hepatitis, Murine hepatitis virus, Nonstructural polypeptides, Nucleic acids, Nucleocapsid protein, Petri dishes, Phosphate buffer, Polyadenylated, Polypeptide, Potassium acetate, Proc, Protein synthesis, Ribonucleic acid, Rna, Room temperature, Semliki forest virus, Several hours, Structural polypeptides, Structural protein, Structural proteins, Subgenomic rnas, Translation products, Translation system, Translational initiation, Tumor virus, Uninfected, Viral, Viral nucleocapsid protein, Viral proteins, Viral rnas, Virol, Wege, Western ontario.
Abstract
Abstract: A major polyadenylated viral RNA of approximately 0.8 × 106 daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct in vitro translation in a cell-free, reticulocyte-derived system. Single stranded 32P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 106 daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences.
Url:
DOI: 10.1016/0042-6822(81)90305-6
Links to Exploration step
ISTEX:5196E0D7B19D5A8D5D3944D754E3189CA2AB90A7Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Intracellular murine hepatitis virus-specific RNAs contain common sequences</title><author><name sortKey="Cheley, Steve" sort="Cheley, Steve" uniqKey="Cheley S" first="Steve" last="Cheley">Steve Cheley</name><affiliation><mods:affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</mods:affiliation></affiliation></author><author><name sortKey="Anderson, Robert" sort="Anderson, Robert" uniqKey="Anderson R" first="Robert" last="Anderson">Robert Anderson</name><affiliation><mods:affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</mods:affiliation></affiliation></author><author><name sortKey="Cupples, Margaret J" sort="Cupples, Margaret J" uniqKey="Cupples M" first="Margaret J." last="Cupples">Margaret J. Cupples</name><affiliation><mods:affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</mods:affiliation></affiliation></author><author><name sortKey="Lee Chan, Edwin C M" sort="Lee Chan, Edwin C M" uniqKey="Lee Chan E" first="Edwin C. M." last="Lee Chan">Edwin C. M. Lee Chan</name><affiliation><mods:affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</mods:affiliation></affiliation></author><author><name sortKey="Morris, Vincent L" sort="Morris, Vincent L" uniqKey="Morris V" first="Vincent L." last="Morris">Vincent L. Morris</name><affiliation><mods:affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:5196E0D7B19D5A8D5D3944D754E3189CA2AB90A7</idno><date when="1981" year="1981">1981</date><idno type="doi">10.1016/0042-6822(81)90305-6</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-T2P1BS2T-5/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000344</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000344</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Intracellular murine hepatitis virus-specific RNAs contain common sequences</title><author><name sortKey="Cheley, Steve" sort="Cheley, Steve" uniqKey="Cheley S" first="Steve" last="Cheley">Steve Cheley</name><affiliation><mods:affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</mods:affiliation></affiliation></author><author><name sortKey="Anderson, Robert" sort="Anderson, Robert" uniqKey="Anderson R" first="Robert" last="Anderson">Robert Anderson</name><affiliation><mods:affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</mods:affiliation></affiliation></author><author><name sortKey="Cupples, Margaret J" sort="Cupples, Margaret J" uniqKey="Cupples M" first="Margaret J." last="Cupples">Margaret J. Cupples</name><affiliation><mods:affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</mods:affiliation></affiliation></author><author><name sortKey="Lee Chan, Edwin C M" sort="Lee Chan, Edwin C M" uniqKey="Lee Chan E" first="Edwin C. M." last="Lee Chan">Edwin C. M. Lee Chan</name><affiliation><mods:affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</mods:affiliation></affiliation></author><author><name sortKey="Morris, Vincent L" sort="Morris, Vincent L" uniqKey="Morris V" first="Vincent L." last="Morris">Vincent L. Morris</name><affiliation><mods:affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1981">1981</date><biblScope unit="volume">112</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="596">596</biblScope><biblScope unit="page" to="604">604</biblScope></imprint><idno type="ISSN">0042-6822</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Acad</term><term>Actinomycin</term><term>Annealing</term><term>Aqueous phase</term><term>Avian coronavirus</term><term>Bronchitis</term><term>Bronchitis virus</term><term>Cdna</term><term>Cdna annealed</term><term>Cell monolayers</term><term>Cheley</term><term>Coronavirus</term><term>Coronavirus multiplication strategy</term><term>Dalton</term><term>Ethanol</term><term>Final concentration</term><term>Hybridization</term><term>Infectious bronchitis virus</term><term>Irlfected cells</term><term>Major polyadenylated</term><term>Molecular weights</term><term>Mrna</term><term>Murine</term><term>Murine coronavirus</term><term>Murine hepatitis</term><term>Murine hepatitis virus</term><term>Nonstructural polypeptides</term><term>Nucleic acids</term><term>Nucleocapsid protein</term><term>Petri dishes</term><term>Phosphate buffer</term><term>Polyadenylated</term><term>Polypeptide</term><term>Potassium acetate</term><term>Proc</term><term>Protein synthesis</term><term>Ribonucleic acid</term><term>Rna</term><term>Room temperature</term><term>Semliki forest virus</term><term>Several hours</term><term>Structural polypeptides</term><term>Structural protein</term><term>Structural proteins</term><term>Subgenomic rnas</term><term>Translation products</term><term>Translation system</term><term>Translational initiation</term><term>Tumor virus</term><term>Uninfected</term><term>Viral</term><term>Viral nucleocapsid protein</term><term>Viral proteins</term><term>Viral rnas</term><term>Virol</term><term>Wege</term><term>Western ontario</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: A major polyadenylated viral RNA of approximately 0.8 × 106 daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct in vitro translation in a cell-free, reticulocyte-derived system. Single stranded 32P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 106 daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>viral</json:string><json:string>rna</json:string><json:string>uninfected</json:string><json:string>polypeptide</json:string><json:string>cdna</json:string><json:string>murine</json:string><json:string>coronavirus</json:string><json:string>virol</json:string><json:string>actinomycin</json:string><json:string>polyadenylated</json:string><json:string>annealing</json:string><json:string>mrna</json:string><json:string>cheley</json:string><json:string>hybridization</json:string><json:string>wege</json:string><json:string>bronchitis</json:string><json:string>acad</json:string><json:string>proc</json:string><json:string>structural proteins</json:string><json:string>molecular weights</json:string><json:string>ethanol</json:string><json:string>major polyadenylated</json:string><json:string>murine hepatitis</json:string><json:string>translation system</json:string><json:string>petri dishes</json:string><json:string>semliki forest virus</json:string><json:string>room temperature</json:string><json:string>structural polypeptides</json:string><json:string>avian coronavirus</json:string><json:string>cell monolayers</json:string><json:string>tumor virus</json:string><json:string>ribonucleic acid</json:string><json:string>several hours</json:string><json:string>protein synthesis</json:string><json:string>aqueous phase</json:string><json:string>nucleocapsid protein</json:string><json:string>murine coronavirus</json:string><json:string>viral nucleocapsid protein</json:string><json:string>cdna annealed</json:string><json:string>translation products</json:string><json:string>viral rnas</json:string><json:string>potassium acetate</json:string><json:string>nonstructural polypeptides</json:string><json:string>translational initiation</json:string><json:string>western ontario</json:string><json:string>murine hepatitis virus</json:string><json:string>structural protein</json:string><json:string>irlfected cells</json:string><json:string>viral proteins</json:string><json:string>nucleic acids</json:string><json:string>infectious bronchitis virus</json:string><json:string>subgenomic rnas</json:string><json:string>phosphate buffer</json:string><json:string>bronchitis virus</json:string><json:string>coronavirus multiplication strategy</json:string><json:string>final concentration</json:string><json:string>dalton</json:string></teeft></keywords><author><json:item><name>Steve Cheley</name><affiliations><json:string>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</json:string></affiliations></json:item><json:item><name>Robert Anderson</name><affiliations><json:string>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</json:string></affiliations></json:item><json:item><name>Margaret J. Cupples</name><affiliations><json:string>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</json:string></affiliations></json:item><json:item><name>Edwin C.M. Lee Chan</name><affiliations><json:string>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</json:string></affiliations></json:item><json:item><name>Vincent L. Morris</name><affiliations><json:string>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-T2P1BS2T-5</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: A major polyadenylated viral RNA of approximately 0.8 × 106 daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct in vitro translation in a cell-free, reticulocyte-derived system. Single stranded 32P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 106 daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences.</abstract><qualityIndicators><refBibsNative>true</refBibsNative><abstractWordCount>123</abstractWordCount><abstractCharCount>862</abstractCharCount><keywordCount>0</keywordCount><score>7.744</score><pdfWordCount>4268</pdfWordCount><pdfCharCount>26887</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>9</pdfPageCount><pdfPageSize>504 x 720 pts</pdfPageSize></qualityIndicators><title>Intracellular murine hepatitis virus-specific RNAs contain common sequences</title><pmid><json:string>6114592</json:string></pmid><pii><json:string>0042-6822(81)90305-6</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>Proc. Nat. Acad. Sci. USA</title><language><json:string>unknown</json:string></language><volume>74</volume><pages><first>5350</first><last>5354</last></pages></serie><host><title>Virology</title><language><json:string>unknown</json:string></language><publicationDate>1981</publicationDate><issn><json:string>0042-6822</json:string></issn><pii><json:string>S0042-6822(00)X0634-4</json:string></pii><volume>112</volume><issue>2</issue><pages><first>596</first><last>604</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1981</json:string><json:string>18S</json:string><json:string>28S</json:string></date><geogName><json:string>Semliki</json:string></geogName><orgName><json:string>Canada Accepted</json:string><json:string>Collaborative Research, Inc</json:string><json:string>National Cancer Institute</json:string><json:string>Office of Program Resources</json:string><json:string>American Type</json:string><json:string>Department of Biochemistry, VIRUS</json:string><json:string>University of Western Ontario</json:string><json:string>Grant MA</json:string><json:string>Grant MA-7321</json:string><json:string>Medical Research Council of Canada</json:string></orgName><orgName_funder><json:string>Grant MA</json:string><json:string>Grant MA-7321</json:string><json:string>Medical Research Council of Canada</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>H. Shida</json:string><json:string>R. Hartwick</json:string><json:string>R. Anderson</json:string><json:string>V. L. Morris</json:string><json:string>Saran Wrap</json:string><json:string>J. Beard</json:string><json:string>Using</json:string></persName><placeName></placeName><ref_url></ref_url><ref_bibl><json:string>McMaster and Carmichael, 1977</json:string><json:string>Glanville et al., 1976</json:string><json:string>Tyrrell et al.</json:string><json:string>Morris et al., 1977</json:string><json:string>Rothfels et al., 1959</json:string><json:string>Jacobson and Baltimore, 1968</json:string><json:string>Hunter et al., 1976</json:string><json:string>Swanstrom and Shank, 1978</json:string><json:string>Bonner and Laskey (1974)</json:string><json:string>Strohman et al. (1977)</json:string><json:string>Wege et al., 1979</json:string><json:string>Bond et al., 1979</json:string><json:string>Rose and Knipe (1975)</json:string><json:string>Mowshowitz, 1973</json:string><json:string>Leong et al., 1972</json:string><json:string>Stern and Kennedy, 1980a, b</json:string><json:string>Aviv and Leder (1972)</json:string><json:string>Alwine et al.</json:string><json:string>Siddell et al. (1980)</json:string><json:string>Lai and Stohlman, 1978</json:string><json:string>Wege et al., 1978</json:string><json:string>Leong et al. (1972)</json:string><json:string>Sturman, 1977</json:string><json:string>Hunter and Gibson, 1978</json:string><json:string>Varmus et al., (1973)</json:string><json:string>Anderson et al., 1979</json:string><json:string>Stern and Kennedy (1980a, b)</json:string><json:string>Watkins et al.</json:string><json:string>Duesberg and Vogt, 1973</json:string><json:string>Laemmli, 1970</json:string><json:string>Pelham and Jackson (1976)</json:string><json:string>Varmus et al. (1973)</json:string><json:string>Shih and Kaesberg, 1973</json:string><json:string>Morris et al. (1977)</json:string><json:string>Shank et al. (1980)</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-T2P1BS2T-5</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - virology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - virology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Virology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string><json:string>4 - biophysique des tissus, organes et organismes</json:string></inist></categories><publicationDate>1981</publicationDate><copyrightDate>1981</copyrightDate><doi><json:string>10.1016/0042-6822(81)90305-6</json:string></doi><id>5196E0D7B19D5A8D5D3944D754E3189CA2AB90A7</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-T2P1BS2T-5/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-T2P1BS2T-5/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-T2P1BS2T-5/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Intracellular murine hepatitis virus-specific RNAs contain common sequences</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1981</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Intracellular murine hepatitis virus-specific RNAs contain common sequences</title><author xml:id="author-0000"><persName><forename type="first">Steve</forename><surname>Cheley</surname></persName><affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Robert</forename><surname>Anderson</surname></persName><affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Margaret J.</forename><surname>Cupples</surname></persName><affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Edwin C.M.</forename><surname>Lee Chan</surname></persName><affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Vincent L.</forename><surname>Morris</surname></persName><note type="biography">To whom reprint requests should be addressed.</note><affiliation>To whom reprint requests should be addressed.</affiliation><affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</affiliation></author><idno type="istex">5196E0D7B19D5A8D5D3944D754E3189CA2AB90A7</idno><idno type="ark">ark:/67375/6H6-T2P1BS2T-5</idno><idno type="DOI">10.1016/0042-6822(81)90305-6</idno><idno type="PII">0042-6822(81)90305-6</idno></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0634-4</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1981"></date><biblScope unit="volume">112</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="596">596</biblScope><biblScope unit="page" to="604">604</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1981</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: A major polyadenylated viral RNA of approximately 0.8 × 106 daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct in vitro translation in a cell-free, reticulocyte-derived system. Single stranded 32P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 106 daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences.</p></abstract></profileDesc><revisionDesc><change when="1981">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-T2P1BS2T-5/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YVIRO</jid><aid>81903056</aid><ce:pii>0042-6822(81)90305-6</ce:pii><ce:doi>10.1016/0042-6822(81)90305-6</ce:doi><ce:copyright type="unknown" year="1981"></ce:copyright></item-info><head><ce:title>Intracellular murine hepatitis virus-specific RNAs contain common sequences</ce:title><ce:author-group><ce:author><ce:given-name>Steve</ce:given-name><ce:surname>Cheley</ce:surname></ce:author><ce:author><ce:given-name>Robert</ce:given-name><ce:surname>Anderson</ce:surname></ce:author><ce:author><ce:given-name>Margaret J.</ce:given-name><ce:surname>Cupples</ce:surname></ce:author><ce:author><ce:given-name>Edwin C.M.</ce:given-name><ce:surname>Lee Chan</ce:surname></ce:author><ce:author><ce:given-name>Vincent L.</ce:given-name><ce:surname>Morris</ce:surname><ce:cross-ref refid="COR1"><ce:sup>1</ce:sup></ce:cross-ref></ce:author><ce:affiliation><ce:textfn>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>1</ce:label><ce:text>To whom reprint requests should be addressed.</ce:text></ce:correspondence></ce:author-group><ce:date-accepted day="12" month="2" year="1981"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>A major polyadenylated viral RNA of approximately 0.8 × 10<ce:sup>6</ce:sup> daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct <ce:italic>in vitro</ce:italic> translation in a cell-free, reticulocyte-derived system. Single stranded <ce:sup>32</ce:sup>P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 10<ce:sup>6</ce:sup> daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Intracellular murine hepatitis virus-specific RNAs contain common sequences</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Intracellular murine hepatitis virus-specific RNAs contain common sequences</title></titleInfo><name type="personal"><namePart type="given">Steve</namePart><namePart type="family">Cheley</namePart><affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Robert</namePart><namePart type="family">Anderson</namePart><affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Margaret J.</namePart><namePart type="family">Cupples</namePart><affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Edwin C.M.</namePart><namePart type="family">Lee Chan</namePart><affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Vincent L.</namePart><namePart type="family">Morris</namePart><affiliation>Department of Microbiology and Immunology, University of Western Ontario, London, Ontario N6A 5C1, Canada</affiliation><description>To whom reprint requests should be addressed.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1981</dateIssued><copyrightDate encoding="w3cdtf">1981</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: A major polyadenylated viral RNA of approximately 0.8 × 106 daltons was isolated from murine hepatitis virus (A59)-infected cells by preparative polyacrylamide gel electrophoresis in formamide. This RNA was shown to encode the viral nucleocapsid protein by direct in vitro translation in a cell-free, reticulocyte-derived system. Single stranded 32P-labeled complementary DNA was prepared from this RNA and was demonstrated to be virus specific. Using this complementary DNA in a Northern blotting procedure, we were able to identify six major virus-specific intracellular RNA species with estimated molecular weights of 0.8, 1.1, 1.4, 1.6, 3, and 4 × 106 daltons. All of these RNA species were polyadenylated. Our results support the idea that coronavirus-infected cells contain multiple intracellular polyadenylated RNAs which share common sequences.</abstract><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1981</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0634-4</identifier><part><date>1981</date><detail type="volume"><number>112</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>375</start><end>822</end></extent><extent unit="pages"><start>596</start><end>604</end></extent></part></relatedItem><identifier type="istex">5196E0D7B19D5A8D5D3944D754E3189CA2AB90A7</identifier><identifier type="ark">ark:/67375/6H6-T2P1BS2T-5</identifier><identifier type="DOI">10.1016/0042-6822(81)90305-6</identifier><identifier type="PII">0042-6822(81)90305-6</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-T2P1BS2T-5/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000344 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000344 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:5196E0D7B19D5A8D5D3944D754E3189CA2AB90A7
|texte= Intracellular murine hepatitis virus-specific RNAs contain common sequences
}}
| This area was generated with Dilib version V0.6.33. |  |