Enrichment of coronavirus-induced interferon-producing blood leukocytes increases the interferon yield per cell: A study with pig leukocytes
Identifieur interne : 000238 ( Istex/Corpus ); précédent : 000237; suivant : 000239Enrichment of coronavirus-induced interferon-producing blood leukocytes increases the interferon yield per cell: A study with pig leukocytes
Auteurs : W. Nowacki ; B. CharleySource :
- Research in Immunology [ 0923-2494 ] ; 1993.
English descriptors
- KwdEn :
- Teeft :
- Alpha interferon, Assay, Blood leukocytes, Brief exposure, Cederblad, Cell depletion, Cell fraction, Cell fractionation, Cell fractionations, Cell fractions, Cell population, Cell supernatants, Cellules, Conical tubes, Constant value, Corona table, Coronavirus, Coronavirus tgev, Dendritic cells, Density gradients, Different cell concentrations, Different experiments, Discontinuous density gradients, Distinct cell population, Elispot, Elispot assay, Ficoll density centrifugation, Filter immunoplaque assay, Final concentration, Foetal calf serum, Gastroenteritis virus, Herpes, Herpes simplex virus, Ifna, Ifna induction, Ifna production, Immunol, Immunomagnetic, Immunomagnetic bead selection, Immunomagnetic selection, Induction, Interferon, Interferon induction, Laude, Lavenant, Leukocyte, Metrizamide, Metrizamide density gradients, Metrizamide gradients, Monoclonal antibodies, Msa3, Pbmc, Percent cells, Percoll, Percoll density gradients, Percoll gradients, Phagocytic cells, Plastic cells, Porcine, Porcine pbmc, Positive selection, Present report, Secrete, Secrete ifna, Sendai virus, Simplex, Supernatant, Tgev, Transmissible gastroenteritis virus, Unfractionated cells, Viable cells, Viral, Viral glycoprotein, Viral induction, Viral infection, Viral infections, Viral structures, Virus induction, Virus particles.
Abstract
Summary: Porcine peripheral blood mononuclear cells, which secrete IFNα in response to a coronavirus, transmissible gastroenteritis virus, were detected by a filter immunoplaque assay (ELISPOT). IFNα-producing cells (IPC), which are present at a low frequency in the blood, could be enriched up to 100-fold by sequential depletion of plastic-adherent cells and cell fractionation on metrizamide density gradients. IPC were present in the non-adherent low-density cell subpopulation. Cell selection experiments using antibody (Ab)-coated immunomagnetic beads revealed that porcine IPC could be positively selected by anti-CD4 or -SLA-class-II Ab, but not by anti-CD2 or -CD8 Ab. The estimated IFN yield per IPC was found to increase when IPC were assayed at higher concentrations. These data suggest that IPC represent a unique and distinct cell population in the blood, which could secrete higher amounts of IFN following its accumulation at a site of viral infection.
fr: L'enrichissement en leucocytes sanguins producteurs d'interféron après induction par un coronavirus accroît la quantité d'interféron produite par celluleLes cellules mononucléées du sang périphérique du porc qui sécrètent l'IFNα après induction par le coronavirus de la gastroentérite transmissible, sont détectables par une technique immunoenzymatique sur filtre (ELISPOT). Ces cellules sont très peu fréquentes dans le sang mais ont pu être enrichies par déplétion des cellules adhérentes au plastique suivie d'une séparation cellulaire sur gradient de métrizamide. Les cellules sécrétrices d'IFNα ont été enrichies dans la fraction de faible densité des cellules non adhérentes. Des expériences de sélection des cellules à l'aide de billes magnétiques recouvertes d'anticorps ont montré que ces cellules étaient positivement sélectionnées par des anticorps anti-CD4 ou anti-SLA-classe-II mais non par des anticorps anti-CD2 ou -CD8. La production estimée d'IFN par cellule sécrétrice a augmenté dans les diverses situations où ces cellules se trouvaient être plus concentrées. Ces résultats suggèrent que les cellules sécrétrices d'IFNα représentent une sous-population cellulaire particulière des cellules sanguines, dont la capacité à produire l'IFN pourrait être accrue du fait de leur accumulation aux sites d'infection virale.
Url:
DOI: 10.1016/0923-2494(93)80066-8
Links to Exploration step
ISTEX:DF6FABE0FB3C0EC34AF0AD7BDD3C1ECE62B5AB06Le document en format XML
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Nowacki</name><affiliation><mods:affiliation>Laboratoire de Virologie et Immunologie moléculaires, INRA, 78350 Jouy-en-Josas (France)</mods:affiliation></affiliation></author><author><name sortKey="Charley, B" sort="Charley, B" uniqKey="Charley B" first="B." last="Charley">B. Charley</name><affiliation><mods:affiliation>Laboratoire de Virologie et Immunologie moléculaires, INRA, 78350 Jouy-en-Josas (France)</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Research in Immunology</title><title level="j" type="abbrev">X83</title><idno type="ISSN">0923-2494</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993">1993</date><biblScope unit="volume">144</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="111">111</biblScope><biblScope unit="page" to="120">120</biblScope></imprint><idno type="ISSN">0923-2494</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0923-2494</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Coronavirus</term><term>ELISPOT</term><term>IPC</term><term>Immunobeads</term><term>Interferon alpha</term><term>Leukocyte</term><term>PBMC</term><term>Transmissible gastroenteritis virus</term><term>mAb</term></keywords><keywords scheme="Teeft" xml:lang="en"><term>Alpha interferon</term><term>Assay</term><term>Blood leukocytes</term><term>Brief exposure</term><term>Cederblad</term><term>Cell depletion</term><term>Cell fraction</term><term>Cell fractionation</term><term>Cell fractionations</term><term>Cell fractions</term><term>Cell population</term><term>Cell supernatants</term><term>Cellules</term><term>Conical tubes</term><term>Constant value</term><term>Corona table</term><term>Coronavirus</term><term>Coronavirus tgev</term><term>Dendritic cells</term><term>Density gradients</term><term>Different cell concentrations</term><term>Different experiments</term><term>Discontinuous density gradients</term><term>Distinct cell population</term><term>Elispot</term><term>Elispot assay</term><term>Ficoll density centrifugation</term><term>Filter immunoplaque assay</term><term>Final concentration</term><term>Foetal calf serum</term><term>Gastroenteritis virus</term><term>Herpes</term><term>Herpes simplex virus</term><term>Ifna</term><term>Ifna induction</term><term>Ifna production</term><term>Immunol</term><term>Immunomagnetic</term><term>Immunomagnetic bead selection</term><term>Immunomagnetic selection</term><term>Induction</term><term>Interferon</term><term>Interferon induction</term><term>Laude</term><term>Lavenant</term><term>Leukocyte</term><term>Metrizamide</term><term>Metrizamide density gradients</term><term>Metrizamide gradients</term><term>Monoclonal antibodies</term><term>Msa3</term><term>Pbmc</term><term>Percent cells</term><term>Percoll</term><term>Percoll density gradients</term><term>Percoll gradients</term><term>Phagocytic cells</term><term>Plastic cells</term><term>Porcine</term><term>Porcine pbmc</term><term>Positive selection</term><term>Present report</term><term>Secrete</term><term>Secrete ifna</term><term>Sendai virus</term><term>Simplex</term><term>Supernatant</term><term>Tgev</term><term>Transmissible gastroenteritis virus</term><term>Unfractionated cells</term><term>Viable cells</term><term>Viral</term><term>Viral glycoprotein</term><term>Viral induction</term><term>Viral infection</term><term>Viral infections</term><term>Viral structures</term><term>Virus induction</term><term>Virus particles</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: Porcine peripheral blood mononuclear cells, which secrete IFNα in response to a coronavirus, transmissible gastroenteritis virus, were detected by a filter immunoplaque assay (ELISPOT). IFNα-producing cells (IPC), which are present at a low frequency in the blood, could be enriched up to 100-fold by sequential depletion of plastic-adherent cells and cell fractionation on metrizamide density gradients. IPC were present in the non-adherent low-density cell subpopulation. Cell selection experiments using antibody (Ab)-coated immunomagnetic beads revealed that porcine IPC could be positively selected by anti-CD4 or -SLA-class-II Ab, but not by anti-CD2 or -CD8 Ab. The estimated IFN yield per IPC was found to increase when IPC were assayed at higher concentrations. These data suggest that IPC represent a unique and distinct cell population in the blood, which could secrete higher amounts of IFN following its accumulation at a site of viral infection.</div><div type="abstract" xml:lang="fr">fr: L'enrichissement en leucocytes sanguins producteurs d'interféron après induction par un coronavirus accroît la quantité d'interféron produite par celluleLes cellules mononucléées du sang périphérique du porc qui sécrètent l'IFNα après induction par le coronavirus de la gastroentérite transmissible, sont détectables par une technique immunoenzymatique sur filtre (ELISPOT). Ces cellules sont très peu fréquentes dans le sang mais ont pu être enrichies par déplétion des cellules adhérentes au plastique suivie d'une séparation cellulaire sur gradient de métrizamide. Les cellules sécrétrices d'IFNα ont été enrichies dans la fraction de faible densité des cellules non adhérentes. Des expériences de sélection des cellules à l'aide de billes magnétiques recouvertes d'anticorps ont montré que ces cellules étaient positivement sélectionnées par des anticorps anti-CD4 ou anti-SLA-classe-II mais non par des anticorps anti-CD2 ou -CD8. La production estimée d'IFN par cellule sécrétrice a augmenté dans les diverses situations où ces cellules se trouvaient être plus concentrées. Ces résultats suggèrent que les cellules sécrétrices d'IFNα représentent une sous-population cellulaire particulière des cellules sanguines, dont la capacité à produire l'IFN pourrait être accrue du fait de leur accumulation aux sites d'infection virale.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>ifna</json:string><json:string>pbmc</json:string><json:string>metrizamide</json:string><json:string>interferon</json:string><json:string>leukocyte</json:string><json:string>elispot</json:string><json:string>viral</json:string><json:string>porcine</json:string><json:string>immunomagnetic</json:string><json:string>percoll</json:string><json:string>cellules</json:string><json:string>tgev</json:string><json:string>coronavirus</json:string><json:string>virus induction</json:string><json:string>assay</json:string><json:string>elispot assay</json:string><json:string>herpes</json:string><json:string>immunol</json:string><json:string>metrizamide gradients</json:string><json:string>cederblad</json:string><json:string>lavenant</json:string><json:string>msa3</json:string><json:string>secrete</json:string><json:string>laude</json:string><json:string>supernatant</json:string><json:string>dendritic cells</json:string><json:string>herpes simplex virus</json:string><json:string>simplex</json:string><json:string>discontinuous density gradients</json:string><json:string>phagocytic cells</json:string><json:string>porcine pbmc</json:string><json:string>cell fractions</json:string><json:string>viral structures</json:string><json:string>percent cells</json:string><json:string>unfractionated cells</json:string><json:string>cell fractionations</json:string><json:string>coronavirus tgev</json:string><json:string>sendai virus</json:string><json:string>cell population</json:string><json:string>alpha interferon</json:string><json:string>induction</json:string><json:string>foetal calf serum</json:string><json:string>transmissible gastroenteritis virus</json:string><json:string>gastroenteritis virus</json:string><json:string>viral infection</json:string><json:string>cell fractionation</json:string><json:string>secrete ifna</json:string><json:string>viral glycoprotein</json:string><json:string>percoll density gradients</json:string><json:string>conical tubes</json:string><json:string>plastic cells</json:string><json:string>cell fraction</json:string><json:string>present report</json:string><json:string>viable cells</json:string><json:string>final concentration</json:string><json:string>viral induction</json:string><json:string>cell depletion</json:string><json:string>ifna production</json:string><json:string>corona table</json:string><json:string>immunomagnetic selection</json:string><json:string>brief exposure</json:string><json:string>percoll gradients</json:string><json:string>positive selection</json:string><json:string>metrizamide density gradients</json:string><json:string>cell supernatants</json:string><json:string>blood leukocytes</json:string><json:string>virus particles</json:string><json:string>different cell concentrations</json:string><json:string>constant value</json:string><json:string>different experiments</json:string><json:string>filter immunoplaque assay</json:string><json:string>viral infections</json:string><json:string>ifna induction</json:string><json:string>immunomagnetic bead selection</json:string><json:string>distinct cell population</json:string><json:string>monoclonal antibodies</json:string><json:string>ficoll density centrifugation</json:string><json:string>interferon induction</json:string><json:string>density gradients</json:string></teeft></keywords><subject><json:item><lang><json:string>eng</json:string></lang><value>Coronavirus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Leukocyte</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Interferon alpha</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Transmissible gastroenteritis virus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>IPC</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ELISPOT</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Immunobeads</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PBMC</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>mAb</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Leucocyte</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Coronavirus</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Interféron alpha</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Virus de la gastroentérite transmissible</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Cellules productrices d'interféron</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>ELISPOT</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Billes immunomagnétiques</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PBMC</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Anticorps monoclonaux</value></json:item></subject><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><qualityIndicators><score>8.024</score><pdfWordCount>4320</pdfWordCount><pdfCharCount>30499</pdfCharCount><pdfVersion>1.3</pdfVersion><pdfPageCount>10</pdfPageCount><pdfPageSize>612 x 792 pts (letter)</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>142</abstractWordCount><abstractCharCount>968</abstractCharCount><keywordCount>18</keywordCount></qualityIndicators><title>Enrichment of coronavirus-induced interferon-producing blood leukocytes increases the interferon yield per cell: A study with pig leukocytes</title><pii><json:string>0923-2494(93)80066-8</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Research in Immunology</title><language><json:string>unknown</json:string></language><publicationDate>1993</publicationDate><issn><json:string>0923-2494</json:string></issn><pii><json:string>S0923-2494(00)X0032-6</json:string></pii><volume>144</volume><issue>2</issue><pages><first>111</first><last>120</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1993</json:string></date><geogName></geogName><orgName></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>B. Charley</json:string><json:string>F. Hervatin</json:string><json:string>Vo Percoll</json:string><json:string>C. de Vaureix</json:string><json:string>I. Effect</json:string><json:string>The</json:string><json:string>J. Lunney</json:string><json:string>U. Koszinowski</json:string><json:string>W. Nowacki</json:string><json:string>A.M. Spite</json:string></persName><placeName><json:string>Uppsala</json:string><json:string>Specificity</json:string><json:string>France</json:string><json:string>Sweden</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Cederblad and Alm, 1991</json:string><json:string>Ito et al., 1981</json:string><json:string>Capobianchi et al., 1985</json:string><json:string>Lefevre et al., 1990</json:string><json:string>Chehimi et al., 1989</json:string><json:string>Cederblad and Alm, 1990</json:string><json:string>Charley and Laude, 1988</json:string><json:string>reviewed in Charley and Laude, 1992</json:string><json:string>FitzgeraldBocarsly et al., 1988</json:string><json:string>La Bonnardiere and Laude, 1981</json:string><json:string>Lunney and Pescovitz, 1987</json:string><json:string>Feldman and Fitzgerald-Bocarsly , 1990</json:string><json:string>Knight et al. (1986)</json:string><json:string>Ito et al., 1978</json:string><json:string>Charley and Lavenant, 1990</json:string><json:string>Charley et al., 1991</json:string><json:string>Sandberg et al., 1990</json:string><json:string>Laude et al., 1992</json:string><json:string>Lebon et al., 1982</json:string><json:string>Lefevre et al. (1990)</json:string><json:string>Haridon et al. (1991)</json:string><json:string>Feldman and FitzgeraldBocarsly, 1990</json:string><json:string>Sandberg et al., 1989</json:string><json:string>Kurane et al., 1986</json:string><json:string>Feldman and Fitzgerald-Bocarsly, 1990</json:string><json:string>Lebon, 1985</json:string><json:string>Fitzgerald-Bocarsly et al. (1988)</json:string><json:string>Capobianchi et al., 1992</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-5V4VS29N-K</json:string></ark><categories><wos></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - clinical medicine</json:string><json:string>3 - immunology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Immunology and Microbiology</json:string><json:string>3 - Immunology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string></inist></categories><publicationDate>1993</publicationDate><author><json:item><name>W. Nowacki</name><affiliations><json:string>Laboratoire de Virologie et Immunologie moléculaires, INRA, 78350 Jouy-en-Josas (France)</json:string></affiliations></json:item><json:item><name>B. Charley</name><affiliations><json:string>Laboratoire de Virologie et Immunologie moléculaires, INRA, 78350 Jouy-en-Josas (France)</json:string></affiliations></json:item></author><articleId><json:string>93800668</json:string></articleId><arkIstex>ark:/67375/6H6-5V4VS29N-K</arkIstex><abstract>Summary: Porcine peripheral blood mononuclear cells, which secrete IFNα in response to a coronavirus, transmissible gastroenteritis virus, were detected by a filter immunoplaque assay (ELISPOT). IFNα-producing cells (IPC), which are present at a low frequency in the blood, could be enriched up to 100-fold by sequential depletion of plastic-adherent cells and cell fractionation on metrizamide density gradients. IPC were present in the non-adherent low-density cell subpopulation. Cell selection experiments using antibody (Ab)-coated immunomagnetic beads revealed that porcine IPC could be positively selected by anti-CD4 or -SLA-class-II Ab, but not by anti-CD2 or -CD8 Ab. The estimated IFN yield per IPC was found to increase when IPC were assayed at higher concentrations. These data suggest that IPC represent a unique and distinct cell population in the blood, which could secrete higher amounts of IFN following its accumulation at a site of viral infection.</abstract><pmid><json:string>8390709</json:string></pmid><serie><title>Proc. Soc. Exp. Biol. Med.</title><language><json:string>unknown</json:string></language><volume>178</volume><pages><first>551</first><last>556</last></pages></serie><copyrightDate>1993</copyrightDate><doi><json:string>10.1016/0923-2494(93)80066-8</json:string></doi><id>DF6FABE0FB3C0EC34AF0AD7BDD3C1ECE62B5AB06</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5V4VS29N-K/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5V4VS29N-K/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-5V4VS29N-K/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a" type="main" xml:lang="en">Enrichment of coronavirus-induced interferon-producing blood leukocytes increases the interferon yield per cell: A study with pig leukocytes</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>©1993 Institut Pasteur/Elsevier Paris</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</p></availability><date>1993</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Original article</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Enrichment of coronavirus-induced interferon-producing blood leukocytes increases the interferon yield per cell: A study with pig leukocytes</title><author xml:id="author-0000"><persName><forename type="first">W.</forename><surname>Nowacki</surname></persName><affiliation>Laboratoire de Virologie et Immunologie moléculaires, INRA, 78350 Jouy-en-Josas (France)</affiliation></author><author xml:id="author-0001"><persName><forename type="first">B.</forename><surname>Charley</surname></persName><note type="correspondence"><p>Corresponding author.</p></note><affiliation>Laboratoire de Virologie et Immunologie moléculaires, INRA, 78350 Jouy-en-Josas (France)</affiliation></author><idno type="istex">DF6FABE0FB3C0EC34AF0AD7BDD3C1ECE62B5AB06</idno><idno type="ark">ark:/67375/6H6-5V4VS29N-K</idno><idno type="DOI">10.1016/0923-2494(93)80066-8</idno><idno type="PII">0923-2494(93)80066-8</idno><idno type="article-id">93800668</idno></analytic><monogr><title level="j">Research in Immunology</title><title level="j" type="abbrev">X83</title><idno type="pISSN">0923-2494</idno><idno type="PII">S0923-2494(00)X0032-6</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1993"></date><biblScope unit="volume">144</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="111">111</biblScope><biblScope unit="page" to="120">120</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1993</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Summary: Porcine peripheral blood mononuclear cells, which secrete IFNα in response to a coronavirus, transmissible gastroenteritis virus, were detected by a filter immunoplaque assay (ELISPOT). IFNα-producing cells (IPC), which are present at a low frequency in the blood, could be enriched up to 100-fold by sequential depletion of plastic-adherent cells and cell fractionation on metrizamide density gradients. IPC were present in the non-adherent low-density cell subpopulation. Cell selection experiments using antibody (Ab)-coated immunomagnetic beads revealed that porcine IPC could be positively selected by anti-CD4 or -SLA-class-II Ab, but not by anti-CD2 or -CD8 Ab. The estimated IFN yield per IPC was found to increase when IPC were assayed at higher concentrations. These data suggest that IPC represent a unique and distinct cell population in the blood, which could secrete higher amounts of IFN following its accumulation at a site of viral infection.</p></abstract><abstract xml:lang="fr"><p>fr: L'enrichissement en leucocytes sanguins producteurs d'interféron après induction par un coronavirus accroît la quantité d'interféron produite par celluleLes cellules mononucléées du sang périphérique du porc qui sécrètent l'IFNα après induction par le coronavirus de la gastroentérite transmissible, sont détectables par une technique immunoenzymatique sur filtre (ELISPOT). Ces cellules sont très peu fréquentes dans le sang mais ont pu être enrichies par déplétion des cellules adhérentes au plastique suivie d'une séparation cellulaire sur gradient de métrizamide. Les cellules sécrétrices d'IFNα ont été enrichies dans la fraction de faible densité des cellules non adhérentes. Des expériences de sélection des cellules à l'aide de billes magnétiques recouvertes d'anticorps ont montré que ces cellules étaient positivement sélectionnées par des anticorps anti-CD4 ou anti-SLA-classe-II mais non par des anticorps anti-CD2 ou -CD8. La production estimée d'IFN par cellule sécrétrice a augmenté dans les diverses situations où ces cellules se trouvaient être plus concentrées. Ces résultats suggèrent que les cellules sécrétrices d'IFNα représentent une sous-population cellulaire particulière des cellules sanguines, dont la capacité à produire l'IFN pourrait être accrue du fait de leur accumulation aux sites d'infection virale.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><item><term>Coronavirus</term></item><item><term>Leukocyte</term></item><item><term>Interferon alpha</term></item><item><term>Transmissible gastroenteritis virus</term></item><item><term>IPC</term></item><item><term>ELISPOT</term></item><item><term>Immunobeads</term></item><item><term>PBMC</term></item><item><term>mAb</term></item></list></keywords></textClass><textClass xml:lang="fr"><keywords scheme="keyword"><list><head>Motsclés</head><item><term>Leucocyte</term></item><item><term>Coronavirus</term></item><item><term>Interféron alpha</term></item><item><term>Virus de la gastroentérite transmissible</term></item><item><term>Cellules productrices d'interféron</term></item><item><term>ELISPOT</term></item><item><term>Billes immunomagnétiques</term></item><item><term>PBMC</term></item><item><term>Anticorps monoclonaux</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1993">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5V4VS29N-K/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier doc found" wicri:toSee="Elsevier, no converted or simple article"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 5.0.1//EN//XML" URI="art501.dtd" name="istex:docType"></istex:docType><istex:document><article version="5.0" xml:lang="en" docsubtype="fla"><item-info><jid>X83</jid><aid>93800668</aid><ce:pii>0923-2494(93)80066-8</ce:pii><ce:doi>10.1016/0923-2494(93)80066-8</ce:doi><ce:copyright type="unknown" year="1993">Institut Pasteur/Elsevier Paris</ce:copyright></item-info><head><ce:dochead><ce:textfn>Original article</ce:textfn></ce:dochead><ce:title>Enrichment of coronavirus-induced interferon-producing blood leukocytes increases the interferon yield per cell: A study with pig leukocytes</ce:title><ce:author-group><ce:author><ce:given-name>W.</ce:given-name><ce:surname>Nowacki</ce:surname></ce:author><ce:author><ce:given-name>B.</ce:given-name><ce:surname>Charley</ce:surname><ce:cross-ref refid="cor1"><ce:sup>*</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="aff1"><ce:textfn>Laboratoire de Virologie et Immunologie moléculaires, INRA, 78350 Jouy-en-Josas (France)</ce:textfn></ce:affiliation><ce:correspondence id="cor1"><ce:label>*</ce:label><ce:text>Corresponding author.</ce:text></ce:correspondence></ce:author-group><ce:date-received day="12" month="12" year="1992"></ce:date-received><ce:date-accepted day="19" month="1" year="1993"></ce:date-accepted><ce:abstract id="ab1" class="author" xml:lang="en"><ce:section-title>Summary</ce:section-title><ce:abstract-sec><ce:simple-para>Porcine peripheral blood mononuclear cells, which secrete IFNα in response to a coronavirus, transmissible gastroenteritis virus, were detected by a filter immunoplaque assay (ELISPOT). IFNα-producing cells (IPC), which are present at a low frequency in the blood, could be enriched up to 100-fold by sequential depletion of plastic-adherent cells and cell fractionation on metrizamide density gradients. IPC were present in the non-adherent low-density cell subpopulation. Cell selection experiments using antibody (Ab)-coated immunomagnetic beads revealed that porcine IPC could be positively selected by anti-CD4 or -SLA-class-II Ab, but not by anti-CD2 or -CD8 Ab. The estimated IFN yield per IPC was found to increase when IPC were assayed at higher concentrations. These data suggest that IPC represent a unique and distinct cell population in the blood, which could secrete higher amounts of IFN following its accumulation at a site of viral infection.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:abstract id="ab2" class="author" xml:lang="fr"><ce:section-title>fr</ce:section-title><ce:abstract-sec><ce:simple-para>L'enrichissement en leucocytes sanguins producteurs d'interféron après induction par un coronavirus accroît la quantité d'interféron produite par cellule</ce:simple-para><ce:simple-para>Les cellules mononucléées du sang périphérique du porc qui sécrètent l'IFNα après induction par le coronavirus de la gastroentérite transmissible, sont détectables par une technique immunoenzymatique sur filtre (ELISPOT). Ces cellules sont très peu fréquentes dans le sang mais ont pu être enrichies par déplétion des cellules adhérentes au plastique suivie d'une séparation cellulaire sur gradient de métrizamide. Les cellules sécrétrices d'IFNα ont été enrichies dans la fraction de faible densité des cellules non adhérentes. Des expériences de sélection des cellules à l'aide de billes magnétiques recouvertes d'anticorps ont montré que ces cellules étaient positivement sélectionnées par des anticorps anti-CD4 ou anti-SLA-classe-II mais non par des anticorps anti-CD2 ou -CD8. La production estimée d'IFN par cellule sécrétrice a augmenté dans les diverses situations où ces cellules se trouvaient être plus concentrées. Ces résultats suggèrent que les cellules sécrétrices d'IFNα représentent une sous-population cellulaire particulière des cellules sanguines, dont la capacité à produire l'IFN pourrait être accrue du fait de leur accumulation aux sites d'infection virale.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords xml:lang="en"><ce:keyword><ce:text>Coronavirus</ce:text></ce:keyword><ce:keyword><ce:text>Leukocyte</ce:text></ce:keyword><ce:keyword><ce:text>Interferon alpha</ce:text></ce:keyword><ce:keyword><ce:text>Transmissible gastroenteritis virus</ce:text></ce:keyword><ce:keyword><ce:text>IPC</ce:text></ce:keyword><ce:keyword><ce:text>ELISPOT</ce:text></ce:keyword><ce:keyword><ce:text>Immunobeads</ce:text></ce:keyword><ce:keyword><ce:text>PBMC</ce:text></ce:keyword><ce:keyword><ce:text>mAb</ce:text></ce:keyword></ce:keywords><ce:keywords xml:lang="fr"><ce:section-title>Motsclés</ce:section-title><ce:keyword><ce:text>Leucocyte</ce:text></ce:keyword><ce:keyword><ce:text>Coronavirus</ce:text></ce:keyword><ce:keyword><ce:text>Interféron alpha</ce:text></ce:keyword><ce:keyword><ce:text>Virus de la gastroentérite transmissible</ce:text></ce:keyword><ce:keyword><ce:text>Cellules productrices d'interféron</ce:text></ce:keyword><ce:keyword><ce:text>ELISPOT</ce:text></ce:keyword><ce:keyword><ce:text>Billes immunomagnétiques</ce:text></ce:keyword><ce:keyword><ce:text>PBMC</ce:text></ce:keyword><ce:keyword><ce:text>Anticorps monoclonaux</ce:text></ce:keyword></ce:keywords></head><tail><ce:bibliography><ce:section-title>References</ce:section-title><ce:bibliography-sec><ce:bib-reference id="bib1"><ce:label>Capobianchi, et al, 1985</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Capobianchi</ce:surname><ce:given-name>M.R.</ce:given-name></sb:author><sb:author><ce:surname>Facchini</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>Di Marco</ce:surname><ce:given-name>P.</ce:given-name></sb:author><sb:author><ce:surname>Antonelli</ce:surname><ce:given-name>G.</ce:given-name></sb:author><sb:author><ce:surname>Dianzani</ce:surname><ce:given-name>F.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Induction of alpha interferon by membrane interaction between viral surface and peripheral blood mononuclear cells</sb:maintitle></sb:title></sb:contribution><sb:host><sb:edited-book><sb:book-series><sb:series><sb:title><sb:maintitle>Proc. Soc. Exp. Biol. Med.</sb:maintitle></sb:title><sb:volume-nr>178</sb:volume-nr></sb:series></sb:book-series><sb:date>1985</sb:date></sb:edited-book><sb:pages><sb:first-page>551</sb:first-page><sb:last-page>556</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib2"><ce:label>Capobianchi, et al, 1992</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Capobianchi</ce:surname><ce:given-name>M.R.</ce:given-name></sb:author><sb:author><ce:surname>Ankel</ce:surname><ce:given-name>H.</ce:given-name></sb:author><sb:author><ce:surname>Ameglio</ce:surname><ce:given-name>F.</ce:given-name></sb:author><sb:author><ce:surname>Paganelli</ce:surname><ce:given-name>R.</ce:given-name></sb:author><sb:author><ce:surname>Pizzoli</ce:surname><ce:given-name>P.</ce:given-name></sb:author><sb:author><ce:surname>Dianzani</ce:surname><ce:given-name>F.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Recombinant glycoprotein 120 of human immunodeficiency virus is a potent interferon inducer</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>AIDS Res. Hum. Retroviruses</sb:maintitle></sb:title><sb:volume-nr>8</sb:volume-nr></sb:series><sb:date>1992</sb:date></sb:issue><sb:pages><sb:first-page>575</sb:first-page><sb:last-page>579</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib3"><ce:label>Cederblad and Alm, 1990</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Cederblad</ce:surname><ce:given-name>B.</ce:given-name></sb:author><sb:author><ce:surname>Alm</ce:surname><ce:given-name>G.V.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Infrequent but efficient interferon-α-producing human mononuclear leukocytes induced by herpes simplex virus <ce:italic>in vitro</ce:italic> studied by immuno-plaque and limiting dilution assays</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Interferon Res.</sb:maintitle></sb:title><sb:volume-nr>10</sb:volume-nr></sb:series><sb:date>1990</sb:date></sb:issue><sb:pages><sb:first-page>65</sb:first-page><sb:last-page>73</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib4"><ce:label>Cederblad and Alm, 1991</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Cederblad</ce:surname><ce:given-name>B.</ce:given-name></sb:author><sb:author><ce:surname>Alm</ce:surname><ce:given-name>G.V.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Interferons and the colony-stimulating factors IL-3 and GM-CSF enhance the IFN-α response in human blood leucocytes induced by herpes simplex virus</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Scand. J. Immunol.</sb:maintitle></sb:title><sb:volume-nr>34</sb:volume-nr></sb:series><sb:date>1991</sb:date></sb:issue><sb:pages><sb:first-page>549</sb:first-page><sb:last-page>555</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib5"><ce:label>Charley and Laude, 1988</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Charley</ce:surname><ce:given-name>B.</ce:given-name></sb:author><sb:author><ce:surname>Laude</ce:surname><ce:given-name>H.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Induction of alpha interferon by transmissible gastroenteritis coronavirus: role of transmembrane glycoprotein E1</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Virol.</sb:maintitle></sb:title><sb:volume-nr>62</sb:volume-nr></sb:series><sb:date>1988</sb:date></sb:issue><sb:pages><sb:first-page>8</sb:first-page><sb:last-page>11</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib6"><ce:label>Charley and Lavenant, 1990</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Charley</ce:surname><ce:given-name>B.</ce:given-name></sb:author><sb:author><ce:surname>Lavenant</ce:surname><ce:given-name>L.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Characterization of blood mononuclear cells producting IFNα following induction by coronavirus-infected cells (porcine transmissible gastroenteritis virus)</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Res. Immunol.</sb:maintitle></sb:title><sb:volume-nr>141</sb:volume-nr></sb:series><sb:date>1990</sb:date></sb:issue><sb:pages><sb:first-page>141</sb:first-page><sb:last-page>151</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib7"><ce:label>Charley, et al, 1991</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Charley</ce:surname><ce:given-name>B.</ce:given-name></sb:author><sb:author><ce:surname>Lavenant</ce:surname><ce:given-name>L.</ce:given-name></sb:author><sb:author><ce:surname>Delmas</ce:surname><ce:given-name>B.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Glycosylation is required for coronavirus TGEV to induce an efficient production of IFNα by blood mononuclear cells</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Scand. J. Immunol.</sb:maintitle></sb:title><sb:volume-nr>33</sb:volume-nr></sb:series><sb:date>1991</sb:date></sb:issue><sb:pages><sb:first-page>435</sb:first-page><sb:last-page>440</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib8"><ce:label>Charley and Laude, 1992</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Charley</ce:surname><ce:given-name>B.</ce:given-name></sb:author><sb:author><ce:surname>Laude</ce:surname><ce:given-name>H.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Interactions viruslymphocytes pour la production d'interféron-α</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Ann. Rech. Vet.</sb:maintitle></sb:title><sb:volume-nr>23</sb:volume-nr></sb:series><sb:date>1992</sb:date></sb:issue><sb:pages><sb:first-page>318</sb:first-page><sb:last-page>322</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib9"><ce:label>Chehimi, et al, 1989</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Chehimi</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>Starr</ce:surname><ce:given-name>S.E.</ce:given-name></sb:author><sb:author><ce:surname>Kawashima</ce:surname><ce:given-name>H.</ce:given-name></sb:author><sb:author><ce:surname>Miller</ce:surname><ce:given-name>D.S.</ce:given-name></sb:author><sb:author><ce:surname>Trinchieri</ce:surname><ce:given-name>G.</ce:given-name></sb:author><sb:author><ce:surname>Perussia</ce:surname><ce:given-name>B.</ce:given-name></sb:author><sb:author><ce:surname>Bandyopadhyay</ce:surname><ce:given-name>S.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Dendritic cells and IFN-α-producing cells are two functionally distinct non-B, non-monocytic HLADR<ce:sup loc="post">+</ce:sup> cell subsets in human peripheral blood</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Immunology</sb:maintitle></sb:title><sb:volume-nr>68</sb:volume-nr></sb:series><sb:date>1989</sb:date></sb:issue><sb:pages><sb:first-page>486</sb:first-page><sb:last-page>490</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib10"><ce:label>Feldman and Fitzgerald-Bocarsly, 1990</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Feldman</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>Fitzgerald-Bocarsly</ce:surname><ce:given-name>P.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Sequential enrichment and immunocytochemical visualization of human interferon-α-producing cells</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Interferon Res.</sb:maintitle></sb:title><sb:volume-nr>10</sb:volume-nr></sb:series><sb:date>1990</sb:date></sb:issue><sb:pages><sb:first-page>435</sb:first-page><sb:last-page>446</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib11"><ce:label>Fitzgerald-Bocarsly, et al, 1988</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Fitzgerald-Bocarsly</ce:surname><ce:given-name>P.</ce:given-name></sb:author><sb:author><ce:surname>Feldman</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>Mendelsohn</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>Curl</ce:surname><ce:given-name>S.</ce:given-name></sb:author><sb:author><ce:surname>Lopez</ce:surname><ce:given-name>C.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Human mononuclear cells which produce interferon-alpha during NK (HSV-FS) assays are HLA-DR-positive cells distinct from cytolytic natural killer effectors</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Leukocyte Biol.</sb:maintitle></sb:title><sb:volume-nr>43</sb:volume-nr></sb:series><sb:date>1988</sb:date></sb:issue><sb:pages><sb:first-page>323</sb:first-page><sb:last-page>334</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib12"><ce:label>Gobl, et al, 1988</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Gobl</ce:surname><ce:given-name>A.E.</ce:given-name></sb:author><sb:author><ce:surname>Funa</ce:surname><ce:given-name>K.</ce:given-name></sb:author><sb:author><ce:surname>Alm</ce:surname><ce:given-name>G.V.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Different induction patterns of mRNA for IFN-α and -β in human mononuclear leukocytes after <ce:italic>in vitro</ce:italic> stimulation with herpes simplex virus-infected fibroblasts and Sendai virus</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Immunol.</sb:maintitle></sb:title><sb:volume-nr>140</sb:volume-nr></sb:series><sb:date>1988</sb:date></sb:issue><sb:pages><sb:first-page>3605</sb:first-page><sb:last-page>3609</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib13"><ce:label>Ito, et al, 1981</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Ito</ce:surname><ce:given-name>Y.</ce:given-name></sb:author><sb:author><ce:surname>Aoki</ce:surname><ce:given-name>H.</ce:given-name></sb:author><sb:author><ce:surname>Kimura</ce:surname><ce:given-name>Y.</ce:given-name></sb:author><sb:author><ce:surname>Takano</ce:surname><ce:given-name>M.</ce:given-name></sb:author><sb:author><ce:surname>Shimokata</ce:surname><ce:given-name>K.</ce:given-name></sb:author><sb:author><ce:surname>Maeno</ce:surname><ce:given-name>K.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Natural interferon-producing cells in mice</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Infect. Immun.</sb:maintitle></sb:title><sb:volume-nr>31</sb:volume-nr></sb:series><sb:date>1981</sb:date></sb:issue><sb:pages><sb:first-page>519</sb:first-page><sb:last-page>523</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib14"><ce:label>Ito, et al, 1978</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Ito</ce:surname><ce:given-name>Y.</ce:given-name></sb:author><sb:author><ce:surname>Nishiyama</ce:surname><ce:given-name>Y.</ce:given-name></sb:author><sb:author><ce:surname>Shimokata</ce:surname><ce:given-name>K.</ce:given-name></sb:author><sb:author><ce:surname>Takeyama</ce:surname><ce:given-name>H.</ce:given-name></sb:author><sb:author><ce:surname>Kunii</ce:surname><ce:given-name>A.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Active component of HVJ (Sendai virus) for interferon induction in mice</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle><ce:italic>Nature</ce:italic> (Lond.)</sb:maintitle></sb:title><sb:volume-nr>274</sb:volume-nr></sb:series><sb:date>1978</sb:date></sb:issue><sb:pages><sb:first-page>801</sb:first-page><sb:last-page>802</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib15"><ce:label>Knight, et al, 1986</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Knight</ce:surname><ce:given-name>S.C.</ce:given-name></sb:author><sb:author><ce:surname>Farrant</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>Bryant</ce:surname><ce:given-name>A.</ce:given-name></sb:author><sb:author><ce:surname>Edwards</ce:surname><ce:given-name>A.J.</ce:given-name></sb:author><sb:author><ce:surname>Burman</ce:surname><ce:given-name>S.</ce:given-name></sb:author><sb:author><ce:surname>Lever</ce:surname><ce:given-name>A.</ce:given-name></sb:author><sb:author><ce:surname>Clarke</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>Webster</ce:surname><ce:given-name>A.D.B.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Non-adherent, low-density cells from human peripheral blood contain dendritic cells and monocytes, both with veiled morphology</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Immunology</sb:maintitle></sb:title><sb:volume-nr>57</sb:volume-nr></sb:series><sb:date>1986</sb:date></sb:issue><sb:pages><sb:first-page>595</sb:first-page><sb:last-page>603</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib16"><ce:label>Kurane, et al, 1986</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Kurane</ce:surname><ce:given-name>I.</ce:given-name></sb:author><sb:author><ce:surname>Meager</ce:surname><ce:given-name>A.</ce:given-name></sb:author><sb:author><ce:surname>Ennis</ce:surname><ce:given-name>F.A.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Induction of interferon alpha and gamma from human lymphocytes by Dengue virus-infected cells</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. gen. Virol.</sb:maintitle></sb:title><sb:volume-nr>67</sb:volume-nr></sb:series><sb:date>1986</sb:date></sb:issue><sb:pages><sb:first-page>1653</sb:first-page><sb:last-page>1661</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib17"><ce:label>L'Haridon, et al, 1991</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>L'Haridon</ce:surname><ce:given-name>R.</ce:given-name></sb:author><sb:author><ce:surname>Bourget</ce:surname><ce:given-name>P.</ce:given-name></sb:author><sb:author><ce:surname>Lefèvre</ce:surname><ce:given-name>F.</ce:given-name></sb:author><sb:author><ce:surname>La Bonnardière</ce:surname><ce:given-name>C.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Production of a hybridoma library to recombinant porcine alpha I interferon: a very sensitive assay (ISBBA) allows the detection of a large number of clones</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Hybridoma</sb:maintitle></sb:title><sb:volume-nr>10</sb:volume-nr></sb:series><sb:date>1991</sb:date></sb:issue><sb:pages><sb:first-page>35</sb:first-page><sb:last-page>47</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib18"><ce:label>La Bonnardière and Laude, 1981</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>La Bonnardière</ce:surname><ce:given-name>C.</ce:given-name></sb:author><sb:author><ce:surname>Laude</ce:surname><ce:given-name>H.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>High interferon titer in newborn pig intestine during experimentally induced viral enteritis</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Infect. Immun.</sb:maintitle></sb:title><sb:volume-nr>32</sb:volume-nr></sb:series><sb:date>1981</sb:date></sb:issue><sb:pages><sb:first-page>28</sb:first-page><sb:last-page>31</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib19"><ce:label>Laude, et al, 1992</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Laude</ce:surname><ce:given-name>H.</ce:given-name></sb:author><sb:author><ce:surname>Gelfi</ce:surname><ce:given-name>J.</ce:given-name></sb:author><sb:author><ce:surname>Lavenant</ce:surname><ce:given-name>L.</ce:given-name></sb:author><sb:author><ce:surname>Charley</ce:surname><ce:given-name>B.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Single amino acid changes in the viral glycoprotein M affect induction of alpha interferon by coronavirus TGEV</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Virol.</sb:maintitle></sb:title><sb:volume-nr>66</sb:volume-nr></sb:series><sb:date>1992</sb:date></sb:issue><sb:pages><sb:first-page>743</sb:first-page><sb:last-page>749</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib20"><ce:label>Lebon, 1985</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Lebon</ce:surname><ce:given-name>P.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Inhibition of Herpes Simplex virus type 1-induced interferon synthesis by monoclonal antibodies against viral glycoprotein D and by lysosomotropic drugs</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. gen. Virol.</sb:maintitle></sb:title><sb:volume-nr>66</sb:volume-nr></sb:series><sb:date>1985</sb:date></sb:issue><sb:pages><sb:first-page>2781</sb:first-page><sb:last-page>2786</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib21"><ce:label>Lebon, et al, 1982</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Lebon</ce:surname><ce:given-name>P.</ce:given-name></sb:author><sb:author><ce:surname>Commoy-Chevalier</ce:surname><ce:given-name>M.J.</ce:given-name></sb:author><sb:author><ce:surname>Robert-Galliot</ce:surname><ce:given-name>B.</ce:given-name></sb:author><sb:author><ce:surname>Chany</ce:surname><ce:given-name>C.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Different mechanisms for α and β interferon induction</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Virology</sb:maintitle></sb:title><sb:volume-nr>119</sb:volume-nr></sb:series><sb:date>1982</sb:date></sb:issue><sb:pages><sb:first-page>504</sb:first-page><sb:last-page>507</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib22"><ce:label>Lefèvre, et al, 1990</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Lefèvre</ce:surname><ce:given-name>F.</ce:given-name></sb:author><sb:author><ce:surname>L'Haridon</ce:surname><ce:given-name>R.</ce:given-name></sb:author><sb:author><ce:surname>Borras-Cuesta</ce:surname><ce:given-name>F.</ce:given-name></sb:author><sb:author><ce:surname>La Bonnardière</ce:surname><ce:given-name>C.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Production, purification and biological properties of an <ce:italic>Escherichia coli</ce:italic>-derived recombinant porcine alpha interferon</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. gen. Virol.</sb:maintitle></sb:title><sb:volume-nr>71</sb:volume-nr></sb:series><sb:date>1990</sb:date></sb:issue><sb:pages><sb:first-page>1057</sb:first-page><sb:last-page>1063</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib23"><ce:label>Lunney and Pescovitz, 1987</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Lunney</ce:surname><ce:given-name>J.K.</ce:given-name></sb:author><sb:author><ce:surname>Pescovitz</ce:surname><ce:given-name>M.D.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Phenotypic and functional characterization of pig lymphocyte populations</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Vet. Immunol. Immunopathol.</sb:maintitle></sb:title><sb:volume-nr>17</sb:volume-nr></sb:series><sb:date>1987</sb:date></sb:issue><sb:pages><sb:first-page>135</sb:first-page><sb:last-page>144</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib24"><ce:label>Sandberg, et al, 1989</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Sandberg</ce:surname><ce:given-name>K.</ce:given-name></sb:author><sb:author><ce:surname>Gobl</ce:surname><ce:given-name>A.E.</ce:given-name></sb:author><sb:author><ce:surname>Funa</ce:surname><ce:given-name>K.</ce:given-name></sb:author><sb:author><ce:surname>Alm</ce:surname><ce:given-name>G.V</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Characterization of the blood mononuclear leucocytes producting alpha interferon after stimulation with herpes simplex virus <ce:italic>in vitro</ce:italic>, by means of combined immunohistochemical staining and in situ RNA-RNA hybridization</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>Scand. J. Immunol.</sb:maintitle></sb:title><sb:volume-nr>29</sb:volume-nr></sb:series><sb:date>1989</sb:date></sb:issue><sb:pages><sb:first-page>651</sb:first-page><sb:last-page>658</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib25"><ce:label>Sandberg, et al, 1990</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Sandberg</ce:surname><ce:given-name>K.</ce:given-name></sb:author><sb:author><ce:surname>Matsson</ce:surname><ce:given-name>P.</ce:given-name></sb:author><sb:author><ce:surname>Alm</ce:surname><ce:given-name>G.V.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>A distinct population of non-phagocytic and low level CD4<ce:sup loc="post">+</ce:sup> null lymphocytes produce IFN-α after stimulation by herpes simplex virus-infected cells</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Immunol.</sb:maintitle></sb:title><sb:volume-nr>145</sb:volume-nr></sb:series><sb:date>1990</sb:date></sb:issue><sb:pages><sb:first-page>1015</sb:first-page><sb:last-page>1020</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference><ce:bib-reference id="bib26"><ce:label>Stewart, et al, 1971</ce:label><sb:reference><sb:contribution langtype="en"><sb:authors><sb:author><ce:surname>Stewart</ce:surname><ce:given-name>W.E.</ce:given-name></sb:author><sb:author><ce:surname>Gosser</ce:surname><ce:given-name>L.B.</ce:given-name></sb:author><sb:author><ce:surname>Lockhart</ce:surname><ce:given-name>R.Z.</ce:given-name></sb:author></sb:authors><sb:title><sb:maintitle>Priming: a non-antiviral function of interferon</sb:maintitle></sb:title></sb:contribution><sb:host><sb:issue><sb:series><sb:title><sb:maintitle>J. Virol.</sb:maintitle></sb:title><sb:volume-nr>7</sb:volume-nr></sb:series><sb:date>1971</sb:date></sb:issue><sb:pages><sb:first-page>792</sb:first-page><sb:last-page>801</sb:last-page></sb:pages></sb:host></sb:reference></ce:bib-reference></ce:bibliography-sec></ce:bibliography></tail></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Enrichment of coronavirus-induced interferon-producing blood leukocytes increases the interferon yield per cell: A study with pig leukocytes</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Enrichment of coronavirus-induced interferon-producing blood leukocytes increases the interferon yield per cell: A study with pig leukocytes</title></titleInfo><name type="personal"><namePart type="given">W.</namePart><namePart type="family">Nowacki</namePart><affiliation>Laboratoire de Virologie et Immunologie moléculaires, INRA, 78350 Jouy-en-Josas (France)</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">B.</namePart><namePart type="family">Charley</namePart><affiliation>Laboratoire de Virologie et Immunologie moléculaires, INRA, 78350 Jouy-en-Josas (France)</affiliation><description>Corresponding author.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued><copyrightDate encoding="w3cdtf">1993</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Summary: Porcine peripheral blood mononuclear cells, which secrete IFNα in response to a coronavirus, transmissible gastroenteritis virus, were detected by a filter immunoplaque assay (ELISPOT). IFNα-producing cells (IPC), which are present at a low frequency in the blood, could be enriched up to 100-fold by sequential depletion of plastic-adherent cells and cell fractionation on metrizamide density gradients. IPC were present in the non-adherent low-density cell subpopulation. Cell selection experiments using antibody (Ab)-coated immunomagnetic beads revealed that porcine IPC could be positively selected by anti-CD4 or -SLA-class-II Ab, but not by anti-CD2 or -CD8 Ab. The estimated IFN yield per IPC was found to increase when IPC were assayed at higher concentrations. These data suggest that IPC represent a unique and distinct cell population in the blood, which could secrete higher amounts of IFN following its accumulation at a site of viral infection.</abstract><abstract lang="fr">fr: L'enrichissement en leucocytes sanguins producteurs d'interféron après induction par un coronavirus accroît la quantité d'interféron produite par celluleLes cellules mononucléées du sang périphérique du porc qui sécrètent l'IFNα après induction par le coronavirus de la gastroentérite transmissible, sont détectables par une technique immunoenzymatique sur filtre (ELISPOT). Ces cellules sont très peu fréquentes dans le sang mais ont pu être enrichies par déplétion des cellules adhérentes au plastique suivie d'une séparation cellulaire sur gradient de métrizamide. Les cellules sécrétrices d'IFNα ont été enrichies dans la fraction de faible densité des cellules non adhérentes. Des expériences de sélection des cellules à l'aide de billes magnétiques recouvertes d'anticorps ont montré que ces cellules étaient positivement sélectionnées par des anticorps anti-CD4 ou anti-SLA-classe-II mais non par des anticorps anti-CD2 ou -CD8. La production estimée d'IFN par cellule sécrétrice a augmenté dans les diverses situations où ces cellules se trouvaient être plus concentrées. Ces résultats suggèrent que les cellules sécrétrices d'IFNα représentent une sous-population cellulaire particulière des cellules sanguines, dont la capacité à produire l'IFN pourrait être accrue du fait de leur accumulation aux sites d'infection virale.</abstract><note type="content">Section title: Original article</note><subject lang="en"><topic>Coronavirus</topic><topic>Leukocyte</topic><topic>Interferon alpha</topic><topic>Transmissible gastroenteritis virus</topic><topic>IPC</topic><topic>ELISPOT</topic><topic>Immunobeads</topic><topic>PBMC</topic><topic>mAb</topic></subject><subject lang="fr"><genre>Motsclés</genre><topic>Leucocyte</topic><topic>Coronavirus</topic><topic>Interféron alpha</topic><topic>Virus de la gastroentérite transmissible</topic><topic>Cellules productrices d'interféron</topic><topic>ELISPOT</topic><topic>Billes immunomagnétiques</topic><topic>PBMC</topic><topic>Anticorps monoclonaux</topic></subject><relatedItem type="host"><titleInfo><title>Research in Immunology</title></titleInfo><titleInfo type="abbreviated"><title>X83</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1993</dateIssued></originInfo><identifier type="ISSN">0923-2494</identifier><identifier type="PII">S0923-2494(00)X0032-6</identifier><part><date>1993</date><detail type="volume"><number>144</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>87</start><end>167</end></extent><extent unit="pages"><start>111</start><end>120</end></extent></part></relatedItem><identifier type="istex">DF6FABE0FB3C0EC34AF0AD7BDD3C1ECE62B5AB06</identifier><identifier type="ark">ark:/67375/6H6-5V4VS29N-K</identifier><identifier type="DOI">10.1016/0923-2494(93)80066-8</identifier><identifier type="PII">0923-2494(93)80066-8</identifier><identifier type="ArticleID">93800668</identifier><accessCondition type="use and reproduction" contentType="copyright">©1993 Institut Pasteur/Elsevier Paris</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Institut Pasteur/Elsevier Paris, ©1993</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-5V4VS29N-K/record.json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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