Neurovirulence of six different murine coronavirus JHMV variants for rats
Identifieur interne : 000099 ( Istex/Corpus ); précédent : 000098; suivant : 000100Neurovirulence of six different murine coronavirus JHMV variants for rats
Auteurs : Matsubara Yutaka ; Watanabe Rihito ; Taguchi FumihiroSource :
- Virus Research [ 0168-1702 ] ; 1991.
English descriptors
- Teeft :
- Cnsv, Coronavirus, Demyelinating, Demyelinating disease, Demyelinating encephalomyelitis, Determinant, Encephalomyelitis, Fleming, Glial, Glial cells, Infectious virus, Inoculated, Intracerebrally, Intracerebrally inoculated, Jhmv, Jhmv infection, Jhmv variants, Large mrna3, Lesion, Lewis rats, Massa, Meulen, Monoclonal, Monoclonal antibodies, Morbidity, Murine, Murine coronavirus, Nagashima, Neuron, Neurovirulence, Plaque, Previous studies, Rat, Rats inoculated, Sorensen, Spinal, Spinal cord, Stohlman, Taguchi, Variant, Variant viruses, Various titers, Viral, Viral antigen, Viral antigens, Virology, Virus infection, Wege.
Abstract
Abstract: Six variant viruses of the JHMV strain of murine coronavirus with large (cl-2, CNSV, DL and DS) or small (sp-4 and JHM-X) S proteins were compared in terms of their relative neurovirulence in weanling Lewis rats. Inoculation of various doses of the variants revealed that the cl-2 and CNSV were highly virulent and DL and DS were low-virulent, while sp-4 and JHM-X were avirulent. Pathological examination of rats infected with variants cl-2, DL and sp-4 showed that the cl-2 and DL induced severe and mild acute encephalomyelitis, respectively, while no lesions were observed in the central nervous system of rats infected with sp-4. Virus growth and distribution of antigen in rat brains correlated strongly with neurovirulence. These results suggest that S protein plays a role in neurovirulence in rats. In addition, these variant viruses were shown to be useful tools for further analysis of JHMV neurovirulence in animals as well as in cultured cells.
Url:
DOI: 10.1016/0168-1702(91)90060-9
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ISTEX:69BA27A0D9342A8425702E9CB923C2312A7ED9D3Le document en format XML
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Inoculation of various doses of the variants revealed that the cl-2 and CNSV were highly virulent and DL and DS were low-virulent, while sp-4 and JHM-X were avirulent. Pathological examination of rats infected with variants cl-2, DL and sp-4 showed that the cl-2 and DL induced severe and mild acute encephalomyelitis, respectively, while no lesions were observed in the central nervous system of rats infected with sp-4. Virus growth and distribution of antigen in rat brains correlated strongly with neurovirulence. These results suggest that S protein plays a role in neurovirulence in rats. In addition, these variant viruses were shown to be useful tools for further analysis of JHMV neurovirulence in animals as well as in cultured cells.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>jhmv</json:string><json:string>inoculated</json:string><json:string>neurovirulence</json:string><json:string>viral</json:string><json:string>taguchi</json:string><json:string>wege</json:string><json:string>coronavirus</json:string><json:string>demyelinating</json:string><json:string>cnsv</json:string><json:string>meulen</json:string><json:string>murine</json:string><json:string>jhmv variants</json:string><json:string>glial</json:string><json:string>rats inoculated</json:string><json:string>sorensen</json:string><json:string>encephalomyelitis</json:string><json:string>spinal cord</json:string><json:string>demyelinating disease</json:string><json:string>viral antigen</json:string><json:string>massa</json:string><json:string>spinal</json:string><json:string>morbidity</json:string><json:string>monoclonal</json:string><json:string>lewis rats</json:string><json:string>stohlman</json:string><json:string>intracerebrally</json:string><json:string>virology</json:string><json:string>monoclonal antibodies</json:string><json:string>nagashima</json:string><json:string>variant</json:string><json:string>neuron</json:string><json:string>jhmv infection</json:string><json:string>intracerebrally inoculated</json:string><json:string>glial cells</json:string><json:string>rat</json:string><json:string>virus infection</json:string><json:string>viral antigens</json:string><json:string>murine coronavirus</json:string><json:string>lesion</json:string><json:string>determinant</json:string><json:string>infectious virus</json:string><json:string>large mrna3</json:string><json:string>previous studies</json:string><json:string>various titers</json:string><json:string>variant viruses</json:string><json:string>demyelinating encephalomyelitis</json:string><json:string>fleming</json:string><json:string>plaque</json:string></teeft></keywords><author><json:item><name>Matsubara Yutaka</name><affiliations><json:string>National Institute of Neuroscience, NCNP, Tokyo, Japan</json:string></affiliations></json:item><json:item><name>Watanabe Rihito</name><affiliations><json:string>National Institute of Neuroscience, NCNP, Tokyo, Japan</json:string></affiliations></json:item><json:item><name>Taguchi Fumihiro</name><affiliations><json:string>National Institute of Neuroscience, NCNP, Tokyo, Japan</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Murine coronavirus JHM</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>Neurovirulence</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>S protein</value></json:item></subject><arkIstex>ark:/67375/6H6-BP4MTCJ8-B</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Six variant viruses of the JHMV strain of murine coronavirus with large (cl-2, CNSV, DL and DS) or small (sp-4 and JHM-X) S proteins were compared in terms of their relative neurovirulence in weanling Lewis rats. Inoculation of various doses of the variants revealed that the cl-2 and CNSV were highly virulent and DL and DS were low-virulent, while sp-4 and JHM-X were avirulent. Pathological examination of rats infected with variants cl-2, DL and sp-4 showed that the cl-2 and DL induced severe and mild acute encephalomyelitis, respectively, while no lesions were observed in the central nervous system of rats infected with sp-4. Virus growth and distribution of antigen in rat brains correlated strongly with neurovirulence. These results suggest that S protein plays a role in neurovirulence in rats. In addition, these variant viruses were shown to be useful tools for further analysis of JHMV neurovirulence in animals as well as in cultured cells.</abstract><qualityIndicators><refBibsNative>true</refBibsNative><abstractWordCount>155</abstractWordCount><abstractCharCount>967</abstractCharCount><keywordCount>3</keywordCount><score>7.721</score><pdfWordCount>3861</pdfWordCount><pdfCharCount>26440</pdfCharCount><pdfVersion>1.4</pdfVersion><pdfPageCount>14</pdfPageCount><pdfPageSize>432 x 648 pts</pdfPageSize></qualityIndicators><title>Neurovirulence of six different murine coronavirus JHMV variants for rats</title><pmid><json:string>1656623</json:string></pmid><pii><json:string>0168-1702(91)90060-9</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Virus Research</title><language><json:string>unknown</json:string></language><publicationDate>1991</publicationDate><issn><json:string>0168-1702</json:string></issn><pii><json:string>S0168-1702(00)X0170-0</json:string></pii><volume>20</volume><issue>1</issue><pages><first>45</first><last>58</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>10s</json:string><json:string>2/5/5</json:string><json:string>3/4/5</json:string><json:string>2/2/5</json:string><json:string>3/5/5</json:string><json:string>4/4/5</json:string><json:string>70s</json:string><json:string>1991</json:string></date><geogName></geogName><orgName><json:string>Ministry of Health and Welfare of Japan</json:string><json:string>National Institute of Animal Health, Ministry of Agriculture, Forestry and Fisheries</json:string><json:string>University of Southern California</json:string><json:string>National Institute of Neuroscience, NCNP</json:string><json:string>Grants-in-Aid</json:string></orgName><orgName_funder><json:string>Grants-in-Aid</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>H. Hirano</json:string><json:string>K. Yamaguchi</json:string><json:string>Jayne K. Makino</json:string><json:string>Jennifer A. Fosmire</json:string><json:string>J.O. Fleming</json:string><json:string>Shinji</json:string><json:string>Evidence</json:string><json:string>Y. Kumagai</json:string><json:string>S. Makino</json:string><json:string>S.A. Stohlman</json:string></persName><placeName><json:string>Mannheim</json:string><json:string>Japan</json:string><json:string>Dublin</json:string><json:string>CA</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Fleming et al., 1986, 1987</json:string><json:string>Massa et al., 1986b</json:string><json:string>Beushausen et al., 1987</json:string><json:string>Knobler et al., 1981</json:string><json:string>Beushausen and Dales, 1985</json:string><json:string>Morris et al. (1989)</json:string><json:string>Taguchi et al., 1980</json:string><json:string>Wege et al., 1981</json:string><json:string>Stohlman et al., 1982</json:string><json:string>Watanabe et al., 1987</json:string><json:string>Taguchi et al., 1985</json:string><json:string>Watanabe et al., 1983, 1987</json:string><json:string>Van Noorden and Polak, 1983</json:string><json:string>Massa et al., 1986a</json:string><json:string>Dalziel et al., 1986</json:string><json:string>Sorensen et al., 1980</json:string><json:string>Makino et al., 1984</json:string><json:string>Hondo et al., 1982</json:string><json:string>Sorensen et al., 1982, 1987</json:string><json:string>Stohlman and Weiner, 1981</json:string><json:string>Wege et al., 1984b</json:string><json:string>Sorensen et al., 1984</json:string><json:string>Nagashima et al., 1978b</json:string><json:string>Sorensen and Dales, 1985</json:string><json:string>Holmes et al., 1981</json:string><json:string>Taguchi and Fleming, 1989</json:string><json:string>Holland et al., 1982</json:string><json:string>Taguchi et al., 1985, 1986</json:string><json:string>Wege et al., 1984a</json:string><json:string>Schmidt et al., 1987</json:string><json:string>Fleming et al., 1987</json:string><json:string>Nagashima et al., 1978a</json:string><json:string>Wege et al., 1988</json:string><json:string>Collins et al., 1982</json:string><json:string>Fleming et al., 1986</json:string><json:string>Massa et al., 1988</json:string><json:string>Koga et al., 1984</json:string><json:string>Taguchi et al., 1986</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-BP4MTCJ8-B</json:string></ark><categories><wos><json:string>1 - 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Taguchi, National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi-machi, Kodaira, Tokyo 187, Japan.</p></note><affiliation>National Institute of Neuroscience, NCNP, Tokyo, Japan</affiliation></author><idno type="istex">69BA27A0D9342A8425702E9CB923C2312A7ED9D3</idno><idno type="ark">ark:/67375/6H6-BP4MTCJ8-B</idno><idno type="DOI">10.1016/0168-1702(91)90060-9</idno><idno type="PII">0168-1702(91)90060-9</idno></analytic><monogr><title level="j">Virus Research</title><title level="j" type="abbrev">VIRUS</title><idno type="pISSN">0168-1702</idno><idno type="PII">S0168-1702(00)X0170-0</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1991"></date><biblScope unit="volume">20</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="45">45</biblScope><biblScope unit="page" to="58">58</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1991</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Six variant viruses of the JHMV strain of murine coronavirus with large (cl-2, CNSV, DL and DS) or small (sp-4 and JHM-X) S proteins were compared in terms of their relative neurovirulence in weanling Lewis rats. Inoculation of various doses of the variants revealed that the cl-2 and CNSV were highly virulent and DL and DS were low-virulent, while sp-4 and JHM-X were avirulent. Pathological examination of rats infected with variants cl-2, DL and sp-4 showed that the cl-2 and DL induced severe and mild acute encephalomyelitis, respectively, while no lesions were observed in the central nervous system of rats infected with sp-4. Virus growth and distribution of antigen in rat brains correlated strongly with neurovirulence. These results suggest that S protein plays a role in neurovirulence in rats. In addition, these variant viruses were shown to be useful tools for further analysis of JHMV neurovirulence in animals as well as in cultured cells.</p></abstract><textClass><keywords scheme="keyword"><list><head>Keywords</head><item><term>Murine coronavirus JHM</term></item><item><term>Neurovirulence</term></item><item><term>S protein</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1991-02-21">Modified</change><change when="1991">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-BP4MTCJ8-B/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>VIRUS</jid><aid>91900609</aid><ce:pii>0168-1702(91)90060-9</ce:pii><ce:doi>10.1016/0168-1702(91)90060-9</ce:doi><ce:copyright type="unknown" year="1991"></ce:copyright></item-info><head><ce:title>Neurovirulence of six different murine coronavirus JHMV variants for rats</ce:title><ce:author-group><ce:author><ce:surname>Yutaka</ce:surname><ce:given-name>Matsubara</ce:given-name><ce:cross-ref refid="FN1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:author><ce:surname>Rihito</ce:surname><ce:given-name>Watanabe</ce:given-name></ce:author><ce:author><ce:surname>Fumihiro</ce:surname><ce:given-name>Taguchi</ce:given-name><ce:cross-ref refid="COR1"><ce:sup>∗</ce:sup></ce:cross-ref></ce:author><ce:affiliation><ce:textfn>National Institute of Neuroscience, NCNP, Tokyo, Japan</ce:textfn></ce:affiliation><ce:correspondence id="COR1"><ce:label>∗</ce:label><ce:text>Correspondence to: F. Taguchi, National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi-machi, Kodaira, Tokyo 187, Japan.</ce:text></ce:correspondence><ce:footnote id="FN1"><ce:label>∗</ce:label><ce:note-para>Present address: National Institute of Animal Health, Ministry of Agriculture, Forestry and Fisheries, 3-1-1 Kannondai, Tsukuba, Ibaragi 305.</ce:note-para></ce:footnote></ce:author-group><ce:date-received day="18" month="12" year="1990"></ce:date-received><ce:date-revised day="21" month="2" year="1991"></ce:date-revised><ce:date-accepted day="26" month="2" year="1991"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Six variant viruses of the JHMV strain of murine coronavirus with large (cl-2, CNSV, DL and DS) or small (sp-4 and JHM-X) S proteins were compared in terms of their relative neurovirulence in weanling Lewis rats. Inoculation of various doses of the variants revealed that the cl-2 and CNSV were highly virulent and DL and DS were low-virulent, while sp-4 and JHM-X were avirulent. Pathological examination of rats infected with variants cl-2, DL and sp-4 showed that the cl-2 and DL induced severe and mild acute encephalomyelitis, respectively, while no lesions were observed in the central nervous system of rats infected with sp-4. Virus growth and distribution of antigen in rat brains correlated strongly with neurovirulence. These results suggest that S protein plays a role in neurovirulence in rats. In addition, these variant viruses were shown to be useful tools for further analysis of JHMV neurovirulence in animals as well as in cultured cells.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords><ce:section-title>Keywords</ce:section-title><ce:keyword><ce:text>Murine coronavirus JHM</ce:text></ce:keyword><ce:keyword><ce:text>Neurovirulence</ce:text></ce:keyword><ce:keyword><ce:text>S protein</ce:text></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Neurovirulence of six different murine coronavirus JHMV variants for rats</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Neurovirulence of six different murine coronavirus JHMV variants for rats</title></titleInfo><name type="personal"><namePart type="given">Matsubara</namePart><namePart type="family">Yutaka</namePart><affiliation>National Institute of Neuroscience, NCNP, Tokyo, Japan</affiliation><description>Present address: National Institute of Animal Health, Ministry of Agriculture, Forestry and Fisheries, 3-1-1 Kannondai, Tsukuba, Ibaragi 305.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Watanabe</namePart><namePart type="family">Rihito</namePart><affiliation>National Institute of Neuroscience, NCNP, Tokyo, Japan</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Taguchi</namePart><namePart type="family">Fumihiro</namePart><affiliation>National Institute of Neuroscience, NCNP, Tokyo, Japan</affiliation><description>Correspondence to: F. Taguchi, National Institute of Neuroscience, NCNP, 4-1-1 Ogawahigashi-machi, Kodaira, Tokyo 187, Japan.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1991</dateIssued><dateModified encoding="w3cdtf">1991-02-21</dateModified><copyrightDate encoding="w3cdtf">1991</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Six variant viruses of the JHMV strain of murine coronavirus with large (cl-2, CNSV, DL and DS) or small (sp-4 and JHM-X) S proteins were compared in terms of their relative neurovirulence in weanling Lewis rats. Inoculation of various doses of the variants revealed that the cl-2 and CNSV were highly virulent and DL and DS were low-virulent, while sp-4 and JHM-X were avirulent. Pathological examination of rats infected with variants cl-2, DL and sp-4 showed that the cl-2 and DL induced severe and mild acute encephalomyelitis, respectively, while no lesions were observed in the central nervous system of rats infected with sp-4. Virus growth and distribution of antigen in rat brains correlated strongly with neurovirulence. These results suggest that S protein plays a role in neurovirulence in rats. In addition, these variant viruses were shown to be useful tools for further analysis of JHMV neurovirulence in animals as well as in cultured cells.</abstract><subject><genre>Keywords</genre><topic>Murine coronavirus JHM</topic><topic>Neurovirulence</topic><topic>S protein</topic></subject><relatedItem type="host"><titleInfo><title>Virus Research</title></titleInfo><titleInfo type="abbreviated"><title>VIRUS</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1991</dateIssued></originInfo><identifier type="ISSN">0168-1702</identifier><identifier type="PII">S0168-1702(00)X0170-0</identifier><part><date>1991</date><detail type="volume"><number>20</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>106</end></extent><extent unit="pages"><start>45</start><end>58</end></extent></part></relatedItem><identifier type="istex">69BA27A0D9342A8425702E9CB923C2312A7ED9D3</identifier><identifier type="ark">ark:/67375/6H6-BP4MTCJ8-B</identifier><identifier type="DOI">10.1016/0168-1702(91)90060-9</identifier><identifier type="PII">0168-1702(91)90060-9</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-BP4MTCJ8-B/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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