Molecular Screening by Polymerase Chain Reaction Detects Panleukopenia Virus DNA in Formalin-Fixed Hearts from Cats with Idiopathic Cardiomyopathy and Myocarditis
Identifieur interne : 000055 ( Istex/Corpus ); précédent : 000054; suivant : 000056Molecular Screening by Polymerase Chain Reaction Detects Panleukopenia Virus DNA in Formalin-Fixed Hearts from Cats with Idiopathic Cardiomyopathy and Myocarditis
Auteurs : Kathryn M. Meurs ; Philip R. Fox ; Alexander L. Magnon ; Si-Kwang Liu ; Jeffrey A. TowbinSource :
- Cardiovascular Pathology [ 1054-8807 ] ; 2000.
English descriptors
- Teeft :
- Animal model, Arrhythmogenic right, Calici, Calici virus, Canine, Canine parvovirus, Cardiac, Cardiomyopathy, Cardiovasc, Cardiovasc pathol, Cell cultures, Coll cardiol, Control cats, Corona, Corona virus, Endomyocardial biopsies, Etiology, Feline, Feline cardiomyopathy, Feline panleukopenia, Feline panleukopenia virus, Genome, Heart failure, Herpes, Herpes virus, Histopathologic, Histopathologic evidence, Human disease, Hypertrophic, Hypertrophic cardiomyopathy, Idiopathic, Idiopathic cardiomyopathy, Inflammation, Light microscopy, Meurs, Molecular detection, Myocardial, Myocardial tissue, Myocarditis, Myocarditis years, Myocardium, Nonstructural protein, Panleukopenia, Panleukopenia virus, Panleukopenia virus genome, Parvovirus, Pathogenesis, Pathol, Polymerase, Polymerase chain reaction, Positive control, Primer, Restrictive cardiomyopathy, Sudden death, Towbin, Ventricular, Viral, Viral genome, Viral infection, Viral myocarditis.
Abstract
Abstract: Viral myocarditis has been suggested as an etiology for cardiomyopathy in several mammalian species. Myocarditis and idiopathic cardiomyopathy have been reported in the domestic cat, although a viral etiology has not been demonstrated. Because of the continuing interest in the potential relationship between viral myocarditis and cardiomyopathy, we evaluated hearts from cats with spontaneous, idiopathic cardiomyopathy for viral genomic material within myocytes by polymerase chain reaction, and for the presence of myocarditis by light microscopy. Thirty-one (31) formalin-fixed hearts from domestic cats who died of idiopathic cardiomyopathy were randomly selected from pathology archives. Seventeen (17) formalin-fixed hearts from healthy cats were similarly selected as normal controls. The polymerase chain reaction (PCR) was used to evaluate myocardial tissue for the presence of viral genome from feline panleukopenia virus, herpes virus, calici virus, and corona virus. Hearts were examined using light microscopy for histologic evidence of myocarditis according to the Dallas criteria. Panleukopenia virus was identified by PCR in 10 of 31 cats with cardiomyopathy but in none of the controls. Neither cardiomyopathic or control cats tested positive by PCR for herpes virus, calici virus, and corona virus. Myocarditis was detected by histologic examination in 18 of 31 cardiomyopathic cats and in none of 17 control cats. Myocarditis and or feline panleukopenia virus genome was detected in felines with idiopathic hypertrophic, dilated, and restrictive cardiomyopathy, suggesting a possible role of viral infection and inflammation in the pathogenesis of cardiomyopathy in this species.
Url:
DOI: 10.1016/S1054-8807(00)00031-4
Links to Exploration step
ISTEX:69891B542A0AF2EC24913E6EECB25679F37BBAD2Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Molecular Screening by Polymerase Chain Reaction Detects Panleukopenia Virus DNA in Formalin-Fixed Hearts from Cats with Idiopathic Cardiomyopathy and Myocarditis</title><author><name sortKey="Meurs, Kathryn M" sort="Meurs, Kathryn M" uniqKey="Meurs K" first="Kathryn M" last="Meurs">Kathryn M. Meurs</name><affiliation><mods:affiliation>Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Address for correspondence: Dr. Kathryn M. Meurs, Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, 610 Vernon L. Tharp Street, Columbus, Ohio 43210, USA. Tel: 614-292-3551; Fax: 614-292-0895</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: meurs.1@osu.edu</mods:affiliation></affiliation></author><author><name sortKey="Fox, Philip R" sort="Fox, Philip R" uniqKey="Fox P" first="Philip R" last="Fox">Philip R. Fox</name><affiliation><mods:affiliation>Caspary Research Institute of The Animal Medical Center, New York, NY, USA</mods:affiliation></affiliation></author><author><name sortKey="Magnon, Alexander L" sort="Magnon, Alexander L" uniqKey="Magnon A" first="Alexander L" last="Magnon">Alexander L. Magnon</name><affiliation><mods:affiliation>Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA</mods:affiliation></affiliation></author><author><name sortKey="Liu, Si Kwang" sort="Liu, Si Kwang" uniqKey="Liu S" first="Si-Kwang" last="Liu">Si-Kwang Liu</name><affiliation><mods:affiliation>Caspary Research Institute of The Animal Medical Center, New York, NY, USA</mods:affiliation></affiliation></author><author><name sortKey="Towbin, Jeffrey A" sort="Towbin, Jeffrey A" uniqKey="Towbin J" first="Jeffrey A" last="Towbin">Jeffrey A. Towbin</name><affiliation><mods:affiliation>Baylor College of Medicine, Houston, TX, USA</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:69891B542A0AF2EC24913E6EECB25679F37BBAD2</idno><date when="2000" year="2000">2000</date><idno type="doi">10.1016/S1054-8807(00)00031-4</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-Q76BDC1K-2/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000055</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000055</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Molecular Screening by Polymerase Chain Reaction Detects Panleukopenia Virus DNA in Formalin-Fixed Hearts from Cats with Idiopathic Cardiomyopathy and Myocarditis</title><author><name sortKey="Meurs, Kathryn M" sort="Meurs, Kathryn M" uniqKey="Meurs K" first="Kathryn M" last="Meurs">Kathryn M. Meurs</name><affiliation><mods:affiliation>Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA</mods:affiliation></affiliation><affiliation><mods:affiliation>Address for correspondence: Dr. Kathryn M. Meurs, Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, 610 Vernon L. Tharp Street, Columbus, Ohio 43210, USA. Tel: 614-292-3551; Fax: 614-292-0895</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: meurs.1@osu.edu</mods:affiliation></affiliation></author><author><name sortKey="Fox, Philip R" sort="Fox, Philip R" uniqKey="Fox P" first="Philip R" last="Fox">Philip R. Fox</name><affiliation><mods:affiliation>Caspary Research Institute of The Animal Medical Center, New York, NY, USA</mods:affiliation></affiliation></author><author><name sortKey="Magnon, Alexander L" sort="Magnon, Alexander L" uniqKey="Magnon A" first="Alexander L" last="Magnon">Alexander L. Magnon</name><affiliation><mods:affiliation>Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA</mods:affiliation></affiliation></author><author><name sortKey="Liu, Si Kwang" sort="Liu, Si Kwang" uniqKey="Liu S" first="Si-Kwang" last="Liu">Si-Kwang Liu</name><affiliation><mods:affiliation>Caspary Research Institute of The Animal Medical Center, New York, NY, USA</mods:affiliation></affiliation></author><author><name sortKey="Towbin, Jeffrey A" sort="Towbin, Jeffrey A" uniqKey="Towbin J" first="Jeffrey A" last="Towbin">Jeffrey A. Towbin</name><affiliation><mods:affiliation>Baylor College of Medicine, Houston, TX, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Cardiovascular Pathology</title><title level="j" type="abbrev">CVP</title><idno type="ISSN">1054-8807</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000">2000</date><biblScope unit="volume">9</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="119">119</biblScope><biblScope unit="page" to="126">126</biblScope></imprint><idno type="ISSN">1054-8807</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1054-8807</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Animal model</term><term>Arrhythmogenic right</term><term>Calici</term><term>Calici virus</term><term>Canine</term><term>Canine parvovirus</term><term>Cardiac</term><term>Cardiomyopathy</term><term>Cardiovasc</term><term>Cardiovasc pathol</term><term>Cell cultures</term><term>Coll cardiol</term><term>Control cats</term><term>Corona</term><term>Corona virus</term><term>Endomyocardial biopsies</term><term>Etiology</term><term>Feline</term><term>Feline cardiomyopathy</term><term>Feline panleukopenia</term><term>Feline panleukopenia virus</term><term>Genome</term><term>Heart failure</term><term>Herpes</term><term>Herpes virus</term><term>Histopathologic</term><term>Histopathologic evidence</term><term>Human disease</term><term>Hypertrophic</term><term>Hypertrophic cardiomyopathy</term><term>Idiopathic</term><term>Idiopathic cardiomyopathy</term><term>Inflammation</term><term>Light microscopy</term><term>Meurs</term><term>Molecular detection</term><term>Myocardial</term><term>Myocardial tissue</term><term>Myocarditis</term><term>Myocarditis years</term><term>Myocardium</term><term>Nonstructural protein</term><term>Panleukopenia</term><term>Panleukopenia virus</term><term>Panleukopenia virus genome</term><term>Parvovirus</term><term>Pathogenesis</term><term>Pathol</term><term>Polymerase</term><term>Polymerase chain reaction</term><term>Positive control</term><term>Primer</term><term>Restrictive cardiomyopathy</term><term>Sudden death</term><term>Towbin</term><term>Ventricular</term><term>Viral</term><term>Viral genome</term><term>Viral infection</term><term>Viral myocarditis</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: Viral myocarditis has been suggested as an etiology for cardiomyopathy in several mammalian species. Myocarditis and idiopathic cardiomyopathy have been reported in the domestic cat, although a viral etiology has not been demonstrated. Because of the continuing interest in the potential relationship between viral myocarditis and cardiomyopathy, we evaluated hearts from cats with spontaneous, idiopathic cardiomyopathy for viral genomic material within myocytes by polymerase chain reaction, and for the presence of myocarditis by light microscopy. Thirty-one (31) formalin-fixed hearts from domestic cats who died of idiopathic cardiomyopathy were randomly selected from pathology archives. Seventeen (17) formalin-fixed hearts from healthy cats were similarly selected as normal controls. The polymerase chain reaction (PCR) was used to evaluate myocardial tissue for the presence of viral genome from feline panleukopenia virus, herpes virus, calici virus, and corona virus. Hearts were examined using light microscopy for histologic evidence of myocarditis according to the Dallas criteria. Panleukopenia virus was identified by PCR in 10 of 31 cats with cardiomyopathy but in none of the controls. Neither cardiomyopathic or control cats tested positive by PCR for herpes virus, calici virus, and corona virus. Myocarditis was detected by histologic examination in 18 of 31 cardiomyopathic cats and in none of 17 control cats. Myocarditis and or feline panleukopenia virus genome was detected in felines with idiopathic hypertrophic, dilated, and restrictive cardiomyopathy, suggesting a possible role of viral infection and inflammation in the pathogenesis of cardiomyopathy in this species.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>myocarditis</json:string><json:string>cardiomyopathy</json:string><json:string>panleukopenia</json:string><json:string>viral</json:string><json:string>genome</json:string><json:string>pathol</json:string><json:string>polymerase</json:string><json:string>parvovirus</json:string><json:string>hypertrophic</json:string><json:string>herpes</json:string><json:string>viral myocarditis</json:string><json:string>primer</json:string><json:string>idiopathic</json:string><json:string>polymerase chain reaction</json:string><json:string>calici</json:string><json:string>meurs</json:string><json:string>cardiovasc</json:string><json:string>towbin</json:string><json:string>histopathologic</json:string><json:string>feline cardiomyopathy</json:string><json:string>feline</json:string><json:string>corona virus</json:string><json:string>ventricular</json:string><json:string>feline panleukopenia virus</json:string><json:string>panleukopenia virus</json:string><json:string>viral infection</json:string><json:string>viral genome</json:string><json:string>idiopathic cardiomyopathy</json:string><json:string>etiology</json:string><json:string>cardiovasc pathol</json:string><json:string>myocardium</json:string><json:string>corona</json:string><json:string>animal model</json:string><json:string>panleukopenia virus genome</json:string><json:string>canine parvovirus</json:string><json:string>heart failure</json:string><json:string>myocardial tissue</json:string><json:string>hypertrophic cardiomyopathy</json:string><json:string>pathogenesis</json:string><json:string>cardiac</json:string><json:string>herpes virus</json:string><json:string>cell cultures</json:string><json:string>restrictive cardiomyopathy</json:string><json:string>arrhythmogenic right</json:string><json:string>feline panleukopenia</json:string><json:string>control cats</json:string><json:string>myocardial</json:string><json:string>canine</json:string><json:string>inflammation</json:string><json:string>nonstructural protein</json:string><json:string>histopathologic evidence</json:string><json:string>myocarditis years</json:string><json:string>endomyocardial biopsies</json:string><json:string>sudden death</json:string><json:string>positive control</json:string><json:string>calici virus</json:string><json:string>light microscopy</json:string><json:string>molecular detection</json:string><json:string>human disease</json:string><json:string>coll cardiol</json:string></teeft></keywords><author><json:item><name>Kathryn M Meurs DVM, PhD</name><affiliations><json:string>Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA</json:string><json:string>Address for correspondence: Dr. Kathryn M. Meurs, Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, 610 Vernon L. Tharp Street, Columbus, Ohio 43210, USA. Tel: 614-292-3551; Fax: 614-292-0895</json:string><json:string>E-mail: meurs.1@osu.edu</json:string></affiliations></json:item><json:item><name>Philip R Fox DVM</name><affiliations><json:string>Caspary Research Institute of The Animal Medical Center, New York, NY, USA</json:string></affiliations></json:item><json:item><name>Alexander L Magnon BS</name><affiliations><json:string>Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA</json:string></affiliations></json:item><json:item><name>Si-Kwang Liu DVM, PhD</name><affiliations><json:string>Caspary Research Institute of The Animal Medical Center, New York, NY, USA</json:string></affiliations></json:item><json:item><name>Jeffrey A Towbin MD</name><affiliations><json:string>Baylor College of Medicine, Houston, TX, USA</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-Q76BDC1K-2</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: Viral myocarditis has been suggested as an etiology for cardiomyopathy in several mammalian species. Myocarditis and idiopathic cardiomyopathy have been reported in the domestic cat, although a viral etiology has not been demonstrated. Because of the continuing interest in the potential relationship between viral myocarditis and cardiomyopathy, we evaluated hearts from cats with spontaneous, idiopathic cardiomyopathy for viral genomic material within myocytes by polymerase chain reaction, and for the presence of myocarditis by light microscopy. Thirty-one (31) formalin-fixed hearts from domestic cats who died of idiopathic cardiomyopathy were randomly selected from pathology archives. Seventeen (17) formalin-fixed hearts from healthy cats were similarly selected as normal controls. The polymerase chain reaction (PCR) was used to evaluate myocardial tissue for the presence of viral genome from feline panleukopenia virus, herpes virus, calici virus, and corona virus. Hearts were examined using light microscopy for histologic evidence of myocarditis according to the Dallas criteria. Panleukopenia virus was identified by PCR in 10 of 31 cats with cardiomyopathy but in none of the controls. Neither cardiomyopathic or control cats tested positive by PCR for herpes virus, calici virus, and corona virus. Myocarditis was detected by histologic examination in 18 of 31 cardiomyopathic cats and in none of 17 control cats. Myocarditis and or feline panleukopenia virus genome was detected in felines with idiopathic hypertrophic, dilated, and restrictive cardiomyopathy, suggesting a possible role of viral infection and inflammation in the pathogenesis of cardiomyopathy in this species.</abstract><qualityIndicators><refBibsNative>true</refBibsNative><abstractWordCount>241</abstractWordCount><abstractCharCount>1709</abstractCharCount><keywordCount>0</keywordCount><score>9.859</score><pdfWordCount>4967</pdfWordCount><pdfCharCount>31865</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>8</pdfPageCount><pdfPageSize>594 x 792 pts</pdfPageSize></qualityIndicators><title>Molecular Screening by Polymerase Chain Reaction Detects Panleukopenia Virus DNA in Formalin-Fixed Hearts from Cats with Idiopathic Cardiomyopathy and Myocarditis</title><pmid><json:string>10867362</json:string></pmid><pii><json:string>S1054-8807(00)00031-4</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Cardiovascular Pathology</title><language><json:string>unknown</json:string></language><publicationDate>2000</publicationDate><issn><json:string>1054-8807</json:string></issn><pii><json:string>S1054-8807(00)X0030-0</json:string></pii><volume>9</volume><issue>2</issue><pages><first>119</first><last>126</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>2000</json:string></date><geogName></geogName><orgName><json:string>Caspary Research Institute</json:string><json:string>Elsevier Science Inc</json:string><json:string>The Ohio State University</json:string><json:string>Amersham Life Science Inc.</json:string><json:string>Amersham Live Science Inc.</json:string><json:string>Caspary Research Institute of The Animal Medical Center, New York, New York, USA</json:string><json:string>Jandel Corp</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>In</json:string><json:string>Jeffrey A. Towbin</json:string><json:string>Philip R. Fox</json:string><json:string>Alexander L. Magnon</json:string></persName><placeName><json:string>IL</json:string><json:string>Madison</json:string><json:string>USA</json:string><json:string>CA</json:string><json:string>WI</json:string><json:string>Arlington Heights</json:string><json:string>San Rafael</json:string></placeName><ref_url></ref_url><ref_bibl></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-Q76BDC1K-2</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - pathology</json:string><json:string>2 - cardiac & cardiovascular systems</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - clinical medicine</json:string><json:string>3 - cardiovascular system & hematology</json:string></scienceMetrix><scopus><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Cardiology and Cardiovascular Medicine</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - General Medicine</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - Pathology and Forensic Medicine</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string></inist></categories><publicationDate>2000</publicationDate><copyrightDate>2000</copyrightDate><doi><json:string>10.1016/S1054-8807(00)00031-4</json:string></doi><id>69891B542A0AF2EC24913E6EECB25679F37BBAD2</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-Q76BDC1K-2/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-Q76BDC1K-2/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-Q76BDC1K-2/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">Molecular Screening by Polymerase Chain Reaction Detects Panleukopenia Virus DNA in Formalin-Fixed Hearts from Cats with Idiopathic Cardiomyopathy and Myocarditis</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>©2000 Elsevier Science Inc.</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</p></availability><date>2000</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note type="content">Section title: Articles</note><note type="content">Figure 1: Agarose gel electrophoresis of the 397 base pair region of the nonstructural protein 1 of panleukopenia. Lanes 1, 2, 4, 7, 8 contain negative samples from cardiomyopathic cats. Lane 11 is a negative control, lane 12 is a positive control. The initial lane (identified by an asterisk) contains the DNA ladder</note><note type="content">Figure 2: Photomicrographs of representative myocardial sections from cats with cardiomyopathy and myocarditis. (Top) Section from the left ventricular free wall. Extensive mixed inflammatory infiltrate is located predominantly in the subendocardial region consisting of lymphocytes, neutrophils, and plasmacytes. Adjacent myocyte necrosis is severe. Hematoxylin and eosin stain (H&E); magnification ×200. (Bottom) Section from the right auricle. Diffuse neutrophilic infiltrate is associated with extensive myocardial necrosis which is particularly prominent in the subendocardial region. Subendocardial hemorrhage is present. Hematoxylin and eosin stain (H&E); magnification ×100</note><note type="content">Table 1: Polymerase Chain Reaction Conditions for Viral Amplification</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Molecular Screening by Polymerase Chain Reaction Detects Panleukopenia Virus DNA in Formalin-Fixed Hearts from Cats with Idiopathic Cardiomyopathy and Myocarditis</title><author xml:id="author-0000"><persName><forename type="first">Kathryn M</forename><surname>Meurs</surname></persName><roleName type="degree">DVM, PhD</roleName><email>meurs.1@osu.edu</email><affiliation>Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA</affiliation><affiliation>Address for correspondence: Dr. Kathryn M. Meurs, Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, 610 Vernon L. Tharp Street, Columbus, Ohio 43210, USA. Tel: 614-292-3551; Fax: 614-292-0895</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Philip R</forename><surname>Fox</surname></persName><roleName type="degree">DVM</roleName><affiliation>Caspary Research Institute of The Animal Medical Center, New York, NY, USA</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Alexander L</forename><surname>Magnon</surname></persName><roleName type="degree">BS</roleName><affiliation>Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA</affiliation></author><author xml:id="author-0003"><persName><forename type="first">Si-Kwang</forename><surname>Liu</surname></persName><roleName type="degree">DVM, PhD</roleName><affiliation>Caspary Research Institute of The Animal Medical Center, New York, NY, USA</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Jeffrey A</forename><surname>Towbin</surname></persName><roleName type="degree">MD</roleName><affiliation>Baylor College of Medicine, Houston, TX, USA</affiliation></author><idno type="istex">69891B542A0AF2EC24913E6EECB25679F37BBAD2</idno><idno type="ark">ark:/67375/6H6-Q76BDC1K-2</idno><idno type="DOI">10.1016/S1054-8807(00)00031-4</idno><idno type="PII">S1054-8807(00)00031-4</idno></analytic><monogr><title level="j">Cardiovascular Pathology</title><title level="j" type="abbrev">CVP</title><idno type="pISSN">1054-8807</idno><idno type="PII">S1054-8807(00)X0030-0</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="2000"></date><biblScope unit="volume">9</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="119">119</biblScope><biblScope unit="page" to="126">126</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2000</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: Viral myocarditis has been suggested as an etiology for cardiomyopathy in several mammalian species. Myocarditis and idiopathic cardiomyopathy have been reported in the domestic cat, although a viral etiology has not been demonstrated. Because of the continuing interest in the potential relationship between viral myocarditis and cardiomyopathy, we evaluated hearts from cats with spontaneous, idiopathic cardiomyopathy for viral genomic material within myocytes by polymerase chain reaction, and for the presence of myocarditis by light microscopy. Thirty-one (31) formalin-fixed hearts from domestic cats who died of idiopathic cardiomyopathy were randomly selected from pathology archives. Seventeen (17) formalin-fixed hearts from healthy cats were similarly selected as normal controls. The polymerase chain reaction (PCR) was used to evaluate myocardial tissue for the presence of viral genome from feline panleukopenia virus, herpes virus, calici virus, and corona virus. Hearts were examined using light microscopy for histologic evidence of myocarditis according to the Dallas criteria. Panleukopenia virus was identified by PCR in 10 of 31 cats with cardiomyopathy but in none of the controls. Neither cardiomyopathic or control cats tested positive by PCR for herpes virus, calici virus, and corona virus. Myocarditis was detected by histologic examination in 18 of 31 cardiomyopathic cats and in none of 17 control cats. Myocarditis and or feline panleukopenia virus genome was detected in felines with idiopathic hypertrophic, dilated, and restrictive cardiomyopathy, suggesting a possible role of viral infection and inflammation in the pathogenesis of cardiomyopathy in this species.</p></abstract></profileDesc><revisionDesc><change when="2000-02-17">Modified</change><change when="2000">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-Q76BDC1K-2/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: ce:floats; body; tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"><istex:entity SYSTEM="gr1" NDATA="IMAGE" name="gr1"></istex:entity><istex:entity SYSTEM="gr2a" NDATA="IMAGE" name="gr2a"></istex:entity><istex:entity SYSTEM="gr2b" NDATA="IMAGE" name="gr2b"></istex:entity></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>CVP</jid><aid>5184</aid><ce:pii>S1054-8807(00)00031-4</ce:pii><ce:doi>10.1016/S1054-8807(00)00031-4</ce:doi><ce:copyright type="full-transfer" year="2000">Elsevier Science Inc.</ce:copyright></item-info><head><ce:dochead><ce:textfn>Articles</ce:textfn></ce:dochead><ce:title>Molecular Screening by Polymerase Chain Reaction Detects Panleukopenia Virus DNA in Formalin-Fixed Hearts from Cats with Idiopathic Cardiomyopathy and Myocarditis</ce:title><ce:author-group><ce:author><ce:given-name>Kathryn M</ce:given-name><ce:surname>Meurs</ce:surname><ce:degrees>DVM, PhD</ce:degrees><ce:cross-ref refid="AFF1">*</ce:cross-ref><ce:cross-ref refid="CORR1">*</ce:cross-ref><ce:e-address>meurs.1@osu.edu</ce:e-address></ce:author><ce:author><ce:given-name>Philip R</ce:given-name><ce:surname>Fox</ce:surname><ce:degrees>DVM</ce:degrees><ce:cross-ref refid="AFF2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Alexander L</ce:given-name><ce:surname>Magnon</ce:surname><ce:degrees>BS</ce:degrees><ce:cross-ref refid="AFF1">*</ce:cross-ref></ce:author><ce:author><ce:given-name>Si-Kwang</ce:given-name><ce:surname>Liu</ce:surname><ce:degrees>DVM, PhD</ce:degrees><ce:cross-ref refid="AFF2"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Jeffrey A</ce:given-name><ce:surname>Towbin</ce:surname><ce:degrees>MD</ce:degrees><ce:cross-ref refid="AFF3"><ce:sup>‡</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>*</ce:label><ce:textfn>Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>†</ce:label><ce:textfn>Caspary Research Institute of The Animal Medical Center, New York, NY, USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF3"><ce:label>‡</ce:label><ce:textfn>Baylor College of Medicine, Houston, TX, USA</ce:textfn></ce:affiliation><ce:correspondence id="CORR1"><ce:label>*</ce:label><ce:text>Address for correspondence: Dr. Kathryn M. Meurs, Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, 610 Vernon L. Tharp Street, Columbus, Ohio 43210, USA. Tel: 614-292-3551; Fax: 614-292-0895</ce:text></ce:correspondence></ce:author-group><ce:date-received day="3" month="1" year="2000"></ce:date-received><ce:date-revised day="17" month="2" year="2000"></ce:date-revised><ce:date-accepted day="8" month="3" year="2000"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>Viral myocarditis has been suggested as an etiology for cardiomyopathy in several mammalian species. Myocarditis and idiopathic cardiomyopathy have been reported in the domestic cat, although a viral etiology has not been demonstrated. Because of the continuing interest in the potential relationship between viral myocarditis and cardiomyopathy, we evaluated hearts from cats with spontaneous, idiopathic cardiomyopathy for viral genomic material within myocytes by polymerase chain reaction, and for the presence of myocarditis by light microscopy. Thirty-one <ce:cross-ref refid="BIB31">(31)</ce:cross-ref> formalin-fixed hearts from domestic cats who died of idiopathic cardiomyopathy were randomly selected from pathology archives. Seventeen <ce:cross-ref refid="BIB17">(17)</ce:cross-ref> formalin-fixed hearts from healthy cats were similarly selected as normal controls. The polymerase chain reaction (PCR) was used to evaluate myocardial tissue for the presence of viral genome from feline panleukopenia virus, herpes virus, calici virus, and corona virus. Hearts were examined using light microscopy for histologic evidence of myocarditis according to the Dallas criteria. Panleukopenia virus was identified by PCR in 10 of 31 cats with cardiomyopathy but in none of the controls. Neither cardiomyopathic or control cats tested positive by PCR for herpes virus, calici virus, and corona virus. Myocarditis was detected by histologic examination in 18 of 31 cardiomyopathic cats and in none of 17 control cats. Myocarditis and or feline panleukopenia virus genome was detected in felines with idiopathic hypertrophic, dilated, and restrictive cardiomyopathy, suggesting a possible role of viral infection and inflammation in the pathogenesis of cardiomyopathy in this species.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Molecular Screening by Polymerase Chain Reaction Detects Panleukopenia Virus DNA in Formalin-Fixed Hearts from Cats with Idiopathic Cardiomyopathy and Myocarditis</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Molecular Screening by Polymerase Chain Reaction Detects Panleukopenia Virus DNA in Formalin-Fixed Hearts from Cats with Idiopathic Cardiomyopathy and Myocarditis</title></titleInfo><name type="personal"><namePart type="given">Kathryn M</namePart><namePart type="family">Meurs</namePart><namePart type="termsOfAddress">DVM, PhD</namePart><affiliation>Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA</affiliation><affiliation>Address for correspondence: Dr. Kathryn M. Meurs, Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, 610 Vernon L. Tharp Street, Columbus, Ohio 43210, USA. Tel: 614-292-3551; Fax: 614-292-0895</affiliation><affiliation>E-mail: meurs.1@osu.edu</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Philip R</namePart><namePart type="family">Fox</namePart><namePart type="termsOfAddress">DVM</namePart><affiliation>Caspary Research Institute of The Animal Medical Center, New York, NY, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Alexander L</namePart><namePart type="family">Magnon</namePart><namePart type="termsOfAddress">BS</namePart><affiliation>Department of Veterinary Clinical Sciences, The Ohio State University College of Veterinary Medicine, Columbus, OH, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Si-Kwang</namePart><namePart type="family">Liu</namePart><namePart type="termsOfAddress">DVM, PhD</namePart><affiliation>Caspary Research Institute of The Animal Medical Center, New York, NY, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Jeffrey A</namePart><namePart type="family">Towbin</namePart><namePart type="termsOfAddress">MD</namePart><affiliation>Baylor College of Medicine, Houston, TX, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2000</dateIssued><dateModified encoding="w3cdtf">2000-02-17</dateModified><copyrightDate encoding="w3cdtf">2000</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: Viral myocarditis has been suggested as an etiology for cardiomyopathy in several mammalian species. Myocarditis and idiopathic cardiomyopathy have been reported in the domestic cat, although a viral etiology has not been demonstrated. Because of the continuing interest in the potential relationship between viral myocarditis and cardiomyopathy, we evaluated hearts from cats with spontaneous, idiopathic cardiomyopathy for viral genomic material within myocytes by polymerase chain reaction, and for the presence of myocarditis by light microscopy. Thirty-one (31) formalin-fixed hearts from domestic cats who died of idiopathic cardiomyopathy were randomly selected from pathology archives. Seventeen (17) formalin-fixed hearts from healthy cats were similarly selected as normal controls. The polymerase chain reaction (PCR) was used to evaluate myocardial tissue for the presence of viral genome from feline panleukopenia virus, herpes virus, calici virus, and corona virus. Hearts were examined using light microscopy for histologic evidence of myocarditis according to the Dallas criteria. Panleukopenia virus was identified by PCR in 10 of 31 cats with cardiomyopathy but in none of the controls. Neither cardiomyopathic or control cats tested positive by PCR for herpes virus, calici virus, and corona virus. Myocarditis was detected by histologic examination in 18 of 31 cardiomyopathic cats and in none of 17 control cats. Myocarditis and or feline panleukopenia virus genome was detected in felines with idiopathic hypertrophic, dilated, and restrictive cardiomyopathy, suggesting a possible role of viral infection and inflammation in the pathogenesis of cardiomyopathy in this species.</abstract><note type="content">Section title: Articles</note><note type="content">Figure 1: Agarose gel electrophoresis of the 397 base pair region of the nonstructural protein 1 of panleukopenia. Lanes 1, 2, 4, 7, 8 contain negative samples from cardiomyopathic cats. Lane 11 is a negative control, lane 12 is a positive control. The initial lane (identified by an asterisk) contains the DNA ladder</note><note type="content">Figure 2: Photomicrographs of representative myocardial sections from cats with cardiomyopathy and myocarditis. (Top) Section from the left ventricular free wall. Extensive mixed inflammatory infiltrate is located predominantly in the subendocardial region consisting of lymphocytes, neutrophils, and plasmacytes. Adjacent myocyte necrosis is severe. Hematoxylin and eosin stain (H&E); magnification ×200. (Bottom) Section from the right auricle. Diffuse neutrophilic infiltrate is associated with extensive myocardial necrosis which is particularly prominent in the subendocardial region. Subendocardial hemorrhage is present. Hematoxylin and eosin stain (H&E); magnification ×100</note><note type="content">Table 1: Polymerase Chain Reaction Conditions for Viral Amplification</note><relatedItem type="host"><titleInfo><title>Cardiovascular Pathology</title></titleInfo><titleInfo type="abbreviated"><title>CVP</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">2000</dateIssued></originInfo><identifier type="ISSN">1054-8807</identifier><identifier type="PII">S1054-8807(00)X0030-0</identifier><part><date>2000</date><detail type="volume"><number>9</number><caption>vol.</caption></detail><detail type="issue"><number>2</number><caption>no.</caption></detail><extent unit="issue-pages"><start>67</start><end>136</end></extent><extent unit="pages"><start>119</start><end>126</end></extent></part></relatedItem><identifier type="istex">69891B542A0AF2EC24913E6EECB25679F37BBAD2</identifier><identifier type="ark">ark:/67375/6H6-Q76BDC1K-2</identifier><identifier type="DOI">10.1016/S1054-8807(00)00031-4</identifier><identifier type="PII">S1054-8807(00)00031-4</identifier><accessCondition type="use and reproduction" contentType="copyright">©2000 Elsevier Science Inc.</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource><recordOrigin>Elsevier Science Inc., ©2000</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-Q76BDC1K-2/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/CovidV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000055 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 000055 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= CovidV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:69891B542A0AF2EC24913E6EECB25679F37BBAD2
|texte= Molecular Screening by Polymerase Chain Reaction Detects Panleukopenia Virus DNA in Formalin-Fixed Hearts from Cats with Idiopathic Cardiomyopathy and Myocarditis
}}
| This area was generated with Dilib version V0.6.33. |  |