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Demonstration of Feline corona virus (FCV) antigen in organs of cats suspected of feline infectious peritonitis (FIP) disease

Identifieur interne : 000052 ( Istex/Corpus ); précédent : 000051; suivant : 000053

Demonstration of Feline corona virus (FCV) antigen in organs of cats suspected of feline infectious peritonitis (FIP) disease

Auteurs : Kristina Hök

Source :

RBID : ISTEX:1420667F53CAD49182CF1B505CE84F2EA8F60B94

Abstract

Cryosections of organs and smears from membrana nictitans from cats suspected of having spontaneous infection with feline infectious peritonitis virus (FIPV), were investigated using an indirect immunofluorescence assay (UFA) in order to detect the presence of feline corona virus (FCV). In 113 cats, from each of which six organs were screened, virus antigen was found most often in membrana nictitans and lung. Out of these animals an additional six organs from a group of 30 cats were screened. In these cats membrana nictitans, parotid gland, thymus and apex of caecum had the highest incidence of virus antigen (90%). The lowest incidence of virus antigen was found in the spleen (60%). There was a clear demonstration of a higher incidence of antigen present in more than half of the total number of screened organs per cat (P < 0.0005). No statistical difference was observed between sexes when comparing the incidence of virus antigen in different organs. Virus antigen was present in less organs in cats with no lesions suggestive of FIP disease compared to cats with such lesions (P < 0.001). A similar distribution of the incidence of FCV antigen in the investigated organs was observed in these two groups.

Url:
DOI: 10.1111/j.1699-0463.1990.tb04985.x

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<unparsedEditorialHistory>Received November 6, 1989. Accepted January 23, 1990.</unparsedEditorialHistory>
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<title type="main">Demonstration of Feline corona virus (FCV) antigen in organs of cats suspected of feline infectious peritonitis (FIP) disease</title>
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<givenNames>KRISTINA</givenNames>
<familyName>HÖK</familyName>
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<unparsedAffiliation>Depart. of Medical Microbial Ecology and The Laboratory Animal Unit, Karolinska Institute, Stockholm, and Dept. of Pathology, The National Veterinary Institute, Uppsala, Sweden</unparsedAffiliation>
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<keywordGroup xml:lang="en">
<keyword xml:id="k1">Feline infectious peritonitis</keyword>
<keyword xml:id="k2">FIP</keyword>
<keyword xml:id="k3">
<i>membrana nictitans</i>
</keyword>
<keyword xml:id="k4">coronavirus</keyword>
<keyword xml:id="k5">indirect immunofluorescence assay (UFA)</keyword>
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<p>Cryosections of organs and smears from
<i>membrana nictitans</i>
from cats suspected of having spontaneous infection with feline infectious peritonitis virus (FIPV), were investigated using an indirect immunofluorescence assay (UFA) in order to detect the presence of feline corona virus (FCV). In 113 cats, from each of which six organs were screened, virus antigen was found most often in
<i>membrana nictitans</i>
and lung. Out of these animals an additional six organs from a group of 30 cats were screened. In these cats
<i>membrana nictitans</i>
, parotid gland, thymus and apex of caecum had the highest incidence of virus antigen (90%). The lowest incidence of virus antigen was found in the spleen (60%). There was a clear demonstration of a higher incidence of antigen present in more than half of the total number of screened organs per cat (P < 0.0005). No statistical difference was observed between sexes when comparing the incidence of virus antigen in different organs. Virus antigen was present in less organs in cats with no lesions suggestive of FIP disease compared to cats with such lesions (P < 0.001). A similar distribution of the incidence of FCV antigen in the investigated organs was observed in these two groups.</p>
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<title>Demonstration of Feline corona virus (FCV) antigen in organs of cats suspected of feline infectious peritonitis (FIP) disease</title>
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<title>Demonstration of Feline corona virus (FCV) antigen in organs of cats suspected of feline infectious peritonitis (FIP) disease</title>
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<name type="personal">
<namePart type="given">KRISTINA</namePart>
<namePart type="family">HÖK</namePart>
<affiliation>Depart. of Medical Microbial Ecology and The Laboratory Animal Unit, Karolinska Institute, Stockholm, and Dept. of Pathology, The National Veterinary Institute, Uppsala, Sweden</affiliation>
<affiliation>Correspondence address: Dept. of Medical Microbial Ecology, Karolinska Institutet, Box 60 400, S‐10401 Stockholm, Sweden.</affiliation>
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<publisher>Blackwell Publishing Ltd</publisher>
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<dateIssued encoding="w3cdtf">1990-07</dateIssued>
<edition>Received November 6, 1989. Accepted January 23, 1990.</edition>
<copyrightDate encoding="w3cdtf">1990</copyrightDate>
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<abstract lang="en">Cryosections of organs and smears from membrana nictitans from cats suspected of having spontaneous infection with feline infectious peritonitis virus (FIPV), were investigated using an indirect immunofluorescence assay (UFA) in order to detect the presence of feline corona virus (FCV). In 113 cats, from each of which six organs were screened, virus antigen was found most often in membrana nictitans and lung. Out of these animals an additional six organs from a group of 30 cats were screened. In these cats membrana nictitans, parotid gland, thymus and apex of caecum had the highest incidence of virus antigen (90%). The lowest incidence of virus antigen was found in the spleen (60%). There was a clear demonstration of a higher incidence of antigen present in more than half of the total number of screened organs per cat (P < 0.0005). No statistical difference was observed between sexes when comparing the incidence of virus antigen in different organs. Virus antigen was present in less organs in cats with no lesions suggestive of FIP disease compared to cats with such lesions (P < 0.001). A similar distribution of the incidence of FCV antigen in the investigated organs was observed in these two groups.</abstract>
<subject lang="en">
<genre>keywords</genre>
<topic>Feline infectious peritonitis</topic>
<topic>FIP</topic>
<topic>membrana nictitans</topic>
<topic>coronavirus</topic>
<topic>indirect immunofluorescence assay (UFA)</topic>
</subject>
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<identifier type="ISSN">0903-4641</identifier>
<identifier type="eISSN">1600-0463</identifier>
<identifier type="DOI">10.1111/(ISSN)1600-0463</identifier>
<identifier type="PublisherID">APM</identifier>
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<number>7‐12</number>
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