Clinical implications of different specimen types for nucleic acid testing in two cases of COVID-19.
Identifieur interne : 000503 ( Main/Corpus ); précédent : 000502; suivant : 000504Clinical implications of different specimen types for nucleic acid testing in two cases of COVID-19.
Auteurs : Hengwei Fan ; Xiaqing Yu ; Xiaohui Fu ; Hengmei Zhu ; Zhongwei Lv ; Wanwan Yi ; Qian ZhangSource :
- The Journal of international medical research [ 1473-2300 ] ; 2020.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : RNA, Viral.
- diagnosis : Coronavirus Infections, Pneumonia, Viral.
- genetics : Betacoronavirus.
- methods : Molecular Diagnostic Techniques.
- Aged, Humans, Male, Middle Aged, Pandemics.
Abstract
The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid test is currently the gold standard for diagnosing coronavirus disease 2019 (COVID-19). This disease requires high-quality viral nucleic acid tests, and selecting the type of specimen from patients, who are at different disease stages, to use in the nucleic acid test is challenging. This article reports in detail the diagnosis and treatment process for two patients with confirmed COVID-19 and analyzes the results of the SARS-CoV-2 nucleic acid tests that were used for different types of specimens (sputum from deep cough, nasopharyngeal swab, and feces). The nucleic acid testing results of sputum from deep cough showed the best performance for positive detection. Our findings provide a reference for selecting the most suitable specimen for the clinical diagnosis of COVID-19 and improving the positive detection rate.
DOI: 10.1177/0300060520949067
PubMed: 32840148
PubMed Central: PMC7447863
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pubmed:32840148Le document en format XML
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China.</nlm:affiliation></affiliation></author><author><name sortKey="Fu, Xiaohui" sort="Fu, Xiaohui" uniqKey="Fu X" first="Xiaohui" last="Fu">Xiaohui Fu</name><affiliation><nlm:affiliation>Department of Infection medicine, the Guanggu District of Hubei Province Maternal and Child Health Hospital, Wuhan, Hubei, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Biliary, the Eastern Hepatobiliary Surgery Hospital, Navy Medical University (Second Military Medical University), Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhu, Hengmei" sort="Zhu, Hengmei" uniqKey="Zhu H" first="Hengmei" last="Zhu">Hengmei Zhu</name><affiliation><nlm:affiliation>Department of Infection medicine, the Guanggu District of Hubei Province Maternal and Child Health Hospital, Wuhan, Hubei, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Special Needs, the Eastern Hepatobiliary Surgery Hospital, Navy Medical University (Second Military Medical University), Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Lv, Zhongwei" sort="Lv, Zhongwei" uniqKey="Lv Z" first="Zhongwei" last="Lv">Zhongwei Lv</name><affiliation><nlm:affiliation>Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yi, Wanwan" sort="Yi, Wanwan" uniqKey="Yi W" first="Wanwan" last="Yi">Wanwan Yi</name><affiliation><nlm:affiliation>Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Qian" sort="Zhang, Qian" uniqKey="Zhang Q" first="Qian" last="Zhang">Qian Zhang</name><affiliation><nlm:affiliation>Department of Infection medicine, the Guanggu District of Hubei Province Maternal and Child Health Hospital, Wuhan, Hubei, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Biotherapy, the Eastern Hepatobiliary Surgery Hospital, Navy Medical University (Second Military Medical University), Shanghai, China.</nlm:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2020">2020</date><idno type="RBID">pubmed:32840148</idno><idno type="pmid">32840148</idno><idno type="doi">10.1177/0300060520949067</idno><idno type="pmc">PMC7447863</idno><idno type="wicri:Area/Main/Corpus">000503</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000503</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Clinical implications of different specimen types for nucleic acid testing in two cases of COVID-19.</title><author><name sortKey="Fan, Hengwei" sort="Fan, Hengwei" uniqKey="Fan H" first="Hengwei" last="Fan">Hengwei Fan</name><affiliation><nlm:affiliation>Department of Hepatic Surgery, the Eastern Hepatobiliary Surgery Hospital, Navy Medical University (Second Military Medical University), Shanghai, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Infection medicine, the Guanggu District of Hubei Province Maternal and Child Health Hospital, Wuhan, Hubei, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yu, Xiaqing" sort="Yu, Xiaqing" uniqKey="Yu X" first="Xiaqing" last="Yu">Xiaqing Yu</name><affiliation><nlm:affiliation>Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Fu, Xiaohui" sort="Fu, Xiaohui" uniqKey="Fu X" first="Xiaohui" last="Fu">Xiaohui Fu</name><affiliation><nlm:affiliation>Department of Infection medicine, the Guanggu District of Hubei Province Maternal and Child Health Hospital, Wuhan, Hubei, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Biliary, the Eastern Hepatobiliary Surgery Hospital, Navy Medical University (Second Military Medical University), Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhu, Hengmei" sort="Zhu, Hengmei" uniqKey="Zhu H" first="Hengmei" last="Zhu">Hengmei Zhu</name><affiliation><nlm:affiliation>Department of Infection medicine, the Guanggu District of Hubei Province Maternal and Child Health Hospital, Wuhan, Hubei, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Special Needs, the Eastern Hepatobiliary Surgery Hospital, Navy Medical University (Second Military Medical University), Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Lv, Zhongwei" sort="Lv, Zhongwei" uniqKey="Lv Z" first="Zhongwei" last="Lv">Zhongwei Lv</name><affiliation><nlm:affiliation>Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Yi, Wanwan" sort="Yi, Wanwan" uniqKey="Yi W" first="Wanwan" last="Yi">Wanwan Yi</name><affiliation><nlm:affiliation>Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.</nlm:affiliation></affiliation></author><author><name sortKey="Zhang, Qian" sort="Zhang, Qian" uniqKey="Zhang Q" first="Qian" last="Zhang">Qian Zhang</name><affiliation><nlm:affiliation>Department of Infection medicine, the Guanggu District of Hubei Province Maternal and Child Health Hospital, Wuhan, Hubei, China.</nlm:affiliation></affiliation><affiliation><nlm:affiliation>Department of Biotherapy, the Eastern Hepatobiliary Surgery Hospital, Navy Medical University (Second Military Medical University), Shanghai, China.</nlm:affiliation></affiliation></author></analytic><series><title level="j">The Journal of international medical research</title><idno type="eISSN">1473-2300</idno><imprint><date when="2020" type="published">2020</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aged (MeSH)</term><term>Betacoronavirus (genetics)</term><term>Coronavirus Infections (diagnosis)</term><term>Humans (MeSH)</term><term>Male (MeSH)</term><term>Middle Aged (MeSH)</term><term>Molecular Diagnostic Techniques (methods)</term><term>Pandemics (MeSH)</term><term>Pneumonia, Viral (diagnosis)</term><term>RNA, Viral (analysis)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term><term>Pneumonia, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Betacoronavirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Molecular Diagnostic Techniques</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Aged</term><term>Humans</term><term>Male</term><term>Middle Aged</term><term>Pandemics</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid test is currently the gold standard for diagnosing coronavirus disease 2019 (COVID-19). This disease requires high-quality viral nucleic acid tests, and selecting the type of specimen from patients, who are at different disease stages, to use in the nucleic acid test is challenging. This article reports in detail the diagnosis and treatment process for two patients with confirmed COVID-19 and analyzes the results of the SARS-CoV-2 nucleic acid tests that were used for different types of specimens (sputum from deep cough, nasopharyngeal swab, and feces). The nucleic acid testing results of sputum from deep cough showed the best performance for positive detection. Our findings provide a reference for selecting the most suitable specimen for the clinical diagnosis of COVID-19 and improving the positive detection rate.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32840148</PMID><DateCompleted><Year>2020</Year><Month>08</Month><Day>31</Day></DateCompleted><DateRevised><Year>2020</Year><Month>08</Month><Day>31</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1473-2300</ISSN><JournalIssue CitedMedium="Internet"><Volume>48</Volume><Issue>8</Issue><PubDate><Year>2020</Year><Month>Aug</Month></PubDate></JournalIssue><Title>The Journal of international medical research</Title><ISOAbbreviation>J Int Med Res</ISOAbbreviation></Journal><ArticleTitle>Clinical implications of different specimen types for nucleic acid testing in two cases of COVID-19.</ArticleTitle><Pagination><MedlinePgn>300060520949067</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1177/0300060520949067</ELocationID><Abstract><AbstractText>The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleic acid test is currently the gold standard for diagnosing coronavirus disease 2019 (COVID-19). This disease requires high-quality viral nucleic acid tests, and selecting the type of specimen from patients, who are at different disease stages, to use in the nucleic acid test is challenging. This article reports in detail the diagnosis and treatment process for two patients with confirmed COVID-19 and analyzes the results of the SARS-CoV-2 nucleic acid tests that were used for different types of specimens (sputum from deep cough, nasopharyngeal swab, and feces). The nucleic acid testing results of sputum from deep cough showed the best performance for positive detection. Our findings provide a reference for selecting the most suitable specimen for the clinical diagnosis of COVID-19 and improving the positive detection rate.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Fan</LastName><ForeName>Hengwei</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Hepatic Surgery, the Eastern Hepatobiliary Surgery Hospital, Navy Medical University (Second Military Medical University), Shanghai, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Infection medicine, the Guanggu District of Hubei Province Maternal and Child Health Hospital, Wuhan, Hubei, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yu</LastName><ForeName>Xiaqing</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Fu</LastName><ForeName>Xiaohui</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Infection medicine, the Guanggu District of Hubei Province Maternal and Child Health Hospital, Wuhan, Hubei, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Biliary, the Eastern Hepatobiliary Surgery Hospital, Navy Medical University (Second Military Medical University), Shanghai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Hengmei</ForeName><Initials>H</Initials><AffiliationInfo><Affiliation>Department of Infection medicine, the Guanggu District of Hubei Province Maternal and Child Health Hospital, Wuhan, Hubei, China.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Department of Special Needs, the Eastern Hepatobiliary Surgery Hospital, Navy Medical University (Second Military Medical University), Shanghai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lv</LastName><ForeName>Zhongwei</ForeName><Initials>Z</Initials><Identifier Source="ORCID">https://orcid.org/0000-0003-3370-5560</Identifier><AffiliationInfo><Affiliation>Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yi</LastName><ForeName>Wanwan</ForeName><Initials>W</Initials><AffiliationInfo><Affiliation>Department of Nuclear Medicine, Shanghai Tenth People's Hospital, Tongji University School of Medicine, Shanghai, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Qian</ForeName><Initials>Q</Initials><AffiliationInfo><Affiliation>Department of Infection medicine, the Guanggu District of Hubei Province Maternal and Child Health Hospital, Wuhan, Hubei, 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Mar;579(7798):265-269</Citation><ArticleIdList><ArticleId IdType="pubmed">32015508</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2020 May;581(7809):465-469</Citation><ArticleIdList><ArticleId IdType="pubmed">32235945</ArticleId></ArticleIdList></Reference><Reference><Citation>AJR Am J Roentgenol. 2020 Jul;215(1):121-126</Citation><ArticleIdList><ArticleId IdType="pubmed">32174128</ArticleId></ArticleIdList></Reference><Reference><Citation>J Clin Microbiol. 2020 May 26;58(6):</Citation><ArticleIdList><ArticleId IdType="pubmed">32245835</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Chem Lab Med. 2020 Jun 25;58(7):1089-1094</Citation><ArticleIdList><ArticleId IdType="pubmed">32301745</ArticleId></ArticleIdList></Reference><Reference><Citation>JAMA. 2020 Mar 11;:</Citation><ArticleIdList><ArticleId IdType="pubmed">32159775</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed></record>Pour manipuler ce document sous Unix 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