Inhibition of human coronavirus 229E infection in human epithelial lung cells (L132) by chloroquine: involvement of p38 MAPK and ERK.
Identifieur interne : 000108 ( Ncbi/Checkpoint ); précédent : 000107; suivant : 000109Inhibition of human coronavirus 229E infection in human epithelial lung cells (L132) by chloroquine: involvement of p38 MAPK and ERK.
Auteurs : Masakazu Kono [Japon] ; Koichiro Tatsumi ; Alberto M. Imai ; Kengo Saito ; Takayuki Kuriyama ; Hiroshi ShirasawaSource :
- Antiviral research [ 0166-3542 ] ; 2008.
Descripteurs français
- KwdFr :
- Antiviraux (pharmacologie), Cellules épithéliales (virologie), Chloroquine (pharmacologie), Coronavirus humain 229E (), Effet cytopathogène viral (), Extracellular Signal-Regulated MAP Kinases (antagonistes et inhibiteurs), Humains, Imidazoles (pharmacologie), Lignée cellulaire, Poumon (cytologie), Poumon (virologie), Pyridines (pharmacologie), RT-PCR, Réplication virale (), p38 Mitogen-Activated Protein Kinases (antagonistes et inhibiteurs).
- MESH :
- antagonistes et inhibiteurs : Extracellular Signal-Regulated MAP Kinases, p38 Mitogen-Activated Protein Kinases.
- cytologie : Poumon.
- pharmacologie : Antiviraux, Chloroquine, Imidazoles, Pyridines.
- virologie : Cellules épithéliales, Poumon.
- Coronavirus humain 229E, Effet cytopathogène viral, Humains, Lignée cellulaire, RT-PCR, Réplication virale.
English descriptors
- KwdEn :
- Antiviral Agents (pharmacology), Cell Line, Chloroquine (pharmacology), Coronavirus 229E, Human (drug effects), Cytopathogenic Effect, Viral (drug effects), Epithelial Cells (virology), Extracellular Signal-Regulated MAP Kinases (antagonists & inhibitors), Humans, Imidazoles (pharmacology), Lung (cytology), Lung (virology), Pyridines (pharmacology), Reverse Transcriptase Polymerase Chain Reaction, Virus Replication (drug effects), p38 Mitogen-Activated Protein Kinases (antagonists & inhibitors).
- MESH :
- chemical , antagonists & inhibitors : Extracellular Signal-Regulated MAP Kinases, p38 Mitogen-Activated Protein Kinases.
- chemical , pharmacology : Antiviral Agents, Chloroquine, Imidazoles, Pyridines.
- cytology : Lung.
- drug effects : Coronavirus 229E, Human, Cytopathogenic Effect, Viral, Virus Replication.
- virology : Epithelial Cells, Lung.
- Cell Line, Humans, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
The antiviral effects of chloroquine (CQ) on human coronavirus 229E (HCoV-229E) infection of human fetal lung cell line, L132 are reported. CQ significantly decreased the viral replication at concentrations lower than in clinical usage. We demonstrated that CQ affects the activation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK). Furthermore, p38 MAPK inhibitor, SB203580, inhibits CPE induced by HCoV-229E infection and viral replication. Our findings suggest that CQ affects the activation of MAPKs, involved in the replication of HCoV-229E.
DOI: 10.1016/j.antiviral.2007.10.011
PubMed: 18055026
Affiliations:
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pubmed:18055026Le document en format XML
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<term>Cytopathogenic Effect, Viral (drug effects)</term>
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<term>Chloroquine (pharmacologie)</term>
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<term>Effet cytopathogène viral ()</term>
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<term>Pyridines</term>
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<front><div type="abstract" xml:lang="en">The antiviral effects of chloroquine (CQ) on human coronavirus 229E (HCoV-229E) infection of human fetal lung cell line, L132 are reported. CQ significantly decreased the viral replication at concentrations lower than in clinical usage. We demonstrated that CQ affects the activation of p38 mitogen-activated protein kinase (MAPK) and extracellular signal-regulated kinase (ERK). Furthermore, p38 MAPK inhibitor, SB203580, inhibits CPE induced by HCoV-229E infection and viral replication. Our findings suggest that CQ affects the activation of MAPKs, involved in the replication of HCoV-229E.</div>
</front>
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<tree><noCountry><name sortKey="Imai, Alberto M" sort="Imai, Alberto M" uniqKey="Imai A" first="Alberto M" last="Imai">Alberto M. Imai</name>
<name sortKey="Kuriyama, Takayuki" sort="Kuriyama, Takayuki" uniqKey="Kuriyama T" first="Takayuki" last="Kuriyama">Takayuki Kuriyama</name>
<name sortKey="Saito, Kengo" sort="Saito, Kengo" uniqKey="Saito K" first="Kengo" last="Saito">Kengo Saito</name>
<name sortKey="Shirasawa, Hiroshi" sort="Shirasawa, Hiroshi" uniqKey="Shirasawa H" first="Hiroshi" last="Shirasawa">Hiroshi Shirasawa</name>
<name sortKey="Tatsumi, Koichiro" sort="Tatsumi, Koichiro" uniqKey="Tatsumi K" first="Koichiro" last="Tatsumi">Koichiro Tatsumi</name>
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<country name="Japon"><noRegion><name sortKey="Kono, Masakazu" sort="Kono, Masakazu" uniqKey="Kono M" first="Masakazu" last="Kono">Masakazu Kono</name>
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