ChloroquineV1, Main, Merge, bibRecord, 001E12

Chloroquine normalizes aberrant transforming growth factor beta activity in cystic fibrosis bronchial epithelial cells

Identifieur interne : 001E12 ( Main/Merge ); précédent : 001E11; suivant : 001E13

Chloroquine normalizes aberrant transforming growth factor beta activity in cystic fibrosis bronchial epithelial cells

Auteurs : Elizabeth A. Perkett [États-Unis] ; Wojclech Ornatowski [États-Unis] ; Jens F. Poschet [États-Unis] ; Vojo Deretic [États-Unis]

Source :

Pediatric pulmonology [ 8755-6863 ] ; 2006.

RBID : Pascal:06-0360316

Descripteurs français

Pascal (Inist)
- Mucoviscidose, Appareil respiratoire pathologie, Chloroquine, Anomalie, Facteur croissance transformant β, Activité, Bronche, Cellule épithéliale, Poumon, Fibrose, Chronique, Inflammation, Furin, Appareil Golgi, Pédiatrie, Antipaludique, Antiparasitaire.
Wicri :
- topic : Pédiatrie.

English descriptors

KwdEn :
- Activity, Anomaly, Antimalarial, Bronchus, Chloroquine, Chronic, Cystic fibrosis, Epithelial cell, Fibrosis, Furin, Golgi apparatus, Inflammation, Lung, Parasiticide, Pediatrics, Respiratory disease, Transforming growth factor β.

Abstract

Cystic fibrosis (CF) remains a fatal progressive disease in spite of the discovery and characterization of the CFTR gene. Transforming growth factor beta (TGF-p) has been implicated in pathophysiology of CF Previous reports have shown the trans-Golgi network (TGN) is hyperacdified in CF epithelial cells in culture and that this hyperacidification can be corrected with the membrane permeant weak base, chloroquine. In this study bioactive TGF-p produced by CF and normal cells was measured using a reporter cell line with a TGF-p responsive promoter linked to luciferase. Increased levels of TGF-β were detected in the conditioned media from CF epithelial cells compared to their matched controls-(IB3-1 vs. S9; pCEP-R vs. pCEP, CuFi-4 vs. NuLi-1). Levels of TGF-β were normalized with chloroquine indicating that the hyperacidification of the TGN of CF cells is responsible for the altered TGF-p levels.

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Pascal:06-0360316

Le document en format XML

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<author><name sortKey="Ornatowski, Wojclech" sort="Ornatowski, Wojclech" uniqKey="Ornatowski W" first="Wojclech" last="Ornatowski">Wojclech Ornatowski</name>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Activity</term>
<term>Anomaly</term>
<term>Antimalarial</term>
<term>Bronchus</term>
<term>Chloroquine</term>
<term>Chronic</term>
<term>Cystic fibrosis</term>
<term>Epithelial cell</term>
<term>Fibrosis</term>
<term>Furin</term>
<term>Golgi apparatus</term>
<term>Inflammation</term>
<term>Lung</term>
<term>Parasiticide</term>
<term>Pediatrics</term>
<term>Respiratory disease</term>
<term>Transforming growth factor β</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Mucoviscidose</term>
<term>Appareil respiratoire pathologie</term>
<term>Chloroquine</term>
<term>Anomalie</term>
<term>Facteur croissance transformant β</term>
<term>Activité</term>
<term>Bronche</term>
<term>Cellule épithéliale</term>
<term>Poumon</term>
<term>Fibrose</term>
<term>Chronique</term>
<term>Inflammation</term>
<term>Furin</term>
<term>Appareil Golgi</term>
<term>Pédiatrie</term>
<term>Antipaludique</term>
<term>Antiparasitaire</term>
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<front><div type="abstract" xml:lang="en">Cystic fibrosis (CF) remains a fatal progressive disease in spite of the discovery and characterization of the CFTR gene. Transforming growth factor beta (TGF-p) has been implicated in pathophysiology of CF Previous reports have shown the trans-Golgi network (TGN) is hyperacdified in CF epithelial cells in culture and that this hyperacidification can be corrected with the membrane permeant weak base, chloroquine. In this study bioactive TGF-p produced by CF and normal cells was measured using a reporter cell line with a TGF-p responsive promoter linked to luciferase. Increased levels of TGF-β were detected in the conditioned media from CF epithelial cells compared to their matched controls-(IB3-1 vs. S9; pCEP-R vs. pCEP, CuFi-4 vs. NuLi-1). Levels of TGF-β were normalized with chloroquine indicating that the hyperacidification of the TGN of CF cells is responsible for the altered TGF-p levels.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
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<tree><country name="États-Unis"><region name="Nouveau-Mexique"><name sortKey="Perkett, Elizabeth A" sort="Perkett, Elizabeth A" uniqKey="Perkett E" first="Elizabeth A." last="Perkett">Elizabeth A. Perkett</name>
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<name sortKey="Deretic, Vojo" sort="Deretic, Vojo" uniqKey="Deretic V" first="Vojo" last="Deretic">Vojo Deretic</name>
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<name sortKey="Ornatowski, Wojclech" sort="Ornatowski, Wojclech" uniqKey="Ornatowski W" first="Wojclech" last="Ornatowski">Wojclech Ornatowski</name>
<name sortKey="Poschet, Jens F" sort="Poschet, Jens F" uniqKey="Poschet J" first="Jens F." last="Poschet">Jens F. Poschet</name>
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Chloroquine normalizes aberrant transforming growth factor beta activity in cystic fibrosis bronchial epithelial cells

Chloroquine normalizes aberrant transforming growth factor beta activity in cystic fibrosis bronchial epithelial cells

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri