Clathrin-dependent endocytosis of claudin-2 by DFYSP peptide causes lysosomal damage in lung adenocarcinoma A549 cells.
Identifieur interne : 000E95 ( Main/Exploration ); précédent : 000E94; suivant : 000E96Clathrin-dependent endocytosis of claudin-2 by DFYSP peptide causes lysosomal damage in lung adenocarcinoma A549 cells.
Auteurs : Akira Ikari [Japon] ; Saeko Taga [Japon] ; Ryo Watanabe [Japon] ; Tomonari Sato [Japon] ; Shun Shimobaba [Japon] ; Hiroyuki Sonoki [Japon] ; Satoshi Endo [Japon] ; Toshiyuki Matsunaga [Japon] ; Hideki Sakai [Japon] ; Masahiko Yamaguchi [Japon] ; Yasuhiro Yamazaki [Japon] ; Junko Sugatani [Japon]Source :
- Biochimica et biophysica acta [ 0006-3002 ] ; 2015.
Descripteurs français
- KwdFr :
- Apoptose (), Clathrine (métabolisme), Claudine-2 (métabolisme), Endocytose (), Humains, Lignée cellulaire tumorale, Lysosomes (), Lysosomes (anatomopathologie), Lysosomes (métabolisme), Peptides (administration et posologie), Relation dose-effet des médicaments, Tumeurs du poumon (anatomopathologie), Tumeurs du poumon (métabolisme).
- MESH :
- administration et posologie : Peptides.
- anatomopathologie : Lysosomes, Tumeurs du poumon.
- métabolisme : Clathrine, Claudine-2, Lysosomes, Tumeurs du poumon.
- Apoptose, Endocytose, Humains, Lignée cellulaire tumorale, Lysosomes, Relation dose-effet des médicaments.
English descriptors
- KwdEn :
- Apoptosis (drug effects), Cell Line, Tumor, Clathrin (metabolism), Claudin-2 (metabolism), Dose-Response Relationship, Drug, Endocytosis (drug effects), Humans, Lung Neoplasms (metabolism), Lung Neoplasms (pathology), Lysosomes (drug effects), Lysosomes (metabolism), Lysosomes (pathology), Peptides (administration & dosage).
- MESH :
- chemical , administration & dosage : Peptides.
- chemical , metabolism : Clathrin, Claudin-2.
- drug effects : Apoptosis, Endocytosis, Lysosomes.
- metabolism : Lung Neoplasms, Lysosomes.
- pathology : Lung Neoplasms, Lysosomes.
- Cell Line, Tumor, Dose-Response Relationship, Drug, Humans.
Abstract
Claudins are tight junctional proteins and comprise a family of over 20 members. Abnormal expression of claudins is reported to be involved in tumor progression. Claudin-2 is highly expressed in lung adenocarcinoma tissues and increases cell proliferation, whereas it is not expressed in normal tissues. Claudin-2-targeting molecules such as peptides and small molecules may be novel anti-cancer drugs. The short peptide with the sequence DFYSP, which mimics the second extracellular loop of claudin-2, decreased claudin-2 content in the cytoplasmic fraction of A549 cells. In contrast, it did not affect the content in the nuclear fraction. The decrease in claudin-2 content was inhibited by chloroquine (CQ), a lysosomal inhibitor, but not by MG-132, a proteasome inhibitor. In the presence of DFYSP peptide and CQ, claudin-2 was co-localized with LAMP-1, a lysosomal marker. The DFYSP peptide-induced decrease in claudin-2 content was inhibited by monodancylcadaverine (MDC), an inhibitor of clathrin-dependent endocytosis. DFYSP peptide increased lysosome content and cathepsin B release, and induced cellular injury, which were inhibited by MDC. Cellular injury induced by DFYSP peptide was inhibited by necrostatin-1, an inhibitor of necrotic cell death, but not by Z-VAD-FMK, an inhibitor of apoptotic cell death. Our data indicate that DFYSP peptide increases the accumulation of the peptide and claudin-2 into the lysosome, resulting in lysosomal damage. Claudin-2 may be a new target for lung cancer therapy.
DOI: 10.1016/j.bbamem.2015.07.003
PubMed: 26163137
Affiliations:
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Le document en format XML
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<author><name sortKey="Yamaguchi, Masahiko" sort="Yamaguchi, Masahiko" uniqKey="Yamaguchi M" first="Masahiko" last="Yamaguchi">Masahiko Yamaguchi</name>
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<term>Cell Line, Tumor</term>
<term>Clathrin (metabolism)</term>
<term>Claudin-2 (metabolism)</term>
<term>Dose-Response Relationship, Drug</term>
<term>Endocytosis (drug effects)</term>
<term>Humans</term>
<term>Lung Neoplasms (metabolism)</term>
<term>Lung Neoplasms (pathology)</term>
<term>Lysosomes (drug effects)</term>
<term>Lysosomes (metabolism)</term>
<term>Lysosomes (pathology)</term>
<term>Peptides (administration & dosage)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Apoptose ()</term>
<term>Clathrine (métabolisme)</term>
<term>Claudine-2 (métabolisme)</term>
<term>Endocytose ()</term>
<term>Humains</term>
<term>Lignée cellulaire tumorale</term>
<term>Lysosomes ()</term>
<term>Lysosomes (anatomopathologie)</term>
<term>Lysosomes (métabolisme)</term>
<term>Peptides (administration et posologie)</term>
<term>Relation dose-effet des médicaments</term>
<term>Tumeurs du poumon (anatomopathologie)</term>
<term>Tumeurs du poumon (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Peptides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Clathrin</term>
<term>Claudin-2</term>
</keywords>
<keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Peptides</term>
</keywords>
<keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Lysosomes</term>
<term>Tumeurs du poumon</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Apoptosis</term>
<term>Endocytosis</term>
<term>Lysosomes</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lung Neoplasms</term>
<term>Lysosomes</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Clathrine</term>
<term>Claudine-2</term>
<term>Lysosomes</term>
<term>Tumeurs du poumon</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Lung Neoplasms</term>
<term>Lysosomes</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cell Line, Tumor</term>
<term>Dose-Response Relationship, Drug</term>
<term>Humans</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Apoptose</term>
<term>Endocytose</term>
<term>Humains</term>
<term>Lignée cellulaire tumorale</term>
<term>Lysosomes</term>
<term>Relation dose-effet des médicaments</term>
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<front><div type="abstract" xml:lang="en">Claudins are tight junctional proteins and comprise a family of over 20 members. Abnormal expression of claudins is reported to be involved in tumor progression. Claudin-2 is highly expressed in lung adenocarcinoma tissues and increases cell proliferation, whereas it is not expressed in normal tissues. Claudin-2-targeting molecules such as peptides and small molecules may be novel anti-cancer drugs. The short peptide with the sequence DFYSP, which mimics the second extracellular loop of claudin-2, decreased claudin-2 content in the cytoplasmic fraction of A549 cells. In contrast, it did not affect the content in the nuclear fraction. The decrease in claudin-2 content was inhibited by chloroquine (CQ), a lysosomal inhibitor, but not by MG-132, a proteasome inhibitor. In the presence of DFYSP peptide and CQ, claudin-2 was co-localized with LAMP-1, a lysosomal marker. The DFYSP peptide-induced decrease in claudin-2 content was inhibited by monodancylcadaverine (MDC), an inhibitor of clathrin-dependent endocytosis. DFYSP peptide increased lysosome content and cathepsin B release, and induced cellular injury, which were inhibited by MDC. Cellular injury induced by DFYSP peptide was inhibited by necrostatin-1, an inhibitor of necrotic cell death, but not by Z-VAD-FMK, an inhibitor of apoptotic cell death. Our data indicate that DFYSP peptide increases the accumulation of the peptide and claudin-2 into the lysosome, resulting in lysosomal damage. Claudin-2 may be a new target for lung cancer therapy.</div>
</front>
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<name sortKey="Watanabe, Ryo" sort="Watanabe, Ryo" uniqKey="Watanabe R" first="Ryo" last="Watanabe">Ryo Watanabe</name>
<name sortKey="Yamaguchi, Masahiko" sort="Yamaguchi, Masahiko" uniqKey="Yamaguchi M" first="Masahiko" last="Yamaguchi">Masahiko Yamaguchi</name>
<name sortKey="Yamazaki, Yasuhiro" sort="Yamazaki, Yasuhiro" uniqKey="Yamazaki Y" first="Yasuhiro" last="Yamazaki">Yasuhiro Yamazaki</name>
</country>
</tree>
</affiliations>
</record>
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