ChloroquineV1, Main, Exploration, bibRecord, 003214

Comparisons of two in vitro cytotoxicity assays—The neutral red (NR) and tetrazolium MTT tests

Identifieur interne : 003214 ( Main/Exploration ); précédent : 003213; suivant : 003215

Comparisons of two in vitro cytotoxicity assays—The neutral red (NR) and tetrazolium MTT tests

Auteurs : E. Borenfreund [États-Unis] ; H. Babich [États-Unis] ; N. Martin-Alguacil [États-Unis]

Source :

Toxicology in Vitro [ 0887-2333 ] ; 1988.

RBID : ISTEX:B641C1CFF8BDAC57E8A4981719DF5ECEEE66EB6F

English descriptors

Teeft :
- Absorbance, Assay, Babich, Babich borenfreund, Borenfreund, Borenfreund puerner, Cancer chemotherapy, Chloroquine, Colorimetric, Cytotoxic, Cytotoxicity, Cytotoxicity assays, Cytotoxicity curves, Denizot lang, Lang, Leucovorin, Lower concentrations, Methotrexate, Midpoint, Midpoint cytotoxicity values, Mtts0, Mtts0 values, Nrs0, Puerner, Rapid colorimetric assay, Test agents, Tetrazolium, Tetrazolium salt, Toxicant, Toxicity.

Abstract

Abstract: The neutral red (NR) and tetrazolium MTT in vitro cytotoxicity assays were compared for 28 test agents of widely varying potency using the BALB/c mouse 3T3 fibroblast cell line as the bioindicator. For any given cell density in the microtitre plate well, the optical density absorbance with the NR assay was about twice that obtained with the MTT assay. However, there was good agreement (r = 0.939) between the ranking of the test agents on the basis of midpoint cytotoxicity values (NR50 and MTT50), although the assays were based on different physiological endpoints. Nevertheless, the two assays were found to differ in sensitivity for a few test agents, such as chloroquine sulphate and several antineoplastic drugs. Both assays were capable of demonstrating a reduction in the cytotoxicity of methotrexate by leucovorin.

Url:

https://api.istex.fr/ark:/67375/6H6-KBN92DB3-G/fulltext.pdf

DOI: 10.1016/0887-2333(88)90030-6

Affiliations:

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: The neutral red (NR) and tetrazolium MTT in vitro cytotoxicity assays were compared for 28 test agents of widely varying potency using the BALB/c mouse 3T3 fibroblast cell line as the bioindicator. For any given cell density in the microtitre plate well, the optical density absorbance with the NR assay was about twice that obtained with the MTT assay. However, there was good agreement (r = 0.939) between the ranking of the test agents on the basis of midpoint cytotoxicity values (NR50 and MTT50), although the assays were based on different physiological endpoints. Nevertheless, the two assays were found to differ in sensitivity for a few test agents, such as chloroquine sulphate and several antineoplastic drugs. Both assays were capable of demonstrating a reduction in the cytotoxicity of methotrexate by leucovorin.</div>
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Comparisons of two in vitro cytotoxicity assays—The neutral red (NR) and tetrazolium MTT tests

Comparisons of two in vitro cytotoxicity assays—The neutral red (NR) and tetrazolium MTT tests

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri