Starvation‐induced lysosomal degradation of aldolase B requires glutamine 111 in a signal sequence for chaperone‐mediated transport
Identifieur interne : 002316 ( Main/Exploration ); précédent : 002315; suivant : 002317Starvation‐induced lysosomal degradation of aldolase B requires glutamine 111 in a signal sequence for chaperone‐mediated transport
Auteurs : P. P. Susan [États-Unis] ; W. A. Dunn [États-Unis]Source :
- Journal of Cellular Physiology [ 0021-9541 ] ; 2001-04.
Abstract
Aldolase B is an abundant cytosolic protein found in all eukaryotic cells. Like many glycolytic enzymes, this protein was sequestered into lysosomes for degradation during nutrient starvation. We report here that the degradation of recombinant aldolase B was enhanced two‐fold when rat and human hepatoma cells were starved for amino acid and serum. In addition, starvation‐induced degradation of aldolase B was inhibited by chloroquine, an inhibitor of lysosomal proteinases and by 3‐methyladenine, an inhibitor of autophagy. Aldolase B has three lysosomal targeting motifs (Q12KKEL, Q58FREL, and IKLDQ111) that have been proposed to interact with hsc73 thereby initiating its transport into lysosomes. In this study, we have mutated the essential glutamine residues in each of these hsc73‐binding motifs in order to evaluate their roles in the lysosomal degradation of aldolase B during starvation. We have found that when glutamines 12 or 58 are mutated to asparagines enhanced degradation of aldolase B proceeded normally. However, when glutamine 111 was mutated to an asparagine or a threonine, starvation‐induced degradation was completely suppressed. These mutations did not appear to alter the tertiary structure of aldolase B since enzymatic activity was not affected. Our results suggest that starvation‐induced lysosomal degradation of aldolase B requires both autophagy and glutamine 111. We discuss the possible roles for autophagy and hsc73‐mediated transport in the lysosomal sequestration of aldolase B. © 2001 Wiley‐Liss, Inc.
Url:
DOI: 10.1002/1097-4652(2001)9999:9999<00::AID-JCP1050>3.0.CO;2-I
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000963
- to stream Istex, to step Curation: 000963
- to stream Istex, to step Checkpoint: 001142
- to stream Main, to step Merge: 002341
- to stream Main, to step Curation: 002316
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Starvation‐induced lysosomal degradation of aldolase B requires glutamine 111 in a signal sequence for chaperone‐mediated transport</title><author><name sortKey="Susan, P P" sort="Susan, P P" uniqKey="Susan P" first="P. P." last="Susan">P. P. Susan</name></author><author><name sortKey="Dunn, W A" sort="Dunn, W A" uniqKey="Dunn W" first="W. A." last="Dunn">W. A. Dunn</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:19753CA4DB6F5D17B3115B304D72899AA7965C1C</idno><date when="2001" year="2001">2001</date><idno type="doi">10.1002/1097-4652(2001)9999:9999<00::AID-JCP1050>3.0.CO;2-I</idno><idno type="url">https://api.istex.fr/ark:/67375/WNG-8MPQVP0L-F/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000963</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000963</idno><idno type="wicri:Area/Istex/Curation">000963</idno><idno type="wicri:Area/Istex/Checkpoint">001142</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">001142</idno><idno type="wicri:doubleKey">0021-9541:2001:Susan P:starvation:induced:lysosomal</idno><idno type="wicri:Area/Main/Merge">002341</idno><idno type="wicri:Area/Main/Curation">002316</idno><idno type="wicri:Area/Main/Exploration">002316</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main">Starvation‐induced lysosomal degradation of aldolase B requires glutamine 111 in a signal sequence for chaperone‐mediated transport</title><author><name sortKey="Susan, P P" sort="Susan, P P" uniqKey="Susan P" first="P. P." last="Susan">P. P. Susan</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville</wicri:cityArea></affiliation></author><author><name sortKey="Dunn, W A" sort="Dunn, W A" uniqKey="Dunn W" first="W. A." last="Dunn">W. A. Dunn</name><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Department of Anatomy and Cell Biology, University of Florida College of Medicine, Gainesville</wicri:cityArea></affiliation><affiliation wicri:level="1"><country wicri:rule="url">États-Unis</country></affiliation><affiliation wicri:level="2"><country xml:lang="fr">États-Unis</country><placeName><region type="state">Floride</region></placeName><wicri:cityArea>Correspondence address: Department of Anatomy and Cell Biology, University of Florida Health Science Center, P.O. Box 100235, Gainesville</wicri:cityArea></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Journal of Cellular Physiology</title><title level="j" type="alt">JOURNAL OF CELLULAR PHYSIOLOGY</title><idno type="ISSN">0021-9541</idno><idno type="eISSN">1097-4652</idno><imprint><biblScope unit="vol">187</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="48">48</biblScope><biblScope unit="page" to="58">58</biblScope><biblScope unit="page-count">11</biblScope><publisher>John Wiley & Sons, Inc.</publisher><pubPlace>New York</pubPlace><date type="published" when="2001-04">2001-04</date></imprint><idno type="ISSN">0021-9541</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0021-9541</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Aldolase B is an abundant cytosolic protein found in all eukaryotic cells. Like many glycolytic enzymes, this protein was sequestered into lysosomes for degradation during nutrient starvation. We report here that the degradation of recombinant aldolase B was enhanced two‐fold when rat and human hepatoma cells were starved for amino acid and serum. In addition, starvation‐induced degradation of aldolase B was inhibited by chloroquine, an inhibitor of lysosomal proteinases and by 3‐methyladenine, an inhibitor of autophagy. Aldolase B has three lysosomal targeting motifs (Q12KKEL, Q58FREL, and IKLDQ111) that have been proposed to interact with hsc73 thereby initiating its transport into lysosomes. In this study, we have mutated the essential glutamine residues in each of these hsc73‐binding motifs in order to evaluate their roles in the lysosomal degradation of aldolase B during starvation. We have found that when glutamines 12 or 58 are mutated to asparagines enhanced degradation of aldolase B proceeded normally. However, when glutamine 111 was mutated to an asparagine or a threonine, starvation‐induced degradation was completely suppressed. These mutations did not appear to alter the tertiary structure of aldolase B since enzymatic activity was not affected. Our results suggest that starvation‐induced lysosomal degradation of aldolase B requires both autophagy and glutamine 111. We discuss the possible roles for autophagy and hsc73‐mediated transport in the lysosomal sequestration of aldolase B. © 2001 Wiley‐Liss, Inc.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>Floride</li></region></list><tree><country name="États-Unis"><region name="Floride"><name sortKey="Susan, P P" sort="Susan, P P" uniqKey="Susan P" first="P. P." last="Susan">P. P. Susan</name></region><name sortKey="Dunn, W A" sort="Dunn, W A" uniqKey="Dunn W" first="W. A." last="Dunn">W. A. Dunn</name><name sortKey="Dunn, W A" sort="Dunn, W A" uniqKey="Dunn W" first="W. A." last="Dunn">W. A. Dunn</name><name sortKey="Dunn, W A" sort="Dunn, W A" uniqKey="Dunn W" first="W. A." last="Dunn">W. A. Dunn</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002316 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 002316 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:19753CA4DB6F5D17B3115B304D72899AA7965C1C
|texte= Starvation‐induced lysosomal degradation of aldolase B requires glutamine 111 in a signal sequence for chaperone‐mediated transport
}}
| This area was generated with Dilib version V0.6.33. |  |