Cationic silica nanoparticles as gene carriers: synthesis, characterization and transfection efficiency in vitro and in vivo.
Identifieur interne : 001F16 ( Main/Exploration ); précédent : 001F15; suivant : 001F17Cationic silica nanoparticles as gene carriers: synthesis, characterization and transfection efficiency in vitro and in vivo.
Auteurs : M N V. Ravi Kumar [Allemagne] ; M. Sameti ; S S Mohapatra ; X. Kong ; R F Lockey ; U. Bakowsky ; G. Lindenblatt ; H. Schmidt ; C M LehrSource :
- Journal of nanoscience and nanotechnology [ 1533-4880 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN (), ADN (métabolisme), Animaux, Cations, Cellules COS, Chloroquine (), Concentration en ions d'hydrogène, Diffusion de rayonnements, Escherichia coli (métabolisme), Lumière, Microscopie à force atomique, Nanostructures (), Nanotechnologie (), Nanotubes (), Photons, Plasmides (métabolisme), Poumon (métabolisme), Protéines à fluorescence verte (métabolisme), Silanes (), Silice (), Silicium (), Souris, Souris de lignée DBA, Spectrophotométrie, Techniques de transfert de gènes, Transfection, Vecteurs génétiques, beta-Galactosidase (métabolisme), Électrophorèse sur gel d'agar.
- MESH :
- métabolisme : ADN, Escherichia coli, Plasmides, Poumon, Protéines à fluorescence verte, beta-Galactosidase.
- ADN, Animaux, Cations, Cellules COS, Chloroquine, Concentration en ions d'hydrogène, Diffusion de rayonnements, Lumière, Microscopie à force atomique, Nanostructures, Nanotechnologie, Nanotubes, Photons, Silanes, Silice, Silicium, Souris, Souris de lignée DBA, Spectrophotométrie, Techniques de transfert de gènes, Transfection, Vecteurs génétiques, Électrophorèse sur gel d'agar.
English descriptors
- KwdEn :
- Animals, COS Cells, Cations, Chloroquine (chemistry), DNA (chemistry), DNA (metabolism), Electrophoresis, Agar Gel, Escherichia coli (metabolism), Gene Transfer Techniques, Genetic Vectors, Green Fluorescent Proteins (metabolism), Hydrogen-Ion Concentration, Light, Lung (metabolism), Mice, Mice, Inbred DBA, Microscopy, Atomic Force, Nanostructures (chemistry), Nanotechnology (methods), Nanotubes (chemistry), Photons, Plasmids (metabolism), Scattering, Radiation, Silanes (chemistry), Silicon (chemistry), Silicon Dioxide (chemistry), Spectrophotometry, Transfection, beta-Galactosidase (metabolism).
- MESH :
- chemical , chemistry : Chloroquine, DNA, Silanes, Silicon, Silicon Dioxide.
- chemical , metabolism : DNA, Green Fluorescent Proteins, beta-Galactosidase.
- chemical : Cations.
- chemistry : Nanostructures, Nanotubes.
- metabolism : Escherichia coli, Lung, Plasmids.
- methods : Nanotechnology.
- Animals, COS Cells, Electrophoresis, Agar Gel, Gene Transfer Techniques, Genetic Vectors, Hydrogen-Ion Concentration, Light, Mice, Mice, Inbred DBA, Microscopy, Atomic Force, Photons, Scattering, Radiation, Spectrophotometry, Transfection.
Abstract
The potential of cationic SiO2 nanoparticles was investigated for in vivo gene transfer in this study. Cationic SiO2 nanoparticles with surface modification were generated using amino-hexyl-amino-propyltri-methoxysilane (AHAPS). The zeta potential of the nanoparticles at pH = 7.4 varied from -31.4 mV (unmodified particles; 10 nm) to +9.6 mV (modified by AHAPS). Complete immobilization of DNA at the nanoparticle surface was achieved at a particle ratio of 80 (w/w nanoparticle/DNA ratio). The surface modified nanoparticle had a size of 42 nm with a distribution from 10-100 nm. The ability of these particles to transfect pCMVbeta reporter gene was tested in Cos-1 cells, and optimum results were obtained in the presence of FCS and chloroquine at a particle ratio of 80. These nanoparticles were tested for their ability to transfer genes in vivo in the mouse lung, and a two-times increase in the expression levels was found with silica particles in comparison to EGFP alone. Very low or no cell toxicity was observed, suggesting silica nanoparticles as potential alternatives for gene transfection.
DOI: 10.1166/jnn.2004.120
PubMed: 15570975
Affiliations:
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Ravi Kumar</name><affiliation wicri:level="3"><nlm:affiliation>Department of Biopharmaceutics and Pharmaceutical Technology, Saarland University Saarbrücken, D 66123, Germany.</nlm:affiliation><country xml:lang="fr">Allemagne</country><wicri:regionArea>Department of Biopharmaceutics and Pharmaceutical Technology, Saarland University Saarbrücken, D 66123</wicri:regionArea><placeName><region type="land" nuts="2">Sarre (Land)</region><settlement type="city">Sarrebruck</settlement></placeName></affiliation></author><author><name sortKey="Sameti, M" sort="Sameti, M" uniqKey="Sameti M" first="M" last="Sameti">M. Sameti</name></author><author><name sortKey="Mohapatra, S S" sort="Mohapatra, S S" uniqKey="Mohapatra S" first="S S" last="Mohapatra">S S Mohapatra</name></author><author><name sortKey="Kong, X" sort="Kong, X" uniqKey="Kong X" first="X" last="Kong">X. Kong</name></author><author><name sortKey="Lockey, R F" sort="Lockey, R F" uniqKey="Lockey R" first="R F" last="Lockey">R F Lockey</name></author><author><name sortKey="Bakowsky, U" sort="Bakowsky, U" uniqKey="Bakowsky U" first="U" last="Bakowsky">U. Bakowsky</name></author><author><name sortKey="Lindenblatt, G" sort="Lindenblatt, G" uniqKey="Lindenblatt G" first="G" last="Lindenblatt">G. Lindenblatt</name></author><author><name sortKey="Schmidt, H" sort="Schmidt, H" uniqKey="Schmidt H" first="H" last="Schmidt">H. Schmidt</name></author><author><name sortKey="Lehr, C M" sort="Lehr, C M" uniqKey="Lehr C" first="C M" last="Lehr">C M Lehr</name></author></analytic><series><title level="j">Journal of nanoscience and nanotechnology</title><idno type="ISSN">1533-4880</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>COS Cells</term><term>Cations</term><term>Chloroquine (chemistry)</term><term>DNA (chemistry)</term><term>DNA (metabolism)</term><term>Electrophoresis, Agar Gel</term><term>Escherichia coli (metabolism)</term><term>Gene Transfer Techniques</term><term>Genetic Vectors</term><term>Green Fluorescent Proteins (metabolism)</term><term>Hydrogen-Ion Concentration</term><term>Light</term><term>Lung (metabolism)</term><term>Mice</term><term>Mice, Inbred DBA</term><term>Microscopy, Atomic Force</term><term>Nanostructures (chemistry)</term><term>Nanotechnology (methods)</term><term>Nanotubes (chemistry)</term><term>Photons</term><term>Plasmids (metabolism)</term><term>Scattering, Radiation</term><term>Silanes (chemistry)</term><term>Silicon (chemistry)</term><term>Silicon Dioxide (chemistry)</term><term>Spectrophotometry</term><term>Transfection</term><term>beta-Galactosidase (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN ()</term><term>ADN (métabolisme)</term><term>Animaux</term><term>Cations</term><term>Cellules COS</term><term>Chloroquine ()</term><term>Concentration en ions d'hydrogène</term><term>Diffusion de rayonnements</term><term>Escherichia coli (métabolisme)</term><term>Lumière</term><term>Microscopie à force atomique</term><term>Nanostructures ()</term><term>Nanotechnologie ()</term><term>Nanotubes ()</term><term>Photons</term><term>Plasmides (métabolisme)</term><term>Poumon (métabolisme)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Silanes ()</term><term>Silice ()</term><term>Silicium ()</term><term>Souris</term><term>Souris de lignée DBA</term><term>Spectrophotométrie</term><term>Techniques de transfert de gènes</term><term>Transfection</term><term>Vecteurs génétiques</term><term>beta-Galactosidase (métabolisme)</term><term>Électrophorèse sur gel d'agar</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Chloroquine</term><term>DNA</term><term>Silanes</term><term>Silicon</term><term>Silicon Dioxide</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>DNA</term><term>Green Fluorescent Proteins</term><term>beta-Galactosidase</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Cations</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nanostructures</term><term>Nanotubes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term><term>Lung</term><term>Plasmids</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Nanotechnology</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN</term><term>Escherichia coli</term><term>Plasmides</term><term>Poumon</term><term>Protéines à fluorescence verte</term><term>beta-Galactosidase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>COS Cells</term><term>Electrophoresis, Agar Gel</term><term>Gene Transfer Techniques</term><term>Genetic Vectors</term><term>Hydrogen-Ion Concentration</term><term>Light</term><term>Mice</term><term>Mice, Inbred DBA</term><term>Microscopy, Atomic Force</term><term>Photons</term><term>Scattering, Radiation</term><term>Spectrophotometry</term><term>Transfection</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN</term><term>Animaux</term><term>Cations</term><term>Cellules COS</term><term>Chloroquine</term><term>Concentration en ions d'hydrogène</term><term>Diffusion de rayonnements</term><term>Lumière</term><term>Microscopie à force atomique</term><term>Nanostructures</term><term>Nanotechnologie</term><term>Nanotubes</term><term>Photons</term><term>Silanes</term><term>Silice</term><term>Silicium</term><term>Souris</term><term>Souris de lignée DBA</term><term>Spectrophotométrie</term><term>Techniques de transfert de gènes</term><term>Transfection</term><term>Vecteurs génétiques</term><term>Électrophorèse sur gel d'agar</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The potential of cationic SiO2 nanoparticles was investigated for in vivo gene transfer in this study. Cationic SiO2 nanoparticles with surface modification were generated using amino-hexyl-amino-propyltri-methoxysilane (AHAPS). The zeta potential of the nanoparticles at pH = 7.4 varied from -31.4 mV (unmodified particles; 10 nm) to +9.6 mV (modified by AHAPS). Complete immobilization of DNA at the nanoparticle surface was achieved at a particle ratio of 80 (w/w nanoparticle/DNA ratio). The surface modified nanoparticle had a size of 42 nm with a distribution from 10-100 nm. The ability of these particles to transfect pCMVbeta reporter gene was tested in Cos-1 cells, and optimum results were obtained in the presence of FCS and chloroquine at a particle ratio of 80. These nanoparticles were tested for their ability to transfer genes in vivo in the mouse lung, and a two-times increase in the expression levels was found with silica particles in comparison to EGFP alone. Very low or no cell toxicity was observed, suggesting silica nanoparticles as potential alternatives for gene transfection.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Sarre (Land)</li></region><settlement><li>Sarrebruck</li></settlement></list><tree><noCountry><name sortKey="Bakowsky, U" sort="Bakowsky, U" uniqKey="Bakowsky U" first="U" last="Bakowsky">U. Bakowsky</name><name sortKey="Kong, X" sort="Kong, X" uniqKey="Kong X" first="X" last="Kong">X. Kong</name><name sortKey="Lehr, C M" sort="Lehr, C M" uniqKey="Lehr C" first="C M" last="Lehr">C M Lehr</name><name sortKey="Lindenblatt, G" sort="Lindenblatt, G" uniqKey="Lindenblatt G" first="G" last="Lindenblatt">G. Lindenblatt</name><name sortKey="Lockey, R F" sort="Lockey, R F" uniqKey="Lockey R" first="R F" last="Lockey">R F Lockey</name><name sortKey="Mohapatra, S S" sort="Mohapatra, S S" uniqKey="Mohapatra S" first="S S" last="Mohapatra">S S Mohapatra</name><name sortKey="Sameti, M" sort="Sameti, M" uniqKey="Sameti M" first="M" last="Sameti">M. Sameti</name><name sortKey="Schmidt, H" sort="Schmidt, H" uniqKey="Schmidt H" first="H" last="Schmidt">H. Schmidt</name></noCountry><country name="Allemagne"><region name="Sarre (Land)"><name sortKey="Ravi Kumar, M N V" sort="Ravi Kumar, M N V" uniqKey="Ravi Kumar M" first="M N V" last="Ravi Kumar">M N V. Ravi Kumar</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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