ChloroquineV1, Main, Exploration, bibRecord, 001C14

Chloroquine inhibits cell growth and induces cell death in A549 lung cancer cells.

Identifieur interne : 001C14 ( Main/Exploration ); précédent : 001C13; suivant : 001C15

Chloroquine inhibits cell growth and induces cell death in A549 lung cancer cells.

Auteurs : Chuandong Fan [République populaire de Chine] ; Weiwei Wang ; Baoxiang Zhao ; Shangli Zhang ; Junying Miao

Source :

Bioorganic & medicinal chemistry [ 0968-0896 ] ; 2006.

RBID : pubmed:16413786

Descripteurs français

KwdFr :
- Chloroquine (pharmacologie), Chloroquine (toxicité), Forme de la cellule, Humains, Lignée cellulaire tumorale, Mort cellulaire (), Prolifération cellulaire (), Tumeurs du poumon (anatomopathologie).
MESH :
- anatomopathologie : Tumeurs du poumon.
- pharmacologie : Chloroquine.
- toxicité : Chloroquine.
- Forme de la cellule, Humains, Lignée cellulaire tumorale, Mort cellulaire, Prolifération cellulaire.

English descriptors

KwdEn :
- Cell Death (drug effects), Cell Line, Tumor, Cell Proliferation (drug effects), Cell Shape, Chloroquine (pharmacology), Chloroquine (toxicity), Humans, Lung Neoplasms (pathology).
MESH :
- chemical , pharmacology : Chloroquine.
- drug effects : Cell Death, Cell Proliferation.
- pathology : Lung Neoplasms.
- chemical , toxicity : Chloroquine.
- Cell Line, Tumor, Cell Shape, Humans.

Abstract

To investigate the effects of chloroquine diphosphate (CQ) on lung cancer cell growth, we treated A549 cells, a lung cancer cell line, with the drug at various concentrations (0.25-128 microM) for 24-72 h. The results showed that, at lower concentrations (from 0.25 to 32 microM), CQ inhibited the growth of A549 cells and, at the same time, it induced vacuolation with increased volume of acidic compartments (VAC). On the other hand, at higher concentrations (64-128 microM), CQ induced apoptosis at 24 h, while its effect of inducing vacuolation declined. The lactate dehydrogenase (LDH) assay showed that with the treatment of CQ 32-64 microM for 72 h or 128 microM for 48 h, CQ induced necrosis of A549 cells. To understand the possible mechanism by which CQ acts in A549 cells, we further incubated the cells with this drug at the concentrations of 32 or 128 microM in the presence of D609, a specific inhibitor of phosphatidylcholine-specific phospholipase C (PC-PLC). The results showed that D609 (50 microM) could inhibit the effects of CQ 32 microM on the viability and VAC, but it could not change the effects of CQ 128 microM on the same. Our data suggested that CQ inhibited A549 lung cancer cell growth at lower concentrations by increasing the volume of lysosomes and that PC-PLC might be involved in this process. The data also indicated that, at higher concentrations, CQ induced apoptosis and necrosis, but at this time its ability to increase the volume of lysosome gradually declined, and PC-PLC might not be implicated in the process.

DOI: 10.1016/j.bmc.2005.12.035
PubMed: 16413786

Affiliations:

République populaire de Chine

Le document en format XML

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<term>Lignée cellulaire tumorale</term>
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<term>Prolifération cellulaire ()</term>
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<front><div type="abstract" xml:lang="en">To investigate the effects of chloroquine diphosphate (CQ) on lung cancer cell growth, we treated A549 cells, a lung cancer cell line, with the drug at various concentrations (0.25-128 microM) for 24-72 h. The results showed that, at lower concentrations (from 0.25 to 32 microM), CQ inhibited the growth of A549 cells and, at the same time, it induced vacuolation with increased volume of acidic compartments (VAC). On the other hand, at higher concentrations (64-128 microM), CQ induced apoptosis at 24 h, while its effect of inducing vacuolation declined. The lactate dehydrogenase (LDH) assay showed that with the treatment of CQ 32-64 microM for 72 h or 128 microM for 48 h, CQ induced necrosis of A549 cells. To understand the possible mechanism by which CQ acts in A549 cells, we further incubated the cells with this drug at the concentrations of 32 or 128 microM in the presence of D609, a specific inhibitor of phosphatidylcholine-specific phospholipase C (PC-PLC). The results showed that D609 (50 microM) could inhibit the effects of CQ 32 microM on the viability and VAC, but it could not change the effects of CQ 128 microM on the same. Our data suggested that CQ inhibited A549 lung cancer cell growth at lower concentrations by increasing the volume of lysosomes and that PC-PLC might be involved in this process. The data also indicated that, at higher concentrations, CQ induced apoptosis and necrosis, but at this time its ability to increase the volume of lysosome gradually declined, and PC-PLC might not be implicated in the process.</div>
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<name sortKey="Zhang, Shangli" sort="Zhang, Shangli" uniqKey="Zhang S" first="Shangli" last="Zhang">Shangli Zhang</name>
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<country name="République populaire de Chine"><noRegion><name sortKey="Fan, Chuandong" sort="Fan, Chuandong" uniqKey="Fan C" first="Chuandong" last="Fan">Chuandong Fan</name>
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Serveur d'exploration Chloroquine

Chloroquine inhibits cell growth and induces cell death in A549 lung cancer cells.

Chloroquine inhibits cell growth and induces cell death in A549 lung cancer cells.

Source :

Descripteurs français

English descriptors

Abstract

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