Non-coding rRNA-mediated preferential killing in cancer cells is enhanced by suppression of autophagy in non-transformed counterpart.
Identifieur interne : 001468 ( Main/Exploration ); précédent : 001467; suivant : 001469Non-coding rRNA-mediated preferential killing in cancer cells is enhanced by suppression of autophagy in non-transformed counterpart.
Auteurs : C J Hwang [États-Unis] ; J R Fields ; Y-H ShiaoSource :
- Cell death & disease [ 2041-4889 ] ; 2011.
Descripteurs français
- KwdFr :
- MESH :
- métabolisme : ARN non traduit, ARN ribosomique, Oligonucléotides.
- pharmacologie : Chloroquine.
- toxicité : Oligonucléotides antisens.
- Animaux, Apoptose, Autophagie, Humains, Lignée cellulaire tumorale, Souris.
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : Oligonucleotides, RNA, Ribosomal, RNA, Untranslated.
- chemical , pharmacology : Chloroquine.
- chemical , toxicity : Oligonucleotides, Antisense.
- Animals, Apoptosis, Autophagy, Cell Line, Tumor, Humans, Mice.
Abstract
Interest to anticancer agents targeting rRNA biogenesis is growing. Cis-non-coding rRNAs, alternative to primary rRNA, have been shown to regulate rRNA biogenesis. We have recently detected bidirectional non-coding rRNAs that carry ribozyme-like properties. Anti-antisense oligonucleotides complementary to antisense non-coding rRNAs markedly stabilized the bidirectional transcripts and induced cell death in mouse lung cells. Here, we demonstrated that the same oligonucleotide killed mouse lung-cancer cells preferentially, compared with non-cancer sister lines, suggesting its potential utility for cancer treatment. A human version of anti-antisense oligonucleotide, complementary to an rDNA intergenic site, mediated apoptosis primarily in cancer cells. Autophagic activation was largely undifferentiable between the anti-antisense and other oligonucleotides and accounted for the undesired cytotoxicity in non-cancer cells. Co-treatment with chloroquine, an autophagy inhibitor, reduced cytotoxicity in the non-cancer cells, but retained the anti-antisense-mediated killings in cancer cells. Furthermore, the anti-antisense oligonucleotide stabilized bidirectional non-coding rRNAs predominantly in human cancer cells and perturbed rRNA biogenesis. Contributions of non-coding rRNAs to cell death were proven by transfection of in -vitro-synthesized transcripts. Taken together, cancer/non-cancer cells respond differently to stabilization of non-coding rRNAs, and such differential responses provide a window of opportunity to enhance anticancer efficacy.
DOI: 10.1038/cddis.2011.110
PubMed: 22158478
Affiliations:
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Le document en format XML
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Non-coding rRNA-mediated preferential killing in cancer cells is enhanced by suppression of autophagy in non-transformed counterpart.</title>
<author><name sortKey="Hwang, C J" sort="Hwang, C J" uniqKey="Hwang C" first="C J" last="Hwang">C J Hwang</name>
<affiliation wicri:level="2"><nlm:affiliation>Laboratory of Comparative Carcinogenesis, National Cancer Institute at Frederick, Frederick, MD, USA.</nlm:affiliation>
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<author><name sortKey="Fields, J R" sort="Fields, J R" uniqKey="Fields J" first="J R" last="Fields">J R Fields</name>
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<author><name sortKey="Shiao, Y H" sort="Shiao, Y H" uniqKey="Shiao Y" first="Y-H" last="Shiao">Y-H Shiao</name>
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<series><title level="j">Cell death & disease</title>
<idno type="eISSN">2041-4889</idno>
<imprint><date when="2011" type="published">2011</date>
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<term>Apoptosis</term>
<term>Autophagy</term>
<term>Cell Line, Tumor</term>
<term>Chloroquine (pharmacology)</term>
<term>Humans</term>
<term>Mice</term>
<term>Oligonucleotides (metabolism)</term>
<term>Oligonucleotides, Antisense (toxicity)</term>
<term>RNA, Ribosomal (metabolism)</term>
<term>RNA, Untranslated (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN non traduit (métabolisme)</term>
<term>ARN ribosomique (métabolisme)</term>
<term>Animaux</term>
<term>Apoptose</term>
<term>Autophagie</term>
<term>Chloroquine (pharmacologie)</term>
<term>Humains</term>
<term>Lignée cellulaire tumorale</term>
<term>Oligonucléotides (métabolisme)</term>
<term>Oligonucléotides antisens (toxicité)</term>
<term>Souris</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Oligonucleotides</term>
<term>RNA, Ribosomal</term>
<term>RNA, Untranslated</term>
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<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Chloroquine</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="toxicity" xml:lang="en"><term>Oligonucleotides, Antisense</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN non traduit</term>
<term>ARN ribosomique</term>
<term>Oligonucléotides</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Chloroquine</term>
</keywords>
<keywords scheme="MESH" qualifier="toxicité" xml:lang="fr"><term>Oligonucléotides antisens</term>
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<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Apoptosis</term>
<term>Autophagy</term>
<term>Cell Line, Tumor</term>
<term>Humans</term>
<term>Mice</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Apoptose</term>
<term>Autophagie</term>
<term>Humains</term>
<term>Lignée cellulaire tumorale</term>
<term>Souris</term>
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<front><div type="abstract" xml:lang="en">Interest to anticancer agents targeting rRNA biogenesis is growing. Cis-non-coding rRNAs, alternative to primary rRNA, have been shown to regulate rRNA biogenesis. We have recently detected bidirectional non-coding rRNAs that carry ribozyme-like properties. Anti-antisense oligonucleotides complementary to antisense non-coding rRNAs markedly stabilized the bidirectional transcripts and induced cell death in mouse lung cells. Here, we demonstrated that the same oligonucleotide killed mouse lung-cancer cells preferentially, compared with non-cancer sister lines, suggesting its potential utility for cancer treatment. A human version of anti-antisense oligonucleotide, complementary to an rDNA intergenic site, mediated apoptosis primarily in cancer cells. Autophagic activation was largely undifferentiable between the anti-antisense and other oligonucleotides and accounted for the undesired cytotoxicity in non-cancer cells. Co-treatment with chloroquine, an autophagy inhibitor, reduced cytotoxicity in the non-cancer cells, but retained the anti-antisense-mediated killings in cancer cells. Furthermore, the anti-antisense oligonucleotide stabilized bidirectional non-coding rRNAs predominantly in human cancer cells and perturbed rRNA biogenesis. Contributions of non-coding rRNAs to cell death were proven by transfection of in -vitro-synthesized transcripts. Taken together, cancer/non-cancer cells respond differently to stabilization of non-coding rRNAs, and such differential responses provide a window of opportunity to enhance anticancer efficacy.</div>
</front>
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<name sortKey="Shiao, Y H" sort="Shiao, Y H" uniqKey="Shiao Y" first="Y-H" last="Shiao">Y-H Shiao</name>
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<country name="États-Unis"><region name="Maryland"><name sortKey="Hwang, C J" sort="Hwang, C J" uniqKey="Hwang C" first="C J" last="Hwang">C J Hwang</name>
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