DMPPQA, a novel angiogenesis inhibitor, induces apoptosis in human colon cancer HCT‐116 cells and HUVECs
Identifieur interne : 001073 ( Main/Exploration ); précédent : 001072; suivant : 001074DMPPQA, a novel angiogenesis inhibitor, induces apoptosis in human colon cancer HCT‐116 cells and HUVECs
Auteurs : Jie Ren ; Hefei Jiang ; Juan Zhao ; Wenqun Xin ; Yuanyuan Xu ; Xin Chen ; Kun HuSource :
- Cell Biology International [ 1065-6995 ] ; 2014-03.
Abstract
Cytotoxic activity of 5,7‐dimethoxy‐2‐phenyl‐N‐propylquinolin‐4‐amine (DMPPQA) was investigated in human colon cancer cells HCT‐116 and umbilical vein endothelial cell line HUVEC. The IC50 of DMPPQA on HCT‐116 and HUVEC cells were respectively 1.26 and 7.43 µM after 72 h treatment. DMPPQA inhibited the growth of HCT‐116 and HUVEC cells in concentration‐ and time‐dependent manners. Typical morphological changes of apoptotic body formation were seen after DMPPQA with Hoechst 33258 staining. FCM analysis showed that DMPPQA induced apoptosis, mitochondrial membrane potential loss (ΔΨm) and increase in the production of intracellular reactive oxygen species (ROS) of HCT‐116 cells. After treating with DMPPQA, apoptosis‐related protein expression of Bax, cytochrome c, caspase‐9, caspase‐3, PARP‐1 and P53 increased and Bcl‐2 protein expression decreased. DMPPQA treatment of HUVECs reduced cell migration and microcapillary tube formation in a Matrigel matrix. It also decreased VEGF protein expression. Thus DMPPQA acts as an angiogenesis inhibitor and induces cell apoptosis by a caspase‐dependent mitochondrial pathway.
Url:
DOI: 10.1002/cbin.10209
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000394
- to stream Istex, to step Curation: 000394
- to stream Istex, to step Checkpoint: 000166
- to stream Main, to step Merge: 001074
- to stream Main, to step Curation: 001073
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">DMPPQA, a novel angiogenesis inhibitor, induces apoptosis in human colon cancer HCT‐116 cells and HUVECs</title>
<author><name sortKey="Ren, Jie" sort="Ren, Jie" uniqKey="Ren J" first="Jie" last="Ren">Jie Ren</name>
</author>
<author><name sortKey="Jiang, Hefei" sort="Jiang, Hefei" uniqKey="Jiang H" first="Hefei" last="Jiang">Hefei Jiang</name>
</author>
<author><name sortKey="Zhao, Juan" sort="Zhao, Juan" uniqKey="Zhao J" first="Juan" last="Zhao">Juan Zhao</name>
</author>
<author><name sortKey="Xin, Wenqun" sort="Xin, Wenqun" uniqKey="Xin W" first="Wenqun" last="Xin">Wenqun Xin</name>
</author>
<author><name sortKey="Xu, Yuanyuan" sort="Xu, Yuanyuan" uniqKey="Xu Y" first="Yuanyuan" last="Xu">Yuanyuan Xu</name>
</author>
<author><name sortKey="Chen, Xin" sort="Chen, Xin" uniqKey="Chen X" first="Xin" last="Chen">Xin Chen</name>
</author>
<author><name sortKey="Hu, Kun" sort="Hu, Kun" uniqKey="Hu K" first="Kun" last="Hu">Kun Hu</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:8E8899FCB0D7AFCF310D3D1AC8CD4C12302F5117</idno>
<date when="2014" year="2014">2014</date>
<idno type="doi">10.1002/cbin.10209</idno>
<idno type="url">https://api.istex.fr/ark:/67375/WNG-TSV254Z3-H/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">000394</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000394</idno>
<idno type="wicri:Area/Istex/Curation">000394</idno>
<idno type="wicri:Area/Istex/Checkpoint">000166</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000166</idno>
<idno type="wicri:doubleKey">1065-6995:2014:Ren J:dmppqa:a:novel</idno>
<idno type="wicri:Area/Main/Merge">001074</idno>
<idno type="wicri:Area/Main/Curation">001073</idno>
<idno type="wicri:Area/Main/Exploration">001073</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">DMPPQA, a novel angiogenesis inhibitor, induces apoptosis in human colon cancer HCT‐116 cells and HUVECs</title>
<author><name sortKey="Ren, Jie" sort="Ren, Jie" uniqKey="Ren J" first="Jie" last="Ren">Jie Ren</name>
<affiliation></affiliation>
<affiliation><wicri:noCountry code="no comma">E-mail: renjie2006@163.com</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Jiang, Hefei" sort="Jiang, Hefei" uniqKey="Jiang H" first="Hefei" last="Jiang">Hefei Jiang</name>
<affiliation><wicri:noCountry code="subField">Jiangsu</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Zhao, Juan" sort="Zhao, Juan" uniqKey="Zhao J" first="Juan" last="Zhao">Juan Zhao</name>
<affiliation><wicri:noCountry code="subField">Jiangsu</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Xin, Wenqun" sort="Xin, Wenqun" uniqKey="Xin W" first="Wenqun" last="Xin">Wenqun Xin</name>
<affiliation><wicri:noCountry code="subField">Jiangsu</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Xu, Yuanyuan" sort="Xu, Yuanyuan" uniqKey="Xu Y" first="Yuanyuan" last="Xu">Yuanyuan Xu</name>
<affiliation><wicri:noCountry code="subField">Jiangsu</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Chen, Xin" sort="Chen, Xin" uniqKey="Chen X" first="Xin" last="Chen">Xin Chen</name>
<affiliation><wicri:noCountry code="subField">Jiangsu</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Hu, Kun" sort="Hu, Kun" uniqKey="Hu K" first="Kun" last="Hu">Kun Hu</name>
<affiliation></affiliation>
<affiliation><wicri:noCountry code="no comma">E-mail: renjie2006@163.com</wicri:noCountry>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Cell Biology International</title>
<title level="j" type="alt">CELL BIOLOGY INTERNATIONAL</title>
<idno type="ISSN">1065-6995</idno>
<idno type="eISSN">1095-8355</idno>
<imprint><biblScope unit="vol">38</biblScope>
<biblScope unit="issue">3</biblScope>
<biblScope unit="page" from="343">343</biblScope>
<biblScope unit="page" to="354">354</biblScope>
<biblScope unit="page-count">12</biblScope>
<date type="published" when="2014-03">2014-03</date>
</imprint>
<idno type="ISSN">1065-6995</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">1065-6995</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Cytotoxic activity of 5,7‐dimethoxy‐2‐phenyl‐N‐propylquinolin‐4‐amine (DMPPQA) was investigated in human colon cancer cells HCT‐116 and umbilical vein endothelial cell line HUVEC. The IC50 of DMPPQA on HCT‐116 and HUVEC cells were respectively 1.26 and 7.43 µM after 72 h treatment. DMPPQA inhibited the growth of HCT‐116 and HUVEC cells in concentration‐ and time‐dependent manners. Typical morphological changes of apoptotic body formation were seen after DMPPQA with Hoechst 33258 staining. FCM analysis showed that DMPPQA induced apoptosis, mitochondrial membrane potential loss (ΔΨm) and increase in the production of intracellular reactive oxygen species (ROS) of HCT‐116 cells. After treating with DMPPQA, apoptosis‐related protein expression of Bax, cytochrome c, caspase‐9, caspase‐3, PARP‐1 and P53 increased and Bcl‐2 protein expression decreased. DMPPQA treatment of HUVECs reduced cell migration and microcapillary tube formation in a Matrigel matrix. It also decreased VEGF protein expression. Thus DMPPQA acts as an angiogenesis inhibitor and induces cell apoptosis by a caspase‐dependent mitochondrial pathway.</div>
</front>
</TEI>
<affiliations><list></list>
<tree><noCountry><name sortKey="Chen, Xin" sort="Chen, Xin" uniqKey="Chen X" first="Xin" last="Chen">Xin Chen</name>
<name sortKey="Hu, Kun" sort="Hu, Kun" uniqKey="Hu K" first="Kun" last="Hu">Kun Hu</name>
<name sortKey="Jiang, Hefei" sort="Jiang, Hefei" uniqKey="Jiang H" first="Hefei" last="Jiang">Hefei Jiang</name>
<name sortKey="Ren, Jie" sort="Ren, Jie" uniqKey="Ren J" first="Jie" last="Ren">Jie Ren</name>
<name sortKey="Xin, Wenqun" sort="Xin, Wenqun" uniqKey="Xin W" first="Wenqun" last="Xin">Wenqun Xin</name>
<name sortKey="Xu, Yuanyuan" sort="Xu, Yuanyuan" uniqKey="Xu Y" first="Yuanyuan" last="Xu">Yuanyuan Xu</name>
<name sortKey="Zhao, Juan" sort="Zhao, Juan" uniqKey="Zhao J" first="Juan" last="Zhao">Juan Zhao</name>
</noCountry>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001073 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001073 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= ChloroquineV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:8E8899FCB0D7AFCF310D3D1AC8CD4C12302F5117 |texte= DMPPQA, a novel angiogenesis inhibitor, induces apoptosis in human colon cancer HCT‐116 cells and HUVECs }}
This area was generated with Dilib version V0.6.33. |