Airway contractility in the precision-cut lung slice after cryopreservation.
Identifieur interne : 001038 ( Main/Exploration ); précédent : 001037; suivant : 001039Airway contractility in the precision-cut lung slice after cryopreservation.
Auteurs : Sonia R. Rosner [États-Unis] ; Sumati Ram-Mohan ; Jesus R. Paez-Cortez ; Tera L. Lavoie ; Maria L. Dowell ; Lei Yuan ; Xingbin Ai ; Alan Fine ; William C. Aird ; Julian Solway ; Jeffrey J. Fredberg ; Ramaswamy KrishnanSource :
- American journal of respiratory cell and molecular biology [ 1535-4989 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- physiologie : Contraction musculaire, Mort cellulaire, Poumon, Survie cellulaire.
- Animaux, Congélation, Cryoconservation, Souris, Souris de lignée C57BL.
English descriptors
- KwdEn :
- MESH :
- methods : Cryopreservation.
- physiology : Cell Death, Cell Survival, Lung, Muscle Contraction.
- Animals, Freezing, Mice, Mice, Inbred C57BL.
Abstract
An emerging tool in airway biology is the precision-cut lung slice (PCLS). Adoption of the PCLS as a model for assessing airway reactivity has been hampered by the limited time window within which tissues remain viable. Here we demonstrate that the PCLS can be frozen, stored long-term, and then thawed for later experimental use. Compared with the never-frozen murine PCLS, the frozen-thawed PCLS shows metabolic activity that is decreased to an extent comparable to that observed in other cryopreserved tissues but shows no differences in cell viability or in airway caliber responses to the contractile agonist methacholine or the relaxing agonist chloroquine. These results indicate that freezing and long-term storage is a feasible solution to the problem of limited viability of the PCLS in culture.
DOI: 10.1165/rcmb.2013-0166MA
PubMed: 24313705
Affiliations:
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Le document en format XML
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