Respiratory syncytial virus induces indoleamine 2,3‐dioxygenase activity: a potential novel role in the development of allergic disease
Identifieur interne : 000F28 ( Main/Exploration ); précédent : 000F27; suivant : 000F29Respiratory syncytial virus induces indoleamine 2,3‐dioxygenase activity: a potential novel role in the development of allergic disease
Auteurs : F. Ajamian [Canada] ; Y. Wu [Canada] ; C. Ebeling [Canada] ; R. Ilarraza [Canada] ; S. O. Odemuyiwa [Canada] ; R. Moqbel [Canada] ; D. J. Adamko [Canada]Source :
- Clinical & Experimental Allergy [ 0954-7894 ] ; 2015-03.
Abstract
Background: Infants that develop severe bronchiolitis due to respiratory syncytial virus (RSV) are at increased risk of developing asthma later in life. We investigated a potential immunological mechanism for the association between RSV and the development of allergic inflammation. The enzyme indoleamine 2,3‐dioxygenase (IDO) has been reported to induce selective apoptosis of T helper 1 (Th1) cells and contributed to Th2‐biased immune responses. Objective: To determine whether RSV infection in vitro could induce IDO expression and bioactivity in human dendritic cells, leading to a Th2‐biased immune response. Methods: Human peripheral blood monocytes from healthy adult donors were isolated, differentiated to dendritic cells (moDC), in vitro. We studied RSV infection and mechanisms of IDO activation in moDC with subsequent effect on T‐bet expression. Results: We found that moDC were infected by RSV and that this induced IDO activation. RSV‐induced IDO activity was inhibited by palivizumab, UV inactivation, TL4R inhibition, and ribavirin. However, blocking endosomal TLR function with chloroquine did not inhibit IDO activity. Selective inhibitors suggested that RSV‐induced IDO activity was dependent on the retinoic acid‐inducible gene‐I (RIG‐I) related pathway via NF‐κB and p38 MAPK. Coculture of RSV‐infected moDC with activated T cells, in a transwell system, suppressed expression of T‐bet (a Th1‐associated factor) but not GATA3 (a Th2 regulator). Inhibition of IDO activity with the competitive inhibitor, 1‐methyl tryptophan, blocked the effect on T‐bet expression. Conclusion and Clinical Relevance: Our data show for the first time that RSV can induce the expression and bioactivity of IDO in human moDC, in a virus replication‐dependant fashion. We suggest that RSV activation of IDO could be a potential mechanism for the development of allergic diseases.
Url:
DOI: 10.1111/cea.12498
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 001236
- to stream Istex, to step Curation: 001236
- to stream Istex, to step Checkpoint: 000073
- to stream Main, to step Merge: 000F29
- to stream Main, to step Curation: 000F28
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Respiratory syncytial virus induces indoleamine 2,3‐dioxygenase activity: a potential novel role in the development of allergic disease</title>
<author><name sortKey="Ajamian, F" sort="Ajamian, F" uniqKey="Ajamian F" first="F." last="Ajamian">F. Ajamian</name>
</author>
<author><name sortKey="Wu, Y" sort="Wu, Y" uniqKey="Wu Y" first="Y." last="Wu">Y. Wu</name>
</author>
<author><name sortKey="Ebeling, C" sort="Ebeling, C" uniqKey="Ebeling C" first="C." last="Ebeling">C. Ebeling</name>
</author>
<author><name sortKey="Ilarraza, R" sort="Ilarraza, R" uniqKey="Ilarraza R" first="R." last="Ilarraza">R. Ilarraza</name>
</author>
<author><name sortKey="Odemuyiwa, S O" sort="Odemuyiwa, S O" uniqKey="Odemuyiwa S" first="S. O." last="Odemuyiwa">S. O. Odemuyiwa</name>
</author>
<author><name sortKey="Moqbel, R" sort="Moqbel, R" uniqKey="Moqbel R" first="R." last="Moqbel">R. Moqbel</name>
</author>
<author><name sortKey="Adamko, D J" sort="Adamko, D J" uniqKey="Adamko D" first="D. J." last="Adamko">D. J. Adamko</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">ISTEX</idno>
<idno type="RBID">ISTEX:560EE21088075BEAE1C3CC1BC410F2BBA9DA5C25</idno>
<date when="2015" year="2015">2015</date>
<idno type="doi">10.1111/cea.12498</idno>
<idno type="url">https://api.istex.fr/ark:/67375/WNG-SC54FJJX-V/fulltext.pdf</idno>
<idno type="wicri:Area/Istex/Corpus">001236</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001236</idno>
<idno type="wicri:Area/Istex/Curation">001236</idno>
<idno type="wicri:Area/Istex/Checkpoint">000073</idno>
<idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000073</idno>
<idno type="wicri:doubleKey">0954-7894:2015:Ajamian F:respiratory:syncytial:virus</idno>
<idno type="wicri:Area/Main/Merge">000F29</idno>
<idno type="wicri:Area/Main/Curation">000F28</idno>
<idno type="wicri:Area/Main/Exploration">000F28</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title level="a" type="main">Respiratory syncytial virus induces indoleamine 2,3‐dioxygenase activity: a potential novel role in the development of allergic disease</title>
<author><name sortKey="Ajamian, F" sort="Ajamian, F" uniqKey="Ajamian F" first="F." last="Ajamian">F. Ajamian</name>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Pulmonary Research Group, University of Alberta, Edmonton, Alberta</wicri:regionArea>
<wicri:noRegion>Alberta</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Medicine, University of Alberta, Alberta, Edmonton</wicri:regionArea>
<wicri:noRegion>Edmonton</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wu, Y" sort="Wu, Y" uniqKey="Wu Y" first="Y." last="Wu">Y. Wu</name>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Pulmonary Research Group, University of Alberta, Edmonton, Alberta</wicri:regionArea>
<wicri:noRegion>Alberta</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Pediatrics, University of Alberta, Alberta, Edmonton</wicri:regionArea>
<wicri:noRegion>Edmonton</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ebeling, C" sort="Ebeling, C" uniqKey="Ebeling C" first="C." last="Ebeling">C. Ebeling</name>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Pulmonary Research Group, University of Alberta, Edmonton, Alberta</wicri:regionArea>
<wicri:noRegion>Alberta</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Pediatrics, University of Alberta, Alberta, Edmonton</wicri:regionArea>
<wicri:noRegion>Edmonton</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ilarraza, R" sort="Ilarraza, R" uniqKey="Ilarraza R" first="R." last="Ilarraza">R. Ilarraza</name>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Pulmonary Research Group, University of Alberta, Edmonton, Alberta</wicri:regionArea>
<wicri:noRegion>Alberta</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Pediatrics, University of Alberta, Alberta, Edmonton</wicri:regionArea>
<wicri:noRegion>Edmonton</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Odemuyiwa, S O" sort="Odemuyiwa, S O" uniqKey="Odemuyiwa S" first="S. O." last="Odemuyiwa">S. O. Odemuyiwa</name>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Pulmonary Research Group, University of Alberta, Edmonton, Alberta</wicri:regionArea>
<wicri:noRegion>Alberta</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Medicine, University of Alberta, Alberta, Edmonton</wicri:regionArea>
<wicri:noRegion>Edmonton</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Moqbel, R" sort="Moqbel, R" uniqKey="Moqbel R" first="R." last="Moqbel">R. Moqbel</name>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Pulmonary Research Group, University of Alberta, Edmonton, Alberta</wicri:regionArea>
<wicri:noRegion>Alberta</wicri:noRegion>
</affiliation>
<affiliation wicri:level="4"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Immunology, University of Manitoba, Manitoba, Winnipeg</wicri:regionArea>
<orgName type="university">Université du Manitoba</orgName>
<placeName><settlement type="city">Winnipeg</settlement>
<region type="state">Manitoba</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Adamko, D J" sort="Adamko, D J" uniqKey="Adamko D" first="D. J." last="Adamko">D. J. Adamko</name>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Pulmonary Research Group, University of Alberta, Edmonton, Alberta</wicri:regionArea>
<wicri:noRegion>Alberta</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Medicine, University of Alberta, Edmonton, Alberta</wicri:regionArea>
<wicri:noRegion>Alberta</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Pediatrics, University of Alberta, Edmonton, Alberta</wicri:regionArea>
<wicri:noRegion>Alberta</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><country xml:lang="fr">Canada</country>
<wicri:regionArea>Department of Pediatrics, University of Saskatchewan, Saskatchewan, Saskatoon</wicri:regionArea>
<wicri:noRegion>Saskatoon</wicri:noRegion>
</affiliation>
<affiliation></affiliation>
<affiliation wicri:level="1"><country wicri:rule="url">Canada</country>
</affiliation>
</author>
</analytic>
<monogr></monogr>
<series><title level="j" type="main">Clinical & Experimental Allergy</title>
<title level="j" type="alt">CLINICAL AND EXPERIMENTAL ALLERGY</title>
<idno type="ISSN">0954-7894</idno>
<idno type="eISSN">1365-2222</idno>
<imprint><biblScope unit="vol">45</biblScope>
<biblScope unit="issue">3</biblScope>
<biblScope unit="page" from="644">644</biblScope>
<biblScope unit="page" to="659">659</biblScope>
<biblScope unit="page-count">16</biblScope>
<date type="published" when="2015-03">2015-03</date>
</imprint>
<idno type="ISSN">0954-7894</idno>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><idno type="ISSN">0954-7894</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract">Background: Infants that develop severe bronchiolitis due to respiratory syncytial virus (RSV) are at increased risk of developing asthma later in life. We investigated a potential immunological mechanism for the association between RSV and the development of allergic inflammation. The enzyme indoleamine 2,3‐dioxygenase (IDO) has been reported to induce selective apoptosis of T helper 1 (Th1) cells and contributed to Th2‐biased immune responses. Objective: To determine whether RSV infection in vitro could induce IDO expression and bioactivity in human dendritic cells, leading to a Th2‐biased immune response. Methods: Human peripheral blood monocytes from healthy adult donors were isolated, differentiated to dendritic cells (moDC), in vitro. We studied RSV infection and mechanisms of IDO activation in moDC with subsequent effect on T‐bet expression. Results: We found that moDC were infected by RSV and that this induced IDO activation. RSV‐induced IDO activity was inhibited by palivizumab, UV inactivation, TL4R inhibition, and ribavirin. However, blocking endosomal TLR function with chloroquine did not inhibit IDO activity. Selective inhibitors suggested that RSV‐induced IDO activity was dependent on the retinoic acid‐inducible gene‐I (RIG‐I) related pathway via NF‐κB and p38 MAPK. Coculture of RSV‐infected moDC with activated T cells, in a transwell system, suppressed expression of T‐bet (a Th1‐associated factor) but not GATA3 (a Th2 regulator). Inhibition of IDO activity with the competitive inhibitor, 1‐methyl tryptophan, blocked the effect on T‐bet expression. Conclusion and Clinical Relevance: Our data show for the first time that RSV can induce the expression and bioactivity of IDO in human moDC, in a virus replication‐dependant fashion. We suggest that RSV activation of IDO could be a potential mechanism for the development of allergic diseases.</div>
</front>
</TEI>
<affiliations><list><country><li>Canada</li>
</country>
<region><li>Manitoba</li>
</region>
<settlement><li>Winnipeg</li>
</settlement>
<orgName><li>Université du Manitoba</li>
</orgName>
</list>
<tree><country name="Canada"><noRegion><name sortKey="Ajamian, F" sort="Ajamian, F" uniqKey="Ajamian F" first="F." last="Ajamian">F. Ajamian</name>
</noRegion>
<name sortKey="Adamko, D J" sort="Adamko, D J" uniqKey="Adamko D" first="D. J." last="Adamko">D. J. Adamko</name>
<name sortKey="Adamko, D J" sort="Adamko, D J" uniqKey="Adamko D" first="D. J." last="Adamko">D. J. Adamko</name>
<name sortKey="Adamko, D J" sort="Adamko, D J" uniqKey="Adamko D" first="D. J." last="Adamko">D. J. Adamko</name>
<name sortKey="Adamko, D J" sort="Adamko, D J" uniqKey="Adamko D" first="D. J." last="Adamko">D. J. Adamko</name>
<name sortKey="Adamko, D J" sort="Adamko, D J" uniqKey="Adamko D" first="D. J." last="Adamko">D. J. Adamko</name>
<name sortKey="Ajamian, F" sort="Ajamian, F" uniqKey="Ajamian F" first="F." last="Ajamian">F. Ajamian</name>
<name sortKey="Ebeling, C" sort="Ebeling, C" uniqKey="Ebeling C" first="C." last="Ebeling">C. Ebeling</name>
<name sortKey="Ebeling, C" sort="Ebeling, C" uniqKey="Ebeling C" first="C." last="Ebeling">C. Ebeling</name>
<name sortKey="Ilarraza, R" sort="Ilarraza, R" uniqKey="Ilarraza R" first="R." last="Ilarraza">R. Ilarraza</name>
<name sortKey="Ilarraza, R" sort="Ilarraza, R" uniqKey="Ilarraza R" first="R." last="Ilarraza">R. Ilarraza</name>
<name sortKey="Moqbel, R" sort="Moqbel, R" uniqKey="Moqbel R" first="R." last="Moqbel">R. Moqbel</name>
<name sortKey="Moqbel, R" sort="Moqbel, R" uniqKey="Moqbel R" first="R." last="Moqbel">R. Moqbel</name>
<name sortKey="Odemuyiwa, S O" sort="Odemuyiwa, S O" uniqKey="Odemuyiwa S" first="S. O." last="Odemuyiwa">S. O. Odemuyiwa</name>
<name sortKey="Odemuyiwa, S O" sort="Odemuyiwa, S O" uniqKey="Odemuyiwa S" first="S. O." last="Odemuyiwa">S. O. Odemuyiwa</name>
<name sortKey="Wu, Y" sort="Wu, Y" uniqKey="Wu Y" first="Y." last="Wu">Y. Wu</name>
<name sortKey="Wu, Y" sort="Wu, Y" uniqKey="Wu Y" first="Y." last="Wu">Y. Wu</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000F28 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000F28 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= ChloroquineV1 |flux= Main |étape= Exploration |type= RBID |clé= ISTEX:560EE21088075BEAE1C3CC1BC410F2BBA9DA5C25 |texte= Respiratory syncytial virus induces indoleamine 2,3‐dioxygenase activity: a potential novel role in the development of allergic disease }}
This area was generated with Dilib version V0.6.33. |