Ubiquitin-coated nanodiamonds bind to autophagy receptors for entry into the selective autophagy pathway.
Identifieur interne : 000C88 ( Main/Exploration ); précédent : 000C87; suivant : 000C89Ubiquitin-coated nanodiamonds bind to autophagy receptors for entry into the selective autophagy pathway.
Auteurs : Kuang-Kai Liu [Taïwan] ; Wei-Ru Qiu [Taïwan] ; Emmanuel Naveen Raj [Taïwan] ; Huei-Fang Liu [Taïwan] ; Hou-Syun Huang [Taïwan] ; Yu-Wei Lin [Taïwan] ; Chien-Jen Chang [Taïwan] ; Ting-Hua Chen [Taïwan] ; Chinpiao Chen [Taïwan] ; Huan-Cheng Chang [Taïwan] ; Jenn-Kang Hwang [Taïwan] ; Jui-I Chao [Taïwan]Source :
- Autophagy [ 1554-8635 ] ; 2017.
Descripteurs français
- KwdFr :
- Animaux, Autophagie, Cellules A549, Humains, Liaison aux protéines, Lignée cellulaire tumorale, Lysosomes (métabolisme), Microscopie confocale, Mort cellulaire, Nanodiamants (), Protéines associées aux microtubules (), Protéines de tissu nerveux (), Protéines nucléaires (), Protéines recombinantes (), Protéines à fluorescence verte (), Récepteurs cytoplasmiques et nucléaires (), Souris, Souris nude, Séquestosome-1 (), Transplantation tumorale, Tumeurs du poumon (métabolisme), Ubiquitine ().
- MESH :
- métabolisme : Lysosomes, Tumeurs du poumon.
- Animaux, Autophagie, Cellules A549, Humains, Liaison aux protéines, Lignée cellulaire tumorale, Microscopie confocale, Mort cellulaire, Nanodiamants, Protéines associées aux microtubules, Protéines de tissu nerveux, Protéines nucléaires, Protéines recombinantes, Protéines à fluorescence verte, Récepteurs cytoplasmiques et nucléaires, Souris, Souris nude, Séquestosome-1, Transplantation tumorale, Ubiquitine.
English descriptors
- KwdEn :
- A549 Cells, Animals, Autophagy, Cell Death, Cell Line, Tumor, Cellular Senescence, Green Fluorescent Proteins (chemistry), Humans, Lung Neoplasms (metabolism), Lysosomes (metabolism), Mice, Mice, Nude, Microscopy, Confocal, Microtubule-Associated Proteins (chemistry), Nanodiamonds (chemistry), Neoplasm Transplantation, Nerve Tissue Proteins (chemistry), Nuclear Proteins (chemistry), Protein Binding, Receptors, Cytoplasmic and Nuclear (chemistry), Recombinant Proteins (chemistry), Sequestosome-1 Protein (chemistry), Ubiquitin (chemistry).
- MESH :
- chemical , chemistry : Green Fluorescent Proteins, Microtubule-Associated Proteins, Nanodiamonds, Nerve Tissue Proteins, Nuclear Proteins, Receptors, Cytoplasmic and Nuclear, Recombinant Proteins, Sequestosome-1 Protein, Ubiquitin.
- metabolism : Lung Neoplasms, Lysosomes.
- A549 Cells, Animals, Autophagy, Cell Death, Cell Line, Tumor, Cellular Senescence, Humans, Mice, Mice, Nude, Microscopy, Confocal, Neoplasm Transplantation, Protein Binding.
Abstract
Selective macroautophagy/autophagy plays a pivotal role in the processing of foreign pathogens and cellular components to maintain homeostasis in human cells. To date, numerous studies have demonstrated the uptake of nanoparticles by cells, but their intracellular processing through selective autophagy remains unclear. Here we show that carbon-based nanodiamonds (NDs) coated with ubiquitin (Ub) bind to autophagy receptors (SQSTM1 [sequestosome 1], OPTN [optineurin], and CALCOCO2/NDP52 [calcium binding and coiled-coil domain 2]) and are then linked to MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3) for entry into the selective autophagy pathway. NDs are ultimately delivered to lysosomes. Ectopically expressed SQSTM1-green fluorescence protein (GFP) could bind to the Ub-coated NDs. By contrast, the Ub-associated domain mutant of SQSTM1 (ΔUBA)-GFP did not bind to the Ub-coated NDs. Chloroquine, an autophagy inhibitor, prevented the ND-containing autophagosomes from fusing with lysosomes. Furthermore, autophagy receptors OPTN and CALCOCO2/NDP52, involved in the processing of bacteria, were found to be involved in the selective autophagy of NDs. However, ND particles located in the lysosomes of cells did not induce mitotic blockage, senescence, or cell death. Single ND clusters in the lysosomes of cells were observed in the xenografted human lung tumors of nude mice. This study demonstrated for the first time that Ub-coated nanoparticles bind to autophagy receptors for entry into the selective autophagy pathway, facilitating their delivery to lysosomes.
DOI: 10.1080/15548627.2016.1254864
PubMed: 27846374
Affiliations:
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</wicri:noRegion></affiliation></author><author><name sortKey="Huang, Hou Syun" sort="Huang, Hou Syun" uniqKey="Huang H" first="Hou-Syun" last="Huang">Hou-Syun Huang</name><affiliation wicri:level="1"><nlm:affiliation>a Department and Institute of Biological Science and Technology , National Chiao Tung University , Hsinchu , Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>a Department and Institute of Biological Science and Technology , National Chiao Tung University , Hsinchu </wicri:regionArea><wicri:noRegion>Hsinchu </wicri:noRegion></affiliation></author><author><name sortKey="Lin, Yu Wei" sort="Lin, Yu Wei" uniqKey="Lin Y" first="Yu-Wei" last="Lin">Yu-Wei Lin</name><affiliation wicri:level="1"><nlm:affiliation>b Institute of Molecular Medicine and Bioengineering, National Chiao Tung University , Hsinchu , Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>b Institute of Molecular Medicine and Bioengineering, National Chiao Tung University , Hsinchu </wicri:regionArea><wicri:noRegion>Hsinchu </wicri:noRegion></affiliation></author><author><name sortKey="Chang, Chien Jen" sort="Chang, Chien Jen" uniqKey="Chang C" first="Chien-Jen" last="Chang">Chien-Jen Chang</name><affiliation wicri:level="1"><nlm:affiliation>a Department and Institute of Biological Science and Technology , National Chiao Tung University , Hsinchu , Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>a Department and Institute of Biological Science and Technology , National Chiao Tung University , Hsinchu </wicri:regionArea><wicri:noRegion>Hsinchu </wicri:noRegion></affiliation></author><author><name sortKey="Chen, Ting Hua" sort="Chen, Ting Hua" uniqKey="Chen T" first="Ting-Hua" last="Chen">Ting-Hua Chen</name><affiliation wicri:level="1"><nlm:affiliation>a Department and Institute of Biological Science and Technology , National Chiao Tung University , Hsinchu , Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>a Department and Institute of Biological Science and Technology , National Chiao Tung University , Hsinchu </wicri:regionArea><wicri:noRegion>Hsinchu </wicri:noRegion></affiliation></author><author><name sortKey="Chen, Chinpiao" sort="Chen, Chinpiao" uniqKey="Chen C" first="Chinpiao" last="Chen">Chinpiao Chen</name><affiliation wicri:level="1"><nlm:affiliation>c Department of Chemistry , National Dong Hwa University , Hualien , Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>c Department of Chemistry , National Dong Hwa University , Hualien </wicri:regionArea><wicri:noRegion>Hualien </wicri:noRegion></affiliation></author><author><name sortKey="Chang, Huan Cheng" sort="Chang, Huan Cheng" uniqKey="Chang H" first="Huan-Cheng" last="Chang">Huan-Cheng Chang</name><affiliation wicri:level="1"><nlm:affiliation>d Institute of Atomic and Molecular Sciences, Academia Sinica , Taipei , Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>d Institute of Atomic and Molecular Sciences, Academia Sinica , Taipei </wicri:regionArea><wicri:noRegion>Taipei </wicri:noRegion></affiliation></author><author><name sortKey="Hwang, Jenn Kang" sort="Hwang, Jenn Kang" uniqKey="Hwang J" first="Jenn-Kang" last="Hwang">Jenn-Kang Hwang</name><affiliation wicri:level="1"><nlm:affiliation>e Institute of Bioinformatics and Systems Biology, National Chiao Tung University , Hsinchu , Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>e Institute of Bioinformatics and Systems Biology, National Chiao Tung University , Hsinchu </wicri:regionArea><wicri:noRegion>Hsinchu </wicri:noRegion></affiliation></author><author><name sortKey="Chao, Jui I" sort="Chao, Jui I" uniqKey="Chao J" first="Jui-I" last="Chao">Jui-I Chao</name><affiliation wicri:level="1"><nlm:affiliation>a Department and Institute of Biological Science and Technology , National Chiao Tung University , Hsinchu , Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>a Department and Institute of Biological Science and Technology , National Chiao Tung University , Hsinchu </wicri:regionArea><wicri:noRegion>Hsinchu </wicri:noRegion></affiliation></author></analytic><series><title level="j">Autophagy</title><idno type="eISSN">1554-8635</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>A549 Cells</term><term>Animals</term><term>Autophagy</term><term>Cell Death</term><term>Cell Line, Tumor</term><term>Cellular Senescence</term><term>Green Fluorescent Proteins (chemistry)</term><term>Humans</term><term>Lung Neoplasms (metabolism)</term><term>Lysosomes (metabolism)</term><term>Mice</term><term>Mice, Nude</term><term>Microscopy, Confocal</term><term>Microtubule-Associated Proteins (chemistry)</term><term>Nanodiamonds (chemistry)</term><term>Neoplasm Transplantation</term><term>Nerve Tissue Proteins (chemistry)</term><term>Nuclear Proteins (chemistry)</term><term>Protein Binding</term><term>Receptors, Cytoplasmic and Nuclear (chemistry)</term><term>Recombinant Proteins (chemistry)</term><term>Sequestosome-1 Protein (chemistry)</term><term>Ubiquitin (chemistry)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Autophagie</term><term>Cellules A549</term><term>Humains</term><term>Liaison aux protéines</term><term>Lignée cellulaire tumorale</term><term>Lysosomes (métabolisme)</term><term>Microscopie confocale</term><term>Mort cellulaire</term><term>Nanodiamants ()</term><term>Protéines associées aux microtubules ()</term><term>Protéines de tissu nerveux ()</term><term>Protéines nucléaires ()</term><term>Protéines recombinantes ()</term><term>Protéines à fluorescence verte ()</term><term>Récepteurs cytoplasmiques et nucléaires ()</term><term>Souris</term><term>Souris nude</term><term>Séquestosome-1 ()</term><term>Transplantation tumorale</term><term>Tumeurs du poumon (métabolisme)</term><term>Ubiquitine ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Green Fluorescent Proteins</term><term>Microtubule-Associated Proteins</term><term>Nanodiamonds</term><term>Nerve Tissue Proteins</term><term>Nuclear Proteins</term><term>Receptors, Cytoplasmic and Nuclear</term><term>Recombinant Proteins</term><term>Sequestosome-1 Protein</term><term>Ubiquitin</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Lung Neoplasms</term><term>Lysosomes</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Lysosomes</term><term>Tumeurs du poumon</term></keywords><keywords scheme="MESH" xml:lang="en"><term>A549 Cells</term><term>Animals</term><term>Autophagy</term><term>Cell Death</term><term>Cell Line, Tumor</term><term>Cellular Senescence</term><term>Humans</term><term>Mice</term><term>Mice, Nude</term><term>Microscopy, Confocal</term><term>Neoplasm Transplantation</term><term>Protein Binding</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Autophagie</term><term>Cellules A549</term><term>Humains</term><term>Liaison aux protéines</term><term>Lignée cellulaire tumorale</term><term>Microscopie confocale</term><term>Mort cellulaire</term><term>Nanodiamants</term><term>Protéines associées aux microtubules</term><term>Protéines de tissu nerveux</term><term>Protéines nucléaires</term><term>Protéines recombinantes</term><term>Protéines à fluorescence verte</term><term>Récepteurs cytoplasmiques et nucléaires</term><term>Souris</term><term>Souris nude</term><term>Séquestosome-1</term><term>Transplantation tumorale</term><term>Ubiquitine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Selective macroautophagy/autophagy plays a pivotal role in the processing of foreign pathogens and cellular components to maintain homeostasis in human cells. To date, numerous studies have demonstrated the uptake of nanoparticles by cells, but their intracellular processing through selective autophagy remains unclear. Here we show that carbon-based nanodiamonds (NDs) coated with ubiquitin (Ub) bind to autophagy receptors (SQSTM1 [sequestosome 1], OPTN [optineurin], and CALCOCO2/NDP52 [calcium binding and coiled-coil domain 2]) and are then linked to MAP1LC3/LC3 (microtubule-associated protein 1 light chain 3) for entry into the selective autophagy pathway. NDs are ultimately delivered to lysosomes. Ectopically expressed SQSTM1-green fluorescence protein (GFP) could bind to the Ub-coated NDs. By contrast, the Ub-associated domain mutant of SQSTM1 (ΔUBA)-GFP did not bind to the Ub-coated NDs. Chloroquine, an autophagy inhibitor, prevented the ND-containing autophagosomes from fusing with lysosomes. Furthermore, autophagy receptors OPTN and CALCOCO2/NDP52, involved in the processing of bacteria, were found to be involved in the selective autophagy of NDs. However, ND particles located in the lysosomes of cells did not induce mitotic blockage, senescence, or cell death. Single ND clusters in the lysosomes of cells were observed in the xenografted human lung tumors of nude mice. This study demonstrated for the first time that Ub-coated nanoparticles bind to autophagy receptors for entry into the selective autophagy pathway, facilitating their delivery to lysosomes.</div></front></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Liu, Kuang Kai" sort="Liu, Kuang Kai" uniqKey="Liu K" first="Kuang-Kai" last="Liu">Kuang-Kai Liu</name></noRegion><name sortKey="Chang, Chien Jen" sort="Chang, Chien Jen" uniqKey="Chang C" first="Chien-Jen" last="Chang">Chien-Jen Chang</name><name sortKey="Chang, Huan Cheng" sort="Chang, Huan Cheng" uniqKey="Chang H" first="Huan-Cheng" last="Chang">Huan-Cheng Chang</name><name sortKey="Chao, Jui I" sort="Chao, Jui I" uniqKey="Chao J" first="Jui-I" last="Chao">Jui-I Chao</name><name sortKey="Chen, Chinpiao" sort="Chen, Chinpiao" uniqKey="Chen C" first="Chinpiao" last="Chen">Chinpiao Chen</name><name sortKey="Chen, Ting Hua" sort="Chen, Ting Hua" uniqKey="Chen T" first="Ting-Hua" last="Chen">Ting-Hua Chen</name><name sortKey="Huang, Hou Syun" sort="Huang, Hou Syun" uniqKey="Huang H" first="Hou-Syun" last="Huang">Hou-Syun Huang</name><name sortKey="Hwang, Jenn Kang" sort="Hwang, Jenn Kang" uniqKey="Hwang J" first="Jenn-Kang" last="Hwang">Jenn-Kang Hwang</name><name sortKey="Lin, Yu Wei" sort="Lin, Yu Wei" uniqKey="Lin Y" first="Yu-Wei" last="Lin">Yu-Wei Lin</name><name sortKey="Liu, Huei Fang" sort="Liu, Huei Fang" uniqKey="Liu H" first="Huei-Fang" last="Liu">Huei-Fang Liu</name><name sortKey="Naveen Raj, Emmanuel" sort="Naveen Raj, Emmanuel" uniqKey="Naveen Raj E" first="Emmanuel" last="Naveen Raj">Emmanuel Naveen Raj</name><name sortKey="Qiu, Wei Ru" sort="Qiu, Wei Ru" uniqKey="Qiu W" first="Wei-Ru" last="Qiu">Wei-Ru Qiu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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