Absence of the neutrophil serine protease cathepsin G decreases neutrophil granulocyte infiltration but does not change the severity of acute pancreatitis
Identifieur interne : 000B20 ( Main/Exploration ); précédent : 000B19; suivant : 000B21Absence of the neutrophil serine protease cathepsin G decreases neutrophil granulocyte infiltration but does not change the severity of acute pancreatitis
Auteurs : Ali A. Aghdassi [Allemagne] ; Daniel S. John [Allemagne] ; Matthias Sendler [Allemagne] ; Christian Storck [Allemagne] ; Cindy Van Den Brandt [Allemagne] ; Burkhard Krüger [Allemagne] ; Frank Ulrich Weiss [Allemagne] ; Julia Mayerle [Allemagne] ; Markus M. Lerch [Allemagne]Source :
- Scientific Reports [ 2045-2322 ] ; 2019.
Abstract
Acute pancreatitis is characterized by an early intracellular protease activation and invasion of leukocytes into the pancreas. Cathepsins constitute a large group of lysosomal enzymes, that have been shown to modulate trypsinogen activation and neutrophil infiltration. Cathepsin G (CTSG) is a neutrophil serine protease of the chymotrypsin C family known to degrade extracellular matrix components and to have regulatory functions in inflammatory disorders. The aim of this study was to investigate the role of CTSG in pancreatitis. Isolated acinar cells were exposed to recombinant CTSG and supramaximal cholezystokinin stimulation. In CTSG−/− mice and corresponding controls acute experimental pancreatitis was induced by serial caerulein injections. Severity was assessed by histology, serum enzyme levels and zymogen activation. Neutrophil infiltration was quantified by chloro-acetate ersterase staining and myeloperoxidase measurement. CTSG was expessed in inflammatory cells but not in pancreatic acinar cells. CTSG had no effect on intra-acinar-cell trypsinogen activation. In CTSG−/− mice a transient decrease of neutrophil infiltration into the pancreas and lungs was found during acute pancreatitis while the disease severity remained largely unchanged. CTSG is involved in pancreatic neutrophil infiltration during pancreatitis, albeit to a lesser degree than the related neutrophil (PMN) elastase. Its absence therefore leaves pancreatitis severity essentially unaffected.
Url:
DOI: 10.1038/s41598-019-53293-0
PubMed: 31727956
PubMed Central: 6856518
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000194
- to stream Pmc, to step Curation: 000194
- to stream Pmc, to step Checkpoint: 000A44
- to stream Ncbi, to step Merge: 000C10
- to stream Ncbi, to step Curation: 000C10
- to stream Ncbi, to step Checkpoint: 000C10
- to stream Main, to step Merge: 000B20
- to stream Main, to step Curation: 000B20
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Absence of the neutrophil serine protease cathepsin G decreases neutrophil granulocyte infiltration but does not change the severity of acute pancreatitis</title><author><name sortKey="Aghdassi, Ali A" sort="Aghdassi, Ali A" uniqKey="Aghdassi A" first="Ali A." last="Aghdassi">Ali A. Aghdassi</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5603.0</institution-id><institution>Department of Medicine A,</institution><institution>University Medicine Greifswald,</institution></institution-wrap>17475 Greifswald, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>17475 Greifswald</wicri:regionArea></affiliation></author><author><name sortKey="John, Daniel S" sort="John, Daniel S" uniqKey="John D" first="Daniel S." last="John">Daniel S. John</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5603.0</institution-id><institution>Department of Medicine A,</institution><institution>University Medicine Greifswald,</institution></institution-wrap>17475 Greifswald, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>17475 Greifswald</wicri:regionArea></affiliation></author><author><name sortKey="Sendler, Matthias" sort="Sendler, Matthias" uniqKey="Sendler M" first="Matthias" last="Sendler">Matthias Sendler</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5603.0</institution-id><institution>Department of Medicine A,</institution><institution>University Medicine Greifswald,</institution></institution-wrap>17475 Greifswald, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>17475 Greifswald</wicri:regionArea></affiliation></author><author><name sortKey="Storck, Christian" sort="Storck, Christian" uniqKey="Storck C" first="Christian" last="Storck">Christian Storck</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5603.0</institution-id><institution>Department of Medicine A,</institution><institution>University Medicine Greifswald,</institution></institution-wrap>17475 Greifswald, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>17475 Greifswald</wicri:regionArea></affiliation></author><author><name sortKey="Van Den Brandt, Cindy" sort="Van Den Brandt, Cindy" uniqKey="Van Den Brandt C" first="Cindy" last="Van Den Brandt">Cindy Van Den Brandt</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5603.0</institution-id><institution>Department of Medicine A,</institution><institution>University Medicine Greifswald,</institution></institution-wrap>17475 Greifswald, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>17475 Greifswald</wicri:regionArea></affiliation></author><author><name sortKey="Kruger, Burkhard" sort="Kruger, Burkhard" uniqKey="Kruger B" first="Burkhard" last="Krüger">Burkhard Krüger</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000000121858338</institution-id><institution-id institution-id-type="GRID">grid.10493.3f</institution-id><institution>Division of Medical Biology,</institution><institution>University of Rostock,</institution></institution-wrap>18051 Rostock, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>18051 Rostock</wicri:regionArea></affiliation></author><author><name sortKey="Weiss, Frank Ulrich" sort="Weiss, Frank Ulrich" uniqKey="Weiss F" first="Frank Ulrich" last="Weiss">Frank Ulrich Weiss</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5603.0</institution-id><institution>Department of Medicine A,</institution><institution>University Medicine Greifswald,</institution></institution-wrap>17475 Greifswald, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>17475 Greifswald</wicri:regionArea></affiliation></author><author><name sortKey="Mayerle, Julia" sort="Mayerle, Julia" uniqKey="Mayerle J" first="Julia" last="Mayerle">Julia Mayerle</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1936 973X</institution-id><institution-id institution-id-type="GRID">grid.5252.0</institution-id><institution>Department of Medicine II,</institution><institution>Ludwigs-Maximilians University Munich,</institution></institution-wrap>80539 Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>80539 Munich</wicri:regionArea></affiliation></author><author><name sortKey="Lerch, Markus M" sort="Lerch, Markus M" uniqKey="Lerch M" first="Markus M." last="Lerch">Markus M. Lerch</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5603.0</institution-id><institution>Department of Medicine A,</institution><institution>University Medicine Greifswald,</institution></institution-wrap>17475 Greifswald, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>17475 Greifswald</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31727956</idno><idno type="pmc">6856518</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6856518</idno><idno type="RBID">PMC:6856518</idno><idno type="doi">10.1038/s41598-019-53293-0</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000194</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000194</idno><idno type="wicri:Area/Pmc/Curation">000194</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000194</idno><idno type="wicri:Area/Pmc/Checkpoint">000A44</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000A44</idno><idno type="wicri:Area/Ncbi/Merge">000C10</idno><idno type="wicri:Area/Ncbi/Curation">000C10</idno><idno type="wicri:Area/Ncbi/Checkpoint">000C10</idno><idno type="wicri:Area/Main/Merge">000B20</idno><idno type="wicri:Area/Main/Curation">000B20</idno><idno type="wicri:Area/Main/Exploration">000B20</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Absence of the neutrophil serine protease cathepsin G decreases neutrophil granulocyte infiltration but does not change the severity of acute pancreatitis</title><author><name sortKey="Aghdassi, Ali A" sort="Aghdassi, Ali A" uniqKey="Aghdassi A" first="Ali A." last="Aghdassi">Ali A. Aghdassi</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5603.0</institution-id><institution>Department of Medicine A,</institution><institution>University Medicine Greifswald,</institution></institution-wrap>17475 Greifswald, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>17475 Greifswald</wicri:regionArea></affiliation></author><author><name sortKey="John, Daniel S" sort="John, Daniel S" uniqKey="John D" first="Daniel S." last="John">Daniel S. John</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5603.0</institution-id><institution>Department of Medicine A,</institution><institution>University Medicine Greifswald,</institution></institution-wrap>17475 Greifswald, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>17475 Greifswald</wicri:regionArea></affiliation></author><author><name sortKey="Sendler, Matthias" sort="Sendler, Matthias" uniqKey="Sendler M" first="Matthias" last="Sendler">Matthias Sendler</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5603.0</institution-id><institution>Department of Medicine A,</institution><institution>University Medicine Greifswald,</institution></institution-wrap>17475 Greifswald, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>17475 Greifswald</wicri:regionArea></affiliation></author><author><name sortKey="Storck, Christian" sort="Storck, Christian" uniqKey="Storck C" first="Christian" last="Storck">Christian Storck</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5603.0</institution-id><institution>Department of Medicine A,</institution><institution>University Medicine Greifswald,</institution></institution-wrap>17475 Greifswald, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>17475 Greifswald</wicri:regionArea></affiliation></author><author><name sortKey="Van Den Brandt, Cindy" sort="Van Den Brandt, Cindy" uniqKey="Van Den Brandt C" first="Cindy" last="Van Den Brandt">Cindy Van Den Brandt</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5603.0</institution-id><institution>Department of Medicine A,</institution><institution>University Medicine Greifswald,</institution></institution-wrap>17475 Greifswald, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>17475 Greifswald</wicri:regionArea></affiliation></author><author><name sortKey="Kruger, Burkhard" sort="Kruger, Burkhard" uniqKey="Kruger B" first="Burkhard" last="Krüger">Burkhard Krüger</name><affiliation wicri:level="1"><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="ISNI">0000000121858338</institution-id><institution-id institution-id-type="GRID">grid.10493.3f</institution-id><institution>Division of Medical Biology,</institution><institution>University of Rostock,</institution></institution-wrap>18051 Rostock, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>18051 Rostock</wicri:regionArea></affiliation></author><author><name sortKey="Weiss, Frank Ulrich" sort="Weiss, Frank Ulrich" uniqKey="Weiss F" first="Frank Ulrich" last="Weiss">Frank Ulrich Weiss</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5603.0</institution-id><institution>Department of Medicine A,</institution><institution>University Medicine Greifswald,</institution></institution-wrap>17475 Greifswald, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>17475 Greifswald</wicri:regionArea></affiliation></author><author><name sortKey="Mayerle, Julia" sort="Mayerle, Julia" uniqKey="Mayerle J" first="Julia" last="Mayerle">Julia Mayerle</name><affiliation wicri:level="1"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="ISNI">0000 0004 1936 973X</institution-id><institution-id institution-id-type="GRID">grid.5252.0</institution-id><institution>Department of Medicine II,</institution><institution>Ludwigs-Maximilians University Munich,</institution></institution-wrap>80539 Munich, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>80539 Munich</wicri:regionArea></affiliation></author><author><name sortKey="Lerch, Markus M" sort="Lerch, Markus M" uniqKey="Lerch M" first="Markus M." last="Lerch">Markus M. Lerch</name><affiliation wicri:level="1"><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.5603.0</institution-id><institution>Department of Medicine A,</institution><institution>University Medicine Greifswald,</institution></institution-wrap>17475 Greifswald, Germany</nlm:aff><country xml:lang="fr">Allemagne</country><wicri:regionArea>17475 Greifswald</wicri:regionArea></affiliation></author></analytic><series><title level="j">Scientific Reports</title><idno type="eISSN">2045-2322</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="Par1">Acute pancreatitis is characterized by an early intracellular protease activation and invasion of leukocytes into the pancreas. Cathepsins constitute a large group of lysosomal enzymes, that have been shown to modulate trypsinogen activation and neutrophil infiltration. Cathepsin G (CTSG) is a neutrophil serine protease of the chymotrypsin C family known to degrade extracellular matrix components and to have regulatory functions in inflammatory disorders. The aim of this study was to investigate the role of CTSG in pancreatitis. Isolated acinar cells were exposed to recombinant CTSG and supramaximal cholezystokinin stimulation. In CTSG<sup>−/−</sup> mice and corresponding controls acute experimental pancreatitis was induced by serial caerulein injections. Severity was assessed by histology, serum enzyme levels and zymogen activation. Neutrophil infiltration was quantified by chloro-acetate ersterase staining and myeloperoxidase measurement. CTSG was expessed in inflammatory cells but not in pancreatic acinar cells. CTSG had no effect on intra-acinar-cell trypsinogen activation. In CTSG<sup>−/−</sup> mice a transient decrease of neutrophil infiltration into the pancreas and lungs was found during acute pancreatitis while the disease severity remained largely unchanged. CTSG is involved in pancreatic neutrophil infiltration during pancreatitis, albeit to a lesser degree than the related neutrophil (PMN) elastase. Its absence therefore leaves pancreatitis severity essentially unaffected.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Peery, Af" uniqKey="Peery A">AF Peery</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chronic Pancreatitis German Society Of, D" uniqKey="Chronic Pancreatitis German Society Of D">D Chronic Pancreatitis German Society of</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sendler, M" uniqKey="Sendler M">M Sendler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hofbauer, B" uniqKey="Hofbauer B">B Hofbauer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Halangk, W" uniqKey="Halangk W">W Halangk</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aghdassi, Aa" uniqKey="Aghdassi A">AA Aghdassi</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wartmann, T" uniqKey="Wartmann T">T Wartmann</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="John, Ds" uniqKey="John D">DS John</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Korkmaz, B" uniqKey="Korkmaz B">B Korkmaz</name></author><author><name sortKey="Moreau, T" uniqKey="Moreau T">T Moreau</name></author><author><name sortKey="Gauthier, F" uniqKey="Gauthier F">F Gauthier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bank, U" uniqKey="Bank U">U Bank</name></author><author><name sortKey="Ansorge, S" uniqKey="Ansorge S">S Ansorge</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Von Dobschuetz, E" uniqKey="Von Dobschuetz E">E von Dobschuetz</name></author><author><name sortKey="Hoffmann, T" uniqKey="Hoffmann T">T Hoffmann</name></author><author><name sortKey="Messmer, K" uniqKey="Messmer K">K Messmer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Leppkes, M" uniqKey="Leppkes M">M Leppkes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Heutinck, Km" uniqKey="Heutinck K">KM Heutinck</name></author><author><name sortKey="Ten Berge, Ij" uniqKey="Ten Berge I">IJ ten Berge</name></author><author><name sortKey="Hack, Ce" uniqKey="Hack C">CE Hack</name></author><author><name sortKey="Hamann, J" uniqKey="Hamann J">J Hamann</name></author><author><name sortKey="Rowshani, At" uniqKey="Rowshani A">AT Rowshani</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gukovskaya, As" uniqKey="Gukovskaya A">AS Gukovskaya</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Rinderknecht, H" uniqKey="Rinderknecht H">H Rinderknecht</name></author><author><name sortKey="Engeling, Er" uniqKey="Engeling E">ER Engeling</name></author><author><name sortKey="Bunnell, Mj" uniqKey="Bunnell M">MJ Bunnell</name></author><author><name sortKey="Geokas, Mc" uniqKey="Geokas M">MC Geokas</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lerch, Mm" uniqKey="Lerch M">MM Lerch</name></author><author><name sortKey="Saluja, Ak" uniqKey="Saluja A">AK Saluja</name></author><author><name sortKey="Dawra, R" uniqKey="Dawra R">R Dawra</name></author><author><name sortKey="Saluja, M" uniqKey="Saluja M">M Saluja</name></author><author><name sortKey="Steer, Ml" uniqKey="Steer M">ML Steer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sendler, M" uniqKey="Sendler M">M Sendler</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Schnekenburger, J" uniqKey="Schnekenburger J">J Schnekenburger</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Perides, G" uniqKey="Perides G">G Perides</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hamon, Y" uniqKey="Hamon Y">Y Hamon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wiedow, O" uniqKey="Wiedow O">O Wiedow</name></author><author><name sortKey="Meyer Hoffert, U" uniqKey="Meyer Hoffert U">U Meyer-Hoffert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Sambrano, Gr" uniqKey="Sambrano G">GR Sambrano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mayerle, J" uniqKey="Mayerle J">J Mayerle</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hermant, B" uniqKey="Hermant B">B Hermant</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gordon, Jr" uniqKey="Gordon J">JR Gordon</name></author><author><name sortKey="Galli, Sj" uniqKey="Galli S">SJ Galli</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Molgora, M" uniqKey="Molgora M">M Molgora</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Mortha, A" uniqKey="Mortha A">A Mortha</name></author><author><name sortKey="Burrows, K" uniqKey="Burrows K">K Burrows</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tkalcevic, J" uniqKey="Tkalcevic J">J Tkalcevic</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Steinwede, K" uniqKey="Steinwede K">K Steinwede</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wilson, Tj" uniqKey="Wilson T">TJ Wilson</name></author><author><name sortKey="Nannuru, Kc" uniqKey="Nannuru K">KC Nannuru</name></author><author><name sortKey="Futakuchi, M" uniqKey="Futakuchi M">M Futakuchi</name></author><author><name sortKey="Sadanandam, A" uniqKey="Sadanandam A">A Sadanandam</name></author><author><name sortKey="Singh, Rk" uniqKey="Singh R">RK Singh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lerch, Mm" uniqKey="Lerch M">MM Lerch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Halangk, W" uniqKey="Halangk W">W Halangk</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Niederau, C" uniqKey="Niederau C">C Niederau</name></author><author><name sortKey="Ferrell, Ld" uniqKey="Ferrell L">LD Ferrell</name></author><author><name sortKey="Grendell, Jh" uniqKey="Grendell J">JH Grendell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Essin, K" uniqKey="Essin K">K Essin</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Allemagne</li></country></list><tree><country name="Allemagne"><noRegion><name sortKey="Aghdassi, Ali A" sort="Aghdassi, Ali A" uniqKey="Aghdassi A" first="Ali A." last="Aghdassi">Ali A. Aghdassi</name></noRegion><name sortKey="John, Daniel S" sort="John, Daniel S" uniqKey="John D" first="Daniel S." last="John">Daniel S. John</name><name sortKey="Kruger, Burkhard" sort="Kruger, Burkhard" uniqKey="Kruger B" first="Burkhard" last="Krüger">Burkhard Krüger</name><name sortKey="Lerch, Markus M" sort="Lerch, Markus M" uniqKey="Lerch M" first="Markus M." last="Lerch">Markus M. Lerch</name><name sortKey="Mayerle, Julia" sort="Mayerle, Julia" uniqKey="Mayerle J" first="Julia" last="Mayerle">Julia Mayerle</name><name sortKey="Sendler, Matthias" sort="Sendler, Matthias" uniqKey="Sendler M" first="Matthias" last="Sendler">Matthias Sendler</name><name sortKey="Storck, Christian" sort="Storck, Christian" uniqKey="Storck C" first="Christian" last="Storck">Christian Storck</name><name sortKey="Van Den Brandt, Cindy" sort="Van Den Brandt, Cindy" uniqKey="Van Den Brandt C" first="Cindy" last="Van Den Brandt">Cindy Van Den Brandt</name><name sortKey="Weiss, Frank Ulrich" sort="Weiss, Frank Ulrich" uniqKey="Weiss F" first="Frank Ulrich" last="Weiss">Frank Ulrich Weiss</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000B20 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000B20 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:6856518
|texte= Absence of the neutrophil serine protease cathepsin G decreases neutrophil granulocyte infiltration but does not change the severity of acute pancreatitis
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31727956" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ChloroquineV1
| This area was generated with Dilib version V0.6.33. |  |