Profiling of Polar Metabolites in Mouse Feces Using Four Analytical Platforms to Study the Effects Of Cathelicidin-Related Antimicrobial Peptide in Alcoholic Liver Disease
Identifieur interne : 000517 ( Main/Exploration ); précédent : 000516; suivant : 000518Profiling of Polar Metabolites in Mouse Feces Using Four Analytical Platforms to Study the Effects Of Cathelicidin-Related Antimicrobial Peptide in Alcoholic Liver Disease
Auteurs : Liqing He [États-Unis] ; Fengyuan Li [États-Unis] ; Xinmin Yin [États-Unis] ; Patrick Bohman [États-Unis] ; Seongho Kim [États-Unis] ; Craig J. Mcclain [États-Unis] ; Wenke Feng [États-Unis] ; Xiang Zhang [États-Unis]Source :
- Journal of proteome research [ 1535-3893 ] ; 2019.
Abstract
Alterations in gut bacterial homeostasis result in changes in intestinal metabolites. To investigate the effects of alcohol on fecal metabolites and the role of cathelicidin-related antimicrobial peptide (CRAMP) in alcoholic liver disease (ALD), CRAMP knockout (KO) and their control wild type (WT) mice were fed a Lieber-DeCarli liquid diet with or without alcohol. Polar metabolites in mouse feces were analyzed by GC × GC-MS and 2DLC-MS, and the concentrations of short chain fatty acids (SCFAs) were measured by GC-MS. A total of 95 and 190 metabolites were detected by GC × GC-MS and 2DLC-MS, respectively. Among the significantly changed metabolites, taurine and nicotinic acid were decreased in WT mice fed alcohol, which were also down-regulated in KO mice fed without alcohol. Interestingly, these two metabolites were increased in KO mice fed alcohol compared to them in WT controls. Additionally, SCFAs were significantly decreased in WT mice fed alcohol and in KO mice fed without alcohol, whereas two branched-chain SCFAs were increased by alcohol treatment in KO mice. In summary, the analytical platforms employed in this study successfully dissected the alterations of polar metabolites and SCFAs in fecal samples, which helped understand the effects of alcohol consumption and CRAMP in intestinal metabolism and alcohol-induced liver injury.
Url:
DOI: 10.1021/acs.jproteome.9b00181
PubMed: 31188604
PubMed Central: 6748645
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: 000380
- to stream Pmc, to step Curation: 000380
- to stream Pmc, to step Checkpoint: 000472
- to stream Ncbi, to step Merge: 000693
- to stream Ncbi, to step Curation: 000693
- to stream Ncbi, to step Checkpoint: 000693
- to stream Main, to step Merge: 000517
- to stream Main, to step Curation: 000517
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Profiling of Polar Metabolites in Mouse Feces Using Four Analytical Platforms to Study the Effects Of Cathelicidin-Related Antimicrobial Peptide in Alcoholic Liver Disease</title><author><name sortKey="He, Liqing" sort="He, Liqing" uniqKey="He L" first="Liqing" last="He">Liqing He</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Chemistry, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A2">University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A3">University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A4">Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation></author><author><name sortKey="Li, Fengyuan" sort="Li, Fengyuan" uniqKey="Li F" first="Fengyuan" last="Li">Fengyuan Li</name><affiliation wicri:level="1"><nlm:aff id="A3">University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A4">Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A5">Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation></author><author><name sortKey="Yin, Xinmin" sort="Yin, Xinmin" uniqKey="Yin X" first="Xinmin" last="Yin">Xinmin Yin</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Chemistry, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A2">University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A3">University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A4">Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation></author><author><name sortKey="Bohman, Patrick" sort="Bohman, Patrick" uniqKey="Bohman P" first="Patrick" last="Bohman">Patrick Bohman</name><affiliation wicri:level="1"><nlm:aff id="A8">Thermo Fisher Scientific International Inc., 3000 Lakeside Dr., Bannockburn, Illinois 60015, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Thermo Fisher Scientific International Inc., 3000 Lakeside Dr., Bannockburn, Illinois 60015</wicri:regionArea><wicri:noRegion>Illinois 60015</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Seongho" sort="Kim, Seongho" uniqKey="Kim S" first="Seongho" last="Kim">Seongho Kim</name><affiliation wicri:level="1"><nlm:aff id="A9">Biostatistics Core, Karmanos Cancer Institute, Wayne State University, Detroit, Michigan 48201, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biostatistics Core, Karmanos Cancer Institute, Wayne State University, Detroit, Michigan 48201</wicri:regionArea><wicri:noRegion>Michigan 48201</wicri:noRegion></affiliation></author><author><name sortKey="Mcclain, Craig J" sort="Mcclain, Craig J" uniqKey="Mcclain C" first="Craig J." last="Mcclain">Craig J. Mcclain</name><affiliation wicri:level="1"><nlm:aff id="A2">University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A3">University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A5">Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A6">Department of Medicine, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A7">Robley Rex Louisville VAMC, Louisville, Kentucky 40292, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Robley Rex Louisville VAMC, Louisville, Kentucky 40292</wicri:regionArea><wicri:noRegion>Kentucky 40292</wicri:noRegion></affiliation></author><author><name sortKey="Feng, Wenke" sort="Feng, Wenke" uniqKey="Feng W" first="Wenke" last="Feng">Wenke Feng</name><affiliation wicri:level="1"><nlm:aff id="A2">University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A3">University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A4">Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A5">Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A6">Department of Medicine, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Xiang" sort="Zhang, Xiang" uniqKey="Zhang X" first="Xiang" last="Zhang">Xiang Zhang</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Chemistry, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A2">University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A3">University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A4">Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A5">Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">31188604</idno><idno type="pmc">6748645</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6748645</idno><idno type="RBID">PMC:6748645</idno><idno type="doi">10.1021/acs.jproteome.9b00181</idno><date when="2019">2019</date><idno type="wicri:Area/Pmc/Corpus">000380</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000380</idno><idno type="wicri:Area/Pmc/Curation">000380</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000380</idno><idno type="wicri:Area/Pmc/Checkpoint">000472</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000472</idno><idno type="wicri:Area/Ncbi/Merge">000693</idno><idno type="wicri:Area/Ncbi/Curation">000693</idno><idno type="wicri:Area/Ncbi/Checkpoint">000693</idno><idno type="wicri:doubleKey">1535-3893:2019:He L:profiling:of:polar</idno><idno type="wicri:Area/Main/Merge">000517</idno><idno type="wicri:Area/Main/Curation">000517</idno><idno type="wicri:Area/Main/Exploration">000517</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Profiling of Polar Metabolites in Mouse Feces Using Four Analytical Platforms to Study the Effects Of Cathelicidin-Related Antimicrobial Peptide in Alcoholic Liver Disease</title><author><name sortKey="He, Liqing" sort="He, Liqing" uniqKey="He L" first="Liqing" last="He">Liqing He</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Chemistry, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A2">University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A3">University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A4">Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation></author><author><name sortKey="Li, Fengyuan" sort="Li, Fengyuan" uniqKey="Li F" first="Fengyuan" last="Li">Fengyuan Li</name><affiliation wicri:level="1"><nlm:aff id="A3">University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A4">Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A5">Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation></author><author><name sortKey="Yin, Xinmin" sort="Yin, Xinmin" uniqKey="Yin X" first="Xinmin" last="Yin">Xinmin Yin</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Chemistry, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A2">University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A3">University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A4">Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation></author><author><name sortKey="Bohman, Patrick" sort="Bohman, Patrick" uniqKey="Bohman P" first="Patrick" last="Bohman">Patrick Bohman</name><affiliation wicri:level="1"><nlm:aff id="A8">Thermo Fisher Scientific International Inc., 3000 Lakeside Dr., Bannockburn, Illinois 60015, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Thermo Fisher Scientific International Inc., 3000 Lakeside Dr., Bannockburn, Illinois 60015</wicri:regionArea><wicri:noRegion>Illinois 60015</wicri:noRegion></affiliation></author><author><name sortKey="Kim, Seongho" sort="Kim, Seongho" uniqKey="Kim S" first="Seongho" last="Kim">Seongho Kim</name><affiliation wicri:level="1"><nlm:aff id="A9">Biostatistics Core, Karmanos Cancer Institute, Wayne State University, Detroit, Michigan 48201, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Biostatistics Core, Karmanos Cancer Institute, Wayne State University, Detroit, Michigan 48201</wicri:regionArea><wicri:noRegion>Michigan 48201</wicri:noRegion></affiliation></author><author><name sortKey="Mcclain, Craig J" sort="Mcclain, Craig J" uniqKey="Mcclain C" first="Craig J." last="Mcclain">Craig J. Mcclain</name><affiliation wicri:level="1"><nlm:aff id="A2">University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A3">University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A5">Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A6">Department of Medicine, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A7">Robley Rex Louisville VAMC, Louisville, Kentucky 40292, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Robley Rex Louisville VAMC, Louisville, Kentucky 40292</wicri:regionArea><wicri:noRegion>Kentucky 40292</wicri:noRegion></affiliation></author><author><name sortKey="Feng, Wenke" sort="Feng, Wenke" uniqKey="Feng W" first="Wenke" last="Feng">Wenke Feng</name><affiliation wicri:level="1"><nlm:aff id="A2">University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A3">University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A4">Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A5">Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A6">Department of Medicine, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation></author><author><name sortKey="Zhang, Xiang" sort="Zhang, Xiang" uniqKey="Zhang X" first="Xiang" last="Zhang">Xiang Zhang</name><affiliation wicri:level="1"><nlm:aff id="A1">Department of Chemistry, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Chemistry, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A2">University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Alcohol Research Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A3">University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>University of Louisville Hepatobiology & Toxicology Center, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A4">Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="A5">Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208, United States</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Pharmacology & Toxicology, University of Louisville, Louisville, Kentucky 40208</wicri:regionArea><wicri:noRegion>Kentucky 40208</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of proteome research</title><idno type="ISSN">1535-3893</idno><idno type="eISSN">1535-3907</idno><imprint><date when="2019">2019</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p id="P1">Alterations in gut bacterial homeostasis result in changes in intestinal metabolites. To investigate the effects of alcohol on fecal metabolites and the role of cathelicidin-related antimicrobial peptide (CRAMP) in alcoholic liver disease (ALD), CRAMP knockout (KO) and their control wild type (WT) mice were fed a Lieber-DeCarli liquid diet with or without alcohol. Polar metabolites in mouse feces were analyzed by GC × GC-MS and 2DLC-MS, and the concentrations of short chain fatty acids (SCFAs) were measured by GC-MS. A total of 95 and 190 metabolites were detected by GC × GC-MS and 2DLC-MS, respectively. Among the significantly changed metabolites, taurine and nicotinic acid were decreased in WT mice fed alcohol, which were also down-regulated in KO mice fed without alcohol. Interestingly, these two metabolites were increased in KO mice fed alcohol compared to them in WT controls. Additionally, SCFAs were significantly decreased in WT mice fed alcohol and in KO mice fed without alcohol, whereas two branched-chain SCFAs were increased by alcohol treatment in KO mice. In summary, the analytical platforms employed in this study successfully dissected the alterations of polar metabolites and SCFAs in fecal samples, which helped understand the effects of alcohol consumption and CRAMP in intestinal metabolism and alcohol-induced liver injury.</p></div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="He, Liqing" sort="He, Liqing" uniqKey="He L" first="Liqing" last="He">Liqing He</name></noRegion><name sortKey="Bohman, Patrick" sort="Bohman, Patrick" uniqKey="Bohman P" first="Patrick" last="Bohman">Patrick Bohman</name><name sortKey="Feng, Wenke" sort="Feng, Wenke" uniqKey="Feng W" first="Wenke" last="Feng">Wenke Feng</name><name sortKey="Feng, Wenke" sort="Feng, Wenke" uniqKey="Feng W" first="Wenke" last="Feng">Wenke Feng</name><name sortKey="Feng, Wenke" sort="Feng, Wenke" uniqKey="Feng W" first="Wenke" last="Feng">Wenke Feng</name><name sortKey="Feng, Wenke" sort="Feng, Wenke" uniqKey="Feng W" first="Wenke" last="Feng">Wenke Feng</name><name sortKey="Feng, Wenke" sort="Feng, Wenke" uniqKey="Feng W" first="Wenke" last="Feng">Wenke Feng</name><name sortKey="He, Liqing" sort="He, Liqing" uniqKey="He L" first="Liqing" last="He">Liqing He</name><name sortKey="He, Liqing" sort="He, Liqing" uniqKey="He L" first="Liqing" last="He">Liqing He</name><name sortKey="He, Liqing" sort="He, Liqing" uniqKey="He L" first="Liqing" last="He">Liqing He</name><name sortKey="Kim, Seongho" sort="Kim, Seongho" uniqKey="Kim S" first="Seongho" last="Kim">Seongho Kim</name><name sortKey="Li, Fengyuan" sort="Li, Fengyuan" uniqKey="Li F" first="Fengyuan" last="Li">Fengyuan Li</name><name sortKey="Li, Fengyuan" sort="Li, Fengyuan" uniqKey="Li F" first="Fengyuan" last="Li">Fengyuan Li</name><name sortKey="Li, Fengyuan" sort="Li, Fengyuan" uniqKey="Li F" first="Fengyuan" last="Li">Fengyuan Li</name><name sortKey="Mcclain, Craig J" sort="Mcclain, Craig J" uniqKey="Mcclain C" first="Craig J." last="Mcclain">Craig J. Mcclain</name><name sortKey="Mcclain, Craig J" sort="Mcclain, Craig J" uniqKey="Mcclain C" first="Craig J." last="Mcclain">Craig J. Mcclain</name><name sortKey="Mcclain, Craig J" sort="Mcclain, Craig J" uniqKey="Mcclain C" first="Craig J." last="Mcclain">Craig J. Mcclain</name><name sortKey="Mcclain, Craig J" sort="Mcclain, Craig J" uniqKey="Mcclain C" first="Craig J." last="Mcclain">Craig J. Mcclain</name><name sortKey="Mcclain, Craig J" sort="Mcclain, Craig J" uniqKey="Mcclain C" first="Craig J." last="Mcclain">Craig J. Mcclain</name><name sortKey="Yin, Xinmin" sort="Yin, Xinmin" uniqKey="Yin X" first="Xinmin" last="Yin">Xinmin Yin</name><name sortKey="Yin, Xinmin" sort="Yin, Xinmin" uniqKey="Yin X" first="Xinmin" last="Yin">Xinmin Yin</name><name sortKey="Yin, Xinmin" sort="Yin, Xinmin" uniqKey="Yin X" first="Xinmin" last="Yin">Xinmin Yin</name><name sortKey="Yin, Xinmin" sort="Yin, Xinmin" uniqKey="Yin X" first="Xinmin" last="Yin">Xinmin Yin</name><name sortKey="Zhang, Xiang" sort="Zhang, Xiang" uniqKey="Zhang X" first="Xiang" last="Zhang">Xiang Zhang</name><name sortKey="Zhang, Xiang" sort="Zhang, Xiang" uniqKey="Zhang X" first="Xiang" last="Zhang">Xiang Zhang</name><name sortKey="Zhang, Xiang" sort="Zhang, Xiang" uniqKey="Zhang X" first="Xiang" last="Zhang">Xiang Zhang</name><name sortKey="Zhang, Xiang" sort="Zhang, Xiang" uniqKey="Zhang X" first="Xiang" last="Zhang">Xiang Zhang</name><name sortKey="Zhang, Xiang" sort="Zhang, Xiang" uniqKey="Zhang X" first="Xiang" last="Zhang">Xiang Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000517 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000517 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= PMC:6748645
|texte= Profiling of Polar Metabolites in Mouse Feces Using Four Analytical Platforms to Study the Effects Of Cathelicidin-Related Antimicrobial Peptide in Alcoholic Liver Disease
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:31188604" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a ChloroquineV1
| This area was generated with Dilib version V0.6.33. |  |