Site-specific stable insertion into the human cytomegalovirus genome of a foreign gene under control of the SV40 promoter
Identifieur interne : 001D17 ( Istex/Curation ); précédent : 001D16; suivant : 001D18Site-specific stable insertion into the human cytomegalovirus genome of a foreign gene under control of the SV40 promoter
Auteurs : Takekoshi Masataka [Japon] ; Maeda-Takekoshi Fumiko [Japon] ; Ihara Seiji [Japon] ; Sakuma Sadatoshi ; Watanabe Yasushi [Japon]Source :
- Gene [ 0378-1119 ] ; 1991.
English descriptors
- Teeft :
- Bgal, Bgal activity, Blue plaques, Chimeric, Chimeric gene, Chimeric iucz gene, Cytomegalovirus, Early stage, Foreign gene, Foreign genes, Fragment, Gene, Genome, Genome structures, Hcmv, Hcmv genome, Hcmvlacr, Iacz gene, Insertion, Iucz, Iucz gene, Late glycoprotein, Late stages, Marker genes, Mocarski, Negative regulation, Open circles, Opposite directions, Plasmid, Progeny genome, Promoter, Recombinant, Recombinant genomes, Recombinant virus, Recombinant viruses, Reversion rate, Simian virus, Viral, Viral proteins, Virion dnas, Virus replication, Virus vector.
Abstract
Abstract: On the basis of a previous finding that the 7.8-kb HindIII-O fragment of the human cytomegalovirus strain Towne genome is nonessential for viral replication, we constructed a vector, pKM, that directs introduction of foreign genes by homologous recombination precisely replacing the O fragment. Using this vector, we constructed Towne-strain-derived recombinant viruses in which a chimeric lacZ gene fused to the simian virus 40 promoter and a poly(A) signal were inserted in place of the O fragment. Two types of recombinants were obtained which carried the chimeric gene in opposite directions. β-Galacto- sidase (βGal) was produced throughout the infection cycle in human embryonic lung cells infected with these recombinants, and the rate of its synthesis in the early stages of infection was comparable to that of synthesis of a 65-kDa viral glycoprotein, one of the abundantly produced viral proteins. The chimeric lacZ gene introduced was stable and no lacZ− revertants have been observed so far.
Url:
DOI: 10.1016/0378-1119(91)90413-6
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001D17
Links to Exploration step
ISTEX:921795161D969F4C358BC51E4BF2CCDB6D35DFEACuration
No country items
Sakuma Sadatoshi<affiliation><mods:affiliation>Molecular Oncology Division, Meiji Institute of Health Science, 540 Naruda, Odawara 250 Japan Tel. (0465)37-3661</mods:affiliation><wicri:noCountry code="subField">(0465)37-3661</wicri:noCountry></affiliation>Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Site-specific stable insertion into the human cytomegalovirus genome of a foreign gene under control of the SV40 promoter</title><author><name sortKey="Masataka, Takekoshi" sort="Masataka, Takekoshi" uniqKey="Masataka T" first="Takekoshi" last="Masataka">Takekoshi Masataka</name><affiliation wicri:level="1"><mods:affiliation>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai, Isehara 259-11 Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai</wicri:regionArea></affiliation></author><author><name sortKey="Fumiko, Maeda Takekoshi" sort="Fumiko, Maeda Takekoshi" uniqKey="Fumiko M" first="Maeda-Takekoshi" last="Fumiko">Maeda-Takekoshi Fumiko</name><affiliation wicri:level="1"><mods:affiliation>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai, Isehara 259-11 Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai</wicri:regionArea></affiliation></author><author><name sortKey="Seiji, Ihara" sort="Seiji, Ihara" uniqKey="Seiji I" first="Ihara" last="Seiji">Ihara Seiji</name><affiliation wicri:level="1"><mods:affiliation>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai, Isehara 259-11 Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai</wicri:regionArea></affiliation></author><author><name sortKey="Sadatoshi, Sakuma" sort="Sadatoshi, Sakuma" uniqKey="Sadatoshi S" first="Sakuma" last="Sadatoshi">Sakuma Sadatoshi</name><affiliation><mods:affiliation>Molecular Oncology Division, Meiji Institute of Health Science, 540 Naruda, Odawara 250 Japan Tel. (0465)37-3661</mods:affiliation><wicri:noCountry code="subField">(0465)37-3661</wicri:noCountry></affiliation></author><author><name sortKey="Yasushi, Watanabe" sort="Yasushi, Watanabe" uniqKey="Yasushi W" first="Watanabe" last="Yasushi">Watanabe Yasushi</name><affiliation wicri:level="1"><mods:affiliation>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai, Isehara 259-11 Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:921795161D969F4C358BC51E4BF2CCDB6D35DFEA</idno><date when="1991" year="1991">1991</date><idno type="doi">10.1016/0378-1119(91)90413-6</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-28681FFT-1/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001D17</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001D17</idno><idno type="wicri:Area/Istex/Curation">001D17</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Site-specific stable insertion into the human cytomegalovirus genome of a foreign gene under control of the SV40 promoter</title><author><name sortKey="Masataka, Takekoshi" sort="Masataka, Takekoshi" uniqKey="Masataka T" first="Takekoshi" last="Masataka">Takekoshi Masataka</name><affiliation wicri:level="1"><mods:affiliation>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai, Isehara 259-11 Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai</wicri:regionArea></affiliation></author><author><name sortKey="Fumiko, Maeda Takekoshi" sort="Fumiko, Maeda Takekoshi" uniqKey="Fumiko M" first="Maeda-Takekoshi" last="Fumiko">Maeda-Takekoshi Fumiko</name><affiliation wicri:level="1"><mods:affiliation>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai, Isehara 259-11 Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai</wicri:regionArea></affiliation></author><author><name sortKey="Seiji, Ihara" sort="Seiji, Ihara" uniqKey="Seiji I" first="Ihara" last="Seiji">Ihara Seiji</name><affiliation wicri:level="1"><mods:affiliation>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai, Isehara 259-11 Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai</wicri:regionArea></affiliation></author><author><name sortKey="Sadatoshi, Sakuma" sort="Sadatoshi, Sakuma" uniqKey="Sadatoshi S" first="Sakuma" last="Sadatoshi">Sakuma Sadatoshi</name><affiliation><mods:affiliation>Molecular Oncology Division, Meiji Institute of Health Science, 540 Naruda, Odawara 250 Japan Tel. (0465)37-3661</mods:affiliation><wicri:noCountry code="subField">(0465)37-3661</wicri:noCountry></affiliation></author><author><name sortKey="Yasushi, Watanabe" sort="Yasushi, Watanabe" uniqKey="Yasushi W" first="Watanabe" last="Yasushi">Watanabe Yasushi</name><affiliation wicri:level="1"><mods:affiliation>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai, Isehara 259-11 Japan</mods:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Department of Molecular Biology, Tokai University School of Medicine, Bohseidai</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Gene</title><title level="j" type="abbrev">GENE</title><idno type="ISSN">0378-1119</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1991">1991</date><biblScope unit="volume">101</biblScope><biblScope unit="issue">2</biblScope><biblScope unit="page" from="209">209</biblScope><biblScope unit="page" to="213">213</biblScope></imprint><idno type="ISSN">0378-1119</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0378-1119</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Bgal</term><term>Bgal activity</term><term>Blue plaques</term><term>Chimeric</term><term>Chimeric gene</term><term>Chimeric iucz gene</term><term>Cytomegalovirus</term><term>Early stage</term><term>Foreign gene</term><term>Foreign genes</term><term>Fragment</term><term>Gene</term><term>Genome</term><term>Genome structures</term><term>Hcmv</term><term>Hcmv genome</term><term>Hcmvlacr</term><term>Iacz gene</term><term>Insertion</term><term>Iucz</term><term>Iucz gene</term><term>Late glycoprotein</term><term>Late stages</term><term>Marker genes</term><term>Mocarski</term><term>Negative regulation</term><term>Open circles</term><term>Opposite directions</term><term>Plasmid</term><term>Progeny genome</term><term>Promoter</term><term>Recombinant</term><term>Recombinant genomes</term><term>Recombinant virus</term><term>Recombinant viruses</term><term>Reversion rate</term><term>Simian virus</term><term>Viral</term><term>Viral proteins</term><term>Virion dnas</term><term>Virus replication</term><term>Virus vector</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: On the basis of a previous finding that the 7.8-kb HindIII-O fragment of the human cytomegalovirus strain Towne genome is nonessential for viral replication, we constructed a vector, pKM, that directs introduction of foreign genes by homologous recombination precisely replacing the O fragment. Using this vector, we constructed Towne-strain-derived recombinant viruses in which a chimeric lacZ gene fused to the simian virus 40 promoter and a poly(A) signal were inserted in place of the O fragment. Two types of recombinants were obtained which carried the chimeric gene in opposite directions. β-Galacto- sidase (βGal) was produced throughout the infection cycle in human embryonic lung cells infected with these recombinants, and the rate of its synthesis in the early stages of infection was comparable to that of synthesis of a 65-kDa viral glycoprotein, one of the abundantly produced viral proteins. The chimeric lacZ gene introduced was stable and no lacZ− revertants have been observed so far.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001D17 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 001D17 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:921795161D969F4C358BC51E4BF2CCDB6D35DFEA
|texte= Site-specific stable insertion into the human cytomegalovirus genome of a foreign gene under control of the SV40 promoter
}}
| This area was generated with Dilib version V0.6.33. |  |