The cytotoxicity of 22 sesquiterpenoid unsaturated dialdehydes, as determined by the neutral red absorption assay and by protein determination
Identifieur interne : 001156 ( Istex/Corpus ); précédent : 001155; suivant : 001157The cytotoxicity of 22 sesquiterpenoid unsaturated dialdehydes, as determined by the neutral red absorption assay and by protein determination
Auteurs : A. Forsby ; M. Andersson ; L. Lewan ; O. SternerSource :
- Toxicology in Vitro [ 0887-2333 ] ; 1991.
English descriptors
- Teeft :
- Absorption assay, Assay, Biological chemistry, Cell culture, Cell line, Chloroquine diphosphate, Comparative cytotoxicities, Control cells, Cytotoxic, Cytotoxic activity, Cytotoxicity, Cytotoxicity assays, Derivative, Dialdehydes, General cytotoxicity, Hamster fibroblasts, Ics0 values, Least mutagenic, Marasmic acid, Mercury chloride, Molecular targets, Monolayer culture, Mutagenic, Mutagenic activity, Natural products, Plate shaker, Polygodial, Potent cytotoxic agents, Protein content, Protein determination, Quantitative relationships, Sterner, Test substances, Total cell protein, Unsaturated, Unsaturated dialdehydes.
Abstract
Abstract: The neutral red absorption assay, complemented by protein determination, was used to measure the cytotoxic activity of 22 sesquiterpenoid unsaturated dialdehydes towards hamster fibroblasts in monolayer culture (cell line V79). No significant differences between the activities determined by the two assays were found for the compounds assayed, indicating that their inhibitory effects on cell growth are related to general cytotoxicity. In spite of the apparent similarity between the chemical structures of the assayed compounds, the most active was approximately 200 times more cytotoxic than the least active. Isovelleral and its derivatives are the most cytotoxic substances, but the ranking in order of general cytotoxicity within this subgroup differed from that found in a previous study of mutagenic activity. Marasmic acid and its derivatives are also potent cytotoxic agents, but two of the most cytotoxic compounds in this group have been reported to be the least mutagenic. Such differences between cytotoxic and mutagenic activities indicate that the molecular targets for these two activities differ. The cytotoxicity of polygodial found in this study is lower than that previously reported from other cytotoxicity assays, which suggests that different tests of general cytotoxicity may have different molecular targets. Because of the diverse cytotoxic activities of the unsaturated dialdehydes, it should be possible to study them in a quantitative structure-activity relationship (QSAR) multivariate analysis.
Url:
DOI: 10.1016/0887-2333(91)90043-D
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ISTEX:F1D35978D4A3D29F76E67BEAEA52D95342346588Le document en format XML
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Lewan</name><affiliation><mods:affiliation>Department of Zoophysiology, University of Lund, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Sterner, O" sort="Sterner, O" uniqKey="Sterner O" first="O." last="Sterner">O. Sterner</name><affiliation><mods:affiliation>Department of Organic Chemistry 2, University of Lund, Sweden</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:F1D35978D4A3D29F76E67BEAEA52D95342346588</idno><date when="1991" year="1991">1991</date><idno type="doi">10.1016/0887-2333(91)90043-D</idno><idno type="url">https://api.istex.fr/ark:/67375/6H6-6S1QFJ98-V/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">001156</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">001156</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">The cytotoxicity of 22 sesquiterpenoid unsaturated dialdehydes, as determined by the neutral red absorption assay and by protein determination</title><author><name sortKey="Forsby, A" sort="Forsby, A" uniqKey="Forsby A" first="A." last="Forsby">A. Forsby</name><affiliation><mods:affiliation>Department of Zoophysiology, University of Lund, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Andersson, M" sort="Andersson, M" uniqKey="Andersson M" first="M." last="Andersson">M. Andersson</name><affiliation><mods:affiliation>Department of Zoophysiology, University of Lund, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Lewan, L" sort="Lewan, L" uniqKey="Lewan L" first="L." last="Lewan">L. Lewan</name><affiliation><mods:affiliation>Department of Zoophysiology, University of Lund, Sweden</mods:affiliation></affiliation></author><author><name sortKey="Sterner, O" sort="Sterner, O" uniqKey="Sterner O" first="O." last="Sterner">O. Sterner</name><affiliation><mods:affiliation>Department of Organic Chemistry 2, University of Lund, Sweden</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Toxicology in Vitro</title><title level="j" type="abbrev">TIV</title><idno type="ISSN">0887-2333</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1991">1991</date><biblScope unit="volume">5</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="9">9</biblScope><biblScope unit="page" to="14">14</biblScope></imprint><idno type="ISSN">0887-2333</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0887-2333</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Absorption assay</term><term>Assay</term><term>Biological chemistry</term><term>Cell culture</term><term>Cell line</term><term>Chloroquine diphosphate</term><term>Comparative cytotoxicities</term><term>Control cells</term><term>Cytotoxic</term><term>Cytotoxic activity</term><term>Cytotoxicity</term><term>Cytotoxicity assays</term><term>Derivative</term><term>Dialdehydes</term><term>General cytotoxicity</term><term>Hamster fibroblasts</term><term>Ics0 values</term><term>Least mutagenic</term><term>Marasmic acid</term><term>Mercury chloride</term><term>Molecular targets</term><term>Monolayer culture</term><term>Mutagenic</term><term>Mutagenic activity</term><term>Natural products</term><term>Plate shaker</term><term>Polygodial</term><term>Potent cytotoxic agents</term><term>Protein content</term><term>Protein determination</term><term>Quantitative relationships</term><term>Sterner</term><term>Test substances</term><term>Total cell protein</term><term>Unsaturated</term><term>Unsaturated dialdehydes</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The neutral red absorption assay, complemented by protein determination, was used to measure the cytotoxic activity of 22 sesquiterpenoid unsaturated dialdehydes towards hamster fibroblasts in monolayer culture (cell line V79). No significant differences between the activities determined by the two assays were found for the compounds assayed, indicating that their inhibitory effects on cell growth are related to general cytotoxicity. In spite of the apparent similarity between the chemical structures of the assayed compounds, the most active was approximately 200 times more cytotoxic than the least active. Isovelleral and its derivatives are the most cytotoxic substances, but the ranking in order of general cytotoxicity within this subgroup differed from that found in a previous study of mutagenic activity. Marasmic acid and its derivatives are also potent cytotoxic agents, but two of the most cytotoxic compounds in this group have been reported to be the least mutagenic. Such differences between cytotoxic and mutagenic activities indicate that the molecular targets for these two activities differ. The cytotoxicity of polygodial found in this study is lower than that previously reported from other cytotoxicity assays, which suggests that different tests of general cytotoxicity may have different molecular targets. Because of the diverse cytotoxic activities of the unsaturated dialdehydes, it should be possible to study them in a quantitative structure-activity relationship (QSAR) multivariate analysis.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>dialdehydes</json:string><json:string>cytotoxicity</json:string><json:string>unsaturated dialdehydes</json:string><json:string>cytotoxic</json:string><json:string>assay</json:string><json:string>sterner</json:string><json:string>mutagenic</json:string><json:string>protein determination</json:string><json:string>polygodial</json:string><json:string>mercury chloride</json:string><json:string>ics0 values</json:string><json:string>mutagenic activity</json:string><json:string>unsaturated</json:string><json:string>general cytotoxicity</json:string><json:string>chloroquine diphosphate</json:string><json:string>derivative</json:string><json:string>cytotoxic activity</json:string><json:string>natural products</json:string><json:string>absorption assay</json:string><json:string>biological chemistry</json:string><json:string>cell line</json:string><json:string>marasmic acid</json:string><json:string>protein content</json:string><json:string>molecular targets</json:string><json:string>least mutagenic</json:string><json:string>monolayer culture</json:string><json:string>total cell protein</json:string><json:string>cell culture</json:string><json:string>control cells</json:string><json:string>potent cytotoxic agents</json:string><json:string>test substances</json:string><json:string>plate shaker</json:string><json:string>hamster fibroblasts</json:string><json:string>comparative cytotoxicities</json:string><json:string>quantitative relationships</json:string><json:string>cytotoxicity assays</json:string></teeft></keywords><author><json:item><name>A. Forsby</name><affiliations><json:string>Department of Zoophysiology, University of Lund, Sweden</json:string></affiliations></json:item><json:item><name>M. Andersson</name><affiliations><json:string>Department of Zoophysiology, University of Lund, Sweden</json:string></affiliations></json:item><json:item><name>L. Lewan</name><affiliations><json:string>Department of Zoophysiology, University of Lund, Sweden</json:string></affiliations></json:item><json:item><name>O. Sterner</name><affiliations><json:string>Department of Organic Chemistry 2, University of Lund, Sweden</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>EMEM : Eagle's minimal essential medium with Earle's salts</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>IC20/IC50 (NR) : the concentration that inhibits the neutral red absorption to 20/50%, respectively</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>IC20/IC50(PD) : the concentration that inhibits the protein content per well to 20/50%, respectively</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>NR : neutral red</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PBS : phosphate buffered saline</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>PD : protein determination</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>QSAR : quantitative structure-activity relationship</value></json:item></subject><arkIstex>ark:/67375/6H6-6S1QFJ98-V</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: The neutral red absorption assay, complemented by protein determination, was used to measure the cytotoxic activity of 22 sesquiterpenoid unsaturated dialdehydes towards hamster fibroblasts in monolayer culture (cell line V79). No significant differences between the activities determined by the two assays were found for the compounds assayed, indicating that their inhibitory effects on cell growth are related to general cytotoxicity. In spite of the apparent similarity between the chemical structures of the assayed compounds, the most active was approximately 200 times more cytotoxic than the least active. Isovelleral and its derivatives are the most cytotoxic substances, but the ranking in order of general cytotoxicity within this subgroup differed from that found in a previous study of mutagenic activity. Marasmic acid and its derivatives are also potent cytotoxic agents, but two of the most cytotoxic compounds in this group have been reported to be the least mutagenic. Such differences between cytotoxic and mutagenic activities indicate that the molecular targets for these two activities differ. The cytotoxicity of polygodial found in this study is lower than that previously reported from other cytotoxicity assays, which suggests that different tests of general cytotoxicity may have different molecular targets. Because of the diverse cytotoxic activities of the unsaturated dialdehydes, it should be possible to study them in a quantitative structure-activity relationship (QSAR) multivariate analysis.</abstract><qualityIndicators><score>7.124</score><pdfWordCount>2484</pdfWordCount><pdfCharCount>16926</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>6</pdfPageCount><pdfPageSize>504 x 756 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>220</abstractWordCount><abstractCharCount>1537</abstractCharCount><keywordCount>7</keywordCount></qualityIndicators><title>The cytotoxicity of 22 sesquiterpenoid unsaturated dialdehydes, as determined by the neutral red absorption assay and by protein determination</title><pii><json:string>0887-2333(91)90043-D</json:string></pii><genre><json:string>research-article</json:string></genre><host><title>Toxicology in Vitro</title><language><json:string>unknown</json:string></language><publicationDate>1991</publicationDate><issn><json:string>0887-2333</json:string></issn><pii><json:string>S0887-2333(00)X0092-6</json:string></pii><volume>5</volume><issue>1</issue><pages><first>9</first><last>14</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>7000</json:string><json:string>1991</json:string></date><geogName></geogName><orgName><json:string>FRG</json:string><json:string>STERNER Department of Organic Chemistry</json:string><json:string>University of Lund, Sweden</json:string><json:string>University of Stockholm</json:string><json:string>Department of Medical Research Cell Biology Team</json:string><json:string>Sigma Chemical Co.</json:string><json:string>University of Kaiserslautern</json:string><json:string>The Swedish Fund For Scientific Research Without Animal Experiments</json:string></orgName><orgName_funder><json:string>The Swedish Fund For Scientific Research Without Animal Experiments</json:string></orgName_funder><orgName_provider></orgName_provider><persName><json:string>H. 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(1951)</json:string><json:string>Nilsson et al., 1988</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-6S1QFJ98-V</json:string></ark><categories><wos><json:string>1 - science</json:string><json:string>2 - toxicology</json:string></wos><scienceMetrix><json:string>1 - health sciences</json:string><json:string>2 - biomedical research</json:string><json:string>3 - toxicology</json:string></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Pharmacology, Toxicology and Pharmaceutics</json:string><json:string>3 - Toxicology</json:string><json:string>1 - Health Sciences</json:string><json:string>2 - Medicine</json:string><json:string>3 - General Medicine</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences biologiques fondamentales et appliquees. psychologie</json:string></inist></categories><publicationDate>1991</publicationDate><copyrightDate>1991</copyrightDate><doi><json:string>10.1016/0887-2333(91)90043-D</json:string></doi><id>F1D35978D4A3D29F76E67BEAEA52D95342346588</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-6S1QFJ98-V/fulltext.pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-6S1QFJ98-V/bundle.zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/ark:/67375/6H6-6S1QFJ98-V/fulltext.tei"><teiHeader><fileDesc><titleStmt><title level="a">The cytotoxicity of 22 sesquiterpenoid unsaturated dialdehydes, as determined by the neutral red absorption assay and by protein determination</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">ELSEVIER</publisher><availability><licence><p>elsevier</p></licence></availability><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M"></p><date>1991</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note><note>Part 4 in a series on structure-activity relationships for unsaturated dialdehydes. For part 3, see Anke et al. (1989).</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">The cytotoxicity of 22 sesquiterpenoid unsaturated dialdehydes, as determined by the neutral red absorption assay and by protein determination</title><author xml:id="author-0000"><persName><forename type="first">A.</forename><surname>Forsby</surname></persName><note type="biography">Present address: Unit of Neurochemistry and Neurotoxicology, University of Stockholm, S-106 91, Stockholm, Sweden.</note><affiliation>Present address: Unit of Neurochemistry and Neurotoxicology, University of Stockholm, S-106 91, Stockholm, Sweden.</affiliation><affiliation>Department of Zoophysiology, University of Lund, Sweden</affiliation></author><author xml:id="author-0001"><persName><forename type="first">M.</forename><surname>Andersson</surname></persName><affiliation>Department of Zoophysiology, University of Lund, Sweden</affiliation></author><author xml:id="author-0002"><persName><forename type="first">L.</forename><surname>Lewan</surname></persName><affiliation>Department of Zoophysiology, University of Lund, Sweden</affiliation></author><author xml:id="author-0003"><persName><forename type="first">O.</forename><surname>Sterner</surname></persName><affiliation>Department of Organic Chemistry 2, University of Lund, Sweden</affiliation></author><idno type="istex">F1D35978D4A3D29F76E67BEAEA52D95342346588</idno><idno type="ark">ark:/67375/6H6-6S1QFJ98-V</idno><idno type="DOI">10.1016/0887-2333(91)90043-D</idno><idno type="PII">0887-2333(91)90043-D</idno></analytic><monogr><title level="j">Toxicology in Vitro</title><title level="j" type="abbrev">TIV</title><idno type="pISSN">0887-2333</idno><idno type="PII">S0887-2333(00)X0092-6</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1991"></date><biblScope unit="volume">5</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="9">9</biblScope><biblScope unit="page" to="14">14</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1991</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Abstract: The neutral red absorption assay, complemented by protein determination, was used to measure the cytotoxic activity of 22 sesquiterpenoid unsaturated dialdehydes towards hamster fibroblasts in monolayer culture (cell line V79). No significant differences between the activities determined by the two assays were found for the compounds assayed, indicating that their inhibitory effects on cell growth are related to general cytotoxicity. In spite of the apparent similarity between the chemical structures of the assayed compounds, the most active was approximately 200 times more cytotoxic than the least active. Isovelleral and its derivatives are the most cytotoxic substances, but the ranking in order of general cytotoxicity within this subgroup differed from that found in a previous study of mutagenic activity. Marasmic acid and its derivatives are also potent cytotoxic agents, but two of the most cytotoxic compounds in this group have been reported to be the least mutagenic. Such differences between cytotoxic and mutagenic activities indicate that the molecular targets for these two activities differ. The cytotoxicity of polygodial found in this study is lower than that previously reported from other cytotoxicity assays, which suggests that different tests of general cytotoxicity may have different molecular targets. Because of the diverse cytotoxic activities of the unsaturated dialdehydes, it should be possible to study them in a quantitative structure-activity relationship (QSAR) multivariate analysis.</p></abstract><textClass><keywords scheme="keyword"><list><head>Abbreviations</head><item><term>EMEM</term><term>Eagle's minimal essential medium with Earle's salts</term></item><item><term>IC20/IC50 (NR)</term><term>the concentration that inhibits the neutral red absorption to 20/50%, respectively</term></item><item><term>IC20/IC50(PD)</term><term>the concentration that inhibits the protein content per well to 20/50%, respectively</term></item><item><term>NR</term><term>neutral red</term></item><item><term>PBS</term><term>phosphate buffered saline</term></item><item><term>PD</term><term>protein determination</term></item><item><term>QSAR</term><term>quantitative structure-activity relationship</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="1990-07-30">Modified</change><change when="1991">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-6S1QFJ98-V/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>TIV</jid><aid>9190043D</aid><ce:pii>0887-2333(91)90043-D</ce:pii><ce:doi>10.1016/0887-2333(91)90043-D</ce:doi><ce:copyright type="unknown" year="1991"></ce:copyright></item-info><head><ce:article-footnote><ce:label>☆</ce:label><ce:note-para>Part 4 in a series on structure-activity relationships for unsaturated dialdehydes. For part 3, see Anke <ce:italic>et al.</ce:italic> (1989).</ce:note-para></ce:article-footnote><ce:title>The cytotoxicity of 22 sesquiterpenoid unsaturated dialdehydes, as determined by the neutral red absorption assay and by protein determination</ce:title><ce:author-group><ce:author><ce:given-name>A.</ce:given-name><ce:surname>Forsby</ce:surname><ce:cross-ref refid="FN1"><ce:sup>†</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>M.</ce:given-name><ce:surname>Andersson</ce:surname></ce:author><ce:author><ce:given-name>L.</ce:given-name><ce:surname>Lewan</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Zoophysiology, University of Lund, Sweden</ce:textfn></ce:affiliation><ce:footnote id="FN1"><ce:label>†</ce:label><ce:note-para>Present address: Unit of Neurochemistry and Neurotoxicology, University of Stockholm, S-106 91, Stockholm, Sweden.</ce:note-para></ce:footnote></ce:author-group><ce:author-group><ce:author><ce:given-name>O.</ce:given-name><ce:surname>Sterner</ce:surname></ce:author><ce:affiliation><ce:textfn>Department of Organic Chemistry 2, University of Lund, Sweden</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="23" month="4" year="1990"></ce:date-received><ce:date-revised day="30" month="7" year="1990"></ce:date-revised><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The neutral red absorption assay, complemented by protein determination, was used to measure the cytotoxic activity of 22 sesquiterpenoid unsaturated dialdehydes towards hamster fibroblasts in monolayer culture (cell line V79). No significant differences between the activities determined by the two assays were found for the compounds assayed, indicating that their inhibitory effects on cell growth are related to general cytotoxicity. In spite of the apparent similarity between the chemical structures of the assayed compounds, the most active was approximately 200 times more cytotoxic than the least active. Isovelleral and its derivatives are the most cytotoxic substances, but the ranking in order of general cytotoxicity within this subgroup differed from that found in a previous study of mutagenic activity. Marasmic acid and its derivatives are also potent cytotoxic agents, but two of the most cytotoxic compounds in this group have been reported to be the least mutagenic. Such differences between cytotoxic and mutagenic activities indicate that the molecular targets for these two activities differ. The cytotoxicity of polygodial found in this study is lower than that previously reported from other cytotoxicity assays, which suggests that different tests of general cytotoxicity may have different molecular targets. Because of the diverse cytotoxic activities of the unsaturated dialdehydes, it should be possible to study them in a quantitative structure-activity relationship (QSAR) multivariate analysis.</ce:simple-para></ce:abstract-sec></ce:abstract><ce:keywords class="abr"><ce:section-title>Abbreviations</ce:section-title><ce:keyword><ce:text>EMEM</ce:text><ce:keyword><ce:text>Eagle's minimal essential medium with Earle's salts</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>IC<ce:inf>20</ce:inf>/IC<ce:inf>50</ce:inf> (NR)</ce:text><ce:keyword><ce:text>the concentration that inhibits the neutral red absorption to 20/50%, respectively</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>IC<ce:inf>20</ce:inf>/IC<ce:inf>50</ce:inf>(PD)</ce:text><ce:keyword><ce:text>the concentration that inhibits the protein content per well to 20/50%, respectively</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>NR</ce:text><ce:keyword><ce:text>neutral red</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>PBS</ce:text><ce:keyword><ce:text>phosphate buffered saline</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>PD</ce:text><ce:keyword><ce:text>protein determination</ce:text></ce:keyword></ce:keyword><ce:keyword><ce:text>QSAR</ce:text><ce:keyword><ce:text>quantitative structure-activity relationship</ce:text></ce:keyword></ce:keyword></ce:keywords></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>The cytotoxicity of 22 sesquiterpenoid unsaturated dialdehydes, as determined by the neutral red absorption assay and by protein determination</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>The cytotoxicity of 22 sesquiterpenoid unsaturated dialdehydes, as determined by the neutral red absorption assay and by protein determination</title></titleInfo><name type="personal"><namePart type="given">A.</namePart><namePart type="family">Forsby</namePart><affiliation>Department of Zoophysiology, University of Lund, Sweden</affiliation><description>Present address: Unit of Neurochemistry and Neurotoxicology, University of Stockholm, S-106 91, Stockholm, Sweden.</description><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">M.</namePart><namePart type="family">Andersson</namePart><affiliation>Department of Zoophysiology, University of Lund, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">L.</namePart><namePart type="family">Lewan</namePart><affiliation>Department of Zoophysiology, University of Lund, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">O.</namePart><namePart type="family">Sterner</namePart><affiliation>Department of Organic Chemistry 2, University of Lund, Sweden</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1991</dateIssued><dateModified encoding="w3cdtf">1990-07-30</dateModified><copyrightDate encoding="w3cdtf">1991</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The neutral red absorption assay, complemented by protein determination, was used to measure the cytotoxic activity of 22 sesquiterpenoid unsaturated dialdehydes towards hamster fibroblasts in monolayer culture (cell line V79). No significant differences between the activities determined by the two assays were found for the compounds assayed, indicating that their inhibitory effects on cell growth are related to general cytotoxicity. In spite of the apparent similarity between the chemical structures of the assayed compounds, the most active was approximately 200 times more cytotoxic than the least active. Isovelleral and its derivatives are the most cytotoxic substances, but the ranking in order of general cytotoxicity within this subgroup differed from that found in a previous study of mutagenic activity. Marasmic acid and its derivatives are also potent cytotoxic agents, but two of the most cytotoxic compounds in this group have been reported to be the least mutagenic. Such differences between cytotoxic and mutagenic activities indicate that the molecular targets for these two activities differ. The cytotoxicity of polygodial found in this study is lower than that previously reported from other cytotoxicity assays, which suggests that different tests of general cytotoxicity may have different molecular targets. Because of the diverse cytotoxic activities of the unsaturated dialdehydes, it should be possible to study them in a quantitative structure-activity relationship (QSAR) multivariate analysis.</abstract><note>Part 4 in a series on structure-activity relationships for unsaturated dialdehydes. For part 3, see Anke et al. (1989).</note><subject><genre>Abbreviations</genre><topic>EMEM : Eagle's minimal essential medium with Earle's salts</topic><topic>IC20/IC50 (NR) : the concentration that inhibits the neutral red absorption to 20/50%, respectively</topic><topic>IC20/IC50(PD) : the concentration that inhibits the protein content per well to 20/50%, respectively</topic><topic>NR : neutral red</topic><topic>PBS : phosphate buffered saline</topic><topic>PD : protein determination</topic><topic>QSAR : quantitative structure-activity relationship</topic></subject><relatedItem type="host"><titleInfo><title>Toxicology in Vitro</title></titleInfo><titleInfo type="abbreviated"><title>TIV</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1991</dateIssued></originInfo><identifier type="ISSN">0887-2333</identifier><identifier type="PII">S0887-2333(00)X0092-6</identifier><part><date>1991</date><detail type="volume"><number>5</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>101</end></extent><extent unit="pages"><start>9</start><end>14</end></extent></part></relatedItem><identifier type="istex">F1D35978D4A3D29F76E67BEAEA52D95342346588</identifier><identifier type="ark">ark:/67375/6H6-6S1QFJ98-V</identifier><identifier type="DOI">10.1016/0887-2333(91)90043-D</identifier><identifier type="PII">0887-2333(91)90043-D</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/6H6-6S1QFJ98-V/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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