Chloroquine relieves acute lung injury in rats with acute hemorrhagic necrotizing pancreatitis
Identifieur interne : 000289 ( Istex/Checkpoint ); précédent : 000288; suivant : 000290Chloroquine relieves acute lung injury in rats with acute hemorrhagic necrotizing pancreatitis
Auteurs : Lei Zhang [République populaire de Chine] ; Yan Chen [République populaire de Chine] ; Lin Wang [République populaire de Chine] ; Xiao-Ping Chen [République populaire de Chine] ; Wan-Guang Zhang [République populaire de Chine] ; Chun-You Wang [République populaire de Chine] ; He-Shui Wu [République populaire de Chine]Source :
- Journal of Huazhong University of Science and Technology [Medical Sciences] [ 1672-0733 ] ; 2013-06-01.
English descriptors
- KwdEn :
Abstract
Summary: This study preliminarily investigated the mechanism by which chloroquine (CQ) relieves acute lung injury (ALI) complicated in acute hemorrhagic necrotizing pancreatitis (AHNP). Sixty male Wistar rats were randomized into sham-operated group (group A, n=10), AHNP group (group B, n=10), L-arginine-treated group (group C, n=10), L-N-nitro-L-arginine methyl ester (NAME)-treated group (group D, n=10), CQ-treated group (group E, n=10) and CQ+L-NAME-treated group (group F, n=10). TLR4 expression was measured by using real time-PCR and Western blotting respectively. The results showed that, in the group B, the expression of TLR4 and the levels of TNF-α and IL-6 in the lungs were significantly increased, and the nitric oxide (NO) concentration was reduced, as compared with those in the group A (P<0.05 or P<0.01). Lung injury was aggravated with the increased expression of TLR4. When the inhibitor and stimulator of TLR4, namely L-Arg and L-NAME, were added respectively, lung injury was correspondingly relieved or aggravated (P<0.05 or P<0.01). In the group E, TLR4 expression was substantially lower and NO concentration higher than those in the group B (P<0.05 or P<0.01). However, in the group F, NO concentration was markedly decreased, and the inhibitory effect of CQ on TLR4 expression and the relief of lung injury were weakened when compared with those in the group E (P<0.05 or P<0.01). It was concluded that TLR4 may play an important role in the pathogenesis and development of ALI complicated in AHNP. CQ could relieve ALI by decreasing the TLR4 expression and increasing the NO release.
Url:
DOI: 10.1007/s11596-013-1124-9
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
ISTEX:7BBD09EA85CFCCFC554668924D475360C3E84F29Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Chloroquine relieves acute lung injury in rats with acute hemorrhagic necrotizing pancreatitis</title><author><name sortKey="Zhang, Lei" sort="Zhang, Lei" uniqKey="Zhang L" first="Lei" last="Zhang">Lei Zhang</name></author><author><name sortKey="Chen, Yan" sort="Chen, Yan" uniqKey="Chen Y" first="Yan" last="Chen">Yan Chen</name></author><author><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name></author><author><name sortKey="Chen, Xiao Ping" sort="Chen, Xiao Ping" uniqKey="Chen X" first="Xiao-Ping" last="Chen">Xiao-Ping Chen</name></author><author><name sortKey="Zhang, Wan Guang" sort="Zhang, Wan Guang" uniqKey="Zhang W" first="Wan-Guang" last="Zhang">Wan-Guang Zhang</name></author><author><name sortKey="Wang, Chun You" sort="Wang, Chun You" uniqKey="Wang C" first="Chun-You" last="Wang">Chun-You Wang</name></author><author><name sortKey="Wu, He Shui" sort="Wu, He Shui" uniqKey="Wu H" first="He-Shui" last="Wu">He-Shui Wu</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:7BBD09EA85CFCCFC554668924D475360C3E84F29</idno><date when="2013" year="2013">2013</date><idno type="doi">10.1007/s11596-013-1124-9</idno><idno type="url">https://api.istex.fr/ark:/67375/VQC-9GMKW13S-L/fulltext.pdf</idno><idno type="wicri:Area/Istex/Corpus">000621</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000621</idno><idno type="wicri:Area/Istex/Curation">000621</idno><idno type="wicri:Area/Istex/Checkpoint">000289</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000289</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Chloroquine relieves acute lung injury in rats with acute hemorrhagic necrotizing pancreatitis</title><author><name sortKey="Zhang, Lei" sort="Zhang, Lei" uniqKey="Zhang L" first="Lei" last="Zhang">Lei Zhang</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">République populaire de Chine</country></affiliation></author><author><name sortKey="Chen, Yan" sort="Chen, Yan" uniqKey="Chen Y" first="Yan" last="Chen">Yan Chen</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pediatrics, Huazhong University of Science and Technology, 430022, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Pediatrics, Huazhong University of Science and Technology, 430022, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Chen, Xiao Ping" sort="Chen, Xiao Ping" uniqKey="Chen X" first="Xiao-Ping" last="Chen">Xiao-Ping Chen</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">République populaire de Chine</country></affiliation></author><author><name sortKey="Zhang, Wan Guang" sort="Zhang, Wan Guang" uniqKey="Zhang W" first="Wan-Guang" last="Zhang">Wan-Guang Zhang</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wang, Chun You" sort="Wang, Chun You" uniqKey="Wang C" first="Chun-You" last="Wang">Chun-You Wang</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation></author><author><name sortKey="Wu, He Shui" sort="Wu, He Shui" uniqKey="Wu H" first="He-Shui" last="Wu">He-Shui Wu</name><affiliation wicri:level="3"><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName></affiliation><affiliation wicri:level="1"><country wicri:rule="url">République populaire de Chine</country></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Huazhong University of Science and Technology [Medical Sciences]</title><title level="j" type="sub">Medical Sciences</title><title level="j" type="abbrev">J. Huazhong Univ. Sci. Technol. [Med. Sci.]</title><idno type="ISSN">1672-0733</idno><idno type="eISSN">1993-1352</idno><imprint><publisher>Huazhong University of Science and Technology</publisher><pubPlace>Heidelberg</pubPlace><date type="published" when="2013-06-01">2013-06-01</date><biblScope unit="volume">33</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="357">357</biblScope><biblScope unit="page" to="360">360</biblScope></imprint><idno type="ISSN">1672-0733</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1672-0733</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>L -arginine</term><term>N-nitro- L -arginine methyl ester</term><term>Toll-like receptor</term><term>acute hemorrhagic necrotizing pancreatitis</term><term>chloroquine</term><term>lung</term><term>nitric oxide</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: This study preliminarily investigated the mechanism by which chloroquine (CQ) relieves acute lung injury (ALI) complicated in acute hemorrhagic necrotizing pancreatitis (AHNP). Sixty male Wistar rats were randomized into sham-operated group (group A, n=10), AHNP group (group B, n=10), L-arginine-treated group (group C, n=10), L-N-nitro-L-arginine methyl ester (NAME)-treated group (group D, n=10), CQ-treated group (group E, n=10) and CQ+L-NAME-treated group (group F, n=10). TLR4 expression was measured by using real time-PCR and Western blotting respectively. The results showed that, in the group B, the expression of TLR4 and the levels of TNF-α and IL-6 in the lungs were significantly increased, and the nitric oxide (NO) concentration was reduced, as compared with those in the group A (P<0.05 or P<0.01). Lung injury was aggravated with the increased expression of TLR4. When the inhibitor and stimulator of TLR4, namely L-Arg and L-NAME, were added respectively, lung injury was correspondingly relieved or aggravated (P<0.05 or P<0.01). In the group E, TLR4 expression was substantially lower and NO concentration higher than those in the group B (P<0.05 or P<0.01). However, in the group F, NO concentration was markedly decreased, and the inhibitory effect of CQ on TLR4 expression and the relief of lung injury were weakened when compared with those in the group E (P<0.05 or P<0.01). It was concluded that TLR4 may play an important role in the pathogenesis and development of ALI complicated in AHNP. CQ could relieve ALI by decreasing the TLR4 expression and increasing the NO release.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Hubei</li></region><settlement><li>Wuhan</li></settlement></list><tree><country name="République populaire de Chine"><region name="Hubei"><name sortKey="Zhang, Lei" sort="Zhang, Lei" uniqKey="Zhang L" first="Lei" last="Zhang">Lei Zhang</name></region><name sortKey="Chen, Xiao Ping" sort="Chen, Xiao Ping" uniqKey="Chen X" first="Xiao-Ping" last="Chen">Xiao-Ping Chen</name><name sortKey="Chen, Xiao Ping" sort="Chen, Xiao Ping" uniqKey="Chen X" first="Xiao-Ping" last="Chen">Xiao-Ping Chen</name><name sortKey="Chen, Yan" sort="Chen, Yan" uniqKey="Chen Y" first="Yan" last="Chen">Yan Chen</name><name sortKey="Wang, Chun You" sort="Wang, Chun You" uniqKey="Wang C" first="Chun-You" last="Wang">Chun-You Wang</name><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name><name sortKey="Wu, He Shui" sort="Wu, He Shui" uniqKey="Wu H" first="He-Shui" last="Wu">He-Shui Wu</name><name sortKey="Wu, He Shui" sort="Wu, He Shui" uniqKey="Wu H" first="He-Shui" last="Wu">He-Shui Wu</name><name sortKey="Zhang, Lei" sort="Zhang, Lei" uniqKey="Zhang L" first="Lei" last="Zhang">Lei Zhang</name><name sortKey="Zhang, Wan Guang" sort="Zhang, Wan Guang" uniqKey="Zhang W" first="Wan-Guang" last="Zhang">Wan-Guang Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/ChloroquineV1/Data/Istex/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000289 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Checkpoint/biblio.hfd -nk 000289 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= ChloroquineV1
|flux= Istex
|étape= Checkpoint
|type= RBID
|clé= ISTEX:7BBD09EA85CFCCFC554668924D475360C3E84F29
|texte= Chloroquine relieves acute lung injury in rats with acute hemorrhagic necrotizing pancreatitis
}}
| This area was generated with Dilib version V0.6.33. |  |