Chloroquine relieves acute lung injury in rats with acute hemorrhagic necrotizing pancreatitis
Identifieur interne : 000621 ( Istex/Corpus ); précédent : 000620; suivant : 000622Chloroquine relieves acute lung injury in rats with acute hemorrhagic necrotizing pancreatitis
Auteurs : Lei Zhang ; Yan Chen ; Lin Wang ; Xiao-Ping Chen ; Wan-Guang Zhang ; Chun-You Wang ; He-Shui WuSource :
- Journal of Huazhong University of Science and Technology [Medical Sciences] [ 1672-0733 ] ; 2013-06-01.
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Abstract
Summary: This study preliminarily investigated the mechanism by which chloroquine (CQ) relieves acute lung injury (ALI) complicated in acute hemorrhagic necrotizing pancreatitis (AHNP). Sixty male Wistar rats were randomized into sham-operated group (group A, n=10), AHNP group (group B, n=10), L-arginine-treated group (group C, n=10), L-N-nitro-L-arginine methyl ester (NAME)-treated group (group D, n=10), CQ-treated group (group E, n=10) and CQ+L-NAME-treated group (group F, n=10). TLR4 expression was measured by using real time-PCR and Western blotting respectively. The results showed that, in the group B, the expression of TLR4 and the levels of TNF-α and IL-6 in the lungs were significantly increased, and the nitric oxide (NO) concentration was reduced, as compared with those in the group A (P<0.05 or P<0.01). Lung injury was aggravated with the increased expression of TLR4. When the inhibitor and stimulator of TLR4, namely L-Arg and L-NAME, were added respectively, lung injury was correspondingly relieved or aggravated (P<0.05 or P<0.01). In the group E, TLR4 expression was substantially lower and NO concentration higher than those in the group B (P<0.05 or P<0.01). However, in the group F, NO concentration was markedly decreased, and the inhibitory effect of CQ on TLR4 expression and the relief of lung injury were weakened when compared with those in the group E (P<0.05 or P<0.01). It was concluded that TLR4 may play an important role in the pathogenesis and development of ALI complicated in AHNP. CQ could relieve ALI by decreasing the TLR4 expression and increasing the NO release.
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DOI: 10.1007/s11596-013-1124-9
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Chun You" uniqKey="Wang C" first="Chun-You" last="Wang">Chun-You Wang</name><affiliation><mods:affiliation>Center of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China</mods:affiliation></affiliation></author><author><name sortKey="Wu, He Shui" sort="Wu, He Shui" uniqKey="Wu H" first="He-Shui" last="Wu">He-Shui Wu</name><affiliation><mods:affiliation>Center of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: whs1898@public.wh.hb.cn</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:7BBD09EA85CFCCFC554668924D475360C3E84F29</idno><date when="2013" year="2013">2013</date><idno type="doi">10.1007/s11596-013-1124-9</idno><idno 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Pediatrics, Huazhong University of Science and Technology, 430022, Wuhan, China</mods:affiliation></affiliation></author><author><name sortKey="Wang, Lin" sort="Wang, Lin" uniqKey="Wang L" first="Lin" last="Wang">Lin Wang</name><affiliation><mods:affiliation>Department of Pediatrics, Huazhong University of Science and Technology, 430022, Wuhan, China</mods:affiliation></affiliation></author><author><name sortKey="Chen, Xiao Ping" sort="Chen, Xiao Ping" uniqKey="Chen X" first="Xiao-Ping" last="Chen">Xiao-Ping Chen</name><affiliation><mods:affiliation>Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: chenxp@medmail.com.cn</mods:affiliation></affiliation></author><author><name sortKey="Zhang, Wan Guang" sort="Zhang, Wan Guang" uniqKey="Zhang W" first="Wan-Guang" last="Zhang">Wan-Guang Zhang</name><affiliation><mods:affiliation>Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China</mods:affiliation></affiliation></author><author><name sortKey="Wang, Chun You" sort="Wang, Chun You" uniqKey="Wang C" first="Chun-You" last="Wang">Chun-You Wang</name><affiliation><mods:affiliation>Center of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China</mods:affiliation></affiliation></author><author><name sortKey="Wu, He Shui" sort="Wu, He Shui" uniqKey="Wu H" first="He-Shui" last="Wu">He-Shui Wu</name><affiliation><mods:affiliation>Center of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China</mods:affiliation></affiliation><affiliation><mods:affiliation>E-mail: whs1898@public.wh.hb.cn</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Huazhong University of Science and Technology [Medical Sciences]</title><title level="j" type="sub">Medical Sciences</title><title level="j" type="abbrev">J. Huazhong Univ. Sci. Technol. [Med. Sci.]</title><idno type="ISSN">1672-0733</idno><idno type="eISSN">1993-1352</idno><imprint><publisher>Huazhong University of Science and Technology</publisher><pubPlace>Heidelberg</pubPlace><date type="published" when="2013-06-01">2013-06-01</date><biblScope unit="volume">33</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="357">357</biblScope><biblScope unit="page" to="360">360</biblScope></imprint><idno type="ISSN">1672-0733</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">1672-0733</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>L -arginine</term><term>N-nitro- L -arginine methyl ester</term><term>Toll-like receptor</term><term>acute hemorrhagic necrotizing pancreatitis</term><term>chloroquine</term><term>lung</term><term>nitric oxide</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Summary: This study preliminarily investigated the mechanism by which chloroquine (CQ) relieves acute lung injury (ALI) complicated in acute hemorrhagic necrotizing pancreatitis (AHNP). Sixty male Wistar rats were randomized into sham-operated group (group A, n=10), AHNP group (group B, n=10), L-arginine-treated group (group C, n=10), L-N-nitro-L-arginine methyl ester (NAME)-treated group (group D, n=10), CQ-treated group (group E, n=10) and CQ+L-NAME-treated group (group F, n=10). TLR4 expression was measured by using real time-PCR and Western blotting respectively. The results showed that, in the group B, the expression of TLR4 and the levels of TNF-α and IL-6 in the lungs were significantly increased, and the nitric oxide (NO) concentration was reduced, as compared with those in the group A (P<0.05 or P<0.01). Lung injury was aggravated with the increased expression of TLR4. When the inhibitor and stimulator of TLR4, namely L-Arg and L-NAME, were added respectively, lung injury was correspondingly relieved or aggravated (P<0.05 or P<0.01). In the group E, TLR4 expression was substantially lower and NO concentration higher than those in the group B (P<0.05 or P<0.01). However, in the group F, NO concentration was markedly decreased, and the inhibitory effect of CQ on TLR4 expression and the relief of lung injury were weakened when compared with those in the group E (P<0.05 or P<0.01). It was concluded that TLR4 may play an important role in the pathogenesis and development of ALI complicated in AHNP. CQ could relieve ALI by decreasing the TLR4 expression and increasing the NO release.</div></front></TEI><istex><corpusName>springer-journals</corpusName><author><json:item><name>Lei Zhang</name><affiliations><json:string>Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China</json:string><json:string>E-mail: zhangl@tjh.tjmu.edu.cn</json:string></affiliations></json:item><json:item><name>Yan Chen</name><affiliations><json:string>Department of Pediatrics, Huazhong University of Science and Technology, 430022, Wuhan, China</json:string></affiliations></json:item><json:item><name>Lin Wang</name><affiliations><json:string>Department of Pediatrics, Huazhong University of Science and Technology, 430022, Wuhan, China</json:string></affiliations></json:item><json:item><name>Xiao-ping Chen</name><affiliations><json:string>Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China</json:string><json:string>E-mail: chenxp@medmail.com.cn</json:string></affiliations></json:item><json:item><name>Wan-guang Zhang</name><affiliations><json:string>Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China</json:string></affiliations></json:item><json:item><name>Chun-you Wang</name><affiliations><json:string>Center of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China</json:string></affiliations></json:item><json:item><name>He-shui Wu</name><affiliations><json:string>Center of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China</json:string><json:string>E-mail: whs1898@public.wh.hb.cn</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>Toll-like receptor</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>acute hemorrhagic necrotizing pancreatitis</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>lung</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>chloroquine</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>nitric oxide</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>L -arginine</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>N-nitro- L -arginine methyl ester</value></json:item></subject><articleId><json:string>1124</json:string><json:string>s11596-013-1124-9</json:string></articleId><arkIstex>ark:/67375/VQC-9GMKW13S-L</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>OriginalPaper</json:string></originalGenre><abstract>Summary: This study preliminarily investigated the mechanism by which chloroquine (CQ) relieves acute lung injury (ALI) complicated in acute hemorrhagic necrotizing pancreatitis (AHNP). Sixty male Wistar rats were randomized into sham-operated group (group A, n=10), AHNP group (group B, n=10), L-arginine-treated group (group C, n=10), L-N-nitro-L-arginine methyl ester (NAME)-treated group (group D, n=10), CQ-treated group (group E, n=10) and CQ+L-NAME-treated group (group F, n=10). TLR4 expression was measured by using real time-PCR and Western blotting respectively. The results showed that, in the group B, the expression of TLR4 and the levels of TNF-α and IL-6 in the lungs were significantly increased, and the nitric oxide (NO) concentration was reduced, as compared with those in the group A (P>0.05 or P>0.01). Lung injury was aggravated with the increased expression of TLR4. When the inhibitor and stimulator of TLR4, namely L-Arg and L-NAME, were added respectively, lung injury was correspondingly relieved or aggravated (P>0.05 or P>0.01). In the group E, TLR4 expression was substantially lower and NO concentration higher than those in the group B (P>0.05 or P>0.01). However, in the group F, NO concentration was markedly decreased, and the inhibitory effect of CQ on TLR4 expression and the relief of lung injury were weakened when compared with those in the group E (P>0.05 or P>0.01). It was concluded that TLR4 may play an important role in the pathogenesis and development of ALI complicated in AHNP. CQ could relieve ALI by decreasing the TLR4 expression and increasing the NO release.</abstract><qualityIndicators><score>7.836</score><pdfWordCount>2860</pdfWordCount><pdfCharCount>18533</pdfCharCount><pdfVersion>1.4</pdfVersion><pdfPageCount>4</pdfPageCount><pdfPageSize>595.22 x 842 pts (A4)</pdfPageSize><refBibsNative>false</refBibsNative><abstractWordCount>248</abstractWordCount><abstractCharCount>1613</abstractCharCount><keywordCount>7</keywordCount></qualityIndicators><title>Chloroquine relieves acute lung injury in rats with acute hemorrhagic necrotizing pancreatitis</title><genre><json:string>research-article</json:string></genre><host><title>Journal of Huazhong University of Science and Technology [Medical 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pancreatitis</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher scheme="https://scientific-publisher.data.istex.fr">Huazhong University of Science and Technology</publisher><pubPlace>Heidelberg</pubPlace><availability><licence><p>Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg, 2013</p></licence><p scheme="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</p></availability><date>2012-12-14</date></publicationStmt><notesStmt><note type="research-article" scheme="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</note><note type="journal" scheme="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Chloroquine relieves acute lung injury in rats with acute hemorrhagic necrotizing pancreatitis</title><author xml:id="author-0000"><persName><forename type="first">Lei</forename><surname>Zhang</surname><surname type="transliteration">张 磊</surname></persName><email>zhangl@tjh.tjmu.edu.cn</email><affiliation>Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China</affiliation></author><author xml:id="author-0001"><persName><forename type="first">Yan</forename><surname>Chen</surname><surname type="transliteration">陈 燕</surname></persName><affiliation>Department of Pediatrics, Huazhong University of Science and Technology, 430022, Wuhan, China</affiliation></author><author xml:id="author-0002"><persName><forename type="first">Lin</forename><surname>Wang</surname><surname type="transliteration">王 琳</surname></persName><affiliation>Department of Pediatrics, Huazhong University of Science and Technology, 430022, Wuhan, China</affiliation></author><author xml:id="author-0003" corresp="yes"><persName><forename type="first">Xiao-ping</forename><surname>Chen</surname><surname type="transliteration">陈孝平</surname></persName><email>chenxp@medmail.com.cn</email><affiliation>Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China</affiliation></author><author xml:id="author-0004"><persName><forename type="first">Wan-guang</forename><surname>Zhang</surname><surname type="transliteration">张万广</surname></persName><affiliation>Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China</affiliation></author><author xml:id="author-0005"><persName><forename type="first">Chun-you</forename><surname>Wang</surname><surname type="transliteration">王春友</surname></persName><affiliation>Center of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China</affiliation></author><author xml:id="author-0006" corresp="yes"><persName><forename type="first">He-shui</forename><surname>Wu</surname><surname type="transliteration">吴河水</surname></persName><email>whs1898@public.wh.hb.cn</email><affiliation>Center of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China</affiliation></author><idno type="istex">7BBD09EA85CFCCFC554668924D475360C3E84F29</idno><idno type="ark">ark:/67375/VQC-9GMKW13S-L</idno><idno type="DOI">10.1007/s11596-013-1124-9</idno><idno type="article-id">1124</idno><idno type="article-id">s11596-013-1124-9</idno></analytic><monogr><title level="j">Journal of Huazhong University of Science and Technology [Medical Sciences]</title><title level="j" type="sub">Medical Sciences</title><title level="j" type="abbrev">J. Huazhong Univ. Sci. Technol. [Med. Sci.]</title><idno type="pISSN">1672-0733</idno><idno type="eISSN">1993-1352</idno><idno type="journal-ID">true</idno><idno type="issue-article-count">25</idno><idno type="volume-issue-count">6</idno><imprint><publisher>Huazhong University of Science and Technology</publisher><pubPlace>Heidelberg</pubPlace><date type="published" when="2013-06-01"></date><biblScope unit="volume">33</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="357">357</biblScope><biblScope unit="page" to="360">360</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>2012-12-14</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>Summary: This study preliminarily investigated the mechanism by which chloroquine (CQ) relieves acute lung injury (ALI) complicated in acute hemorrhagic necrotizing pancreatitis (AHNP). Sixty male Wistar rats were randomized into sham-operated group (group A, n=10), AHNP group (group B, n=10), L-arginine-treated group (group C, n=10), L-N-nitro-L-arginine methyl ester (NAME)-treated group (group D, n=10), CQ-treated group (group E, n=10) and CQ+L-NAME-treated group (group F, n=10). TLR4 expression was measured by using real time-PCR and Western blotting respectively. The results showed that, in the group B, the expression of TLR4 and the levels of TNF-α and IL-6 in the lungs were significantly increased, and the nitric oxide (NO) concentration was reduced, as compared with those in the group A (P<0.05 or P<0.01). Lung injury was aggravated with the increased expression of TLR4. When the inhibitor and stimulator of TLR4, namely L-Arg and L-NAME, were added respectively, lung injury was correspondingly relieved or aggravated (P<0.05 or P<0.01). In the group E, TLR4 expression was substantially lower and NO concentration higher than those in the group B (P<0.05 or P<0.01). However, in the group F, NO concentration was markedly decreased, and the inhibitory effect of CQ on TLR4 expression and the relief of lung injury were weakened when compared with those in the group E (P<0.05 or P<0.01). It was concluded that TLR4 may play an important role in the pathogenesis and development of ALI complicated in AHNP. CQ could relieve ALI by decreasing the TLR4 expression and increasing the NO release.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Key words</head><item><term>Toll-like receptor</term></item><item><term>acute hemorrhagic necrotizing pancreatitis</term></item><item><term>lung</term></item><item><term>chloroquine</term></item><item><term>nitric oxide</term></item><item><term>L -arginine</term></item><item><term>N-nitro- L -arginine methyl ester</term></item></list></keywords></textClass><textClass><keywords scheme="Journal-Subject-Collection"><list><label>SC11</label><item><term>Medicine</term></item></list></keywords></textClass><textClass><keywords scheme="Journal-Subject-Group"><list><label>SCH</label><item><term>Medicine & Public Health</term></item><label>SCH00007</label><item><term>Medicine/Public Health, general</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2012-12-14">Created</change><change when="2013-06-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-9GMKW13S-L/fulltext.txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus springer-journals not found" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//Springer-Verlag//DTD A++ V2.4//EN" URI="http://devel.springer.de/A++/V2.4/DTD/A++V2.4.dtd" name="istex:docType"></istex:docType><istex:document><Publisher><PublisherInfo><PublisherName>Huazhong University of Science and Technology</PublisherName><PublisherLocation>Heidelberg</PublisherLocation><PublisherImprintName>Huazhong University of Science and Technology, co-published with Springer</PublisherImprintName></PublisherInfo><Journal OutputMedium="All"><JournalInfo JournalProductType="ArchiveJournal" NumberingStyle="Unnumbered"><JournalID>11596</JournalID><JournalPrintISSN>1672-0733</JournalPrintISSN><JournalElectronicISSN>1993-1352</JournalElectronicISSN><JournalTitle>Journal of Huazhong University of Science and Technology [Medical Sciences]</JournalTitle><JournalSubTitle>Medical Sciences</JournalSubTitle><JournalAbbreviatedTitle>J. Huazhong Univ. Sci. Technol. [Med. Sci.]</JournalAbbreviatedTitle><JournalSubjectGroup><JournalSubject Code="SCH" Type="Primary">Medicine & Public Health</JournalSubject><JournalSubject Code="SCH00007" Priority="1" Type="Secondary">Medicine/Public Health, general</JournalSubject><SubjectCollection Code="SC11">Medicine</SubjectCollection></JournalSubjectGroup></JournalInfo><Volume OutputMedium="All"><VolumeInfo TocLevels="0" VolumeType="Regular"><VolumeIDStart>33</VolumeIDStart><VolumeIDEnd>33</VolumeIDEnd><VolumeIssueCount>6</VolumeIssueCount></VolumeInfo><Issue IssueType="Regular" OutputMedium="All"><IssueInfo IssueType="Regular" TocLevels="0"><IssueIDStart>3</IssueIDStart><IssueIDEnd>3</IssueIDEnd><IssueArticleCount>25</IssueArticleCount><IssueHistory><OnlineDate><Year>2013</Year><Month>6</Month><Day>17</Day></OnlineDate><CoverDate><Year>2013</Year><Month>6</Month></CoverDate><PricelistYear>2013</PricelistYear></IssueHistory><IssueCopyright><CopyrightHolderName>Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg</CopyrightHolderName><CopyrightYear>2013</CopyrightYear></IssueCopyright></IssueInfo><Article ID="s11596-013-1124-9" OutputMedium="All"><ArticleInfo ArticleType="OriginalPaper" ContainsESM="No" Language="En" NumberingStyle="Unnumbered" TocLevels="0"><ArticleID>1124</ArticleID><ArticleDOI>10.1007/s11596-013-1124-9</ArticleDOI><ArticleSequenceNumber>8</ArticleSequenceNumber><ArticleTitle Language="En">Chloroquine relieves acute lung injury in rats with acute hemorrhagic necrotizing pancreatitis</ArticleTitle><ArticleFirstPage>357</ArticleFirstPage><ArticleLastPage>360</ArticleLastPage><ArticleHistory><RegistrationDate><Year>2013</Year><Month>6</Month><Day>16</Day></RegistrationDate><Received><Year>2012</Year><Month>12</Month><Day>14</Day></Received><OnlineDate><Year>2013</Year><Month>6</Month><Day>17</Day></OnlineDate></ArticleHistory><ArticleCopyright><CopyrightHolderName>Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg</CopyrightHolderName><CopyrightYear>2013</CopyrightYear></ArticleCopyright><ArticleGrants Type="Regular"><MetadataGrant Grant="OpenAccess"></MetadataGrant><AbstractGrant Grant="OpenAccess"></AbstractGrant><BodyPDFGrant Grant="Restricted"></BodyPDFGrant><BodyHTMLGrant Grant="Restricted"></BodyHTMLGrant><BibliographyGrant Grant="Restricted"></BibliographyGrant><ESMGrant Grant="Restricted"></ESMGrant></ArticleGrants></ArticleInfo><ArticleHeader><AuthorGroup><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Lei</GivenName><FamilyName>Zhang</FamilyName><NativeName>张 磊</NativeName></AuthorName><Contact><Email>zhangl@tjh.tjmu.edu.cn</Email></Contact></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Yan</GivenName><FamilyName>Chen</FamilyName><NativeName>陈 燕</NativeName></AuthorName></Author><Author AffiliationIDS="Aff2"><AuthorName DisplayOrder="Western"><GivenName>Lin</GivenName><FamilyName>Wang</FamilyName><NativeName>王 琳</NativeName></AuthorName></Author><Author AffiliationIDS="Aff1" CorrespondingAffiliationID="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Xiao-ping</GivenName><FamilyName>Chen</FamilyName><NativeName>陈孝平</NativeName></AuthorName><Contact><Email>chenxp@medmail.com.cn</Email></Contact></Author><Author AffiliationIDS="Aff1"><AuthorName DisplayOrder="Western"><GivenName>Wan-guang</GivenName><FamilyName>Zhang</FamilyName><NativeName>张万广</NativeName></AuthorName></Author><Author AffiliationIDS="Aff3"><AuthorName DisplayOrder="Western"><GivenName>Chun-you</GivenName><FamilyName>Wang</FamilyName><NativeName>王春友</NativeName></AuthorName></Author><Author AffiliationIDS="Aff3" CorrespondingAffiliationID="Aff3"><AuthorName DisplayOrder="Western"><GivenName>He-shui</GivenName><FamilyName>Wu</FamilyName><NativeName>吴河水</NativeName></AuthorName><Contact><Email>whs1898@public.wh.hb.cn</Email></Contact></Author><Affiliation ID="Aff1"><OrgDivision>Hepatic Surgery Center, Tongji Hospital, Tongji Medical College</OrgDivision><OrgName>Huazhong University of Science and Technology</OrgName><OrgAddress><City>Wuhan</City><Postcode>430030</Postcode><Country Code="CN">China</Country></OrgAddress></Affiliation><Affiliation ID="Aff2"><OrgDivision>Department of Pediatrics</OrgDivision><OrgName>Huazhong University of Science and Technology</OrgName><OrgAddress><City>Wuhan</City><Postcode>430022</Postcode><Country Code="CN">China</Country></OrgAddress></Affiliation><Affiliation ID="Aff3"><OrgDivision>Center of Pancreatic Surgery, Union Hospital, Tongji Medical College</OrgDivision><OrgName>Huazhong University of Science and Technology</OrgName><OrgAddress><City>Wuhan</City><Postcode>430022</Postcode><Country Code="CN">China</Country></OrgAddress></Affiliation></AuthorGroup><Abstract ID="Abs1" Language="En"><Heading>Summary</Heading><Para>This study preliminarily investigated the mechanism by which chloroquine (CQ) relieves acute lung injury (ALI) complicated in acute hemorrhagic necrotizing pancreatitis (AHNP). Sixty male Wistar rats were randomized into sham-operated group (group A, <Emphasis Type="Italic">n</Emphasis>=10), AHNP group (group B, <Emphasis Type="Italic">n</Emphasis>=10), <Emphasis Type="Italic">L</Emphasis>-arginine-treated group (group C, <Emphasis Type="Italic">n</Emphasis>=10), <Emphasis Type="Italic">L</Emphasis>-N-nitro-<Emphasis Type="Italic">L</Emphasis>-arginine methyl ester (NAME)-treated group (group D, <Emphasis Type="Italic">n</Emphasis>=10), CQ-treated group (group E, <Emphasis Type="Italic">n</Emphasis>=10) and CQ+<Emphasis Type="Italic">L</Emphasis>-NAME-treated group (group F, <Emphasis Type="Italic">n</Emphasis>=10). TLR4 expression was measured by using real time-PCR and Western blotting respectively. The results showed that, in the group B, the expression of TLR4 and the levels of TNF-α and IL-6 in the lungs were significantly increased, and the nitric oxide (NO) concentration was reduced, as compared with those in the group A (<Emphasis Type="Italic">P</Emphasis><0.05 or <Emphasis Type="Italic">P</Emphasis><0.01). Lung injury was aggravated with the increased expression of TLR4. When the inhibitor and stimulator of TLR4, namely <Emphasis Type="Italic">L</Emphasis>-Arg and <Emphasis Type="Italic">L</Emphasis>-NAME, were added respectively, lung injury was correspondingly relieved or aggravated (<Emphasis Type="Italic">P</Emphasis><0.05 or <Emphasis Type="Italic">P</Emphasis><0.01). In the group E, TLR4 expression was substantially lower and NO concentration higher than those in the group B (<Emphasis Type="Italic">P</Emphasis><0.05 or <Emphasis Type="Italic">P</Emphasis><0.01). However, in the group F, NO concentration was markedly decreased, and the inhibitory effect of CQ on TLR4 expression and the relief of lung injury were weakened when compared with those in the group E (<Emphasis Type="Italic">P</Emphasis><0.05 or <Emphasis Type="Italic">P</Emphasis><0.01). It was concluded that TLR4 may play an important role in the pathogenesis and development of ALI complicated in AHNP. CQ could relieve ALI by decreasing the TLR4 expression and increasing the NO release.</Para></Abstract><KeywordGroup Language="En"><Heading>Key words</Heading><Keyword>Toll-like receptor</Keyword><Keyword>acute hemorrhagic necrotizing pancreatitis</Keyword><Keyword>lung</Keyword><Keyword>chloroquine</Keyword><Keyword>nitric oxide</Keyword><Keyword><Emphasis Type="Italic">L</Emphasis>-arginine</Keyword><Keyword>N-nitro-<Emphasis Type="Italic">L</Emphasis>-arginine methyl ester</Keyword></KeywordGroup><ArticleNote Type="Misc"><SimplePara>These authors contributed equally to this work.</SimplePara></ArticleNote><ArticleNote Type="Misc"><SimplePara>This project was supported by the National Natural Science Foundation of China (No. 81201554).</SimplePara></ArticleNote></ArticleHeader><NoBody></NoBody></Article></Issue></Volume></Journal></Publisher></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Chloroquine relieves acute lung injury in rats with acute hemorrhagic necrotizing pancreatitis</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Chloroquine relieves acute lung injury in rats with acute hemorrhagic necrotizing pancreatitis</title></titleInfo><name type="personal"><namePart type="given">Lei</namePart><namePart type="family" transliteration="张 磊">Zhang</namePart><affiliation>Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China</affiliation><affiliation>E-mail: zhangl@tjh.tjmu.edu.cn</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Yan</namePart><namePart type="family" transliteration="陈 燕">Chen</namePart><affiliation>Department of Pediatrics, Huazhong University of Science and Technology, 430022, Wuhan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Lin</namePart><namePart type="family" transliteration="王 琳">Wang</namePart><affiliation>Department of Pediatrics, Huazhong University of Science and Technology, 430022, Wuhan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">Xiao-ping</namePart><namePart type="family" transliteration="陈孝平">Chen</namePart><affiliation>Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China</affiliation><affiliation>E-mail: chenxp@medmail.com.cn</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wan-guang</namePart><namePart type="family" transliteration="张万广">Zhang</namePart><affiliation>Hepatic Surgery Center, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430030, Wuhan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Chun-you</namePart><namePart type="family" transliteration="王春友">Wang</namePart><affiliation>Center of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal" displayLabel="corresp"><namePart type="given">He-shui</namePart><namePart type="family" transliteration="吴河水">Wu</namePart><affiliation>Center of Pancreatic Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 430022, Wuhan, China</affiliation><affiliation>E-mail: whs1898@public.wh.hb.cn</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="OriginalPaper" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>Huazhong University of Science and Technology</publisher><place><placeTerm type="text">Heidelberg</placeTerm></place><dateCreated encoding="w3cdtf">2012-12-14</dateCreated><dateIssued encoding="w3cdtf">2013-06-01</dateIssued><copyrightDate encoding="w3cdtf">2013</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><abstract lang="en">Summary: This study preliminarily investigated the mechanism by which chloroquine (CQ) relieves acute lung injury (ALI) complicated in acute hemorrhagic necrotizing pancreatitis (AHNP). Sixty male Wistar rats were randomized into sham-operated group (group A, n=10), AHNP group (group B, n=10), L-arginine-treated group (group C, n=10), L-N-nitro-L-arginine methyl ester (NAME)-treated group (group D, n=10), CQ-treated group (group E, n=10) and CQ+L-NAME-treated group (group F, n=10). TLR4 expression was measured by using real time-PCR and Western blotting respectively. The results showed that, in the group B, the expression of TLR4 and the levels of TNF-α and IL-6 in the lungs were significantly increased, and the nitric oxide (NO) concentration was reduced, as compared with those in the group A (P<0.05 or P<0.01). Lung injury was aggravated with the increased expression of TLR4. When the inhibitor and stimulator of TLR4, namely L-Arg and L-NAME, were added respectively, lung injury was correspondingly relieved or aggravated (P<0.05 or P<0.01). In the group E, TLR4 expression was substantially lower and NO concentration higher than those in the group B (P<0.05 or P<0.01). However, in the group F, NO concentration was markedly decreased, and the inhibitory effect of CQ on TLR4 expression and the relief of lung injury were weakened when compared with those in the group E (P<0.05 or P<0.01). It was concluded that TLR4 may play an important role in the pathogenesis and development of ALI complicated in AHNP. CQ could relieve ALI by decreasing the TLR4 expression and increasing the NO release.</abstract><subject lang="en"><genre>Key words</genre><topic>Toll-like receptor</topic><topic>acute hemorrhagic necrotizing pancreatitis</topic><topic>lung</topic><topic>chloroquine</topic><topic>nitric oxide</topic><topic>L -arginine</topic><topic>N-nitro- L -arginine methyl ester</topic></subject><relatedItem type="host"><titleInfo><title>Journal of Huazhong University of Science and Technology [Medical Sciences]</title><subTitle>Medical Sciences</subTitle></titleInfo><titleInfo type="abbreviated"><title>J. Huazhong Univ. Sci. Technol. [Med. Sci.]</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>Springer</publisher><dateIssued encoding="w3cdtf">2013-06-17</dateIssued><copyrightDate encoding="w3cdtf">2013</copyrightDate></originInfo><subject><genre>Journal-Subject-Collection</genre><topic authority="SpringerSubjectCodes" authorityURI="SC11">Medicine</topic></subject><subject><genre>Journal-Subject-Group</genre><topic authority="SpringerSubjectCodes" authorityURI="SCH">Medicine & Public Health</topic><topic authority="SpringerSubjectCodes" authorityURI="SCH00007">Medicine/Public Health, general</topic></subject><identifier type="ISSN">1672-0733</identifier><identifier type="eISSN">1993-1352</identifier><identifier type="JournalID">11596</identifier><identifier type="IssueArticleCount">25</identifier><identifier type="VolumeIssueCount">6</identifier><part><date>2013</date><detail type="volume"><number>33</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="pages"><start>357</start><end>360</end></extent></part><recordInfo><recordOrigin>Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg, 2013</recordOrigin></recordInfo></relatedItem><identifier type="istex">7BBD09EA85CFCCFC554668924D475360C3E84F29</identifier><identifier type="ark">ark:/67375/VQC-9GMKW13S-L</identifier><identifier type="DOI">10.1007/s11596-013-1124-9</identifier><identifier type="ArticleID">1124</identifier><identifier type="ArticleID">s11596-013-1124-9</identifier><accessCondition type="use and reproduction" contentType="copyright">Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg, 2013</accessCondition><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-3XSW68JL-F">springer</recordContentSource><recordOrigin>Huazhong University of Science and Technology and Springer-Verlag Berlin Heidelberg, 2013</recordOrigin></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/ark:/67375/VQC-9GMKW13S-L/record.json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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