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Activity of the plant flavanol quercetin in the mouse lymphoma L5178Y TK+/− mutation, DNA single-strand break, and Balb/c 3T3 chemical transformation assays

Identifieur interne : 000D21 ( Main/Merge ); précédent : 000D20; suivant : 000D22

Activity of the plant flavanol quercetin in the mouse lymphoma L5178Y TK+/− mutation, DNA single-strand break, and Balb/c 3T3 chemical transformation assays

Auteurs : Martin L. Meltz [États-Unis] ; James T. Macgregor [États-Unis]

Source :

Mutation Research/Genetic Toxicology [ 0165-1218 ] ; 1981.

RBID : ISTEX:70992B6B66D6FC2C8D9FBEB6D959F59F1A5BFDE1

English descriptors

Teeft :
- Activation system, Alkaline elution technique, Assay, Chemical transformation assay, Chemical transformation experiments, Clive, Dietary cause, Dmso, Elution rate, Higher concentrations, Human cancer, Mutant frequency, Mutation, Mutation assay, Mutation experiments, Quercetin, Same manner, Sigma chemical, Stock culture, Treatment interval, Viable cells.

Abstract

Abstract: The activity of quercetin was investigated in (a) the L5178Y TK+/− mutation assay system, using trifluorothymidine (TFT) as the selection agent; (b) the DNA single-strand break assay in L5178Y cells after the same treatment used for the mutation assay; and (c) the Balb/c 3T3 chemical transformation assay (foci method). Quercetin was active in the TK+/− mutation assay, increasing the frequency of TFT-resistant colonies from a control value of 37 per 106 viable cells to 355 per 106 viable cells at 20 μg/ml. When S9 was present, the activity was decreased at each concentration tested. As the S9 concentration employed (mg/ml protein) was decreased, the induced mutant frequency increased. DNA single-strand breakage was observed without S9 at 10 μg/ml, using the alkaline elution technique; a maximal rate of elution was reached at 20 μg/ml. In the chemical transformation experiments, transformation just at the level of 0.05% significance (if both intermediate and typical transformed colonies were combined) was observed. The evidence is sufficiently strong that additional attention should be given to its role as a dietary caused of human cancer.

Url:

https://api.istex.fr/document/70992B6B66D6FC2C8D9FBEB6D959F59F1A5BFDE1/fulltext/pdf

DOI: 10.1016/0165-1218(81)90043-4

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: The activity of quercetin was investigated in (a) the L5178Y TK+/− mutation assay system, using trifluorothymidine (TFT) as the selection agent; (b) the DNA single-strand break assay in L5178Y cells after the same treatment used for the mutation assay; and (c) the Balb/c 3T3 chemical transformation assay (foci method). Quercetin was active in the TK+/− mutation assay, increasing the frequency of TFT-resistant colonies from a control value of 37 per 106 viable cells to 355 per 106 viable cells at 20 μg/ml. When S9 was present, the activity was decreased at each concentration tested. As the S9 concentration employed (mg/ml protein) was decreased, the induced mutant frequency increased. DNA single-strand breakage was observed without S9 at 10 μg/ml, using the alkaline elution technique; a maximal rate of elution was reached at 20 μg/ml. In the chemical transformation experiments, transformation just at the level of 0.05% significance (if both intermediate and typical transformed colonies were combined) was observed. The evidence is sufficiently strong that additional attention should be given to its role as a dietary caused of human cancer.</div>
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Activity of the plant flavanol quercetin in the mouse lymphoma L5178Y TK+/− mutation, DNA single-strand break, and Balb/c 3T3 chemical transformation assays

Activity of the plant flavanol quercetin in the mouse lymphoma L5178Y TK+/− mutation, DNA single-strand break, and Balb/c 3T3 chemical transformation assays

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

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Pour mettre un lien sur cette page dans le réseau Wicri