Activity of the plant flavanol quercetin in the mouse lymphoma L5178Y TK+/− mutation, DNA single-strand break, and Balb/c 3T3 chemical transformation assays
Identifieur interne : 000B64 ( Istex/Corpus ); précédent : 000B63; suivant : 000B65Activity of the plant flavanol quercetin in the mouse lymphoma L5178Y TK+/− mutation, DNA single-strand break, and Balb/c 3T3 chemical transformation assays
Auteurs : Martin L. Meltz ; James T. MacgregorSource :
- Mutation Research/Genetic Toxicology [ 0165-1218 ] ; 1981.
English descriptors
- Teeft :
- Activation system, Alkaline elution technique, Assay, Chemical transformation assay, Chemical transformation experiments, Clive, Dietary cause, Dmso, Elution rate, Higher concentrations, Human cancer, Mutant frequency, Mutation, Mutation assay, Mutation experiments, Quercetin, Same manner, Sigma chemical, Stock culture, Treatment interval, Viable cells.
Abstract
Abstract: The activity of quercetin was investigated in (a) the L5178Y TK+/− mutation assay system, using trifluorothymidine (TFT) as the selection agent; (b) the DNA single-strand break assay in L5178Y cells after the same treatment used for the mutation assay; and (c) the Balb/c 3T3 chemical transformation assay (foci method). Quercetin was active in the TK+/− mutation assay, increasing the frequency of TFT-resistant colonies from a control value of 37 per 106 viable cells to 355 per 106 viable cells at 20 μg/ml. When S9 was present, the activity was decreased at each concentration tested. As the S9 concentration employed (mg/ml protein) was decreased, the induced mutant frequency increased. DNA single-strand breakage was observed without S9 at 10 μg/ml, using the alkaline elution technique; a maximal rate of elution was reached at 20 μg/ml. In the chemical transformation experiments, transformation just at the level of 0.05% significance (if both intermediate and typical transformed colonies were combined) was observed. The evidence is sufficiently strong that additional attention should be given to its role as a dietary caused of human cancer.
Url:
DOI: 10.1016/0165-1218(81)90043-4
Links to Exploration step
ISTEX:70992B6B66D6FC2C8D9FBEB6D959F59F1A5BFDE1Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Activity of the plant flavanol quercetin in the mouse lymphoma L5178Y TK+/− mutation, DNA single-strand break, and Balb/c 3T3 chemical transformation assays</title><author><name sortKey="Meltz, Martin L" sort="Meltz, Martin L" uniqKey="Meltz M" first="Martin L." last="Meltz">Martin L. Meltz</name><affiliation><mods:affiliation>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</mods:affiliation></affiliation><affiliation><mods:affiliation>Western Regional Research Center, U.S. Department of Agriculture, 800 Buchanan Street, Berkeley, CA 94710 U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Macgregor, James T" sort="Macgregor, James T" uniqKey="Macgregor J" first="James T." last="Macgregor">James T. Macgregor</name><affiliation><mods:affiliation>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:70992B6B66D6FC2C8D9FBEB6D959F59F1A5BFDE1</idno><date when="1981" year="1981">1981</date><idno type="doi">10.1016/0165-1218(81)90043-4</idno><idno type="url">https://api.istex.fr/document/70992B6B66D6FC2C8D9FBEB6D959F59F1A5BFDE1/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000B64</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000B64</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Activity of the plant flavanol quercetin in the mouse lymphoma L5178Y TK+/− mutation, DNA single-strand break, and Balb/c 3T3 chemical transformation assays</title><author><name sortKey="Meltz, Martin L" sort="Meltz, Martin L" uniqKey="Meltz M" first="Martin L." last="Meltz">Martin L. Meltz</name><affiliation><mods:affiliation>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</mods:affiliation></affiliation><affiliation><mods:affiliation>Western Regional Research Center, U.S. Department of Agriculture, 800 Buchanan Street, Berkeley, CA 94710 U.S.A.</mods:affiliation></affiliation></author><author><name sortKey="Macgregor, James T" sort="Macgregor, James T" uniqKey="Macgregor J" first="James T." last="Macgregor">James T. Macgregor</name><affiliation><mods:affiliation>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</mods:affiliation></affiliation><affiliation><mods:affiliation>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Mutation Research/Genetic Toxicology</title><title level="j" type="abbrev">MUTGEX</title><idno type="ISSN">0165-1218</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1981">1981</date><biblScope unit="volume">88</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="317">317</biblScope><biblScope unit="page" to="324">324</biblScope></imprint><idno type="ISSN">0165-1218</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0165-1218</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Activation system</term><term>Alkaline elution technique</term><term>Assay</term><term>Chemical transformation assay</term><term>Chemical transformation experiments</term><term>Clive</term><term>Dietary cause</term><term>Dmso</term><term>Elution rate</term><term>Higher concentrations</term><term>Human cancer</term><term>Mutant frequency</term><term>Mutation</term><term>Mutation assay</term><term>Mutation experiments</term><term>Quercetin</term><term>Same manner</term><term>Sigma chemical</term><term>Stock culture</term><term>Treatment interval</term><term>Viable cells</term></keywords></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Abstract: The activity of quercetin was investigated in (a) the L5178Y TK+/− mutation assay system, using trifluorothymidine (TFT) as the selection agent; (b) the DNA single-strand break assay in L5178Y cells after the same treatment used for the mutation assay; and (c) the Balb/c 3T3 chemical transformation assay (foci method). Quercetin was active in the TK+/− mutation assay, increasing the frequency of TFT-resistant colonies from a control value of 37 per 106 viable cells to 355 per 106 viable cells at 20 μg/ml. When S9 was present, the activity was decreased at each concentration tested. As the S9 concentration employed (mg/ml protein) was decreased, the induced mutant frequency increased. DNA single-strand breakage was observed without S9 at 10 μg/ml, using the alkaline elution technique; a maximal rate of elution was reached at 20 μg/ml. In the chemical transformation experiments, transformation just at the level of 0.05% significance (if both intermediate and typical transformed colonies were combined) was observed. The evidence is sufficiently strong that additional attention should be given to its role as a dietary caused of human cancer.</div></front></TEI><istex><corpusName>elsevier</corpusName><keywords><teeft><json:string>quercetin</json:string><json:string>mutation</json:string><json:string>dmso</json:string><json:string>assay</json:string><json:string>clive</json:string><json:string>mutation assay</json:string><json:string>viable cells</json:string><json:string>mutation experiments</json:string><json:string>chemical transformation experiments</json:string><json:string>dietary cause</json:string><json:string>chemical transformation assay</json:string><json:string>elution rate</json:string><json:string>same manner</json:string><json:string>mutant frequency</json:string><json:string>stock culture</json:string><json:string>activation system</json:string><json:string>sigma chemical</json:string><json:string>alkaline elution technique</json:string><json:string>treatment interval</json:string><json:string>higher concentrations</json:string><json:string>human cancer</json:string></teeft></keywords><author><json:item><name>Martin L. Meltz</name><affiliations><json:string>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</json:string><json:string>Western Regional Research Center, U.S. Department of Agriculture, 800 Buchanan Street, Berkeley, CA 94710 U.S.A.</json:string></affiliations></json:item><json:item><name>James T. MacGregor</name><affiliations><json:string>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</json:string><json:string>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</json:string></affiliations></json:item></author><arkIstex>ark:/67375/6H6-WJF4XW4C-F</arkIstex><language><json:string>eng</json:string></language><originalGenre><json:string>Full-length article</json:string></originalGenre><abstract>Abstract: The activity of quercetin was investigated in (a) the L5178Y TK+/− mutation assay system, using trifluorothymidine (TFT) as the selection agent; (b) the DNA single-strand break assay in L5178Y cells after the same treatment used for the mutation assay; and (c) the Balb/c 3T3 chemical transformation assay (foci method). Quercetin was active in the TK+/− mutation assay, increasing the frequency of TFT-resistant colonies from a control value of 37 per 106 viable cells to 355 per 106 viable cells at 20 μg/ml. When S9 was present, the activity was decreased at each concentration tested. As the S9 concentration employed (mg/ml protein) was decreased, the induced mutant frequency increased. DNA single-strand breakage was observed without S9 at 10 μg/ml, using the alkaline elution technique; a maximal rate of elution was reached at 20 μg/ml. In the chemical transformation experiments, transformation just at the level of 0.05% significance (if both intermediate and typical transformed colonies were combined) was observed. The evidence is sufficiently strong that additional attention should be given to its role as a dietary caused of human cancer.</abstract><qualityIndicators><score>9.148</score><pdfWordCount>5822</pdfWordCount><pdfCharCount>18387</pdfCharCount><pdfVersion>1.2</pdfVersion><pdfPageCount>8</pdfPageCount><pdfPageSize>447 x 684 pts</pdfPageSize><refBibsNative>true</refBibsNative><abstractWordCount>179</abstractWordCount><abstractCharCount>1165</abstractCharCount><keywordCount>0</keywordCount></qualityIndicators><title>Activity of the plant flavanol quercetin in the mouse lymphoma L5178Y TK+/− mutation, DNA single-strand break, and Balb/c 3T3 chemical transformation assays</title><pmid><json:string>7254225</json:string></pmid><pii><json:string>0165-1218(81)90043-4</json:string></pii><genre><json:string>research-article</json:string></genre><serie><title>I. Application to genetic toxicology testing</title><language><json:string>unknown</json:string></language><pages><first>391</first><last>406</last></pages></serie><host><title>Mutation Research/Genetic Toxicology</title><language><json:string>unknown</json:string></language><publicationDate>1981</publicationDate><issn><json:string>0165-1218</json:string></issn><pii><json:string>S0165-1218(00)X0045-6</json:string></pii><volume>88</volume><issue>3</issue><pages><first>317</first><last>324</last></pages><genre><json:string>journal</json:string></genre></host><namedEntities><unitex><date><json:string>1981</json:string></date><geogName></geogName><orgName><json:string>W I T H AND WITHOUT AN ADDED METABOLIC ACTIVATION SYSTEM Chemical</json:string><json:string>Department of Agriculture</json:string><json:string>Western Regional Research Center</json:string><json:string>Southwest Foundation for Research and Education</json:string><json:string>Ciba-Geigy Corp.</json:string><json:string>National Cancer Institute</json:string><json:string>Sigma Chemical Co.</json:string></orgName><orgName_funder></orgName_funder><orgName_provider></orgName_provider><persName><json:string>Kohn</json:string><json:string>G.D. Manners</json:string><json:string>Douglas Winters</json:string><json:string>Takeo Kakunaga</json:string><json:string>Thomas Goodwin</json:string><json:string>Nancy Jo</json:string><json:string>Clive</json:string></persName><placeName><json:string>NY</json:string><json:string>Berkeley</json:string><json:string>Ardsley</json:string></placeName><ref_url></ref_url><ref_bibl><json:string>Bjeldanes and Chang, 1977</json:string><json:string>Umezawa et al., 1977</json:string><json:string>Maruta et al., 1979</json:string><json:string>Kohn et al., 1974, 1976</json:string><json:string>Seino et al., 1978</json:string><json:string>Clive and Spector, 1975</json:string><json:string>Kastenbaum and Bowman, 1970</json:string><json:string>Brown and Dietrich, 1979</json:string><json:string>Ambrose et al., 1952</json:string><json:string>Hardigree and Epler, 1978</json:string><json:string>Brown, 1980</json:string><json:string>Brown and Dietrich, 1978</json:string><json:string>Kakunaga, 1973</json:string><json:string>Fornace and Kohn, 1976</json:string><json:string>Umezawa et al. (1977)</json:string><json:string>Sugimura et al., 1977</json:string><json:string>Hermann, 1976</json:string><json:string>Pamukcu et al., 1980</json:string><json:string>Clive et al., 1979</json:string><json:string>MacGregor and Jurd, 1978</json:string><json:string>R Department of Radiology, The Universityof Texas Health Science Center, 7703</json:string><json:string>Boyland et a l , 1964</json:string><json:string>Ames et al.</json:string><json:string>Clive et al., 1973</json:string><json:string>Amacher et al., 1979</json:string><json:string>Kuhnau, 1976</json:string></ref_bibl><bibl></bibl></unitex></namedEntities><ark><json:string>ark:/67375/6H6-WJF4XW4C-F</json:string></ark><categories><wos></wos><scienceMetrix></scienceMetrix><scopus><json:string>1 - Life Sciences</json:string><json:string>2 - Biochemistry, Genetics and Molecular Biology</json:string><json:string>3 - Genetics</json:string><json:string>1 - Life Sciences</json:string><json:string>2 - Pharmacology, Toxicology and Pharmaceutics</json:string><json:string>3 - Toxicology</json:string></scopus><inist><json:string>1 - sciences appliquees, technologies et medecines</json:string><json:string>2 - sciences biologiques et medicales</json:string><json:string>3 - sciences medicales</json:string></inist></categories><publicationDate>1981</publicationDate><copyrightDate>1981</copyrightDate><doi><json:string>10.1016/0165-1218(81)90043-4</json:string></doi><id>70992B6B66D6FC2C8D9FBEB6D959F59F1A5BFDE1</id><score>1</score><fulltext><json:item><extension>pdf</extension><original>true</original><mimetype>application/pdf</mimetype><uri>https://api.istex.fr/document/70992B6B66D6FC2C8D9FBEB6D959F59F1A5BFDE1/fulltext/pdf</uri></json:item><json:item><extension>zip</extension><original>false</original><mimetype>application/zip</mimetype><uri>https://api.istex.fr/document/70992B6B66D6FC2C8D9FBEB6D959F59F1A5BFDE1/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/70992B6B66D6FC2C8D9FBEB6D959F59F1A5BFDE1/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Activity of the plant flavanol quercetin in the mouse lymphoma L5178Y TK+/− mutation, DNA single-strand break, and Balb/c 3T3 chemical transformation assays</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1981</date></publicationStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Activity of the plant flavanol quercetin in the mouse lymphoma L5178Y TK+/− mutation, DNA single-strand break, and Balb/c 3T3 chemical transformation assays</title><author xml:id="author-0000"><persName><forename type="first">Martin L.</forename><surname>Meltz</surname></persName><affiliation>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</affiliation><affiliation>Western Regional Research Center, U.S. Department of Agriculture, 800 Buchanan Street, Berkeley, CA 94710 U.S.A.</affiliation></author><author xml:id="author-0001"><persName><forename type="first">James T.</forename><surname>MacGregor</surname></persName><affiliation>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</affiliation><affiliation>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</affiliation></author><idno type="istex">70992B6B66D6FC2C8D9FBEB6D959F59F1A5BFDE1</idno><idno type="DOI">10.1016/0165-1218(81)90043-4</idno><idno type="PII">0165-1218(81)90043-4</idno></analytic><monogr><title level="j">Mutation Research/Genetic Toxicology</title><title level="j" type="abbrev">MUTGEX</title><idno type="pISSN">0165-1218</idno><idno type="PII">S0165-1218(00)X0045-6</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1981"></date><biblScope unit="volume">88</biblScope><biblScope unit="issue">3</biblScope><biblScope unit="page" from="317">317</biblScope><biblScope unit="page" to="324">324</biblScope></imprint></monogr></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1981</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The activity of quercetin was investigated in (a) the L5178Y TK+/− mutation assay system, using trifluorothymidine (TFT) as the selection agent; (b) the DNA single-strand break assay in L5178Y cells after the same treatment used for the mutation assay; and (c) the Balb/c 3T3 chemical transformation assay (foci method). Quercetin was active in the TK+/− mutation assay, increasing the frequency of TFT-resistant colonies from a control value of 37 per 106 viable cells to 355 per 106 viable cells at 20 μg/ml. When S9 was present, the activity was decreased at each concentration tested. As the S9 concentration employed (mg/ml protein) was decreased, the induced mutant frequency increased. DNA single-strand breakage was observed without S9 at 10 μg/ml, using the alkaline elution technique; a maximal rate of elution was reached at 20 μg/ml. In the chemical transformation experiments, transformation just at the level of 0.05% significance (if both intermediate and typical transformed colonies were combined) was observed. The evidence is sufficiently strong that additional attention should be given to its role as a dietary caused of human cancer.</p></abstract></profileDesc><revisionDesc><change when="1980-07-28">Modified</change><change when="1981">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/70992B6B66D6FC2C8D9FBEB6D959F59F1A5BFDE1/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>MUTGEX</jid><aid>81900434</aid><ce:pii>0165-1218(81)90043-4</ce:pii><ce:doi>10.1016/0165-1218(81)90043-4</ce:doi><ce:copyright type="unknown" year="1981"></ce:copyright></item-info><head><ce:title>Activity of the plant flavanol quercetin in the mouse lymphoma L5178Y TK<ce:sup>+/−</ce:sup> mutation, DNA single-strand break, and Balb/c 3T3 chemical transformation assays</ce:title><ce:author-group><ce:author><ce:given-name>Martin L.</ce:given-name><ce:surname>Meltz</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup>b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>James T.</ce:given-name><ce:surname>MacGregor</ce:surname><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF1"><ce:sup>a</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>Western Regional Research Center, U.S. Department of Agriculture, 800 Buchanan Street, Berkeley, CA 94710 U.S.A.</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="8" month="5" year="1980"></ce:date-received><ce:date-revised day="28" month="7" year="1980"></ce:date-revised><ce:date-accepted day="25" month="8" year="1980"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The activity of quercetin was investigated in (a) the L5178Y TK<ce:sup>+/−</ce:sup> mutation assay system, using trifluorothymidine (TFT) as the selection agent; (b) the DNA single-strand break assay in L5178Y cells after the same treatment used for the mutation assay; and (c) the Balb/c 3T3 chemical transformation assay (foci method). Quercetin was active in the TK<ce:sup>+/−</ce:sup> mutation assay, increasing the frequency of TFT-resistant colonies from a control value of 37 per 10<ce:sup>6</ce:sup> viable cells to 355 per 10<ce:sup>6</ce:sup> viable cells at 20 μg/ml. When S9 was present, the activity was decreased at each concentration tested. As the S9 concentration employed (mg/ml protein) was decreased, the induced mutant frequency increased. DNA single-strand breakage was observed without S9 at 10 μg/ml, using the alkaline elution technique; a maximal rate of elution was reached at 20 μg/ml. In the chemical transformation experiments, transformation just at the level of 0.05% significance (if both intermediate and typical transformed colonies were combined) was observed. The evidence is sufficiently strong that additional attention should be given to its role as a dietary caused of human cancer.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Activity of the plant flavanol quercetin in the mouse lymphoma L5178Y TK+/− mutation, DNA single-strand break, and Balb/c 3T3 chemical transformation assays</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Activity of the plant flavanol quercetin in the mouse lymphoma L5178Y TK</title></titleInfo><name type="personal"><namePart type="given">Martin L.</namePart><namePart type="family">Meltz</namePart><affiliation>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</affiliation><affiliation>Western Regional Research Center, U.S. Department of Agriculture, 800 Buchanan Street, Berkeley, CA 94710 U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">James T.</namePart><namePart type="family">MacGregor</namePart><affiliation>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</affiliation><affiliation>Department of Radiology, The University of Texas Health Science Center, 7703 Floyd Curl Drive, San Antonio, TX 78284, U.S.A.</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1981</dateIssued><dateModified encoding="w3cdtf">1980-07-28</dateModified><copyrightDate encoding="w3cdtf">1981</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The activity of quercetin was investigated in (a) the L5178Y TK+/− mutation assay system, using trifluorothymidine (TFT) as the selection agent; (b) the DNA single-strand break assay in L5178Y cells after the same treatment used for the mutation assay; and (c) the Balb/c 3T3 chemical transformation assay (foci method). Quercetin was active in the TK+/− mutation assay, increasing the frequency of TFT-resistant colonies from a control value of 37 per 106 viable cells to 355 per 106 viable cells at 20 μg/ml. When S9 was present, the activity was decreased at each concentration tested. As the S9 concentration employed (mg/ml protein) was decreased, the induced mutant frequency increased. DNA single-strand breakage was observed without S9 at 10 μg/ml, using the alkaline elution technique; a maximal rate of elution was reached at 20 μg/ml. In the chemical transformation experiments, transformation just at the level of 0.05% significance (if both intermediate and typical transformed colonies were combined) was observed. The evidence is sufficiently strong that additional attention should be given to its role as a dietary caused of human cancer.</abstract><relatedItem type="host"><titleInfo><title>Mutation Research/Genetic Toxicology</title></titleInfo><titleInfo type="abbreviated"><title>MUTGEX</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">198103</dateIssued></originInfo><identifier type="ISSN">0165-1218</identifier><identifier type="PII">S0165-1218(00)X0045-6</identifier><part><date>198103</date><detail type="volume"><number>88</number><caption>vol.</caption></detail><detail type="issue"><number>3</number><caption>no.</caption></detail><extent unit="issue-pages"><start>233</start><end>324</end></extent><extent unit="pages"><start>317</start><end>324</end></extent></part></relatedItem><identifier type="istex">70992B6B66D6FC2C8D9FBEB6D959F59F1A5BFDE1</identifier><identifier type="ark">ark:/67375/6H6-WJF4XW4C-F</identifier><identifier type="DOI">10.1016/0165-1218(81)90043-4</identifier><identifier type="PII">0165-1218(81)90043-4</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/70992B6B66D6FC2C8D9FBEB6D959F59F1A5BFDE1/metadata/json</uri></json:item></metadata></istex></record>Pour manipuler ce document sous Unix (Dilib)
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