Synergistic increases in IL-1 synthesis by the human monocytic cell line THP-1 treated with PAF and endotoxin
Identifieur interne : 000A95 ( Main/Merge ); précédent : 000A94; suivant : 000A96Synergistic increases in IL-1 synthesis by the human monocytic cell line THP-1 treated with PAF and endotoxin
Auteurs : Roger A. Barthelson [États-Unis] ; Thomas Potter [États-Unis] ; Frank H. Valone [États-Unis]Source :
- Cellular Immunology [ 0008-8749 ] ; 1990.
English descriptors
- Teeft :
- Academic press, Active form, Assay, Autoradiograph, Barthelson, Bioassay, Buffer control, Cell immunology, Cell proteins, Cell supernatants, Collaborative research, Complex effects, Cytokine release, Densitometric measurement, Different concentrations, Different pair, Elisa, Escherichia coli, Human monocytes, Human monocytic leukemia cell line, Immunol, Microtiter plates, Monoclonal antibodies, Monocyte, Palo alto, Precursor, Proliferation assay, Rabbit antiserum, Radioimmune assay, Relative staining, Releaseby, Separate series, Sigma chemical, Significant increase, Small difference, Stable cell line, Supernatant, Syntex corp, Total absorbance, Valone.
Abstract
Abstract: The capacity to stimulate cytokine release may be important to the long-term effects of platelet-activating factor (PAF), which has a very short half-life. Previous studies have shown that PAF stimulates interleukin 1 (IL-1) release by human monocytes. IL-1 and other cytokines produced in response to PAF may be important to the long-term effects of this short-lived lipid. The THP-1 human monocytic leukemia cell line, was used to study the mechanism by which PAF stimulates IL-1 release. PAF stimulates the release of IL-1 β activity into THP-1 cell supernatants with a multiphasic dose-response curve very similar to that for monocytes. When THP-1 cells are treated with PAF and LPS in combination, these two stimuli interact synergistically to greatly increase the release of IL-1 activity. To assess the effect of PAF on IL-1 β synthesis, THP-1 cell pellet proteins were separated by SDS-PAGE, blotted, and immunostained to detect IL-1β. Immunostaining revealed that PAF increases intracellular IL-1 β precursor and that the combination of PAF and LPS increases IL-1 β precursor synergistically. PAF increases IL-1 β release mainly by increasing IL-1 β synthesis.
Url:
DOI: 10.1016/0008-8749(90)90069-4
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Previous studies have shown that PAF stimulates interleukin 1 (IL-1) release by human monocytes. IL-1 and other cytokines produced in response to PAF may be important to the long-term effects of this short-lived lipid. The THP-1 human monocytic leukemia cell line, was used to study the mechanism by which PAF stimulates IL-1 release. PAF stimulates the release of IL-1 β activity into THP-1 cell supernatants with a multiphasic dose-response curve very similar to that for monocytes. When THP-1 cells are treated with PAF and LPS in combination, these two stimuli interact synergistically to greatly increase the release of IL-1 activity. To assess the effect of PAF on IL-1 β synthesis, THP-1 cell pellet proteins were separated by SDS-PAGE, blotted, and immunostained to detect IL-1β. Immunostaining revealed that PAF increases intracellular IL-1 β precursor and that the combination of PAF and LPS increases IL-1 β precursor synergistically. PAF increases IL-1 β release mainly by increasing IL-1 β synthesis.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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