Synergistic increases in IL-1 synthesis by the human monocytic cell line THP-1 treated with PAF and endotoxin
Identifieur interne : 000918 ( Istex/Corpus ); précédent : 000917; suivant : 000919Synergistic increases in IL-1 synthesis by the human monocytic cell line THP-1 treated with PAF and endotoxin
Auteurs : Roger A. Barthelson ; Thomas Potter ; Frank H. ValoneSource :
- Cellular Immunology [ 0008-8749 ] ; 1990.
English descriptors
- Teeft :
- Academic press, Active form, Assay, Autoradiograph, Barthelson, Bioassay, Buffer control, Cell immunology, Cell proteins, Cell supernatants, Collaborative research, Complex effects, Cytokine release, Densitometric measurement, Different concentrations, Different pair, Elisa, Escherichia coli, Human monocytes, Human monocytic leukemia cell line, Immunol, Microtiter plates, Monoclonal antibodies, Monocyte, Palo alto, Precursor, Proliferation assay, Rabbit antiserum, Radioimmune assay, Relative staining, Releaseby, Separate series, Sigma chemical, Significant increase, Small difference, Stable cell line, Supernatant, Syntex corp, Total absorbance, Valone.
Abstract
Abstract: The capacity to stimulate cytokine release may be important to the long-term effects of platelet-activating factor (PAF), which has a very short half-life. Previous studies have shown that PAF stimulates interleukin 1 (IL-1) release by human monocytes. IL-1 and other cytokines produced in response to PAF may be important to the long-term effects of this short-lived lipid. The THP-1 human monocytic leukemia cell line, was used to study the mechanism by which PAF stimulates IL-1 release. PAF stimulates the release of IL-1 β activity into THP-1 cell supernatants with a multiphasic dose-response curve very similar to that for monocytes. When THP-1 cells are treated with PAF and LPS in combination, these two stimuli interact synergistically to greatly increase the release of IL-1 activity. To assess the effect of PAF on IL-1 β synthesis, THP-1 cell pellet proteins were separated by SDS-PAGE, blotted, and immunostained to detect IL-1β. Immunostaining revealed that PAF increases intracellular IL-1 β precursor and that the combination of PAF and LPS increases IL-1 β precursor synergistically. PAF increases IL-1 β release mainly by increasing IL-1 β synthesis.
Url:
DOI: 10.1016/0008-8749(90)90069-4
Links to Exploration step
ISTEX:86E1DF7906750C6C813B33AC7C8CCAF813C35A4CLe document en format XML
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Valone</name><affiliation><mods:affiliation>Department of Medicine, Veterans Administration Medical Center, 111k, 4150 Clement Street, San Francisco, California 94121 USA</mods:affiliation></affiliation><affiliation><mods:affiliation>The University of California, San Francisco, California 94143 USA</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:86E1DF7906750C6C813B33AC7C8CCAF813C35A4C</idno><date when="1990" year="1990">1990</date><idno type="doi">10.1016/0008-8749(90)90069-4</idno><idno type="url">https://api.istex.fr/document/86E1DF7906750C6C813B33AC7C8CCAF813C35A4C/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000918</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000918</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Synergistic increases in IL-1 synthesis by the human monocytic cell line THP-1 treated with PAF and endotoxin</title><author><name sortKey="Barthelson, Roger A" sort="Barthelson, Roger A" uniqKey="Barthelson R" first="Roger A." last="Barthelson">Roger A. 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Previous studies have shown that PAF stimulates interleukin 1 (IL-1) release by human monocytes. IL-1 and other cytokines produced in response to PAF may be important to the long-term effects of this short-lived lipid. The THP-1 human monocytic leukemia cell line, was used to study the mechanism by which PAF stimulates IL-1 release. PAF stimulates the release of IL-1 β activity into THP-1 cell supernatants with a multiphasic dose-response curve very similar to that for monocytes. When THP-1 cells are treated with PAF and LPS in combination, these two stimuli interact synergistically to greatly increase the release of IL-1 activity. To assess the effect of PAF on IL-1 β synthesis, THP-1 cell pellet proteins were separated by SDS-PAGE, blotted, and immunostained to detect IL-1β. Immunostaining revealed that PAF increases intracellular IL-1 β precursor and that the combination of PAF and LPS increases IL-1 β precursor synergistically. 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The THP-1 human monocytic leukemia cell line, was used to study the mechanism by which PAF stimulates IL-1 release. PAF stimulates the release of IL-1 β activity into THP-1 cell supernatants with a multiphasic dose-response curve very similar to that for monocytes. When THP-1 cells are treated with PAF and LPS in combination, these two stimuli interact synergistically to greatly increase the release of IL-1 activity. To assess the effect of PAF on IL-1 β synthesis, THP-1 cell pellet proteins were separated by SDS-PAGE, blotted, and immunostained to detect IL-1β. Immunostaining revealed that PAF increases intracellular IL-1 β precursor and that the combination of PAF and LPS increases IL-1 β precursor synergistically. 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Previous studies have shown that PAF stimulates interleukin 1 (IL-1) release by human monocytes. IL-1 and other cytokines produced in response to PAF may be important to the long-term effects of this short-lived lipid. The THP-1 human monocytic leukemia cell line, was used to study the mechanism by which PAF stimulates IL-1 release. PAF stimulates the release of IL-1 β activity into THP-1 cell supernatants with a multiphasic dose-response curve very similar to that for monocytes. When THP-1 cells are treated with PAF and LPS in combination, these two stimuli interact synergistically to greatly increase the release of IL-1 activity. To assess the effect of PAF on IL-1 β synthesis, THP-1 cell pellet proteins were separated by SDS-PAGE, blotted, and immunostained to detect IL-1β. Immunostaining revealed that PAF increases intracellular IL-1 β precursor and that the combination of PAF and LPS increases IL-1 β precursor synergistically. PAF increases IL-1 β release mainly by increasing IL-1 β synthesis.</p></abstract></profileDesc><revisionDesc><change when="1990">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/86E1DF7906750C6C813B33AC7C8CCAF813C35A4C/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="UTF-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla" xml:lang="en"><item-info><jid>YCIMM</jid><aid>90900694</aid><ce:pii>0008-8749(90)90069-4</ce:pii><ce:doi>10.1016/0008-8749(90)90069-4</ce:doi><ce:copyright type="unknown" year="1990"></ce:copyright></item-info><head><ce:title>Synergistic increases in IL-1 synthesis by the human monocytic cell line THP-1 treated with PAF and endotoxin</ce:title><ce:author-group><ce:author><ce:given-name>Roger A.</ce:given-name><ce:surname>Barthelson</ce:surname><ce:cross-ref refid="AFF1"><ce:sup loc="post">a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup loc="post">b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Thomas</ce:given-name><ce:surname>Potter</ce:surname><ce:cross-ref refid="AFF1"><ce:sup loc="post">a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup loc="post">b</ce:sup></ce:cross-ref></ce:author><ce:author><ce:given-name>Frank H.</ce:given-name><ce:surname>Valone</ce:surname><ce:cross-ref refid="AFF1"><ce:sup loc="post">a</ce:sup></ce:cross-ref><ce:cross-ref refid="AFF2"><ce:sup loc="post">b</ce:sup></ce:cross-ref></ce:author><ce:affiliation id="AFF1"><ce:label>a</ce:label><ce:textfn>Department of Medicine, Veterans Administration Medical Center, 111k, 4150 Clement Street, San Francisco, California 94121 USA</ce:textfn></ce:affiliation><ce:affiliation id="AFF2"><ce:label>b</ce:label><ce:textfn>The University of California, San Francisco, California 94143 USA</ce:textfn></ce:affiliation></ce:author-group><ce:date-received day="29" month="6" year="1989"></ce:date-received><ce:date-accepted day="13" month="9" year="1989"></ce:date-accepted><ce:abstract class="author"><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para view="all" id="simple-para.0010">The capacity to stimulate cytokine release may be important to the long-term effects of platelet-activating factor (PAF), which has a very short half-life. Previous studies have shown that PAF stimulates interleukin 1 (IL-1) release by human monocytes. IL-1 and other cytokines produced in response to PAF may be important to the long-term effects of this short-lived lipid. The THP-1 human monocytic leukemia cell line, was used to study the mechanism by which PAF stimulates IL-1 release. PAF stimulates the release of IL-1 β activity into THP-1 cell supernatants with a multiphasic dose-response curve very similar to that for monocytes. When THP-1 cells are treated with PAF and LPS in combination, these two stimuli interact synergistically to greatly increase the release of IL-1 activity. To assess the effect of PAF on IL-1 β synthesis, THP-1 cell pellet proteins were separated by SDS-PAGE, blotted, and immunostained to detect IL-1β. Immunostaining revealed that PAF increases intracellular IL-1 β precursor and that the combination of PAF and LPS increases IL-1 β precursor synergistically. PAF increases IL-1 β release mainly by increasing IL-1 β synthesis.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Synergistic increases in IL-1 synthesis by the human monocytic cell line THP-1 treated with PAF and endotoxin</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Synergistic increases in IL-1 synthesis by the human monocytic cell line THP-1 treated with PAF and endotoxin</title></titleInfo><name type="personal"><namePart type="given">Roger A.</namePart><namePart type="family">Barthelson</namePart><affiliation>Department of Medicine, Veterans Administration Medical Center, 111k, 4150 Clement Street, San Francisco, California 94121 USA</affiliation><affiliation>The University of California, San Francisco, California 94143 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Thomas</namePart><namePart type="family">Potter</namePart><affiliation>Department of Medicine, Veterans Administration Medical Center, 111k, 4150 Clement Street, San Francisco, California 94121 USA</affiliation><affiliation>The University of California, San Francisco, California 94143 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Frank H.</namePart><namePart type="family">Valone</namePart><affiliation>Department of Medicine, Veterans Administration Medical Center, 111k, 4150 Clement Street, San Francisco, California 94121 USA</affiliation><affiliation>The University of California, San Francisco, California 94143 USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article" authority="ISTEX" authorityURI="https://content-type.data.istex.fr" valueURI="https://content-type.data.istex.fr/ark:/67375/XTP-1JC4F85T-7">research-article</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1990</dateIssued><copyrightDate encoding="w3cdtf">1990</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><abstract lang="en">Abstract: The capacity to stimulate cytokine release may be important to the long-term effects of platelet-activating factor (PAF), which has a very short half-life. Previous studies have shown that PAF stimulates interleukin 1 (IL-1) release by human monocytes. IL-1 and other cytokines produced in response to PAF may be important to the long-term effects of this short-lived lipid. The THP-1 human monocytic leukemia cell line, was used to study the mechanism by which PAF stimulates IL-1 release. PAF stimulates the release of IL-1 β activity into THP-1 cell supernatants with a multiphasic dose-response curve very similar to that for monocytes. When THP-1 cells are treated with PAF and LPS in combination, these two stimuli interact synergistically to greatly increase the release of IL-1 activity. To assess the effect of PAF on IL-1 β synthesis, THP-1 cell pellet proteins were separated by SDS-PAGE, blotted, and immunostained to detect IL-1β. Immunostaining revealed that PAF increases intracellular IL-1 β precursor and that the combination of PAF and LPS increases IL-1 β precursor synergistically. PAF increases IL-1 β release mainly by increasing IL-1 β synthesis.</abstract><relatedItem type="host"><titleInfo><title>Cellular Immunology</title></titleInfo><titleInfo type="abbreviated"><title>YCIMM</title></titleInfo><genre type="journal" authority="ISTEX" authorityURI="https://publication-type.data.istex.fr" valueURI="https://publication-type.data.istex.fr/ark:/67375/JMC-0GLKJH51-B">journal</genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">199001</dateIssued></originInfo><identifier type="ISSN">0008-8749</identifier><identifier type="PII">S0008-8749(00)X0294-8</identifier><part><date>199001</date><detail type="volume"><number>125</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue-pages"><start>1</start><end>272</end></extent><extent unit="pages"><start>142</start><end>150</end></extent></part></relatedItem><identifier type="istex">86E1DF7906750C6C813B33AC7C8CCAF813C35A4C</identifier><identifier type="ark">ark:/67375/6H6-9VH08SWM-6</identifier><identifier type="DOI">10.1016/0008-8749(90)90069-4</identifier><identifier type="PII">0008-8749(90)90069-4</identifier><identifier type="ArticleID">90900694</identifier><recordInfo><recordContentSource authority="ISTEX" authorityURI="https://loaded-corpus.data.istex.fr" valueURI="https://loaded-corpus.data.istex.fr/ark:/67375/XBH-HKKZVM7B-M">elsevier</recordContentSource></recordInfo></mods><json:item><extension>json</extension><original>false</original><mimetype>application/json</mimetype><uri>https://api.istex.fr/document/86E1DF7906750C6C813B33AC7C8CCAF813C35A4C/metadata/json</uri></json:item></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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