A 74-bp Promoter of the Tnp2 Gene Confers Testis- and Spermatid-Specific Expression in Transgenic Mice
Identifieur interne : 000385 ( Main/Curation ); précédent : 000384; suivant : 000386A 74-bp Promoter of the Tnp2 Gene Confers Testis- and Spermatid-Specific Expression in Transgenic Mice
Auteurs : Özlem Topaloglu [Allemagne] ; Gregor Schlüter [Allemagne] ; Karim Nayernia [Allemagne] ; Wolfgang Engel [Allemagne]Source :
- Biochemical and Biophysical Research Communications [ 0006-291X ] ; 2001.
English descriptors
Abstract
During the final stages of spermatogenesis, round spermatids undergo several morphological, biochemical, and physiological modifications which result in the formation of mature spermatozoa. One of these is the nuclear condensation, achieved by the replacement of somatic-type and testis-specific histones by transition proteins and protamines leading to cessation of transcription several days before the completion of spermiogenesis. Therefore, a strict temporal and stage-specific gene expression is necessary for the correct differentiation of round spermatids into mature spermatozoa. In this study, the 5′ regulatory region of rat Tnp2 gene was investigated by primer extension analysis and transgenic mice study. Primer extension analysis revealed a transcription start site which lies 70 bp upstream of the translation start codon. By transgenic mice studies, we demonstrated that a 147-bp 5′ untranslated region corresponding to the region −74 to +73 is sufficient to confer testis- and spermatid-specific expression of rat Tnp2 gene.
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DOI: 10.1006/bbrc.2001.5999
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xml:lang="en">During the final stages of spermatogenesis, round spermatids undergo several morphological, biochemical, and physiological modifications which result in the formation of mature spermatozoa. One of these is the nuclear condensation, achieved by the replacement of somatic-type and testis-specific histones by transition proteins and protamines leading to cessation of transcription several days before the completion of spermiogenesis. Therefore, a strict temporal and stage-specific gene expression is necessary for the correct differentiation of round spermatids into mature spermatozoa. In this study, the 5′ regulatory region of rat Tnp2 gene was investigated by primer extension analysis and transgenic mice study. Primer extension analysis revealed a transcription start site which lies 70 bp upstream of the translation start codon. By transgenic mice studies, we demonstrated that a 147-bp 5′ untranslated region corresponding to the region −74 to +73 is sufficient to confer testis- and spermatid-specific expression of rat Tnp2 gene.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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