Rat transition nuclear protein 2 regulatory region directs haploid expression of reporter gene in male germ cells of transgenic mice
Identifieur interne : 000870 ( Main/Corpus ); précédent : 000869; suivant : 000871Rat transition nuclear protein 2 regulatory region directs haploid expression of reporter gene in male germ cells of transgenic mice
Auteurs : Karim Nayernia ; Detlef Böhm ; Özlem Topaloglu ; Gregor Schlüter ; Wolfgang EngelSource :
- Molecular Reproduction and Development [ 1040-452X ] ; 2001-04-01.
English descriptors
Abstract
During spermiogenesis, the successive replacement of somatic histones by basic proteins, the transition nuclear proteins and protamines, allows normal sperm condensation. Transition nuclear protein 2 (TNP2) is transcribed postmeiotically in round spermatids. In order to determine regulatory flanking sequences responsible for stage specific expression of TNP2 gene, different transgenic mice were generated by microinjection of fertilized eggs. We demonstrate here that 525 bp of 5′‐ and 920 bp of 3′‐flanking sequences of rat TNP2 gene could properly and efficiently direct chloramphenicol acetyltransferase gene expression to the postmeiotic male germ cells of transgenic mice. During male germ cell differentiation the first transgene transcripts were observed in round spermatids and translation started 6 days later in elongating spermatids, which is an evidence for posttranscriptional regulation of transgene expression. In contrast, transgenic mice bearing only the 525 bp 5′‐flanking sequences of rat transition protein 2 gene and 3′‐flanking sequences of the simian virus 40 large T antigen showed low levels of transgene expression in testis. From these results, it can be concluded that the 525 bp 5′‐flanking sequences regulate the cell specific expression and the sequences located in 920 bp 3′‐flanking region either enhance the transgene expression in the male germ cells or may have a posttranscriptional role in stabilizing the mRNA in addition to its function in delaying the mRNA translation. Using comparative alignment of 5′‐flanking of TNP2 genes from different species, the putative regulatory sequences are identified. Mol. Reprod. Dev. 58:368–375, 2001. © 2001 Wiley‐Liss, Inc.
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DOI: 10.1002/1098-2795(20010401)58:4<368::AID-MRD3>3.0.CO;2-J
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Transition nuclear protein 2 (TNP2) is transcribed postmeiotically in round spermatids. In order to determine regulatory flanking sequences responsible for stage specific expression of TNP2 gene, different transgenic mice were generated by microinjection of fertilized eggs. We demonstrate here that 525 bp of 5′‐ and 920 bp of 3′‐flanking sequences of rat TNP2 gene could properly and efficiently direct chloramphenicol acetyltransferase gene expression to the postmeiotic male germ cells of transgenic mice. During male germ cell differentiation the first transgene transcripts were observed in round spermatids and translation started 6 days later in elongating spermatids, which is an evidence for posttranscriptional regulation of transgene expression. In contrast, transgenic mice bearing only the 525 bp 5′‐flanking sequences of rat transition protein 2 gene and 3′‐flanking sequences of the simian virus 40 large T antigen showed low levels of transgene expression in testis. From these results, it can be concluded that the 525 bp 5′‐flanking sequences regulate the cell specific expression and the sequences located in 920 bp 3′‐flanking region either enhance the transgene expression in the male germ cells or may have a posttranscriptional role in stabilizing the mRNA in addition to its function in delaying the mRNA translation. Using comparative alignment of 5′‐flanking of TNP2 genes from different species, the putative regulatory sequences are identified. Mol. Reprod. Dev. 58:368–375, 2001. © 2001 Wiley‐Liss, Inc.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Karim Nayernia</name><affiliations><json:string>Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D‐37073 Göttingen, Germany</json:string></affiliations></json:item><json:item><name>Detlef Böhm</name><affiliations><json:string>Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D‐37073 Göttingen, Germany</json:string></affiliations></json:item><json:item><name>Özlem Topaloglu</name><affiliations><json:string>Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D‐37073 Göttingen, Germany</json:string></affiliations></json:item><json:item><name>Gregor Schlüter</name><affiliations><json:string>Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D‐37073 Göttingen, Germany</json:string></affiliations></json:item><json:item><name>Wolfgang Engel</name><affiliations><json:string>Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D‐37073 Göttingen, Germany</json:string></affiliations></json:item></author><subject><json:item><lang><json:string>eng</json:string></lang><value>transgenic mice</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>haploid expression</value></json:item><json:item><lang><json:string>eng</json:string></lang><value>transition protein 2</value></json:item></subject><language><json:string>eng</json:string></language><abstract>During spermiogenesis, the successive replacement of somatic histones by basic proteins, the transition nuclear proteins and protamines, allows normal sperm condensation. Transition nuclear protein 2 (TNP2) is transcribed postmeiotically in round spermatids. In order to determine regulatory flanking sequences responsible for stage specific expression of TNP2 gene, different transgenic mice were generated by microinjection of fertilized eggs. We demonstrate here that 525 bp of 5′‐ and 920 bp of 3′‐flanking sequences of rat TNP2 gene could properly and efficiently direct chloramphenicol acetyltransferase gene expression to the postmeiotic male germ cells of transgenic mice. During male germ cell differentiation the first transgene transcripts were observed in round spermatids and translation started 6 days later in elongating spermatids, which is an evidence for posttranscriptional regulation of transgene expression. In contrast, transgenic mice bearing only the 525 bp 5′‐flanking sequences of rat transition protein 2 gene and 3′‐flanking sequences of the simian virus 40 large T antigen showed low levels of transgene expression in testis. From these results, it can be concluded that the 525 bp 5′‐flanking sequences regulate the cell specific expression and the sequences located in 920 bp 3′‐flanking region either enhance the transgene expression in the male germ cells or may have a posttranscriptional role in stabilizing the mRNA in addition to its function in delaying the mRNA translation. Using comparative alignment of 5′‐flanking of TNP2 genes from different species, the putative regulatory sequences are identified. Mol. Reprod. 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Böhm contributed equally to this work.</note><note>Deutsche Forschungsgemeinschaft - No. SFB 271;</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a" type="main" xml:lang="en">Rat transition nuclear protein 2 regulatory region directs haploid expression of reporter gene in male germ cells of transgenic mice</title><author><persName><forename type="first">Karim</forename><surname>Nayernia</surname></persName><affiliation>Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D‐37073 Göttingen, Germany</affiliation></author><author><persName><forename type="first">Detlef</forename><surname>Böhm</surname></persName><affiliation>Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D‐37073 Göttingen, Germany</affiliation></author><author><persName><forename type="first">Özlem</forename><surname>Topaloglu</surname></persName><affiliation>Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D‐37073 Göttingen, Germany</affiliation></author><author><persName><forename type="first">Gregor</forename><surname>Schlüter</surname></persName><affiliation>Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D‐37073 Göttingen, Germany</affiliation></author><author><persName><forename type="first">Wolfgang</forename><surname>Engel</surname></persName><note type="correspondence"><p>Correspondence: Institute of Human Genetics, Heinrich‐Düker‐Weg 12, D‐37073 Göttingen, Germany.</p></note><affiliation>Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D‐37073 Göttingen, Germany</affiliation></author></analytic><monogr><title level="j">Molecular Reproduction and Development</title><title level="j" type="sub">Incorporating Gamete Research</title><title level="j" type="abbrev">Mol. 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Transition nuclear protein 2 (TNP2) is transcribed postmeiotically in round spermatids. In order to determine regulatory flanking sequences responsible for stage specific expression of TNP2 gene, different transgenic mice were generated by microinjection of fertilized eggs. We demonstrate here that 525 bp of 5′‐ and 920 bp of 3′‐flanking sequences of rat TNP2 gene could properly and efficiently direct chloramphenicol acetyltransferase gene expression to the postmeiotic male germ cells of transgenic mice. During male germ cell differentiation the first transgene transcripts were observed in round spermatids and translation started 6 days later in elongating spermatids, which is an evidence for posttranscriptional regulation of transgene expression. In contrast, transgenic mice bearing only the 525 bp 5′‐flanking sequences of rat transition protein 2 gene and 3′‐flanking sequences of the simian virus 40 large T antigen showed low levels of transgene expression in testis. From these results, it can be concluded that the 525 bp 5′‐flanking sequences regulate the cell specific expression and the sequences located in 920 bp 3′‐flanking region either enhance the transgene expression in the male germ cells or may have a posttranscriptional role in stabilizing the mRNA in addition to its function in delaying the mRNA translation. Using comparative alignment of 5′‐flanking of TNP2 genes from different species, the putative regulatory sequences are identified. Mol. Reprod. 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In order to determine regulatory flanking sequences responsible for stage specific expression of <i>TNP2</i> gene, different transgenic mice were generated by microinjection of fertilized eggs. We demonstrate here that 525 bp of 5′‐ and 920 bp of 3′‐flanking sequences of rat <i>TNP2</i> gene could properly and efficiently direct <i>chloramphenicol acetyltransferase</i> gene expression to the postmeiotic male germ cells of transgenic mice. During male germ cell differentiation the first transgene transcripts were observed in round spermatids and translation started 6 days later in elongating spermatids, which is an evidence for posttranscriptional regulation of transgene expression. In contrast, transgenic mice bearing only the 525 bp 5′‐flanking sequences of rat transition protein 2 gene and 3′‐flanking sequences of the simian virus 40 large T antigen showed low levels of transgene expression in testis. From these results, it can be concluded that the 525 bp 5′‐flanking sequences regulate the cell specific expression and the sequences located in 920 bp 3′‐flanking region either enhance the transgene expression in the male germ cells or may have a posttranscriptional role in stabilizing the mRNA in addition to its function in delaying the mRNA translation. Using comparative alignment of 5′‐flanking of <i>TNP2</i> genes from different species, the putative regulatory sequences are identified. <i>Mol. Reprod. Dev.</i> 58:368–375, 2001. © 2001 Wiley‐Liss, Inc.</p></abstract></abstractGroup></contentMeta><noteGroup><note xml:id="fn1"><label>*</label><p>K. Nayernia and D. Böhm contributed equally to this work.</p></note></noteGroup></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Rat transition nuclear protein 2 regulatory region directs haploid expression of reporter gene in male germ cells of transgenic mice</title></titleInfo><titleInfo type="abbreviated" lang="en"><title>Transition Protein 2 Transgenic Mice</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Rat transition nuclear protein 2 regulatory region directs haploid expression of reporter gene in male germ cells of transgenic mice</title></titleInfo><name type="personal"><namePart type="given">Karim</namePart><namePart type="family">Nayernia</namePart><affiliation>Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D‐37073 Göttingen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Detlef</namePart><namePart type="family">Böhm</namePart><affiliation>Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D‐37073 Göttingen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Özlem</namePart><namePart type="family">Topaloglu</namePart><affiliation>Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D‐37073 Göttingen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Gregor</namePart><namePart type="family">Schlüter</namePart><affiliation>Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D‐37073 Göttingen, Germany</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wolfgang</namePart><namePart type="family">Engel</namePart><affiliation>Institute of Human Genetics, University of Göttingen, Heinrich Düker Weg 12, D‐37073 Göttingen, Germany</affiliation><description>Correspondence: Institute of Human Genetics, Heinrich‐Düker‐Weg 12, D‐37073 Göttingen, Germany.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>John Wiley & Sons, Inc.</publisher><place><placeTerm type="text">New York</placeTerm></place><dateIssued encoding="w3cdtf">2001-04-01</dateIssued><dateCaptured encoding="w3cdtf">2000-08-18</dateCaptured><dateValid encoding="w3cdtf">2000-10-27</dateValid><copyrightDate encoding="w3cdtf">2001</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="figures">9</extent><extent unit="references">29</extent><extent unit="words">1066</extent></physicalDescription><abstract lang="en">During spermiogenesis, the successive replacement of somatic histones by basic proteins, the transition nuclear proteins and protamines, allows normal sperm condensation. Transition nuclear protein 2 (TNP2) is transcribed postmeiotically in round spermatids. In order to determine regulatory flanking sequences responsible for stage specific expression of TNP2 gene, different transgenic mice were generated by microinjection of fertilized eggs. We demonstrate here that 525 bp of 5′‐ and 920 bp of 3′‐flanking sequences of rat TNP2 gene could properly and efficiently direct chloramphenicol acetyltransferase gene expression to the postmeiotic male germ cells of transgenic mice. During male germ cell differentiation the first transgene transcripts were observed in round spermatids and translation started 6 days later in elongating spermatids, which is an evidence for posttranscriptional regulation of transgene expression. In contrast, transgenic mice bearing only the 525 bp 5′‐flanking sequences of rat transition protein 2 gene and 3′‐flanking sequences of the simian virus 40 large T antigen showed low levels of transgene expression in testis. From these results, it can be concluded that the 525 bp 5′‐flanking sequences regulate the cell specific expression and the sequences located in 920 bp 3′‐flanking region either enhance the transgene expression in the male germ cells or may have a posttranscriptional role in stabilizing the mRNA in addition to its function in delaying the mRNA translation. Using comparative alignment of 5′‐flanking of TNP2 genes from different species, the putative regulatory sequences are identified. Mol. Reprod. Dev. 58:368–375, 2001. © 2001 Wiley‐Liss, Inc.</abstract><note type="content">*K. Nayernia and D. Böhm contributed equally to this work.</note><note type="funding">Deutsche Forschungsgemeinschaft - No. SFB 271; </note><subject lang="en"><genre>Keywords</genre><topic>transgenic mice</topic><topic>haploid expression</topic><topic>transition protein 2</topic></subject><relatedItem type="host"><titleInfo><title>Molecular Reproduction and Development</title><subTitle>Incorporating Gamete Research</subTitle></titleInfo><titleInfo type="abbreviated"><title>Mol. Reprod. Dev.</title></titleInfo><genre type="Journal">journal</genre><subject><genre>article category</genre><topic>Transgenics</topic></subject><identifier type="ISSN">1040-452X</identifier><identifier type="eISSN">1098-2795</identifier><identifier type="DOI">10.1002/(ISSN)1098-2795</identifier><identifier type="PublisherID">MRD</identifier><part><date>2001</date><detail type="volume"><caption>vol.</caption><number>58</number></detail><detail type="issue"><caption>no.</caption><number>4</number></detail><extent unit="pages"><start>368</start><end>375</end><total>8</total></extent></part></relatedItem><identifier type="istex">5EA824ACCB35120D40EBABF18E254E01A1273CA1</identifier><identifier type="DOI">10.1002/1098-2795(20010401)58:4<368::AID-MRD3>3.0.CO;2-J</identifier><identifier type="ArticleID">MRD3</identifier><accessCondition type="use and reproduction" contentType="copyright">Copyright © 2001 Wiley‐Liss, Inc.</accessCondition><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>John Wiley & Sons, Inc.</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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