A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.
Identifieur interne : 000128 ( PubMed/Checkpoint ); précédent : 000127; suivant : 000129A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.
Auteurs : Nikko P. Torres [Canada] ; Grant W. BrownSource :
- Methods in molecular biology (Clifton, N.J.) ; 2015.
English descriptors
- KwdEn :
- MESH :
- cytology : Saccharomyces cerevisiae.
- growth & development : Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae.
- methods : Microscopy, Confocal, Microscopy, Fluorescence.
- Cells, Cultured, DNA Replication, Imaging, Three-Dimensional, Stress, Physiological, Time Factors.
Abstract
High-throughput imaging of yeast cells expressing fluorescent proteins can be used to understand biological pathways in the context of spatial organization. Here we describe a method for imaging yeast cells expressing proteins tagged with green fluorescent protein (GFP) and/or red fluorescent protein (RFP), with or without drug treatment, in a 384-well format, using the PerkinElmer Opera high-content confocal imaging microscope.
DOI: 10.1007/978-1-4939-2596-4_1
PubMed: 25916702
Affiliations:
Links toward previous steps (curation, corpus...)
Links to Exploration step
pubmed:25916702Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.</title><author><name sortKey="Torres, Nikko P" sort="Torres, Nikko P" uniqKey="Torres N" first="Nikko P" last="Torres">Nikko P. Torres</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry and Donnelly Centre, University of Toronto, 160 College Street, Toronto, ON, Canada, M5S 3E1.</nlm:affiliation><orgName type="university">Université de Toronto</orgName><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Brown, Grant W" sort="Brown, Grant W" uniqKey="Brown G" first="Grant W" last="Brown">Grant W. Brown</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2015">2015</date><idno type="doi">10.1007/978-1-4939-2596-4_1</idno><idno type="RBID">pubmed:25916702</idno><idno type="pmid">25916702</idno><idno type="wicri:Area/PubMed/Corpus">000084</idno><idno type="wicri:Area/PubMed/Curation">000084</idno><idno type="wicri:Area/PubMed/Checkpoint">000128</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.</title><author><name sortKey="Torres, Nikko P" sort="Torres, Nikko P" uniqKey="Torres N" first="Nikko P" last="Torres">Nikko P. Torres</name><affiliation wicri:level="4"><nlm:affiliation>Department of Biochemistry and Donnelly Centre, University of Toronto, 160 College Street, Toronto, ON, Canada, M5S 3E1.</nlm:affiliation><orgName type="university">Université de Toronto</orgName><country>Canada</country><placeName><settlement type="city">Toronto</settlement><region type="state">Ontario</region></placeName></affiliation></author><author><name sortKey="Brown, Grant W" sort="Brown, Grant W" uniqKey="Brown G" first="Grant W" last="Brown">Grant W. Brown</name></author></analytic><series><title level="j">Methods in molecular biology (Clifton, N.J.)</title><idno type="e-ISSN">1940-6029</idno><imprint><date when="2015" type="published">2015</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cells, Cultured</term><term>DNA Replication</term><term>Imaging, Three-Dimensional</term><term>Microscopy, Confocal (methods)</term><term>Microscopy, Fluorescence (methods)</term><term>Saccharomyces cerevisiae (cytology)</term><term>Saccharomyces cerevisiae (growth & development)</term><term>Saccharomyces cerevisiae (metabolism)</term><term>Stress, Physiological</term><term>Time Factors</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microscopy, Confocal</term><term>Microscopy, Fluorescence</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term><term>DNA Replication</term><term>Imaging, Three-Dimensional</term><term>Stress, Physiological</term><term>Time Factors</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">High-throughput imaging of yeast cells expressing fluorescent proteins can be used to understand biological pathways in the context of spatial organization. Here we describe a method for imaging yeast cells expressing proteins tagged with green fluorescent protein (GFP) and/or red fluorescent protein (RFP), with or without drug treatment, in a 384-well format, using the PerkinElmer Opera high-content confocal imaging microscope.</div></front></TEI><pubmed><MedlineCitation Owner="NLM" Status="MEDLINE"><PMID Version="1">25916702</PMID><DateCreated><Year>2015</Year><Month>04</Month><Day>28</Day></DateCreated><DateCompleted><Year>2016</Year><Month>01</Month><Day>18</Day></DateCompleted><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1940-6029</ISSN><JournalIssue CitedMedium="Internet"><Volume>1300</Volume><PubDate><Year>2015</Year></PubDate></JournalIssue><Title>Methods in molecular biology (Clifton, N.J.)</Title><ISOAbbreviation>Methods Mol. Biol.</ISOAbbreviation></Journal><ArticleTitle>A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.</ArticleTitle><Pagination><MedlinePgn>1-12</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/978-1-4939-2596-4_1</ELocationID><Abstract><AbstractText>High-throughput imaging of yeast cells expressing fluorescent proteins can be used to understand biological pathways in the context of spatial organization. Here we describe a method for imaging yeast cells expressing proteins tagged with green fluorescent protein (GFP) and/or red fluorescent protein (RFP), with or without drug treatment, in a 384-well format, using the PerkinElmer Opera high-content confocal imaging microscope.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Torres</LastName><ForeName>Nikko P</ForeName><Initials>NP</Initials><AffiliationInfo><Affiliation>Department of Biochemistry and Donnelly Centre, University of Toronto, 160 College Street, Toronto, ON, Canada, M5S 3E1.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Brown</LastName><ForeName>Grant W</ForeName><Initials>GW</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Methods Mol Biol</MedlineTA><NlmUniqueID>9214969</NlmUniqueID><ISSNLinking>1064-3745</ISSNLinking></MedlineJournalInfo><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName MajorTopicYN="N" UI="D002478">Cells, Cultured</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y" UI="D004261">DNA Replication</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D021621">Imaging, Three-Dimensional</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D018613">Microscopy, Confocal</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000379">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D008856">Microscopy, Fluorescence</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000379">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D012441">Saccharomyces cerevisiae</DescriptorName><QualifierName MajorTopicYN="Y" UI="Q000166">cytology</QualifierName><QualifierName MajorTopicYN="N" UI="Q000254">growth & development</QualifierName><QualifierName MajorTopicYN="Y" UI="Q000378">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="Y" UI="D013312">Stress, Physiological</DescriptorName></MeshHeading><MeshHeading><DescriptorName MajorTopicYN="N" UI="D013997">Time Factors</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2015</Year><Month>4</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2015</Year><Month>4</Month><Day>29</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2016</Year><Month>1</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="doi">10.1007/978-1-4939-2596-4_1</ArticleId><ArticleId IdType="pubmed">25916702</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country><region><li>Ontario</li></region><settlement><li>Toronto</li></settlement><orgName><li>Université de Toronto</li></orgName></list><tree><noCountry><name sortKey="Brown, Grant W" sort="Brown, Grant W" uniqKey="Brown G" first="Grant W" last="Brown">Grant W. Brown</name></noCountry><country name="Canada"><region name="Ontario"><name sortKey="Torres, Nikko P" sort="Torres, Nikko P" uniqKey="Torres N" first="Nikko P" last="Torres">Nikko P. Torres</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Musique/explor/OperaV1/Data/PubMed/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000128 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd -nk 000128 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Musique
|area= OperaV1
|flux= PubMed
|étape= Checkpoint
|type= RBID
|clé= pubmed:25916702
|texte= A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/PubMed/Checkpoint/RBID.i -Sk "pubmed:25916702" \
| HfdSelect -Kh $EXPLOR_AREA/Data/PubMed/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a OperaV1
| This area was generated with Dilib version V0.6.21. |  |