A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.
Identifieur interne : 000084 ( PubMed/Corpus ); précédent : 000083; suivant : 000085A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.
Auteurs : Nikko P. Torres ; Grant W. BrownSource :
- Methods in molecular biology (Clifton, N.J.) ; 2015.
English descriptors
- KwdEn :
- MESH :
- cytology : Saccharomyces cerevisiae.
- growth & development : Saccharomyces cerevisiae.
- metabolism : Saccharomyces cerevisiae.
- methods : Microscopy, Confocal, Microscopy, Fluorescence.
- Cells, Cultured, DNA Replication, Imaging, Three-Dimensional, Stress, Physiological, Time Factors.
Abstract
High-throughput imaging of yeast cells expressing fluorescent proteins can be used to understand biological pathways in the context of spatial organization. Here we describe a method for imaging yeast cells expressing proteins tagged with green fluorescent protein (GFP) and/or red fluorescent protein (RFP), with or without drug treatment, in a 384-well format, using the PerkinElmer Opera high-content confocal imaging microscope.
DOI: 10.1007/978-1-4939-2596-4_1
PubMed: 25916702
Links to Exploration step
pubmed:25916702Le document en format XML
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<author><name sortKey="Torres, Nikko P" sort="Torres, Nikko P" uniqKey="Torres N" first="Nikko P" last="Torres">Nikko P. Torres</name>
<affiliation><nlm:affiliation>Department of Biochemistry and Donnelly Centre, University of Toronto, 160 College Street, Toronto, ON, Canada, M5S 3E1.</nlm:affiliation>
</affiliation>
</author>
<author><name sortKey="Brown, Grant W" sort="Brown, Grant W" uniqKey="Brown G" first="Grant W" last="Brown">Grant W. Brown</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.</title>
<author><name sortKey="Torres, Nikko P" sort="Torres, Nikko P" uniqKey="Torres N" first="Nikko P" last="Torres">Nikko P. Torres</name>
<affiliation><nlm:affiliation>Department of Biochemistry and Donnelly Centre, University of Toronto, 160 College Street, Toronto, ON, Canada, M5S 3E1.</nlm:affiliation>
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<author><name sortKey="Brown, Grant W" sort="Brown, Grant W" uniqKey="Brown G" first="Grant W" last="Brown">Grant W. Brown</name>
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<series><title level="j">Methods in molecular biology (Clifton, N.J.)</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cells, Cultured</term>
<term>DNA Replication</term>
<term>Imaging, Three-Dimensional</term>
<term>Microscopy, Confocal (methods)</term>
<term>Microscopy, Fluorescence (methods)</term>
<term>Saccharomyces cerevisiae (cytology)</term>
<term>Saccharomyces cerevisiae (growth & development)</term>
<term>Saccharomyces cerevisiae (metabolism)</term>
<term>Stress, Physiological</term>
<term>Time Factors</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Saccharomyces cerevisiae</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Microscopy, Confocal</term>
<term>Microscopy, Fluorescence</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Cells, Cultured</term>
<term>DNA Replication</term>
<term>Imaging, Three-Dimensional</term>
<term>Stress, Physiological</term>
<term>Time Factors</term>
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<front><div type="abstract" xml:lang="en">High-throughput imaging of yeast cells expressing fluorescent proteins can be used to understand biological pathways in the context of spatial organization. Here we describe a method for imaging yeast cells expressing proteins tagged with green fluorescent protein (GFP) and/or red fluorescent protein (RFP), with or without drug treatment, in a 384-well format, using the PerkinElmer Opera high-content confocal imaging microscope.</div>
</front>
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<DateCreated><Year>2015</Year>
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<Day>28</Day>
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<DateCompleted><Year>2016</Year>
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<JournalIssue CitedMedium="Internet"><Volume>1300</Volume>
<PubDate><Year>2015</Year>
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<Title>Methods in molecular biology (Clifton, N.J.)</Title>
<ISOAbbreviation>Methods Mol. Biol.</ISOAbbreviation>
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<ArticleTitle>A high-throughput confocal fluorescence microscopy platform to study DNA replication stress in yeast cells.</ArticleTitle>
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<Abstract><AbstractText>High-throughput imaging of yeast cells expressing fluorescent proteins can be used to understand biological pathways in the context of spatial organization. Here we describe a method for imaging yeast cells expressing proteins tagged with green fluorescent protein (GFP) and/or red fluorescent protein (RFP), with or without drug treatment, in a 384-well format, using the PerkinElmer Opera high-content confocal imaging microscope.</AbstractText>
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<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Torres</LastName>
<ForeName>Nikko P</ForeName>
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<AffiliationInfo><Affiliation>Department of Biochemistry and Donnelly Centre, University of Toronto, 160 College Street, Toronto, ON, Canada, M5S 3E1.</Affiliation>
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<MeshHeading><DescriptorName MajorTopicYN="Y" UI="D013312">Stress, Physiological</DescriptorName>
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<MeshHeading><DescriptorName MajorTopicYN="N" UI="D013997">Time Factors</DescriptorName>
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