An egg injection method for assessing early life stage mortality of polychlorinated dibenzo-p-dioxins, dibenzofurans, and biphenyls in rainbow trout, (Oncorhynchus mykiss)
Identifieur interne : 003586 ( Main/Merge ); précédent : 003585; suivant : 003587An egg injection method for assessing early life stage mortality of polychlorinated dibenzo-p-dioxins, dibenzofurans, and biphenyls in rainbow trout, (Oncorhynchus mykiss)
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Abstract
To characterize the risk that polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and biphenyls (PCBs) pose to salmonid early life stage survival, we developed a method to expose rainbow trout (Oncorhynchus mykiss) eggs to graded doses of PCDD, PCDF, and PCB congeners, using 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as a prototype. Rainbow trout eggs were injected 24–50 h post-fertil ization with 0.2 μl of 50 mM phosphatidylcholine (PC) liposomes (control) or 0.2 μl of 5–7 graded doses of TCDD incorporated into 50 mM PC liposomes. Injection volume never exceeded 0.6% egg volume. Immediately following injection, the injection site was sealed with Super glueR, resulting in 92–97% of TCDD dose retained by the egg. Following both egg injection and waterborne egg exposure. TCDD toxicity in rainbow trout was manifested by half-hatching mortality but predominantly by sac fry mortality associated with hemorrhages, pericardial edema, and yolk sac edema. TCDD LD50s, following injection and waterborne exposure of rainbow trout eggs, were 421 (331–489) and 439 (346–519) pg TCDD/g egg (LD50, 95% fiducial limits), respectively. As in rainbow trout, TCDD toxicity in lake trout (Salvelinus namaycush) following the same two routes of exposure was manifested by half-hatching mortality but predominantly by sac fry mortality preceded by hemorrhages and yolk sac edema. LD50s, based on the dose of TCDD in lake trout eggs, were 47 (21–65) and 65 (60–71) pg/g following injection and waterborne exposure, respectively. The egg injection method is ideal for assessing the relationship between early life stage mortality in rainbow trout and graded egg doses of individual PCDD, PCDF, or PCB congeners.
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DOI: 10.1016/0166-445X(92)90033-J
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">An egg injection method for assessing early life stage mortality of polychlorinated dibenzo-p-dioxins, dibenzofurans, and biphenyls in rainbow trout, (Oncorhynchus mykiss)</title><author><name sortKey="Walker, Mary K" uniqKey="Walker M">Mary K. Walker</name><affiliation wicri:level="1"><mods:affiliation>Environmental Toxicology Center University of Wisconsin, Madison, Wisconsin, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Environmental Toxicology Center University of Wisconsin, Madison, Wisconsin</wicri:regionArea><wicri:noRegion>Wisconsin</wicri:noRegion></affiliation><affiliation wicri:level="2"><mods:affiliation>Present address: U.S. Fish and Wildlife Service, P.O. Box 818, National Fisheries Research Center. LaCrosse, WI 54602, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Present address: U.S. Fish and Wildlife Service, P.O. Box 818, National Fisheries Research Center. LaCrosse, WI 54602</wicri:regionArea><placeName><region type="state">Wisconsin</region></placeName></affiliation></author><author><name sortKey="Hufnagle Jr, Lawrence C" uniqKey="Hufnagle Jr L">Lawrence C. Hufnagle, Jr.</name><affiliation wicri:level="1"><mods:affiliation>School of Pharmacy University of Wisconsin, Madison, Wisconsin, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Pharmacy University of Wisconsin, Madison, Wisconsin</wicri:regionArea><wicri:noRegion>Wisconsin</wicri:noRegion></affiliation></author><author><name sortKey="Clayton, Murray K" uniqKey="Clayton M">Murray K. Clayton</name><affiliation wicri:level="1"><mods:affiliation>Departments of Statistics and Plant Pathology: University of Wisconsin, Madison, Wisconsin, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Departments of Statistics and Plant Pathology: University of Wisconsin, Madison, Wisconsin</wicri:regionArea><wicri:noRegion>Wisconsin</wicri:noRegion></affiliation></author><author><name sortKey="Peterson, Richard E" uniqKey="Peterson R">Richard E. Peterson</name><affiliation wicri:level="2"><mods:affiliation>Correspondence to: R.E. Peterson, School of Pharmacy, 425 N. Charter St., University of Wisconsin, Madison, WI 53706, U.S.A.</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Correspondence to: R.E. Peterson, School of Pharmacy, 425 N. Charter St., University of Wisconsin, Madison, WI 53706</wicri:regionArea><placeName><region type="state">Wisconsin</region></placeName></affiliation><affiliation wicri:level="1"><mods:affiliation>Environmental Toxicology Center University of Wisconsin, Madison, Wisconsin, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Environmental Toxicology Center University of Wisconsin, Madison, Wisconsin</wicri:regionArea><wicri:noRegion>Wisconsin</wicri:noRegion></affiliation><affiliation wicri:level="1"><mods:affiliation>School of Pharmacy University of Wisconsin, Madison, Wisconsin, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>School of Pharmacy University of Wisconsin, Madison, Wisconsin</wicri:regionArea><wicri:noRegion>Wisconsin</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="RBID">ISTEX:ECE7E904E4C3A54ACBF5C2B75F91AD53540CE77F</idno><date when="1992">1992</date><idno type="doi">10.1016/0166-445X(92)90033-J</idno><idno type="url">https://api.istex.fr/document/ECE7E904E4C3A54ACBF5C2B75F91AD53540CE77F/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">003496</idno><idno type="wicri:Area/Istex/Curation">003496</idno><idno type="wicri:Area/Istex/Checkpoint">002A28</idno><idno type="MainMerge">002A28</idno><idno type="wicri:Area/Main/Merge">003586</idno></publicationStmt><seriesStmt><idno type="ISSN">0166-445X</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>2,3,7,8-Tetrachlorodibenzo-p-dioxin</term><term>Early life stage</term><term>Microinjection</term><term>PCB</term><term>Rainbow trout</term><term>TCDD</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="eng">To characterize the risk that polychlorinated dibenzo-p-dioxins (PCDDs), dibenzofurans (PCDFs), and biphenyls (PCBs) pose to salmonid early life stage survival, we developed a method to expose rainbow trout (Oncorhynchus mykiss) eggs to graded doses of PCDD, PCDF, and PCB congeners, using 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) as a prototype. Rainbow trout eggs were injected 24–50 h post-fertil ization with 0.2 μl of 50 mM phosphatidylcholine (PC) liposomes (control) or 0.2 μl of 5–7 graded doses of TCDD incorporated into 50 mM PC liposomes. Injection volume never exceeded 0.6% egg volume. Immediately following injection, the injection site was sealed with Super glueR, resulting in 92–97% of TCDD dose retained by the egg. Following both egg injection and waterborne egg exposure. TCDD toxicity in rainbow trout was manifested by half-hatching mortality but predominantly by sac fry mortality associated with hemorrhages, pericardial edema, and yolk sac edema. TCDD LD50s, following injection and waterborne exposure of rainbow trout eggs, were 421 (331–489) and 439 (346–519) pg TCDD/g egg (LD50, 95% fiducial limits), respectively. As in rainbow trout, TCDD toxicity in lake trout (Salvelinus namaycush) following the same two routes of exposure was manifested by half-hatching mortality but predominantly by sac fry mortality preceded by hemorrhages and yolk sac edema. LD50s, based on the dose of TCDD in lake trout eggs, were 47 (21–65) and 65 (60–71) pg/g following injection and waterborne exposure, respectively. The egg injection method is ideal for assessing the relationship between early life stage mortality in rainbow trout and graded egg doses of individual PCDD, PCDF, or PCB congeners. </div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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