Mutagenic treatment induces high transposon variation in barley (Hordeum vulgare L.)
Identifieur interne : 001407 ( Istex/Corpus ); précédent : 001406; suivant : 001408Mutagenic treatment induces high transposon variation in barley (Hordeum vulgare L.)
Auteurs : Kornelia Polok ; Roman ZielinskiSource :
- Acta agriculturae Slovenica [ 1581-9175 ] ; 2011-09-01.
English descriptors
Abstract
With more than 2700 mutant-derived cultivars, mutation techniques belong to the most efficient breeding methods. Despite a relatively good understanding of mutagenesis there is no agreement about the range of genome changes in mutants. Visualizing the gain and the loss of transposon insertion sites in SSAP profiles, the present studies aimed to answer whether or not mutants can be regarded as near isogenic lines with respect to their parent cultivar. Activities of BARE-1 retrotransposon and Tpo1-like DNA transposon from the CACTA superfamily were analysed in ten barley mutants derived by mutagenic treatment of two cultivars, Brenda and Scarlett. A large number and proportion of mutations (on average 21.4 mutations encompassing 19.7% loci) confirm high efficiency of applied mutagens. However, differences exist among both cultivars and transposons. The lack of clear correlation between transposon activities and morphology reflects different mechanisms shaping the mutant architecture. With respect to a transposon type, BARE-1 and Tpo1-like were equally active in inducing mutations. However, their activity differs in that the former was mainly responsible for new insertions while the latter equally for insertions and deletions. An excess of new insertions over recombinational loss suggests a transposon burst as a response to stress caused by chemical mutagens. Low estimations of Nei's similarities, well within the range of semispecies demonstrate the role of mutagenic factors in diversification of populations. This way mutagenic treatment not only provides rough breeding materials but also can be used as a model in evolutionary studies.
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DOI: 10.2478/v10014-011-0012-x
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ISTEX:77B280E8E906A5E052F9A1027621D557B825B9A8Le document en format XML
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However, their activity differs in that the former was mainly responsible for new insertions while the latter equally for insertions and deletions. An excess of new insertions over recombinational loss suggests a transposon burst as a response to stress caused by chemical mutagens. Low estimations of Nei's similarities, well within the range of semispecies demonstrate the role of mutagenic factors in diversification of populations. 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However, their activity differs in that the former was mainly responsible for new insertions while the latter equally for insertions and deletions. An excess of new insertions over recombinational loss suggests a transposon burst as a response to stress caused by chemical mutagens. Low estimations of Nei's similarities, well within the range of semispecies demonstrate the role of mutagenic factors in diversification of populations. 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Despite a relatively good understanding of mutagenesis there is no agreement about the range of genome changes in mutants. Visualizing the gain and the loss of transposon insertion sites in SSAP profiles, the present studies aimed to answer whether or not mutants can be regarded as near isogenic lines with respect to their parent cultivar. Activities of BARE-1 retrotransposon and Tpo1-like DNA transposon from the CACTA superfamily were analysed in ten barley mutants derived by mutagenic treatment of two cultivars, Brenda and Scarlett. A large number and proportion of mutations (on average 21.4 mutations encompassing 19.7% loci) confirm high efficiency of applied mutagens. However, differences exist among both cultivars and transposons. The lack of clear correlation between transposon activities and morphology reflects different mechanisms shaping the mutant architecture. With respect to a transposon type, BARE-1 and Tpo1-like were equally active in inducing mutations. However, their activity differs in that the former was mainly responsible for new insertions while the latter equally for insertions and deletions. An excess of new insertions over recombinational loss suggests a transposon burst as a response to stress caused by chemical mutagens. Low estimations of Nei's similarities, well within the range of semispecies demonstrate the role of mutagenic factors in diversification of populations. This way mutagenic treatment not only provides rough breeding materials but also can be used as a model in evolutionary studies.</p></abstract><abstract xml:lang="sl"><p>Pri več kot 2700 kultivarjih gojenih rastlin so bili mutanti izhodišče za požlahtnitev, torej je induciranje mutacij ena od učinkovitih metod žlahtnjenja rastlin. Kljub razmeroma dobremu razumevanju procesa mutageneze ni soglasja o tem, kakšen je pri mutantih obseg sprememb genoma. S prikazom pridobitve ali izgube na mestih insercije transpozonov pri SSAP profilih poskuša ta raziskava pojasniti, če so mutanti skoro izogene linije v primerjavi z izhodiščnimi kultivarji. Aktivnost retrotranspozona BARE-1 in transpozonu Tpo1 podobnega iz superdružine CACTA je bila raziskana pri desetih mutantih ječmena, dobljenih z mutagenim tretiranjem dveh kultivarjev, Brenda in Scarlett. Veliko število mutacij in njihov delež (v povprečju 21,4 mutacij na 19,7% lokusih) potrjuje učinkovitost uporabljenih mutagenov. Toda med obema kultivarjema in transpozoni so razlike. Ugotovljeno je, da ni jasne povezave med aktivnostjo transpozonov in morfologijo, kar se odraža v razlikah v mehanizmu formiranja oblik pri mutantih. Glede na vrsto transpozona sta BARE-1 in Tpo1-u sličen enako aktivna pri induciranju mutacij. Toda so razlike v aktivnosti glede na to, da prvo navedeni povzroča predvsem nove insercije, medtem ko drugi povzroča tako insercije kot delecije. Višek novih insercij v primerjavi z rekombinacijsko izgubo nakazuje, da je nastanek transpozonov povzročen s stresom, ki ga povzročijo kemični mutageni. Nizka ocena podobnosti po Nei-u, ki je v okviru podobne kot pri podvrstah, kaže na vlogo mutagenih dejavnikov pri diverzifikaciji populacij. Na ta način mutageno obravnavanje ne daje samo izhodiščnega materiala za žlahtnjenje rastlin, ampak je lahko uporabno tudi kot model v evolucijskih raziskavah.</p></abstract><textClass xml:lang="en"><keywords scheme="keyword"><list><head>Keywords</head><item><term>Induced mutants</term></item><item><term>SSAP</term></item><item><term>BARE-1 retrotransposon</term></item><item><term>CACTA transposon</term></item><item><term>genetic similarity</term></item></list></keywords></textClass><textClass xml:lang="sl"><keywords scheme="keyword"><list><head>Keywords</head><item><term>inducirane mutacije</term></item><item><term>SSAP</term></item><item><term>BARE-1 retrotranspozon</term></item><item><term>CACTA transpozon</term></item><item><term>genetska podobnost</term></item></list></keywords></textClass></profileDesc><revisionDesc><change when="2011-11-09">Created</change><change when="2011-09-01">Published</change></revisionDesc></teiHeader></istex:fulltextTEI><json:item><extension>txt</extension><original>false</original><mimetype>text/plain</mimetype><uri>https://api.istex.fr/document/77B280E8E906A5E052F9A1027621D557B825B9A8/fulltext/txt</uri></json:item></fulltext><metadata><istex:metadataXml wicri:clean="corpus degruyter-journals" wicri:toSee="no header"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//NLM//DTD Journal Publishing DTD v3.0 20080202//EN" URI="journalpublishing3.dtd" name="istex:docType"></istex:docType><istex:document><article article-type="research-article" dtd-version="3.0" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">ACAS</journal-id><journal-title-group><abbrev-journal-title abbrev-type="full">Acta agriculturae Slovenica</abbrev-journal-title></journal-title-group><issn pub-type="epub">1854-1941</issn><issn pub-type="ppub">1581-9175</issn><publisher><publisher-name>Versita</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">v10014-011-0012-x</article-id><article-id pub-id-type="doi">10.2478/v10014-011-0012-x</article-id><title-group><article-title>Mutagenic treatment induces high transposon variation in barley (<italic>Hordeum vulgare</italic> L.)</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Polok</surname><given-names>Kornelia</given-names></name><xref ref-type="aff" rid="A1"><sup>1</sup></xref></contrib><contrib contrib-type="author"><name><surname>Zielinski</surname><given-names>Roman</given-names></name><xref ref-type="aff" rid="A1"><sup>1</sup></xref></contrib><aff id="A1">Department of Genetics, University of Warmia and Mazury in Olsztyn, Plac Lodzki 3, 10-967 Olsztyn, Poland<sup>1</sup></aff></contrib-group><author-notes><corresp></corresp></author-notes><pub-date pub-type="ppub"><day>1</day><month>9</month><year>2011</year></pub-date><pub-date pub-type="epub"><day>9</day><month>11</month><year>2011</year></pub-date><volume>97</volume><issue>3</issue><fpage>179</fpage><lpage>188</lpage><permissions><license license-type="open-access"><license-p>This content is open access.</license-p></license></permissions><related-article related-article-type="pdf" xlink:href="v10014-011-0012-x.pdf"></related-article><abstract xml:lang="en"><title>Mutagenic treatment induces high transposon variation in barley (<italic>Hordeum vulgare</italic> L.)</title><p>With more than 2700 mutant-derived cultivars, mutation techniques belong to the most efficient breeding methods. Despite a relatively good understanding of mutagenesis there is no agreement about the range of genome changes in mutants. Visualizing the gain and the loss of transposon insertion sites in SSAP profiles, the present studies aimed to answer whether or not mutants can be regarded as near isogenic lines with respect to their parent cultivar. Activities of <italic>BARE-1</italic> retrotransposon and <italic>Tpo1</italic>-like DNA transposon from the CACTA superfamily were analysed in ten barley mutants derived by mutagenic treatment of two cultivars, Brenda and Scarlett. A large number and proportion of mutations (on average 21.4 mutations encompassing 19.7% loci) confirm high efficiency of applied mutagens. However, differences exist among both cultivars and transposons. The lack of clear correlation between transposon activities and morphology reflects different mechanisms shaping the mutant architecture. With respect to a transposon type, <italic>BARE-1</italic> and <italic>Tpo1</italic>-like were equally active in inducing mutations. However, their activity differs in that the former was mainly responsible for new insertions while the latter equally for insertions and deletions. An excess of new insertions over recombinational loss suggests a transposon burst as a response to stress caused by chemical mutagens. Low estimations of Nei's similarities, well within the range of semispecies demonstrate the role of mutagenic factors in diversification of populations. This way mutagenic treatment not only provides rough breeding materials but also can be used as a model in evolutionary studies.</p></abstract><abstract xml:lang="sl"><title>Mutageno Tretiranje Povzroča Visoko Variabilnost Transpozonov Pri Ječmenu (<italic>Hordeum vulgare</italic> L.)</title><p>Pri več kot 2700 kultivarjih gojenih rastlin so bili mutanti izhodišče za požlahtnitev, torej je induciranje mutacij ena od učinkovitih metod žlahtnjenja rastlin. Kljub razmeroma dobremu razumevanju procesa mutageneze ni soglasja o tem, kakšen je pri mutantih obseg sprememb genoma. S prikazom pridobitve ali izgube na mestih insercije transpozonov pri SSAP profilih poskuša ta raziskava pojasniti, če so mutanti skoro izogene linije v primerjavi z izhodiščnimi kultivarji. Aktivnost retrotranspozona <italic>BARE-1</italic> in transpozonu <italic>Tpo1</italic> podobnega iz superdružine CACTA je bila raziskana pri desetih mutantih ječmena, dobljenih z mutagenim tretiranjem dveh kultivarjev, Brenda in Scarlett. Veliko število mutacij in njihov delež (v povprečju 21,4 mutacij na 19,7% lokusih) potrjuje učinkovitost uporabljenih mutagenov. Toda med obema kultivarjema in transpozoni so razlike. Ugotovljeno je, da ni jasne povezave med aktivnostjo transpozonov in morfologijo, kar se odraža v razlikah v mehanizmu formiranja oblik pri mutantih. Glede na vrsto transpozona sta <italic>BARE-1</italic> in <italic>Tpo1</italic>-u sličen enako aktivna pri induciranju mutacij. Toda so razlike v aktivnosti glede na to, da prvo navedeni povzroča predvsem nove insercije, medtem ko drugi povzroča tako insercije kot delecije. Višek novih insercij v primerjavi z rekombinacijsko izgubo nakazuje, da je nastanek transpozonov povzročen s stresom, ki ga povzročijo kemični mutageni. Nizka ocena podobnosti po Nei-u, ki je v okviru podobne kot pri podvrstah, kaže na vlogo mutagenih dejavnikov pri diverzifikaciji populacij. Na ta način mutageno obravnavanje ne daje samo izhodiščnega materiala za žlahtnjenje rastlin, ampak je lahko uporabno tudi kot model v evolucijskih raziskavah.</p></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Induced mutants</kwd><kwd>SSAP</kwd><kwd><italic>BARE-1</italic> retrotransposon</kwd><kwd>CACTA transposon</kwd><kwd>genetic similarity</kwd></kwd-group><kwd-group xml:lang="sl"><title>Keywords</title><kwd>inducirane mutacije</kwd><kwd>SSAP</kwd><kwd><italic>BARE-1</italic> retrotranspozon</kwd><kwd>CACTA transpozon</kwd><kwd>genetska podobnost</kwd></kwd-group></article-meta></front></article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Mutagenic treatment induces high transposon variation in barley (Hordeum vulgare L.)</title></titleInfo><titleInfo type="alternative" lang="en" contentType="CDATA"><title>Mutagenic treatment induces high transposon variation in barley (Hordeum vulgare L.)</title></titleInfo><name type="personal"><namePart type="given">Kornelia</namePart><namePart type="family">Polok</namePart><affiliation>Department of Genetics, University of Warmia and Mazury in Olsztyn, Plac Lodzki 3, 10-967 Olsztyn, Poland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Roman</namePart><namePart type="family">Zielinski</namePart><affiliation>Department of Genetics, University of Warmia and Mazury in Olsztyn, Plac Lodzki 3, 10-967 Olsztyn, Poland</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="research-article"></genre><originInfo><publisher>Versita</publisher><dateIssued encoding="w3cdtf">2011-09-01</dateIssued><dateCreated encoding="w3cdtf">2011-11-09</dateCreated><copyrightDate encoding="w3cdtf">2011</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">With more than 2700 mutant-derived cultivars, mutation techniques belong to the most efficient breeding methods. Despite a relatively good understanding of mutagenesis there is no agreement about the range of genome changes in mutants. Visualizing the gain and the loss of transposon insertion sites in SSAP profiles, the present studies aimed to answer whether or not mutants can be regarded as near isogenic lines with respect to their parent cultivar. Activities of BARE-1 retrotransposon and Tpo1-like DNA transposon from the CACTA superfamily were analysed in ten barley mutants derived by mutagenic treatment of two cultivars, Brenda and Scarlett. A large number and proportion of mutations (on average 21.4 mutations encompassing 19.7% loci) confirm high efficiency of applied mutagens. However, differences exist among both cultivars and transposons. The lack of clear correlation between transposon activities and morphology reflects different mechanisms shaping the mutant architecture. With respect to a transposon type, BARE-1 and Tpo1-like were equally active in inducing mutations. However, their activity differs in that the former was mainly responsible for new insertions while the latter equally for insertions and deletions. An excess of new insertions over recombinational loss suggests a transposon burst as a response to stress caused by chemical mutagens. Low estimations of Nei's similarities, well within the range of semispecies demonstrate the role of mutagenic factors in diversification of populations. This way mutagenic treatment not only provides rough breeding materials but also can be used as a model in evolutionary studies.</abstract><abstract lang="sl">Pri več kot 2700 kultivarjih gojenih rastlin so bili mutanti izhodišče za požlahtnitev, torej je induciranje mutacij ena od učinkovitih metod žlahtnjenja rastlin. Kljub razmeroma dobremu razumevanju procesa mutageneze ni soglasja o tem, kakšen je pri mutantih obseg sprememb genoma. S prikazom pridobitve ali izgube na mestih insercije transpozonov pri SSAP profilih poskuša ta raziskava pojasniti, če so mutanti skoro izogene linije v primerjavi z izhodiščnimi kultivarji. Aktivnost retrotranspozona BARE-1 in transpozonu Tpo1 podobnega iz superdružine CACTA je bila raziskana pri desetih mutantih ječmena, dobljenih z mutagenim tretiranjem dveh kultivarjev, Brenda in Scarlett. Veliko število mutacij in njihov delež (v povprečju 21,4 mutacij na 19,7% lokusih) potrjuje učinkovitost uporabljenih mutagenov. Toda med obema kultivarjema in transpozoni so razlike. Ugotovljeno je, da ni jasne povezave med aktivnostjo transpozonov in morfologijo, kar se odraža v razlikah v mehanizmu formiranja oblik pri mutantih. Glede na vrsto transpozona sta BARE-1 in Tpo1-u sličen enako aktivna pri induciranju mutacij. Toda so razlike v aktivnosti glede na to, da prvo navedeni povzroča predvsem nove insercije, medtem ko drugi povzroča tako insercije kot delecije. Višek novih insercij v primerjavi z rekombinacijsko izgubo nakazuje, da je nastanek transpozonov povzročen s stresom, ki ga povzročijo kemični mutageni. Nizka ocena podobnosti po Nei-u, ki je v okviru podobne kot pri podvrstah, kaže na vlogo mutagenih dejavnikov pri diverzifikaciji populacij. Na ta način mutageno obravnavanje ne daje samo izhodiščnega materiala za žlahtnjenje rastlin, ampak je lahko uporabno tudi kot model v evolucijskih raziskavah.</abstract><subject lang="en"><genre>Keywords</genre><topic>Induced mutants</topic><topic>SSAP</topic><topic>BARE-1 retrotransposon</topic><topic>CACTA transposon</topic><topic>genetic similarity</topic></subject><subject lang="sl"><genre>Keywords</genre><topic>inducirane mutacije</topic><topic>SSAP</topic><topic>BARE-1 retrotranspozon</topic><topic>CACTA transpozon</topic><topic>genetska podobnost</topic></subject><relatedItem type="host"><titleInfo><title>Acta agriculturae Slovenica</title></titleInfo><genre type="journal">journal</genre><identifier type="ISSN">1581-9175</identifier><identifier type="eISSN">1854-1941</identifier><identifier type="PublisherID">ACAS</identifier><part><date>2011</date><detail type="volume"><caption>vol.</caption><number>97</number></detail><detail type="issue"><caption>no.</caption><number>3</number></detail><extent unit="pages"><start>179</start><end>188</end></extent></part></relatedItem><identifier type="istex">77B280E8E906A5E052F9A1027621D557B825B9A8</identifier><identifier type="DOI">10.2478/v10014-011-0012-x</identifier><identifier type="ArticleID">v10014-011-0012-x</identifier><identifier type="pdf">v10014-011-0012-x.pdf</identifier><accessCondition type="use and reproduction" contentType="open-access">This content is open access.</accessCondition><recordInfo><recordContentSource>De Gruyter</recordContentSource></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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