Inhibition of leukaemia cell proliferation by folic acid-polylysine-mediated introduction of c-myb antisense oligodeoxynucleotides into HL-60 cells.
Identifieur interne : 000141 ( Pmc/Corpus ); précédent : 000140; suivant : 000142Inhibition of leukaemia cell proliferation by folic acid-polylysine-mediated introduction of c-myb antisense oligodeoxynucleotides into HL-60 cells.
Auteurs : G. Citro ; C. Szczylik ; P. Ginobbi ; G. Zupi ; B. CalabrettaSource :
- British Journal of Cancer [ 0007-0920 ] ; 1994.
Abstract
The inhibitory effect of c-myb antisense oligodeoxynucleotides (ODNs) conjugated to folic acid (FA) on HL-60 cell proliferation was examined. Folic acid was covalently linked to a polylysine chain and purified by gel chromatography. Sterile FA-polylysine was complexed with c-myb sense and antisense. Exposure of HL-60 cells to the FA-polylysine-c-myb antisense ODN complex resulted in a down-regulation of c-myb expression and a greater inhibition of proliferation than that obtained using free ODNs. Moreover, FA-polylysine conjugate alone or complexed to c-myb sense ODN was not toxic to cells. The antigenic properties and uptake of the vitamin were not affected by the polylysine chain. These data suggest that this strategy is potentially useful for the selective delivery of anti-oncogene-targeted ODNs into cancer cells.
Url:
PubMed: 8123474
PubMed Central: 1968841
Links to Exploration step
PMC:1968841Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inhibition of leukaemia cell proliferation by folic acid-polylysine-mediated introduction of c-myb antisense oligodeoxynucleotides into HL-60 cells.</title><author><name sortKey="Citro, G" sort="Citro, G" uniqKey="Citro G" first="G." last="Citro">G. Citro</name></author><author><name sortKey="Szczylik, C" sort="Szczylik, C" uniqKey="Szczylik C" first="C." last="Szczylik">C. Szczylik</name></author><author><name sortKey="Ginobbi, P" sort="Ginobbi, P" uniqKey="Ginobbi P" first="P." last="Ginobbi">P. Ginobbi</name></author><author><name sortKey="Zupi, G" sort="Zupi, G" uniqKey="Zupi G" first="G." last="Zupi">G. Zupi</name></author><author><name sortKey="Calabretta, B" sort="Calabretta, B" uniqKey="Calabretta B" first="B." last="Calabretta">B. Calabretta</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">8123474</idno><idno type="pmc">1968841</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1968841</idno><idno type="RBID">PMC:1968841</idno><date when="1994">1994</date><idno type="wicri:Area/Pmc/Corpus">000141</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000141</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Inhibition of leukaemia cell proliferation by folic acid-polylysine-mediated introduction of c-myb antisense oligodeoxynucleotides into HL-60 cells.</title><author><name sortKey="Citro, G" sort="Citro, G" uniqKey="Citro G" first="G." last="Citro">G. Citro</name></author><author><name sortKey="Szczylik, C" sort="Szczylik, C" uniqKey="Szczylik C" first="C." last="Szczylik">C. Szczylik</name></author><author><name sortKey="Ginobbi, P" sort="Ginobbi, P" uniqKey="Ginobbi P" first="P." last="Ginobbi">P. Ginobbi</name></author><author><name sortKey="Zupi, G" sort="Zupi, G" uniqKey="Zupi G" first="G." last="Zupi">G. Zupi</name></author><author><name sortKey="Calabretta, B" sort="Calabretta, B" uniqKey="Calabretta B" first="B." last="Calabretta">B. Calabretta</name></author></analytic><series><title level="j">British Journal of Cancer</title><idno type="ISSN">0007-0920</idno><idno type="eISSN">1532-1827</idno><imprint><date when="1994">1994</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The inhibitory effect of c-myb antisense oligodeoxynucleotides (ODNs) conjugated to folic acid (FA) on HL-60 cell proliferation was examined. Folic acid was covalently linked to a polylysine chain and purified by gel chromatography. Sterile FA-polylysine was complexed with c-myb sense and antisense. Exposure of HL-60 cells to the FA-polylysine-c-myb antisense ODN complex resulted in a down-regulation of c-myb expression and a greater inhibition of proliferation than that obtained using free ODNs. Moreover, FA-polylysine conjugate alone or complexed to c-myb sense ODN was not toxic to cells. The antigenic properties and uptake of the vitamin were not affected by the polylysine chain. These data suggest that this strategy is potentially useful for the selective delivery of anti-oncogene-targeted ODNs into cancer cells.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><label>Figure 2</label><graphic xlink:href="brjcancer00193-0058-a" xlink:role="464"></graphic></fig><fig id="F2"><label>Figure 3</label><graphic xlink:href="brjcancer00193-0059-a" xlink:role="465"></graphic></fig><fig id="F3"><label>Figure 4</label><graphic xlink:href="brjcancer00193-0059-b" xlink:role="465"></graphic></fig><fig id="F4"><label>Figure 6</label><graphic xlink:href="brjcancer00193-0060-a" xlink:role="466"></graphic></fig></sec></div></front></TEI><pmc article-type="research-article"><pmc-dir>properties open_access</pmc-dir>
<front><journal-meta><journal-id journal-id-type="nlm-ta">Br J Cancer</journal-id><journal-title>British Journal of Cancer</journal-title><issn pub-type="ppub">0007-0920</issn><issn pub-type="epub">1532-1827</issn></journal-meta><article-meta><article-id pub-id-type="pmid">8123474</article-id><article-id pub-id-type="pmc">1968841</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title>Inhibition of leukaemia cell proliferation by folic acid-polylysine-mediated introduction of c-myb antisense oligodeoxynucleotides into HL-60 cells.</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Citro</surname><given-names>G.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Szczylik</surname><given-names>C.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Ginobbi</surname><given-names>P.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Zupi</surname><given-names>G.</given-names></name></contrib><contrib contrib-type="author"><name><surname>Calabretta</surname><given-names>B.</given-names></name></contrib></contrib-group><aff>Laboratorio Chemioterapia Sperimentale, Istituto Tumori Regina Elena, Roma, Italy.</aff><pub-date pub-type="ppub"><month>3</month><year>1994</year></pub-date><volume>69</volume><issue>3</issue><fpage>463</fpage><lpage>467</lpage><abstract><p>The inhibitory effect of c-myb antisense oligodeoxynucleotides (ODNs) conjugated to folic acid (FA) on HL-60 cell proliferation was examined. Folic acid was covalently linked to a polylysine chain and purified by gel chromatography. Sterile FA-polylysine was complexed with c-myb sense and antisense. Exposure of HL-60 cells to the FA-polylysine-c-myb antisense ODN complex resulted in a down-regulation of c-myb expression and a greater inhibition of proliferation than that obtained using free ODNs. Moreover, FA-polylysine conjugate alone or complexed to c-myb sense ODN was not toxic to cells. The antigenic properties and uptake of the vitamin were not affected by the polylysine chain. These data suggest that this strategy is potentially useful for the selective delivery of anti-oncogene-targeted ODNs into cancer cells.</p><sec sec-type="scanned-figures"><title>Images</title><fig id="F1"><label>Figure 2</label><graphic xlink:href="brjcancer00193-0058-a" xlink:role="464"></graphic></fig><fig id="F2"><label>Figure 3</label><graphic xlink:href="brjcancer00193-0059-a" xlink:role="465"></graphic></fig><fig id="F3"><label>Figure 4</label><graphic xlink:href="brjcancer00193-0059-b" xlink:role="465"></graphic></fig><fig id="F4"><label>Figure 6</label><graphic xlink:href="brjcancer00193-0060-a" xlink:role="466"></graphic></fig></sec></abstract></article-meta></front><body><supplementary-material content-type="scanned-pages"><graphic xlink:href="brjcancer00193-0057.tif" xlink:title="scanned-page" xlink:role="463" mimetype="image" mime-subtype="tiff"></graphic><graphic xlink:href="brjcancer00193-0058.tif" xlink:title="scanned-page" xlink:role="464" mimetype="image" mime-subtype="tiff"></graphic><graphic xlink:href="brjcancer00193-0059.tif" xlink:title="scanned-page" xlink:role="465" mimetype="image" mime-subtype="tiff"></graphic><graphic xlink:href="brjcancer00193-0060.tif" xlink:title="scanned-page" xlink:role="466" mimetype="image" mime-subtype="tiff"></graphic><graphic xlink:href="brjcancer00193-0061.tif" xlink:title="scanned-page" xlink:role="467" mimetype="image" mime-subtype="tiff"></graphic></supplementary-material></body></pmc></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Informatique/explor/SgmlV1/Data/Pmc/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000141 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd -nk 000141 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Informatique
|area= SgmlV1
|flux= Pmc
|étape= Corpus
|type= RBID
|clé= PMC:1968841
|texte= Inhibition of leukaemia cell proliferation by folic acid-polylysine-mediated introduction of c-myb antisense oligodeoxynucleotides into HL-60 cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Pmc/Corpus/RBID.i -Sk "pubmed:8123474" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Pmc/Corpus/biblio.hfd \
| NlmPubMed2Wicri -a SgmlV1
| This area was generated with Dilib version V0.6.33. |  |