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Immunofluorescence of anti‐actin antibody in gingivai epithelium

Identifieur interne : 004081 ( Main/Merge ); précédent : 004080; suivant : 004082

Immunofluorescence of anti‐actin antibody in gingivai epithelium

Auteurs : David Goteiner [États-Unis] ; Walter S. Krawczyk [États-Unis] ; David G. Gillon [États-Unis]

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RBID : ISTEX:B1130C18633319D405C31F829B059C232C5C43EE

Abstract

The presence of actin in migrating oral epithelial cells during wound healing is being studied. Gingival biopsies of rat oral mucosae were taken at sequential postwounding times and primarily incubated in serum containing an anti‐actin antibody from either actin‐sensitized rabbits or from patients with chronic aggressive hepatitis. Sections were secondarily incubated in fluorescein conjugated goat IgG anti‐rabbit or anti‐human IgG respectively. Controls employed cryostat sectioned rat intestine incubated with anti‐actin antibody as the positive control. The findings from these indirect irnmunofluorescent wound‐healing studies demonstrate a sequential increase in detectable epithelial cell actin during migration and maturation.

Url:
DOI: 10.1111/j.1600-0765.1977.tb00139.x

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ISTEX:B1130C18633319D405C31F829B059C232C5C43EE

Le document en format XML

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<div type="abstract" xml:lang="en">The presence of actin in migrating oral epithelial cells during wound healing is being studied. Gingival biopsies of rat oral mucosae were taken at sequential postwounding times and primarily incubated in serum containing an anti‐actin antibody from either actin‐sensitized rabbits or from patients with chronic aggressive hepatitis. Sections were secondarily incubated in fluorescein conjugated goat IgG anti‐rabbit or anti‐human IgG respectively. Controls employed cryostat sectioned rat intestine incubated with anti‐actin antibody as the positive control. The findings from these indirect irnmunofluorescent wound‐healing studies demonstrate a sequential increase in detectable epithelial cell actin during migration and maturation.</div>
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