Immunofluorescence of anti‐actin antibody in gingivai epithelium
Identifieur interne : 003D68 ( Istex/Checkpoint ); précédent : 003D67; suivant : 003D69Immunofluorescence of anti‐actin antibody in gingivai epithelium
Auteurs : David Goteiner [États-Unis] ; Walter S. Krawczyk [États-Unis] ; David G. Gillon [États-Unis]Source :
- Journal of Periodontal Research [ 0022-3484 ] ; 1977-12.
Abstract
The presence of actin in migrating oral epithelial cells during wound healing is being studied. Gingival biopsies of rat oral mucosae were taken at sequential postwounding times and primarily incubated in serum containing an anti‐actin antibody from either actin‐sensitized rabbits or from patients with chronic aggressive hepatitis. Sections were secondarily incubated in fluorescein conjugated goat IgG anti‐rabbit or anti‐human IgG respectively. Controls employed cryostat sectioned rat intestine incubated with anti‐actin antibody as the positive control. The findings from these indirect irnmunofluorescent wound‐healing studies demonstrate a sequential increase in detectable epithelial cell actin during migration and maturation.
Url:
DOI: 10.1111/j.1600-0765.1977.tb00139.x
Affiliations:
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<author><name sortKey="Krawczyk, Walter S" sort="Krawczyk, Walter S" uniqKey="Krawczyk W" first="Walter S." last="Krawczyk">Walter S. Krawczyk</name>
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<front><div type="abstract" xml:lang="en">The presence of actin in migrating oral epithelial cells during wound healing is being studied. Gingival biopsies of rat oral mucosae were taken at sequential postwounding times and primarily incubated in serum containing an anti‐actin antibody from either actin‐sensitized rabbits or from patients with chronic aggressive hepatitis. Sections were secondarily incubated in fluorescein conjugated goat IgG anti‐rabbit or anti‐human IgG respectively. Controls employed cryostat sectioned rat intestine incubated with anti‐actin antibody as the positive control. The findings from these indirect irnmunofluorescent wound‐healing studies demonstrate a sequential increase in detectable epithelial cell actin during migration and maturation.</div>
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