Neutrophil chemiluminescence in response to Fusobacterium nucleatum
Identifieur interne : 003688 ( Main/Exploration ); précédent : 003687; suivant : 003689Neutrophil chemiluminescence in response to Fusobacterium nucleatum
Auteurs : Samuel A. Passo [États-Unis] ; Salam A. Syed [États-Unis] ; Joseph Silva Jr [États-Unis]Source :
- Journal of Periodontal Research [ 0022-3484 ] ; 1982-12.
Abstract
During the interaction of bacteria or other particles with neutrophils, oxygen consumption is increased, and unstable reduction products are produced. Chemiluminescence is the light energy produced in the neutrophil by the generation of unstable oxygen radicals. These oxygen radicals are thought to be important in the destruction of bacteria as well as host tissues. Therefore, we wanted to investigate the ability of Gram‐negative plaque bacteria to stimulate neutrophil chemiluminescence in vitro. The major groups of bacteria examined were Fusobacterium, Bacteroides, Capnocytophaga. Selenomonas. and Trcponema. Of these bacteria, Fusobacterium nucleatum had by far the greatest ability to stimulate neuirophil chemiluminescence in the absence of serum. Our results suggest that stimulation of neutrophil chemiluminescence by F. nucleatum is mediated by a protein moiety on the bacterial cell surface. This conclusion is supponed by experiments demonstrating significant inhibition of neuirophil chemiluminescence by heat, 2% formalin, or trypsin pre‐treatment of F nucleatum. Also, neutrophil chemiluminescence was stimulated in a similar fashion with intact F. nucleatum only by the cellassociation centrifugation pellet of sonicated F. nucleatum. The monosaccharide D‐galactose. which is a component of erythrocyte glycoproteins and F. nucleatum lipopolysaccharide. caused significant inhibition of neutrophil chemiluminescence at 100 mM final concentration. The unusual and pronounced ability of Fusobacterium nucleatum to stimulate neuirophil chemituminescence in the absence of serum might be important in the pathogenicity and/or host defense in periodontal disease.
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DOI: 10.1111/j.1600-0765.1982.tb01182.x
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Passo</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>The University of Michigan School of Dentistry. School of Medicine and Dental Research Institute, Ann Arbot. Michigan</wicri:regionArea><wicri:noRegion>Ann Arbot. Michigan</wicri:noRegion></affiliation></author><author><name sortKey="Syed, Salam A" sort="Syed, Salam A" uniqKey="Syed S" first="Salam A." last="Syed">Salam A. Syed</name><affiliation wicri:level="1"><country xml:lang="fr">États-Unis</country><wicri:regionArea>The University of Michigan School of Dentistry. School of Medicine and Dental Research Institute, Ann Arbot. Michigan</wicri:regionArea><wicri:noRegion>Ann Arbot. 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Michigan</wicri:noRegion></affiliation></author></analytic><monogr></monogr><series><title level="j">Journal of Periodontal Research</title><idno type="ISSN">0022-3484</idno><idno type="eISSN">1600-0765</idno><imprint><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1982-12">1982-12</date><biblScope unit="volume">17</biblScope><biblScope unit="issue">6</biblScope><biblScope unit="page" from="604">604</biblScope><biblScope unit="page" to="613">613</biblScope></imprint><idno type="ISSN">0022-3484</idno></series><idno type="istex">8D9BC5AC595B3F40F8FB3A6CD4E1F152F19C6F18</idno><idno type="DOI">10.1111/j.1600-0765.1982.tb01182.x</idno><idno type="ArticleID">JRE604</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0022-3484</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">During the interaction of bacteria or other particles with neutrophils, oxygen consumption is increased, and unstable reduction products are produced. Chemiluminescence is the light energy produced in the neutrophil by the generation of unstable oxygen radicals. These oxygen radicals are thought to be important in the destruction of bacteria as well as host tissues. Therefore, we wanted to investigate the ability of Gram‐negative plaque bacteria to stimulate neutrophil chemiluminescence in vitro. The major groups of bacteria examined were Fusobacterium, Bacteroides, Capnocytophaga. Selenomonas. and Trcponema. Of these bacteria, Fusobacterium nucleatum had by far the greatest ability to stimulate neuirophil chemiluminescence in the absence of serum. Our results suggest that stimulation of neutrophil chemiluminescence by F. nucleatum is mediated by a protein moiety on the bacterial cell surface. This conclusion is supponed by experiments demonstrating significant inhibition of neuirophil chemiluminescence by heat, 2% formalin, or trypsin pre‐treatment of F nucleatum. Also, neutrophil chemiluminescence was stimulated in a similar fashion with intact F. nucleatum only by the cellassociation centrifugation pellet of sonicated F. nucleatum. The monosaccharide D‐galactose. which is a component of erythrocyte glycoproteins and F. nucleatum lipopolysaccharide. caused significant inhibition of neutrophil chemiluminescence at 100 mM final concentration. The unusual and pronounced ability of Fusobacterium nucleatum to stimulate neuirophil chemituminescence in the absence of serum might be important in the pathogenicity and/or host defense in periodontal disease.</div></front></TEI><affiliations><list><country><li>États-Unis</li></country></list><tree><country name="États-Unis"><noRegion><name sortKey="Passo, Samuel A" sort="Passo, Samuel A" uniqKey="Passo S" first="Samuel A." last="Passo">Samuel A. Passo</name></noRegion><name sortKey="Silva Jr, Joseph" sort="Silva Jr, Joseph" uniqKey="Silva Jr J" first="Joseph" last="Silva Jr">Joseph Silva Jr</name><name sortKey="Syed, Salam A" sort="Syed, Salam A" uniqKey="Syed S" first="Salam A." last="Syed">Salam A. Syed</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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