Neutrophil chemiluminescence in response to Fusobacterium nucleatum
Identifieur interne : 000D25 ( Istex/Corpus ); précédent : 000D24; suivant : 000D26Neutrophil chemiluminescence in response to Fusobacterium nucleatum
Auteurs : Samuel A. Passo ; Salam A. Syed ; Joseph Silva JrSource :
- Journal of Periodontal Research [ 0022-3484 ] ; 1982-12.
Abstract
During the interaction of bacteria or other particles with neutrophils, oxygen consumption is increased, and unstable reduction products are produced. Chemiluminescence is the light energy produced in the neutrophil by the generation of unstable oxygen radicals. These oxygen radicals are thought to be important in the destruction of bacteria as well as host tissues. Therefore, we wanted to investigate the ability of Gram‐negative plaque bacteria to stimulate neutrophil chemiluminescence in vitro. The major groups of bacteria examined were Fusobacterium, Bacteroides, Capnocytophaga. Selenomonas. and Trcponema. Of these bacteria, Fusobacterium nucleatum had by far the greatest ability to stimulate neuirophil chemiluminescence in the absence of serum. Our results suggest that stimulation of neutrophil chemiluminescence by F. nucleatum is mediated by a protein moiety on the bacterial cell surface. This conclusion is supponed by experiments demonstrating significant inhibition of neuirophil chemiluminescence by heat, 2% formalin, or trypsin pre‐treatment of F nucleatum. Also, neutrophil chemiluminescence was stimulated in a similar fashion with intact F. nucleatum only by the cellassociation centrifugation pellet of sonicated F. nucleatum. The monosaccharide D‐galactose. which is a component of erythrocyte glycoproteins and F. nucleatum lipopolysaccharide. caused significant inhibition of neutrophil chemiluminescence at 100 mM final concentration. The unusual and pronounced ability of Fusobacterium nucleatum to stimulate neuirophil chemituminescence in the absence of serum might be important in the pathogenicity and/or host defense in periodontal disease.
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DOI: 10.1111/j.1600-0765.1982.tb01182.x
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Chemiluminescence is the light energy produced in the neutrophil by the generation of unstable oxygen radicals. These oxygen radicals are thought to be important in the destruction of bacteria as well as host tissues. Therefore, we wanted to investigate the ability of Gram‐negative plaque bacteria to stimulate neutrophil chemiluminescence in vitro. The major groups of bacteria examined were Fusobacterium, Bacteroides, Capnocytophaga. Selenomonas. and Trcponema. Of these bacteria, Fusobacterium nucleatum had by far the greatest ability to stimulate neuirophil chemiluminescence in the absence of serum. Our results suggest that stimulation of neutrophil chemiluminescence by F. nucleatum is mediated by a protein moiety on the bacterial cell surface. This conclusion is supponed by experiments demonstrating significant inhibition of neuirophil chemiluminescence by heat, 2% formalin, or trypsin pre‐treatment of F nucleatum. Also, neutrophil chemiluminescence was stimulated in a similar fashion with intact F. nucleatum only by the cellassociation centrifugation pellet of sonicated F. nucleatum. The monosaccharide D‐galactose. which is a component of erythrocyte glycoproteins and F. nucleatum lipopolysaccharide. caused significant inhibition of neutrophil chemiluminescence at 100 mM final concentration. The unusual and pronounced ability of Fusobacterium nucleatum to stimulate neuirophil chemituminescence in the absence of serum might be important in the pathogenicity and/or host defense in periodontal disease.</div></front></TEI><istex><corpusName>wiley</corpusName><author><json:item><name>Samuel A. Passo</name><affiliations><json:string>The University of Michigan School of Dentistry. School of Medicine and Dental Research Institute, Ann Arbot. Michigan, USA</json:string></affiliations></json:item><json:item><name>Salam A. Syed</name><affiliations><json:string>The University of Michigan School of Dentistry. School of Medicine and Dental Research Institute, Ann Arbot. Michigan, USA</json:string></affiliations></json:item><json:item><name>Joseph Silva Jr</name><affiliations><json:string>The University of Michigan School of Dentistry. School of Medicine and Dental Research Institute, Ann Arbot. Michigan, USA</json:string></affiliations></json:item></author><articleId><json:string>JRE604</json:string></articleId><language><json:string>eng</json:string></language><abstract>During the interaction of bacteria or other particles with neutrophils, oxygen consumption is increased, and unstable reduction products are produced. Chemiluminescence is the light energy produced in the neutrophil by the generation of unstable oxygen radicals. These oxygen radicals are thought to be important in the destruction of bacteria as well as host tissues. Therefore, we wanted to investigate the ability of Gram‐negative plaque bacteria to stimulate neutrophil chemiluminescence in vitro. The major groups of bacteria examined were Fusobacterium, Bacteroides, Capnocytophaga. Selenomonas. and Trcponema. Of these bacteria, Fusobacterium nucleatum had by far the greatest ability to stimulate neuirophil chemiluminescence in the absence of serum. Our results suggest that stimulation of neutrophil chemiluminescence by F. nucleatum is mediated by a protein moiety on the bacterial cell surface. This conclusion is supponed by experiments demonstrating significant inhibition of neuirophil chemiluminescence by heat, 2% formalin, or trypsin pre‐treatment of F nucleatum. Also, neutrophil chemiluminescence was stimulated in a similar fashion with intact F. nucleatum only by the cellassociation centrifugation pellet of sonicated F. nucleatum. The monosaccharide D‐galactose. which is a component of erythrocyte glycoproteins and F. nucleatum lipopolysaccharide. caused significant inhibition of neutrophil chemiluminescence at 100 mM final concentration. 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Also, neutrophil chemiluminescence was stimulated in a similar fashion with intact F. nucleatum only by the cellassociation centrifugation pellet of sonicated F. nucleatum. The monosaccharide D‐galactose. which is a component of erythrocyte glycoproteins and F. nucleatum lipopolysaccharide. caused significant inhibition of neutrophil chemiluminescence at 100 mM final concentration. 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School of Medicine and Dental Research Institute, Ann Arbot. Michigan, USA</unparsedAffiliation></affiliation></affiliationGroup><abstractGroup><abstract type="main" xml:lang="en"><p>During the interaction of bacteria or other particles with neutrophils, oxygen consumption is increased, and unstable reduction products are produced. Chemiluminescence is the light energy produced in the neutrophil by the generation of unstable oxygen radicals. These oxygen radicals are thought to be important in the destruction of bacteria as well as host tissues. Therefore, we wanted to investigate the ability of Gram‐negative plaque bacteria to stimulate neutrophil chemiluminescence <i>in vitro</i>.</p><p>The major groups of bacteria examined were <i>Fusobacterium, Bacteroides, Capnocytophaga. Selenomonas. and Trcponema</i>. Of these bacteria, <i>Fusobacterium nucleatum</i> had by far the greatest ability to stimulate neuirophil chemiluminescence in the absence of serum. Our results suggest that stimulation of neutrophil chemiluminescence by <i>F. nucleatum</i> is mediated by a protein moiety on the bacterial cell surface. This conclusion is supponed by experiments demonstrating significant inhibition of neuirophil chemiluminescence by heat, 2% formalin, or trypsin pre‐treatment of <i>F nucleatum</i>. Also, neutrophil chemiluminescence was stimulated in a similar fashion with intact <i>F. nucleatum</i> only by the cellassociation centrifugation pellet of sonicated <i>F. nucleatum</i>. The monosaccharide D‐galactose. which is a component of erythrocyte glycoproteins and <i>F. nucleatum</i> lipopolysaccharide. caused significant inhibition of neutrophil chemiluminescence at 100 mM final concentration. The unusual and pronounced ability of <i>Fusobacterium nucleatum</i> to stimulate neuirophil chemituminescence in the absence of serum might be important in the pathogenicity and/or host defense in periodontal disease.</p></abstract></abstractGroup></contentMeta></header></component></istex:document></istex:metadataXml><mods version="3.6"><titleInfo lang="en"><title>Neutrophil chemiluminescence in response to Fusobacterium nucleatum</title></titleInfo><titleInfo type="alternative" contentType="CDATA" lang="en"><title>Neutrophil chemiluminescence in response to Fusobacterium nucleatum</title></titleInfo><name type="personal"><namePart type="given">Samuel A.</namePart><namePart type="family">Passo</namePart><affiliation>The University of Michigan School of Dentistry. School of Medicine and Dental Research Institute, Ann Arbot. Michigan, USA</affiliation><description>Correspondence: Department of Oral Pathology School of Dentistry The University of Michigan Ann Arbor. 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Michigan 48109 U.S.A.</description><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="article" displayLabel="article"></genre><originInfo><publisher>Blackwell Publishing Ltd</publisher><place><placeTerm type="text">Oxford, UK</placeTerm></place><dateIssued encoding="w3cdtf">1982-12</dateIssued><edition>(Accepted for publication July 13, 1982)</edition><copyrightDate encoding="w3cdtf">1982</copyrightDate></originInfo><language><languageTerm type="code" authority="rfc3066">en</languageTerm><languageTerm type="code" authority="iso639-2b">eng</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType><extent unit="references">40</extent></physicalDescription><abstract lang="en">During the interaction of bacteria or other particles with neutrophils, oxygen consumption is increased, and unstable reduction products are produced. 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Also, neutrophil chemiluminescence was stimulated in a similar fashion with intact F. nucleatum only by the cellassociation centrifugation pellet of sonicated F. nucleatum. The monosaccharide D‐galactose. which is a component of erythrocyte glycoproteins and F. nucleatum lipopolysaccharide. caused significant inhibition of neutrophil chemiluminescence at 100 mM final concentration. The unusual and pronounced ability of Fusobacterium nucleatum to stimulate neuirophil chemituminescence in the absence of serum might be important in the pathogenicity and/or host defense in periodontal disease.</abstract><relatedItem type="host"><titleInfo><title>Journal of Periodontal Research</title></titleInfo><genre type="Journal">journal</genre><identifier type="ISSN">0022-3484</identifier><identifier type="eISSN">1600-0765</identifier><identifier type="DOI">10.1111/(ISSN)1600-0765</identifier><identifier type="PublisherID">JRE</identifier><part><date>1982</date><detail type="volume"><caption>vol.</caption><number>17</number></detail><detail type="issue"><caption>no.</caption><number>6</number></detail><extent unit="pages"><start>604</start><end>613</end><total>10</total></extent></part></relatedItem><identifier type="istex">8D9BC5AC595B3F40F8FB3A6CD4E1F152F19C6F18</identifier><identifier type="DOI">10.1111/j.1600-0765.1982.tb01182.x</identifier><identifier type="ArticleID">JRE604</identifier><recordInfo><recordContentSource>WILEY</recordContentSource><recordOrigin>Blackwell Publishing Ltd</recordOrigin></recordInfo></mods></metadata><serie></serie></istex></record>Pour manipuler ce document sous Unix (Dilib)
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