Effect of interferon on early enzyme and viral DNA synthesis in vaccinia virus infection
Identifieur interne : 001373 ( Istex/Curation ); précédent : 001372; suivant : 001374Effect of interferon on early enzyme and viral DNA synthesis in vaccinia virus infection
Auteurs : Seymour Levine [États-Unis] ; Wayne E. Magee [États-Unis] ; Ramon D. Hamilton [États-Unis] ; Olga V. Miller [États-Unis]Source :
- Virology [ 0042-6822 ] ; 1967.
Abstract
The quantities of infectious virus and viral DNA-polymerase produced and the rate of viral DNA synthesis were all inversely related to the interferon concentration in vaccinia virus-infected monolayers of whole chicken-embryo fibroblasts. However, virus production was a least 10 times more sensitive to interferon inhibition than were the other parameters studied.Increasing concentrations of interferon depressed the initial rate of synthesis of the DNA polymerase as well as its final yields.The response of the rate of DNA synthesis to interferon concentration was not affected by varying the virus inoculum from 0.1 to 4.0 plaque-forming units per cell.Radioautographic studies of viral DNA synthesis demonstrated that inhibition by interferon is not “all or none” even at the level of the vaccinia inclusion. Instead, increasing concentrations of interferon reduced the quantity of DNA synthesized per site in addition to the proportion of cells initiating DNA synthesis and the number of DNA synthesizing sites per cell. A large proportion of the cells initiated viral DNA synthesis even after treatment with an interferon concentration that reduced virus yield 98%.
Url:
DOI: 10.1016/0042-6822(67)90249-8
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However, virus production was a least 10 times more sensitive to interferon inhibition than were the other parameters studied.Increasing concentrations of interferon depressed the initial rate of synthesis of the DNA polymerase as well as its final yields.The response of the rate of DNA synthesis to interferon concentration was not affected by varying the virus inoculum from 0.1 to 4.0 plaque-forming units per cell.Radioautographic studies of viral DNA synthesis demonstrated that inhibition by interferon is not “all or none” even at the level of the vaccinia inclusion. Instead, increasing concentrations of interferon reduced the quantity of DNA synthesized per site in addition to the proportion of cells initiating DNA synthesis and the number of DNA synthesizing sites per cell. A large proportion of the cells initiated viral DNA synthesis even after treatment with an interferon concentration that reduced virus yield 98%.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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