Effect of interferon on early enzyme and viral DNA synthesis in vaccinia virus infection
Identifieur interne : 001373 ( Istex/Corpus ); précédent : 001372; suivant : 001374Effect of interferon on early enzyme and viral DNA synthesis in vaccinia virus infection
Auteurs : Seymour Levine ; Wayne E. Magee ; Ramon D. Hamilton ; Olga V. MillerSource :
- Virology [ 0042-6822 ] ; 1967.
Abstract
The quantities of infectious virus and viral DNA-polymerase produced and the rate of viral DNA synthesis were all inversely related to the interferon concentration in vaccinia virus-infected monolayers of whole chicken-embryo fibroblasts. However, virus production was a least 10 times more sensitive to interferon inhibition than were the other parameters studied.Increasing concentrations of interferon depressed the initial rate of synthesis of the DNA polymerase as well as its final yields.The response of the rate of DNA synthesis to interferon concentration was not affected by varying the virus inoculum from 0.1 to 4.0 plaque-forming units per cell.Radioautographic studies of viral DNA synthesis demonstrated that inhibition by interferon is not “all or none” even at the level of the vaccinia inclusion. Instead, increasing concentrations of interferon reduced the quantity of DNA synthesized per site in addition to the proportion of cells initiating DNA synthesis and the number of DNA synthesizing sites per cell. A large proportion of the cells initiated viral DNA synthesis even after treatment with an interferon concentration that reduced virus yield 98%.
Url:
DOI: 10.1016/0042-6822(67)90249-8
Links to Exploration step
ISTEX:030AF648F9FB940274192717D43D3D8AD9CFE752Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Effect of interferon on early enzyme and viral DNA synthesis in vaccinia virus infection</title><author><name sortKey="Levine, Seymour" sort="Levine, Seymour" uniqKey="Levine S" first="Seymour" last="Levine">Seymour Levine</name><affiliation><mods:affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</mods:affiliation></affiliation></author><author><name sortKey="Magee, Wayne E" sort="Magee, Wayne E" uniqKey="Magee W" first="Wayne E." last="Magee">Wayne E. Magee</name><affiliation><mods:affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</mods:affiliation></affiliation></author><author><name sortKey="Hamilton, Ramon D" sort="Hamilton, Ramon D" uniqKey="Hamilton R" first="Ramon D." last="Hamilton">Ramon D. Hamilton</name><affiliation><mods:affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</mods:affiliation></affiliation></author><author><name sortKey="Miller, Olga V" sort="Miller, Olga V" uniqKey="Miller O" first="Olga V." last="Miller">Olga V. Miller</name><affiliation><mods:affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</mods:affiliation></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:030AF648F9FB940274192717D43D3D8AD9CFE752</idno><date when="1967" year="1967">1967</date><idno type="doi">10.1016/0042-6822(67)90249-8</idno><idno type="url">https://api.istex.fr/document/030AF648F9FB940274192717D43D3D8AD9CFE752/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">001373</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Effect of interferon on early enzyme and viral DNA synthesis in vaccinia virus infection</title><author><name sortKey="Levine, Seymour" sort="Levine, Seymour" uniqKey="Levine S" first="Seymour" last="Levine">Seymour Levine</name><affiliation><mods:affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</mods:affiliation></affiliation></author><author><name sortKey="Magee, Wayne E" sort="Magee, Wayne E" uniqKey="Magee W" first="Wayne E." last="Magee">Wayne E. Magee</name><affiliation><mods:affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</mods:affiliation></affiliation></author><author><name sortKey="Hamilton, Ramon D" sort="Hamilton, Ramon D" uniqKey="Hamilton R" first="Ramon D." last="Hamilton">Ramon D. Hamilton</name><affiliation><mods:affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</mods:affiliation></affiliation></author><author><name sortKey="Miller, Olga V" sort="Miller, Olga V" uniqKey="Miller O" first="Olga V." last="Miller">Olga V. Miller</name><affiliation><mods:affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</mods:affiliation></affiliation></author></analytic><monogr></monogr><series><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="ISSN">0042-6822</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1967">1967</date><biblScope unit="volume">32</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="33">33</biblScope><biblScope unit="page" to="40">40</biblScope></imprint><idno type="ISSN">0042-6822</idno></series><idno type="istex">030AF648F9FB940274192717D43D3D8AD9CFE752</idno><idno type="DOI">10.1016/0042-6822(67)90249-8</idno><idno type="PII">0042-6822(67)90249-8</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0042-6822</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The quantities of infectious virus and viral DNA-polymerase produced and the rate of viral DNA synthesis were all inversely related to the interferon concentration in vaccinia virus-infected monolayers of whole chicken-embryo fibroblasts. However, virus production was a least 10 times more sensitive to interferon inhibition than were the other parameters studied.Increasing concentrations of interferon depressed the initial rate of synthesis of the DNA polymerase as well as its final yields.The response of the rate of DNA synthesis to interferon concentration was not affected by varying the virus inoculum from 0.1 to 4.0 plaque-forming units per cell.Radioautographic studies of viral DNA synthesis demonstrated that inhibition by interferon is not “all or none” even at the level of the vaccinia inclusion. Instead, increasing concentrations of interferon reduced the quantity of DNA synthesized per site in addition to the proportion of cells initiating DNA synthesis and the number of DNA synthesizing sites per cell. A large proportion of the cells initiated viral DNA synthesis even after treatment with an interferon concentration that reduced virus yield 98%.</div></front></TEI><istex><corpusName>elsevier</corpusName><author><json:item><name>Seymour Levine</name><affiliations><json:string>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</json:string></affiliations></json:item><json:item><name>Wayne E. Magee</name><affiliations><json:string>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</json:string></affiliations></json:item><json:item><name>Ramon D. Hamilton</name><affiliations><json:string>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</json:string></affiliations></json:item><json:item><name>Olga V. Miller</name><affiliations><json:string>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</json:string></affiliations></json:item></author><language><json:string>eng</json:string></language><abstract>The quantities of infectious virus and viral DNA-polymerase produced and the rate of viral DNA synthesis were all inversely related to the interferon concentration in vaccinia virus-infected monolayers of whole chicken-embryo fibroblasts. However, virus production was a least 10 times more sensitive to interferon inhibition than were the other parameters studied.Increasing concentrations of interferon depressed the initial rate of synthesis of the DNA polymerase as well as its final yields.The response of the rate of DNA synthesis to interferon concentration was not affected by varying the virus inoculum from 0.1 to 4.0 plaque-forming units per cell.Radioautographic studies of viral DNA synthesis demonstrated that inhibition by interferon is not “all or none” even at the level of the vaccinia inclusion. Instead, increasing concentrations of interferon reduced the quantity of DNA synthesized per site in addition to the proportion of cells initiating DNA synthesis and the number of DNA synthesizing sites per cell. A large proportion of the cells initiated viral DNA synthesis even after treatment with an interferon concentration that reduced virus yield 98%.</abstract><qualityIndicators><score>4.614</score><pdfVersion>1.3</pdfVersion><pdfPageSize>504 x 720 pts</pdfPageSize><refBibsNative>true</refBibsNative><keywordCount>0</keywordCount><abstractCharCount>1173</abstractCharCount><pdfWordCount>2538</pdfWordCount><pdfCharCount>21455</pdfCharCount><pdfPageCount>8</pdfPageCount><abstractWordCount>173</abstractWordCount></qualityIndicators><title>Effect of interferon on early enzyme and viral DNA synthesis in vaccinia virus infection</title><pii><json:string>0042-6822(67)90249-8</json:string></pii><genre><json:string>research-article</json:string></genre><serie><volume>119</volume><pages><last>553</last><first>551</first></pages><genre></genre><language><json:string>unknown</json:string></language><title>Proc. Soc. Exptl. Biol. Med.</title></serie><host><volume>32</volume><pii><json:string>S0042-6822(00)X0641-1</json:string></pii><pages><last>40</last><first>33</first></pages><issn><json:string>0042-6822</json:string></issn><issue>1</issue><genre><json:string>Journal</json:string></genre><language><json:string>unknown</json:string></language><title>Virology</title><publicationDate>1967</publicationDate></host><categories><wos><json:string>VIROLOGY</json:string></wos></categories><publicationDate>1967</publicationDate><copyrightDate>1967</copyrightDate><doi><json:string>10.1016/0042-6822(67)90249-8</json:string></doi><id>030AF648F9FB940274192717D43D3D8AD9CFE752</id><fulltext><json:item><original>true</original><mimetype>application/pdf</mimetype><extension>pdf</extension><uri>https://api.istex.fr/document/030AF648F9FB940274192717D43D3D8AD9CFE752/fulltext/pdf</uri></json:item><json:item><original>true</original><mimetype>text/plain</mimetype><extension>txt</extension><uri>https://api.istex.fr/document/030AF648F9FB940274192717D43D3D8AD9CFE752/fulltext/txt</uri></json:item><json:item><original>false</original><mimetype>application/zip</mimetype><extension>zip</extension><uri>https://api.istex.fr/document/030AF648F9FB940274192717D43D3D8AD9CFE752/fulltext/zip</uri></json:item><istex:fulltextTEI uri="https://api.istex.fr/document/030AF648F9FB940274192717D43D3D8AD9CFE752/fulltext/tei"><teiHeader><fileDesc><titleStmt><title level="a">Effect of interferon on early enzyme and viral DNA synthesis in vaccinia virus infection</title></titleStmt><publicationStmt><authority>ISTEX</authority><publisher>ELSEVIER</publisher><availability><p>ELSEVIER</p></availability><date>1967</date></publicationStmt><notesStmt><note>Presented in part at the 66th Annual Meeting of the American Society for Microbiology, Los Angeles, California, May 1–5, 1966.</note></notesStmt><sourceDesc><biblStruct type="inbook"><analytic><title level="a">Effect of interferon on early enzyme and viral DNA synthesis in vaccinia virus infection</title><author><persName><forename type="first">Seymour</forename><surname>Levine</surname></persName><affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</affiliation></author><author><persName><forename type="first">Wayne E.</forename><surname>Magee</surname></persName><affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</affiliation></author><author><persName><forename type="first">Ramon D.</forename><surname>Hamilton</surname></persName><affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</affiliation></author><author><persName><forename type="first">Olga V.</forename><surname>Miller</surname></persName><affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</affiliation></author></analytic><monogr><title level="j">Virology</title><title level="j" type="abbrev">YVIRO</title><idno type="pISSN">0042-6822</idno><idno type="PII">S0042-6822(00)X0641-1</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1967"></date><biblScope unit="volume">32</biblScope><biblScope unit="issue">1</biblScope><biblScope unit="page" from="33">33</biblScope><biblScope unit="page" to="40">40</biblScope></imprint></monogr><idno type="istex">030AF648F9FB940274192717D43D3D8AD9CFE752</idno><idno type="DOI">10.1016/0042-6822(67)90249-8</idno><idno type="PII">0042-6822(67)90249-8</idno></biblStruct></sourceDesc></fileDesc><profileDesc><creation><date>1967</date></creation><langUsage><language ident="en">en</language></langUsage><abstract xml:lang="en"><p>The quantities of infectious virus and viral DNA-polymerase produced and the rate of viral DNA synthesis were all inversely related to the interferon concentration in vaccinia virus-infected monolayers of whole chicken-embryo fibroblasts. However, virus production was a least 10 times more sensitive to interferon inhibition than were the other parameters studied.Increasing concentrations of interferon depressed the initial rate of synthesis of the DNA polymerase as well as its final yields.The response of the rate of DNA synthesis to interferon concentration was not affected by varying the virus inoculum from 0.1 to 4.0 plaque-forming units per cell.Radioautographic studies of viral DNA synthesis demonstrated that inhibition by interferon is not “all or none” even at the level of the vaccinia inclusion. Instead, increasing concentrations of interferon reduced the quantity of DNA synthesized per site in addition to the proportion of cells initiating DNA synthesis and the number of DNA synthesizing sites per cell. A large proportion of the cells initiated viral DNA synthesis even after treatment with an interferon concentration that reduced virus yield 98%.</p></abstract></profileDesc><revisionDesc><change when="1967-01-16">Registration</change><change when="1967">Published</change></revisionDesc></teiHeader></istex:fulltextTEI></fulltext><metadata><istex:metadataXml wicri:clean="Elsevier, elements deleted: tail"><istex:xmlDeclaration>version="1.0" encoding="utf-8"</istex:xmlDeclaration><istex:docType PUBLIC="-//ES//DTD journal article DTD version 4.5.2//EN//XML" URI="art452.dtd" name="istex:docType"></istex:docType><istex:document><converted-article version="4.5.2" docsubtype="fla"><item-info><jid>YVIRO</jid><aid>67902498</aid><ce:pii>0042-6822(67)90249-8</ce:pii><ce:doi>10.1016/0042-6822(67)90249-8</ce:doi><ce:copyright type="unknown" year="1967"></ce:copyright></item-info><head><ce:article-footnote><ce:label>☆</ce:label><ce:note-para>Presented in part at the 66th Annual Meeting of the American Society for Microbiology, Los Angeles, California, May 1–5, 1966.</ce:note-para></ce:article-footnote><ce:title>Effect of interferon on early enzyme and viral DNA synthesis in vaccinia virus infection</ce:title><ce:author-group><ce:author><ce:given-name>Seymour</ce:given-name><ce:surname>Levine</ce:surname></ce:author><ce:author><ce:given-name>Wayne E.</ce:given-name><ce:surname>Magee</ce:surname></ce:author><ce:author><ce:given-name>Ramon D.</ce:given-name><ce:surname>Hamilton</ce:surname></ce:author><ce:author><ce:given-name>Olga V.</ce:given-name><ce:surname>Miller</ce:surname></ce:author><ce:affiliation><ce:textfn>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</ce:textfn></ce:affiliation></ce:author-group><ce:date-accepted day="16" month="1" year="1967"></ce:date-accepted><ce:abstract><ce:section-title>Abstract</ce:section-title><ce:abstract-sec><ce:simple-para>The quantities of infectious virus and viral DNA-polymerase produced and the rate of viral DNA synthesis were all inversely related to the interferon concentration in vaccinia virus-infected monolayers of whole chicken-embryo fibroblasts. However, virus production was a least 10 times more sensitive to interferon inhibition than were the other parameters studied.</ce:simple-para><ce:simple-para>Increasing concentrations of interferon depressed the initial rate of synthesis of the DNA polymerase as well as its final yields.</ce:simple-para><ce:simple-para>The response of the rate of DNA synthesis to interferon concentration was not affected by varying the virus inoculum from 0.1 to 4.0 plaque-forming units per cell.</ce:simple-para><ce:simple-para>Radioautographic studies of viral DNA synthesis demonstrated that inhibition by interferon is not “all or none” even at the level of the vaccinia inclusion. Instead, increasing concentrations of interferon reduced the quantity of DNA synthesized per site in addition to the proportion of cells initiating DNA synthesis and the number of DNA synthesizing sites per cell. A large proportion of the cells initiated viral DNA synthesis even after treatment with an interferon concentration that reduced virus yield 98%.</ce:simple-para></ce:abstract-sec></ce:abstract></head></converted-article></istex:document></istex:metadataXml><mods version="3.6"><titleInfo><title>Effect of interferon on early enzyme and viral DNA synthesis in vaccinia virus infection</title></titleInfo><titleInfo type="alternative" contentType="CDATA"><title>Effect of interferon on early enzyme and viral DNA synthesis in vaccinia virus infection</title></titleInfo><name type="personal"><namePart type="given">Seymour</namePart><namePart type="family">Levine</namePart><affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Wayne E.</namePart><namePart type="family">Magee</namePart><affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Ramon D.</namePart><namePart type="family">Hamilton</namePart><affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><name type="personal"><namePart type="given">Olga V.</namePart><namePart type="family">Miller</namePart><affiliation>Virology Research., The Upjohn Company, Kalamazoo, Michigan 49001, USA</affiliation><role><roleTerm type="text">author</roleTerm></role></name><typeOfResource>text</typeOfResource><genre type="research-article" displayLabel="Full-length article"></genre><originInfo><publisher>ELSEVIER</publisher><dateIssued encoding="w3cdtf">1967</dateIssued><dateValid encoding="w3cdtf">1967-01-16</dateValid><copyrightDate encoding="w3cdtf">1967</copyrightDate></originInfo><language><languageTerm type="code" authority="iso639-2b">eng</languageTerm><languageTerm type="code" authority="rfc3066">en</languageTerm></language><physicalDescription><internetMediaType>text/html</internetMediaType></physicalDescription><abstract lang="en">The quantities of infectious virus and viral DNA-polymerase produced and the rate of viral DNA synthesis were all inversely related to the interferon concentration in vaccinia virus-infected monolayers of whole chicken-embryo fibroblasts. However, virus production was a least 10 times more sensitive to interferon inhibition than were the other parameters studied.Increasing concentrations of interferon depressed the initial rate of synthesis of the DNA polymerase as well as its final yields.The response of the rate of DNA synthesis to interferon concentration was not affected by varying the virus inoculum from 0.1 to 4.0 plaque-forming units per cell.Radioautographic studies of viral DNA synthesis demonstrated that inhibition by interferon is not “all or none” even at the level of the vaccinia inclusion. Instead, increasing concentrations of interferon reduced the quantity of DNA synthesized per site in addition to the proportion of cells initiating DNA synthesis and the number of DNA synthesizing sites per cell. A large proportion of the cells initiated viral DNA synthesis even after treatment with an interferon concentration that reduced virus yield 98%.</abstract><note>Presented in part at the 66th Annual Meeting of the American Society for Microbiology, Los Angeles, California, May 1–5, 1966.</note><relatedItem type="host"><titleInfo><title>Virology</title></titleInfo><titleInfo type="abbreviated"><title>YVIRO</title></titleInfo><genre type="Journal">journal</genre><originInfo><dateIssued encoding="w3cdtf">196705</dateIssued></originInfo><identifier type="ISSN">0042-6822</identifier><identifier type="PII">S0042-6822(00)X0641-1</identifier><part><date>196705</date><detail type="volume"><number>32</number><caption>vol.</caption></detail><detail type="issue"><number>1</number><caption>no.</caption></detail><extent unit="issue pages"><start>1</start><end>173</end></extent><extent unit="pages"><start>33</start><end>40</end></extent></part></relatedItem><identifier type="istex">030AF648F9FB940274192717D43D3D8AD9CFE752</identifier><identifier type="DOI">10.1016/0042-6822(67)90249-8</identifier><identifier type="PII">0042-6822(67)90249-8</identifier><recordInfo><recordContentSource>ELSEVIER</recordContentSource></recordInfo></mods></metadata><enrichments><istex:catWosTEI uri="https://api.istex.fr/document/030AF648F9FB940274192717D43D3D8AD9CFE752/enrichments/catWos"><teiHeader><profileDesc><textClass><classCode scheme="WOS">VIROLOGY</classCode></textClass></profileDesc></teiHeader></istex:catWosTEI></enrichments></istex></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Informatique/explor/ScrumV1/Data/Istex/Corpus
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001373 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Corpus/biblio.hfd -nk 001373 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Informatique
|area= ScrumV1
|flux= Istex
|étape= Corpus
|type= RBID
|clé= ISTEX:030AF648F9FB940274192717D43D3D8AD9CFE752
|texte= Effect of interferon on early enzyme and viral DNA synthesis in vaccinia virus infection
}}
| This area was generated with Dilib version V0.6.39. |  |