Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of serum
Identifieur interne : 000835 ( Istex/Curation ); précédent : 000834; suivant : 000836Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of serum
Auteurs : John F. Lechner [États-Unis] ; Aage Haugen [États-Unis] ; Irene A. Mcclendon [États-Unis] ; Abulkalam M. Shamsuddin [États-Unis]Source :
- Differentiation [ 0301-4681 ] ; 1983.
Abstract
Recently we reported a low calcium (110 M) serum-free medium (LHC-1) for clonal growth of normal human bronchial epithelial (NHBE) cells. NHBE cells within colonies are small (mean surface area = 1,250 2) rarely migratory, have few tonofilaments, and multiply with an average population doubling time of 28 h. We have also noted that adding small amounts of blood-derived serum to LHC-1 medium (as little as 2) significantly decreased the clonal growth rate. We have now found that the growth inhibiting effect of serum is due to the induction of squamous (terminal) differentiation. Serum quickly increases the size of the cells (mean surface area = 4,900 2). In addition, the cells acquire numerous desmosomal junctions and an extensive network of keratin bundles. In contrast, human lung carcinoma cells multiply rapidly at clonal density in LHC-1 medium containing as much as 8 serum. Although high concentrations of calcium ions in the medium are known to induce squamous differentiation of epidermal keratinocytes in the absence of serum, high levels of Ca2 (up to 1,000 M) increased the number of desmosomal junctions, but did not significantly affect the clonal growth rate or size of the NHBE cells. However, high concentrations of calcium (above 450 M) were found to potentiate serum differentiation-inducing activity. On the other hand, cholera toxin (10 ng/ml) inhibited the differentiation-inducing acitivy of serum. These results show that squamous differentiation of NHBE cells can be induced by serum and the potency of these serum factors can be modulated. In addition, the data show that lung carcinoma cells differ from their normal counterparts by not undergoing differentiation in the presence of serum.
Url:
DOI: 10.1111/j.1432-0436.1984.tb01361.x
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000835
Links to Exploration step
ISTEX:0AACB1F87C5856C15AAD53899BFD8907734058EALe document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title>Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of serum</title><author><name sortKey="Lechner, John F" sort="Lechner, John F" uniqKey="Lechner J" first="John F." last="Lechner">John F. Lechner</name><affiliation wicri:level="1"><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205</wicri:regionArea></affiliation></author><author><name sortKey="Haugen, Aage" sort="Haugen, Aage" uniqKey="Haugen A" first="Aage" last="Haugen">Aage Haugen</name><affiliation wicri:level="1"><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205</wicri:regionArea></affiliation></author><author><name sortKey="Mcclendon, Irene A" sort="Mcclendon, Irene A" uniqKey="Mcclendon I" first="Irene A." last="Mcclendon">Irene A. Mcclendon</name><affiliation wicri:level="1"><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205</wicri:regionArea></affiliation></author><author><name sortKey="Shamsuddin, Abulkalam M" sort="Shamsuddin, Abulkalam M" uniqKey="Shamsuddin A" first="Abulkalam M." last="Shamsuddin">Abulkalam M. Shamsuddin</name><affiliation wicri:level="1"><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205</wicri:regionArea></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:0AACB1F87C5856C15AAD53899BFD8907734058EA</idno><date when="1984" year="1984">1984</date><idno type="doi">10.1111/j.1432-0436.1984.tb01361.x</idno><idno type="url">https://api.istex.fr/document/0AACB1F87C5856C15AAD53899BFD8907734058EA/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000835</idno><idno type="wicri:Area/Istex/Curation">000835</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a">Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of serum</title><author><name sortKey="Lechner, John F" sort="Lechner, John F" uniqKey="Lechner J" first="John F." last="Lechner">John F. Lechner</name><affiliation wicri:level="1"><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205</wicri:regionArea></affiliation></author><author><name sortKey="Haugen, Aage" sort="Haugen, Aage" uniqKey="Haugen A" first="Aage" last="Haugen">Aage Haugen</name><affiliation wicri:level="1"><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205</wicri:regionArea></affiliation></author><author><name sortKey="Mcclendon, Irene A" sort="Mcclendon, Irene A" uniqKey="Mcclendon I" first="Irene A." last="Mcclendon">Irene A. Mcclendon</name><affiliation wicri:level="1"><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205</wicri:regionArea></affiliation></author><author><name sortKey="Shamsuddin, Abulkalam M" sort="Shamsuddin, Abulkalam M" uniqKey="Shamsuddin A" first="Abulkalam M." last="Shamsuddin">Abulkalam M. Shamsuddin</name><affiliation wicri:level="1"><mods:affiliation>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205, USA</mods:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Laboratory of Human Carcinogenesis, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA, 20205</wicri:regionArea></affiliation></author></analytic><monogr></monogr><series><title level="j">Differentiation</title><title level="j" type="abbrev">DIFF</title><idno type="ISSN">0301-4681</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1983">1983</date><biblScope unit="volume">25</biblScope><biblScope unit="issue">1–3</biblScope><biblScope unit="page" from="229">229</biblScope><biblScope unit="page" to="237">237</biblScope></imprint><idno type="ISSN">0301-4681</idno></series><idno type="istex">0AACB1F87C5856C15AAD53899BFD8907734058EA</idno><idno type="DOI">10.1111/j.1432-0436.1984.tb01361.x</idno><idno type="PII">S0301-4681(11)61612-5</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0301-4681</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recently we reported a low calcium (110 M) serum-free medium (LHC-1) for clonal growth of normal human bronchial epithelial (NHBE) cells. NHBE cells within colonies are small (mean surface area = 1,250 2) rarely migratory, have few tonofilaments, and multiply with an average population doubling time of 28 h. We have also noted that adding small amounts of blood-derived serum to LHC-1 medium (as little as 2) significantly decreased the clonal growth rate. We have now found that the growth inhibiting effect of serum is due to the induction of squamous (terminal) differentiation. Serum quickly increases the size of the cells (mean surface area = 4,900 2). In addition, the cells acquire numerous desmosomal junctions and an extensive network of keratin bundles. In contrast, human lung carcinoma cells multiply rapidly at clonal density in LHC-1 medium containing as much as 8 serum. Although high concentrations of calcium ions in the medium are known to induce squamous differentiation of epidermal keratinocytes in the absence of serum, high levels of Ca2 (up to 1,000 M) increased the number of desmosomal junctions, but did not significantly affect the clonal growth rate or size of the NHBE cells. However, high concentrations of calcium (above 450 M) were found to potentiate serum differentiation-inducing activity. On the other hand, cholera toxin (10 ng/ml) inhibited the differentiation-inducing acitivy of serum. These results show that squamous differentiation of NHBE cells can be induced by serum and the potency of these serum factors can be modulated. In addition, the data show that lung carcinoma cells differ from their normal counterparts by not undergoing differentiation in the presence of serum.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Informatique/explor/ScrumV1/Data/Istex/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000835 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Istex/Curation/biblio.hfd -nk 000835 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Informatique
|area= ScrumV1
|flux= Istex
|étape= Curation
|type= RBID
|clé= ISTEX:0AACB1F87C5856C15AAD53899BFD8907734058EA
|texte= Induction of squamous differentiation of normal human bronchial epithelial cells by small amounts of serum
}}
| This area was generated with Dilib version V0.6.39. |  |